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P13033 (GLPB_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 130. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Anaerobic glycerol-3-phosphate dehydrogenase subunit B

Short name=Anaerobic G-3-P dehydrogenase subunit B
Short name=Anaerobic G3Pdhase B
EC=1.1.5.3
Gene names
Name:glpB
Ordered Locus Names:b2242, JW2236
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length419 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Conversion of glycerol 3-phosphate to dihydroxyacetone. Uses fumarate or nitrate as electron acceptor. HAMAP-Rule MF_00753

Catalytic activity

sn-glycerol 3-phosphate + a quinone = glycerone phosphate + a quinol. HAMAP-Rule MF_00753

Cofactor

FMN.

Pathway

Polyol metabolism; glycerol degradation via glycerol kinase pathway; glycerone phosphate from sn-glycerol 3-phosphate (anaerobic route): step 1/1. HAMAP-Rule MF_00753

Subunit structure

Composed of a catalytic GlpA/B dimer and of membrane bound GlpC.

Subcellular location

Cell inner membrane; Peripheral membrane protein. Note: Loosely bound to the cytoplasmic membrane often occurring in vesicles associated with fumarate reductase. HAMAP-Rule MF_00753

Sequence similarities

Belongs to the anaerobic G-3-P dehydrogenase subunit B family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 419419Anaerobic glycerol-3-phosphate dehydrogenase subunit B HAMAP-Rule MF_00753
PRO_0000204559

Sequences

Sequence LengthMass (Da)Tools
P13033 [UniParc].

Last modified January 1, 1990. Version 1.
Checksum: C8A2285AD09F4F55

FASTA41945,357
        10         20         30         40         50         60 
MRFDTVIMGG GLAGLLCGLQ LQKHGLRCAI VTRGQSALHF SSGSLDLLSH LPDGQPVTDI 

        70         80         90        100        110        120 
HSGLESLRQQ APAHPYSLLE PQRVLDLACQ AQALIAESGA QLQGSVELAH QRVTPLGTLR 

       130        140        150        160        170        180 
STWLSSPEVP VWPLPAKKIC VVGISGLMDF QAHLAAASLR ELGLAVETAE IELPELDVLR 

       190        200        210        220        230        240 
NNATEFRAVN IARFLDNEEN WPLLLDALIP VANTCEMILM PACFGLADDK LWRWLNEKLP 

       250        260        270        280        290        300 
CSLMLLPTLP PSVLGIRLQN QLQRQFVRQG GVWMPGDEVK KVTCKNGVVN EIWTRNHADI 

       310        320        330        340        350        360 
PLRPRFAVLA SGSFFSGGLV AERNGIREPI LGLDVLQTAT RGEWYKGDFF APQPWQQFGV 

       370        380        390        400        410 
TTDETLRPSQ AGQTIENLFA IGSVLGGFDP IAQGCGGGVC AVSALHAAQQ IAQRAGGQQ 

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence and gene-polypeptide relationships of the glpABC operon encoding the anaerobic sn-glycerol-3-phosphate dehydrogenase of Escherichia coli K-12."
Cole S.T., Eiglmeier K., Ahmed S., Honore N., Elmes L., Anderson W.F., Weiner J.H.
J. Bacteriol. 170:2448-2456(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-10.
Strain: K12.
[2]"Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features."
Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T. expand/collapse author list , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.
DNA Res. 4:91-113(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M20938 Genomic DNA. Translation: AAA83865.1.
U00096 Genomic DNA. Translation: AAC75302.1.
AP009048 Genomic DNA. Translation: BAA16061.1.
PIRDEECNB. B32006.
RefSeqNP_416745.1. NC_000913.3.
YP_490481.1. NC_007779.1.

3D structure databases

ProteinModelPortalP13033.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-9791N.
IntActP13033. 5 interactions.
MINTMINT-1319997.
STRING511145.b2242.

Proteomic databases

PaxDbP13033.
PRIDEP13033.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC75302; AAC75302; b2242.
BAA16061; BAA16061; BAA16061.
GeneID12931496.
946733.
KEGGecj:Y75_p2204.
eco:b2242.
PATRIC32119843. VBIEscCol129921_2331.

Organism-specific databases

EchoBASEEB0387.
EcoGeneEG10392. glpB.

Phylogenomic databases

eggNOGCOG3075.
HOGENOMHOG000278489.
KOK00112.
OMAFRSVNIS.
OrthoDBEOG6K6V62.
PhylomeDBP13033.

Enzyme and pathway databases

BioCycEcoCyc:ANGLYC3PDEHYDROGSUBUNITB-MONOMER.
ECOL316407:JW2236-MONOMER.
MetaCyc:ANGLYC3PDEHYDROGSUBUNITB-MONOMER.
UniPathwayUPA00618; UER00673.

Gene expression databases

GenevestigatorP13033.

Family and domain databases

HAMAPMF_00753. Glycerol3P_GlpB.
InterProIPR003953. FAD_bind_dom.
IPR009158. G3P_DH_GlpB_su.
[Graphical view]
PfamPF00890. FAD_binding_2. 1 hit.
[Graphical view]
PIRSFPIRSF000141. Anaerobic_G3P_dh. 1 hit.
TIGRFAMsTIGR03378. glycerol3P_GlpB. 1 hit.
ProtoNetSearch...

Other

PROP13033.

Entry information

Entry nameGLPB_ECOLI
AccessionPrimary (citable) accession number: P13033
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: June 11, 2014
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene