SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P13016

- AMPD_ECOLI

UniProt

P13016 - AMPD_ECOLI

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD
Gene
ampD, b0110, JW0106
Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Involved in both cell wall peptidoglycans recycling and beta-lactamase induction. Specifically cleaves the amide bond between the lactyl group of N-acetylmuramic acid and the alpha-amino group of the L-alanine in degradation products containing an anhydro N-acetylmuramyl moiety By similarity.

Catalytic activityi

Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.

Cofactori

Zinc; required for amidase activity By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi34 – 341Zinc; catalytic By similarity
Active sitei116 – 1161Proton acceptor By similarity
Metal bindingi154 – 1541Zinc; catalytic By similarity
Sitei162 – 1621Transition state stabilizer By similarity
Metal bindingi164 – 1641Zinc; catalytic By similarity

GO - Molecular functioni

  1. N-acetylmuramoyl-L-alanine amidase activity Source: EcoCyc
  2. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. peptidoglycan catabolic process Source: InterPro
  2. peptidoglycan turnover Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Cell wall biogenesis/degradation

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciEcoCyc:EG10041-MONOMER.
ECOL316407:JW0106-MONOMER.
MetaCyc:EG10041-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD (EC:3.5.1.28)
Alternative name(s):
N-acetylmuramoyl-L-alanine amidase
Gene namesi
Name:ampD
Ordered Locus Names:b0110, JW0106
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG10041. ampD.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 1831831,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD
PRO_0000164413Add
BLAST

Proteomic databases

PRIDEiP13016.

Expressioni

Inductioni

Inactivation of AmpD results in AmpR-dependent hyperinduction and AmpD affects beta-lactam susceptibility in the absence of cloned C.freundii amp genes suggesting a linkage between AmpD and peptidoglycan synthesis.

Gene expression databases

GenevestigatoriP13016.

Interactioni

Protein-protein interaction databases

STRINGi511145.b0110.

Structurei

3D structure databases

ProteinModelPortaliP13016.
SMRiP13016. Positions 1-179.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG3023.
HOGENOMiHOG000255963.
KOiK03806.
OMAiGRPRCND.
OrthoDBiEOG60GRR5.
PhylomeDBiP13016.

Family and domain databases

Gene3Di3.40.80.10. 1 hit.
InterProiIPR002502. Amidase_domain.
[Graphical view]
PfamiPF01510. Amidase_2. 1 hit.
[Graphical view]
SMARTiSM00644. Ami_2. 1 hit.
[Graphical view]
SUPFAMiSSF55846. SSF55846. 1 hit.

Sequencei

Sequence statusi: Complete.

P13016-1 [UniParc]FASTAAdd to Basket

« Hide

MLLEQGWLVG ARRVPSPHYD CRPDDETPTL LVVHNISLPP GEFGGPWIDA    50
LFTGTIDPQA HPFFAEIAHL RVSAHCLIRR DGEIVQYVPF DKRAWHAGVS 100
QYQGRERCND FSIGIELEGT DTLAYTDAQY QQLAAVTRAL IDCYPDIAKN 150
MTGHCDIAPD RKTDPGPAFD WARFRVLVSK ETT 183
Length:183
Mass (Da):20,536
Last modified:January 1, 1990 - v1
Checksum:iF62910F8F889043D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X15237 Genomic DNA. Translation: CAA33314.1.
U00096 Genomic DNA. Translation: AAC73221.1.
AP009048 Genomic DNA. Translation: BAB96679.1.
L28105 Genomic DNA. Translation: AAC36921.1.
PIRiS05569.
RefSeqiNP_414652.1. NC_000913.3.
YP_488413.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC73221; AAC73221; b0110.
BAB96679; BAB96679; BAB96679.
GeneIDi12932901.
948877.
KEGGiecj:Y75_p0107.
eco:b0110.
PATRICi32115321. VBIEscCol129921_0112.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X15237 Genomic DNA. Translation: CAA33314.1 .
U00096 Genomic DNA. Translation: AAC73221.1 .
AP009048 Genomic DNA. Translation: BAB96679.1 .
L28105 Genomic DNA. Translation: AAC36921.1 .
PIRi S05569.
RefSeqi NP_414652.1. NC_000913.3.
YP_488413.1. NC_007779.1.

3D structure databases

ProteinModelPortali P13016.
SMRi P13016. Positions 1-179.
ModBasei Search...

Protein-protein interaction databases

STRINGi 511145.b0110.

Proteomic databases

PRIDEi P13016.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC73221 ; AAC73221 ; b0110 .
BAB96679 ; BAB96679 ; BAB96679 .
GeneIDi 12932901.
948877.
KEGGi ecj:Y75_p0107.
eco:b0110.
PATRICi 32115321. VBIEscCol129921_0112.

Organism-specific databases

EchoBASEi EB0039.
EcoGenei EG10041. ampD.

Phylogenomic databases

eggNOGi COG3023.
HOGENOMi HOG000255963.
KOi K03806.
OMAi GRPRCND.
OrthoDBi EOG60GRR5.
PhylomeDBi P13016.

Enzyme and pathway databases

BioCyci EcoCyc:EG10041-MONOMER.
ECOL316407:JW0106-MONOMER.
MetaCyc:EG10041-MONOMER.

Miscellaneous databases

PROi P13016.

Gene expression databases

Genevestigatori P13016.

Family and domain databases

Gene3Di 3.40.80.10. 1 hit.
InterProi IPR002502. Amidase_domain.
[Graphical view ]
Pfami PF01510. Amidase_2. 1 hit.
[Graphical view ]
SMARTi SM00644. Ami_2. 1 hit.
[Graphical view ]
SUPFAMi SSF55846. SSF55846. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Signalling proteins in enterobacterial AmpC beta-lactamase regulation."
    Lindquist S., Galleni M., Lindberg F., Normark S.
    Mol. Microbiol. 3:1091-1102(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "Regulation of enterobacterial cephalosporinase production: the role of a membrane-bound sensory transducer."
    Honore N., Nicolas M.H., Cole S.T.
    Mol. Microbiol. 3:1121-1130(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Escherichia coli contains a set of genes homologous to those involved in protein secretion, DNA uptake and the assembly of type-4 fimbriae in other bacteria."
    Whitchurch C.B., Mattick J.S.
    Gene 150:9-15(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-92.
    Strain: K12.

Entry informationi

Entry nameiAMPD_ECOLI
AccessioniPrimary (citable) accession number: P13016
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: May 14, 2014
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi