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P13033

- GLPB_ECOLI

UniProt

P13033 - GLPB_ECOLI

Protein

Anaerobic glycerol-3-phosphate dehydrogenase subunit B

Gene

glpB

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 131 (01 Oct 2014)
      Sequence version 1 (01 Jan 1990)
      Previous versions | rss
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    Functioni

    Conversion of glycerol 3-phosphate to dihydroxyacetone. Uses fumarate or nitrate as electron acceptor.

    Catalytic activityi

    sn-glycerol 3-phosphate + a quinone = glycerone phosphate + a quinol.

    Cofactori

    FMN.

    Pathwayi

    GO - Molecular functioni

    1. glycerol-3-phosphate dehydrogenase activity Source: EcoCyc
    2. sn-glycerol-3-phosphate:ubiquinone-8 oxidoreductase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glycerol catabolic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    Flavoprotein, FMN

    Enzyme and pathway databases

    BioCyciEcoCyc:ANGLYC3PDEHYDROGSUBUNITB-MONOMER.
    ECOL316407:JW2236-MONOMER.
    MetaCyc:ANGLYC3PDEHYDROGSUBUNITB-MONOMER.
    UniPathwayiUPA00618; UER00673.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Anaerobic glycerol-3-phosphate dehydrogenase subunit B (EC:1.1.5.3)
    Short name:
    Anaerobic G-3-P dehydrogenase subunit B
    Short name:
    Anaerobic G3Pdhase B
    Gene namesi
    Name:glpB
    Ordered Locus Names:b2242, JW2236
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10392. glpB.

    Subcellular locationi

    Cell inner membrane; Peripheral membrane protein
    Note: Loosely bound to the cytoplasmic membrane often occurring in vesicles associated with fumarate reductase.

    GO - Cellular componenti

    1. plasma membrane Source: EcoCyc

    Keywords - Cellular componenti

    Cell inner membrane, Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 419419Anaerobic glycerol-3-phosphate dehydrogenase subunit BPRO_0000204559Add
    BLAST

    Proteomic databases

    PaxDbiP13033.
    PRIDEiP13033.

    Expressioni

    Gene expression databases

    GenevestigatoriP13033.

    Interactioni

    Subunit structurei

    Composed of a catalytic GlpA/B dimer and of membrane bound GlpC.

    Protein-protein interaction databases

    DIPiDIP-9791N.
    IntActiP13033. 5 interactions.
    MINTiMINT-1319997.
    STRINGi511145.b2242.

    Structurei

    3D structure databases

    ProteinModelPortaliP13033.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG3075.
    HOGENOMiHOG000278489.
    KOiK00112.
    OMAiFRSVNIS.
    OrthoDBiEOG6K6V62.
    PhylomeDBiP13033.

    Family and domain databases

    HAMAPiMF_00753. Glycerol3P_GlpB.
    InterProiIPR003953. FAD_bind_dom.
    IPR009158. G3P_DH_GlpB_su.
    [Graphical view]
    PfamiPF00890. FAD_binding_2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000141. Anaerobic_G3P_dh. 1 hit.
    TIGRFAMsiTIGR03378. glycerol3P_GlpB. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P13033-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRFDTVIMGG GLAGLLCGLQ LQKHGLRCAI VTRGQSALHF SSGSLDLLSH    50
    LPDGQPVTDI HSGLESLRQQ APAHPYSLLE PQRVLDLACQ AQALIAESGA 100
    QLQGSVELAH QRVTPLGTLR STWLSSPEVP VWPLPAKKIC VVGISGLMDF 150
    QAHLAAASLR ELGLAVETAE IELPELDVLR NNATEFRAVN IARFLDNEEN 200
    WPLLLDALIP VANTCEMILM PACFGLADDK LWRWLNEKLP CSLMLLPTLP 250
    PSVLGIRLQN QLQRQFVRQG GVWMPGDEVK KVTCKNGVVN EIWTRNHADI 300
    PLRPRFAVLA SGSFFSGGLV AERNGIREPI LGLDVLQTAT RGEWYKGDFF 350
    APQPWQQFGV TTDETLRPSQ AGQTIENLFA IGSVLGGFDP IAQGCGGGVC 400
    AVSALHAAQQ IAQRAGGQQ 419
    Length:419
    Mass (Da):45,357
    Last modified:January 1, 1990 - v1
    Checksum:iC8A2285AD09F4F55
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M20938 Genomic DNA. Translation: AAA83865.1.
    U00096 Genomic DNA. Translation: AAC75302.1.
    AP009048 Genomic DNA. Translation: BAA16061.1.
    PIRiB32006. DEECNB.
    RefSeqiNP_416745.1. NC_000913.3.
    YP_490481.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC75302; AAC75302; b2242.
    BAA16061; BAA16061; BAA16061.
    GeneIDi12931496.
    946733.
    KEGGiecj:Y75_p2204.
    eco:b2242.
    PATRICi32119843. VBIEscCol129921_2331.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M20938 Genomic DNA. Translation: AAA83865.1 .
    U00096 Genomic DNA. Translation: AAC75302.1 .
    AP009048 Genomic DNA. Translation: BAA16061.1 .
    PIRi B32006. DEECNB.
    RefSeqi NP_416745.1. NC_000913.3.
    YP_490481.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P13033.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-9791N.
    IntActi P13033. 5 interactions.
    MINTi MINT-1319997.
    STRINGi 511145.b2242.

    Proteomic databases

    PaxDbi P13033.
    PRIDEi P13033.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC75302 ; AAC75302 ; b2242 .
    BAA16061 ; BAA16061 ; BAA16061 .
    GeneIDi 12931496.
    946733.
    KEGGi ecj:Y75_p2204.
    eco:b2242.
    PATRICi 32119843. VBIEscCol129921_2331.

    Organism-specific databases

    EchoBASEi EB0387.
    EcoGenei EG10392. glpB.

    Phylogenomic databases

    eggNOGi COG3075.
    HOGENOMi HOG000278489.
    KOi K00112.
    OMAi FRSVNIS.
    OrthoDBi EOG6K6V62.
    PhylomeDBi P13033.

    Enzyme and pathway databases

    UniPathwayi UPA00618 ; UER00673 .
    BioCyci EcoCyc:ANGLYC3PDEHYDROGSUBUNITB-MONOMER.
    ECOL316407:JW2236-MONOMER.
    MetaCyc:ANGLYC3PDEHYDROGSUBUNITB-MONOMER.

    Miscellaneous databases

    PROi P13033.

    Gene expression databases

    Genevestigatori P13033.

    Family and domain databases

    HAMAPi MF_00753. Glycerol3P_GlpB.
    InterProi IPR003953. FAD_bind_dom.
    IPR009158. G3P_DH_GlpB_su.
    [Graphical view ]
    Pfami PF00890. FAD_binding_2. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000141. Anaerobic_G3P_dh. 1 hit.
    TIGRFAMsi TIGR03378. glycerol3P_GlpB. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence and gene-polypeptide relationships of the glpABC operon encoding the anaerobic sn-glycerol-3-phosphate dehydrogenase of Escherichia coli K-12."
      Cole S.T., Eiglmeier K., Ahmed S., Honore N., Elmes L., Anderson W.F., Weiner J.H.
      J. Bacteriol. 170:2448-2456(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-10.
      Strain: K12.
    2. "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features."
      Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T.
      , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.
      DNA Res. 4:91-113(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

    Entry informationi

    Entry nameiGLPB_ECOLI
    AccessioniPrimary (citable) accession number: P13033
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1990
    Last sequence update: January 1, 1990
    Last modified: October 1, 2014
    This is version 131 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3