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P13035 (GLPD_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 133. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Aerobic glycerol-3-phosphate dehydrogenase

EC=1.1.5.3
Gene names
Name:glpD
Synonyms:glyD
Ordered Locus Names:b3426, JW3389
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length501 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Conversion of glycerol 3-phosphate to dihydroxyacetone. Uses molecular oxygen or nitrate as electron acceptor.

Catalytic activity

sn-glycerol 3-phosphate + a quinone = glycerone phosphate + a quinol.

Cofactor

FAD.

Pathway

Polyol metabolism; glycerol degradation via glycerol kinase pathway; glycerone phosphate from sn-glycerol 3-phosphate (aerobic route): step 1/1.

Subcellular location

Cytoplasm.

Miscellaneous

There are two sn-glycerol-3-phosphate dehydrogenase isozymes in E.coli: one is aerobic, the other anaerobic.

Sequence similarities

Belongs to the FAD-dependent glycerol-3-phosphate dehydrogenase family.

Sequence caution

The sequence AAA23888.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 501501Aerobic glycerol-3-phosphate dehydrogenase
PRO_0000126098

Regions

Nucleotide binding5 – 3329FAD Potential

Experimental info

Sequence conflict231A → G in BAA00327. Ref.2
Sequence conflict328 – 35326QAITR…SVFGG → HVLPVITPLIFMMKMAKHRC CRYSAA in BAA00327. Ref.2
Sequence conflict464 – 50138DDALW…LSLAS → ARRPVASHKTRHVAKCGSTI SCESVAGGVYAAEVIAGVVN in BAA00327. Ref.2

Secondary structure

.................................................................................................. 501
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P13035 [UniParc].

Last modified May 1, 1992. Version 3.
Checksum: 1C4D341E9E4536AB

FASTA50156,751
        10         20         30         40         50         60 
METKDLIVIG GGINGAGIAA DAAGRGLSVL MLEAQDLACA TSSASSKLIH GGLRYLEHYE 

        70         80         90        100        110        120 
FRLVSEALAE REVLLKMAPH IAFPMRFRLP HRPHLRPAWM IRIGLFMYDH LGKRTSLPGS 

       130        140        150        160        170        180 
TGLRFGANSV LKPEIKRGFE YSDCWVDDAR LVLANAQMVV RKGGEVLTRT RATSARRENG 

       190        200        210        220        230        240 
LWIVEAEDID TGKKYSWQAR GLVNATGPWV KQFFDDGMHL PSPYGIRLIK GSHIVVPRVH 

       250        260        270        280        290        300 
TQKQAYILQN EDKRIVFVIP WMDEFSIIGT TDVEYKGDPK AVKIEESEIN YLLNVYNTHF 

       310        320        330        340        350        360 
KKQLSRDDIV WTYSGVRPLC DDESDSPQAI TRDYTLDIHD ENGKAPLLSV FGGKLTTYRK 

       370        380        390        400        410        420 
LAEHALEKLT PYYQGIGPAW TKESVLPGGA IEGDRDDYAA RLRRRYPFLT ESLARHYART 

       430        440        450        460        470        480 
YGSNSELLLG NAGTVSDLGE DFGHEFYEAE LKYLVDHEWV RRADDALWRR TKQGMWLNAD 

       490        500 
QQSRVSQWLV EYTQQRLSLA S 

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence of the glpD gene encoding aerobic sn-glycerol 3-phosphate dehydrogenase of Escherichia coli K-12."
Austin D., Larson T.J.
J. Bacteriol. 173:101-107(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12.
[2]"Nucleotide sequence of the glycerol-3-phosphate dehydrogenase gene of Escherichia coli and regulation by the cAMP-CRP complex."
Choi Y.-L., Kawase S., Kawamukai M., Utsumi R., Sakai H., Komano T.
Agric. Biol. Chem. 53:1135-1143(1989)
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]"Structures of the promoter and operator of the glpD gene encoding aerobic sn-glycerol-3-phosphate dehydrogenase of Escherichia coli K-12."
Ye S., Larson T.J.
J. Bacteriol. 170:4209-4215(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-61.
Strain: K12.
[6]"Nucleotide sequence of the glpR gene encoding the repressor for the glycerol-3-phosphate regulon of Escherichia coli K12."
Choi Y.-L., Kawase S., Nishida T., Sakai H., Komano T., Kawamukai M., Utsumi R., Kohara Y., Akiyama K.
Nucleic Acids Res. 16:7732-7732(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-61.
[7]"Escherichia coli proteome analysis using the gene-protein database."
VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.
Electrophoresis 18:1243-1251(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY 2D-GEL.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M55989 Genomic DNA. Translation: AAA24636.1.
M96795 Genomic DNA. Translation: AAC28164.1.
D00425 Genomic DNA. Translation: BAA00327.1.
U18997 Genomic DNA. Translation: AAA58224.1.
U00096 Genomic DNA. Translation: AAC76451.1.
AP009048 Genomic DNA. Translation: BAE77866.1.
M21277 Genomic DNA. Translation: AAA23885.1.
M54940 Genomic DNA. Translation: AAA23888.1. Different initiation.
PIRDEECGD. A39186.
RefSeqNP_417884.1. NC_000913.3.
YP_492007.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2QCUX-ray1.75A/B1-501[»]
2R45X-ray2.30A/B1-501[»]
2R46X-ray2.10A/B1-501[»]
2R4EX-ray2.10A/B1-501[»]
2R4JX-ray1.96A/B1-501[»]
ProteinModelPortalP13035.
SMRP13035. Positions 1-501.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-9793N.
IntActP13035. 90 interactions.
MINTMINT-1248675.
STRING511145.b3426.

2D gel databases

SWISS-2DPAGEP13035.

Proteomic databases

PaxDbP13035.
PRIDEP13035.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC76451; AAC76451; b3426.
BAE77866; BAE77866; BAE77866.
GeneID12932271.
947934.
KEGGecj:Y75_p3751.
eco:b3426.
PATRIC32122288. VBIEscCol129921_3521.

Organism-specific databases

EchoBASEEB0389.
EcoGeneEG10394. glpD.

Phylogenomic databases

eggNOGCOG0578.
HOGENOMHOG000004811.
KOK00111.
OMAHAMEKLS.
OrthoDBEOG651SR7.
PhylomeDBP13035.
ProtClustDBPRK12266.

Enzyme and pathway databases

BioCycEcoCyc:AERGLYC3PDEHYDROG-MONOMER.
ECOL316407:JW3389-MONOMER.
MetaCyc:AERGLYC3PDEHYDROG-MONOMER.
UniPathwayUPA00618; UER00674.

Gene expression databases

GenevestigatorP13035.

Family and domain databases

InterProIPR006076. FAD-dep_OxRdtase.
IPR000447. G3P_DH_FAD-dep.
[Graphical view]
PfamPF01266. DAO. 1 hit.
[Graphical view]
PRINTSPR01001. FADG3PDH.
PROSITEPS00977. FAD_G3PDH_1. 1 hit.
PS00978. FAD_G3PDH_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP13035.
PROP13035.

Entry information

Entry nameGLPD_ECOLI
AccessionPrimary (citable) accession number: P13035
Secondary accession number(s): P78115, Q2M790, Q47234
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: May 1, 1992
Last modified: April 16, 2014
This is version 133 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

PATHWAY comments

Index of metabolic and biosynthesis pathways

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene