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P13016

- AMPD_ECOLI

UniProt

P13016 - AMPD_ECOLI

Protein

1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD

Gene

ampD

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 114 (01 Oct 2014)
      Sequence version 1 (01 Jan 1990)
      Previous versions | rss
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    Functioni

    Involved in both cell wall peptidoglycans recycling and beta-lactamase induction. Specifically cleaves the amide bond between the lactyl group of N-acetylmuramic acid and the alpha-amino group of the L-alanine in degradation products containing an anhydro N-acetylmuramyl moiety By similarity.By similarity

    Catalytic activityi

    Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.

    Cofactori

    Zinc; required for amidase activity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi34 – 341Zinc; catalyticBy similarity
    Active sitei116 – 1161Proton acceptorBy similarity
    Metal bindingi154 – 1541Zinc; catalyticBy similarity
    Sitei162 – 1621Transition state stabilizerBy similarity
    Metal bindingi164 – 1641Zinc; catalyticBy similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. N-acetylmuramoyl-L-alanine amidase activity Source: EcoCyc

    GO - Biological processi

    1. peptidoglycan catabolic process Source: InterPro
    2. peptidoglycan turnover Source: EcoCyc

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Cell wall biogenesis/degradation

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciEcoCyc:EG10041-MONOMER.
    ECOL316407:JW0106-MONOMER.
    MetaCyc:EG10041-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD (EC:3.5.1.28)
    Alternative name(s):
    N-acetylmuramoyl-L-alanine amidase
    Gene namesi
    Name:ampD
    Ordered Locus Names:b0110, JW0106
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10041. ampD.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 1831831,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpDPRO_0000164413Add
    BLAST

    Proteomic databases

    PRIDEiP13016.

    Expressioni

    Inductioni

    Inactivation of AmpD results in AmpR-dependent hyperinduction and AmpD affects beta-lactam susceptibility in the absence of cloned C.freundii amp genes suggesting a linkage between AmpD and peptidoglycan synthesis.

    Gene expression databases

    GenevestigatoriP13016.

    Interactioni

    Protein-protein interaction databases

    STRINGi511145.b0110.

    Structurei

    3D structure databases

    ProteinModelPortaliP13016.
    SMRiP13016. Positions 1-179.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG3023.
    HOGENOMiHOG000255963.
    KOiK03806.
    OMAiGRPRCND.
    OrthoDBiEOG60GRR5.
    PhylomeDBiP13016.

    Family and domain databases

    Gene3Di3.40.80.10. 1 hit.
    InterProiIPR002502. Amidase_domain.
    [Graphical view]
    PfamiPF01510. Amidase_2. 1 hit.
    [Graphical view]
    SMARTiSM00644. Ami_2. 1 hit.
    [Graphical view]
    SUPFAMiSSF55846. SSF55846. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P13016-1 [UniParc]FASTAAdd to Basket

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    MLLEQGWLVG ARRVPSPHYD CRPDDETPTL LVVHNISLPP GEFGGPWIDA    50
    LFTGTIDPQA HPFFAEIAHL RVSAHCLIRR DGEIVQYVPF DKRAWHAGVS 100
    QYQGRERCND FSIGIELEGT DTLAYTDAQY QQLAAVTRAL IDCYPDIAKN 150
    MTGHCDIAPD RKTDPGPAFD WARFRVLVSK ETT 183
    Length:183
    Mass (Da):20,536
    Last modified:January 1, 1990 - v1
    Checksum:iF62910F8F889043D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X15237 Genomic DNA. Translation: CAA33314.1.
    U00096 Genomic DNA. Translation: AAC73221.1.
    AP009048 Genomic DNA. Translation: BAB96679.1.
    L28105 Genomic DNA. Translation: AAC36921.1.
    PIRiS05569.
    RefSeqiNP_414652.1. NC_000913.3.
    YP_488413.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC73221; AAC73221; b0110.
    BAB96679; BAB96679; BAB96679.
    GeneIDi12932901.
    948877.
    KEGGiecj:Y75_p0107.
    eco:b0110.
    PATRICi32115321. VBIEscCol129921_0112.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X15237 Genomic DNA. Translation: CAA33314.1 .
    U00096 Genomic DNA. Translation: AAC73221.1 .
    AP009048 Genomic DNA. Translation: BAB96679.1 .
    L28105 Genomic DNA. Translation: AAC36921.1 .
    PIRi S05569.
    RefSeqi NP_414652.1. NC_000913.3.
    YP_488413.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P13016.
    SMRi P13016. Positions 1-179.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 511145.b0110.

    Proteomic databases

    PRIDEi P13016.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC73221 ; AAC73221 ; b0110 .
    BAB96679 ; BAB96679 ; BAB96679 .
    GeneIDi 12932901.
    948877.
    KEGGi ecj:Y75_p0107.
    eco:b0110.
    PATRICi 32115321. VBIEscCol129921_0112.

    Organism-specific databases

    EchoBASEi EB0039.
    EcoGenei EG10041. ampD.

    Phylogenomic databases

    eggNOGi COG3023.
    HOGENOMi HOG000255963.
    KOi K03806.
    OMAi GRPRCND.
    OrthoDBi EOG60GRR5.
    PhylomeDBi P13016.

    Enzyme and pathway databases

    BioCyci EcoCyc:EG10041-MONOMER.
    ECOL316407:JW0106-MONOMER.
    MetaCyc:EG10041-MONOMER.

    Miscellaneous databases

    PROi P13016.

    Gene expression databases

    Genevestigatori P13016.

    Family and domain databases

    Gene3Di 3.40.80.10. 1 hit.
    InterProi IPR002502. Amidase_domain.
    [Graphical view ]
    Pfami PF01510. Amidase_2. 1 hit.
    [Graphical view ]
    SMARTi SM00644. Ami_2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF55846. SSF55846. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Signalling proteins in enterobacterial AmpC beta-lactamase regulation."
      Lindquist S., Galleni M., Lindberg F., Normark S.
      Mol. Microbiol. 3:1091-1102(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12.
    2. "Regulation of enterobacterial cephalosporinase production: the role of a membrane-bound sensory transducer."
      Honore N., Nicolas M.H., Cole S.T.
      Mol. Microbiol. 3:1121-1130(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    5. "Escherichia coli contains a set of genes homologous to those involved in protein secretion, DNA uptake and the assembly of type-4 fimbriae in other bacteria."
      Whitchurch C.B., Mattick J.S.
      Gene 150:9-15(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-92.
      Strain: K12.

    Entry informationi

    Entry nameiAMPD_ECOLI
    AccessioniPrimary (citable) accession number: P13016
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1990
    Last sequence update: January 1, 1990
    Last modified: October 1, 2014
    This is version 114 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3