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Protein

Malonyl-[acyl-carrier protein] O-methyltransferase

Gene

bioC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Converts the free carboxyl group of a malonyl-thioester to its methyl ester by transfer of a methyl group from S-adenosyl-L-methionine (SAM). It allows to synthesize pimeloyl-ACP via the fatty acid synthetic pathway. E.coli employs a methylation and demethylation strategy to allow elongation of a temporarily disguised malonate moiety to a pimelate moiety by the fatty acid synthetic enzymes.2 Publications

Catalytic activityi

S-adenosyl-L-methionine + malonyl-[acyl-carrier protein] = S-adenosyl-L-homocysteine + malonyl-[acyl-carrier protein] methyl ester.1 Publication

Pathwayi: biotin biosynthesis

This protein is involved in the pathway biotin biosynthesis, which is part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the pathway biotin biosynthesis and in Cofactor biosynthesis.

GO - Molecular functioni

  • carboxyl-O-methyltransferase activity Source: EcoCyc
  • malonyl-CoA methyltransferase activity Source: UniProtKB-EC

GO - Biological processi

  • biotin biosynthetic process Source: EcoCyc

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processBiotin biosynthesis
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciEcoCyc:EG10119-MONOMER.
MetaCyc:EG10119-MONOMER.
BRENDAi2.1.1.197. 2026.
UniPathwayiUPA00078.

Names & Taxonomyi

Protein namesi
Recommended name:
Malonyl-[acyl-carrier protein] O-methyltransferase (EC:2.1.1.197)
Short name:
Malonyl-ACP O-methyltransferase
Alternative name(s):
Biotin synthesis protein BioC
Gene namesi
Name:bioC
Ordered Locus Names:b0777, JW0760
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10119. bioC.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002044161 – 251Malonyl-[acyl-carrier protein] O-methyltransferaseAdd BLAST251

Proteomic databases

PaxDbiP12999.
PRIDEiP12999.

Interactioni

Protein-protein interaction databases

BioGridi4259952. 6 interactors.
IntActiP12999. 11 interactors.
STRINGi316385.ECDH10B_0845.

Structurei

3D structure databases

ProteinModelPortaliP12999.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the methyltransferase superfamily.Curated

Phylogenomic databases

eggNOGiENOG410626R. Bacteria.
COG0500. LUCA.
HOGENOMiHOG000265010.
InParanoidiP12999.
KOiK02169.
PhylomeDBiP12999.

Family and domain databases

HAMAPiMF_00835. BioC. 1 hit.
InterProiView protein in InterPro
IPR011814. BioC.
IPR013216. Methyltransf_11.
IPR029063. SAM-dependent_MTases.
PfamiView protein in Pfam
PF08241. Methyltransf_11. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR02072. BioC. 1 hit.

Sequencei

Sequence statusi: Complete.

P12999-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATVNKQAIA AAFGRAAAHY EQHADLQRQS ADALLAMLPQ RKYTHVLDAG
60 70 80 90 100
CGPGWMSRHW RERHAQVTAL DLSPPMLVQA RQKDAADHYL AGDIESLPLA
110 120 130 140 150
TATFDLAWSN LAVQWCGNLS TALRELYRVV RPKGVVAFTT LVQGSLPELH
160 170 180 190 200
QAWQAVDERP HANRFLPPDE IEQSLNGVHY QHHIQPITLW FDDALSAMRS
210 220 230 240 250
LKGIGATHLH EGRDPRILTR SQLQRLQLAW PQQQGRYPLT YHLFLGVIAR

E
Length:251
Mass (Da):28,276
Last modified:November 1, 1997 - v2
Checksum:iD07DA38FFE974E45
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti149L → R in AAA23517 (PubMed:3058702).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04423 Genomic DNA. Translation: AAA23517.1.
A11534 Unassigned DNA. Translation: CAA00966.1.
U00096 Genomic DNA. Translation: AAC73864.1.
AP009048 Genomic DNA. Translation: BAE76364.1.
PIRiA64814. BVECBC.
RefSeqiNP_415298.1. NC_000913.3.
WP_000246761.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73864; AAC73864; b0777.
BAE76364; BAE76364; BAE76364.
GeneIDi945388.
KEGGiecj:JW0760.
eco:b0777.
PATRICifig|1411691.4.peg.1501.

Similar proteinsi

Entry informationi

Entry nameiBIOC_ECOLI
AccessioniPrimary (citable) accession number: P12999
Secondary accession number(s): Q2MBJ2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: November 1, 1997
Last modified: September 27, 2017
This is version 123 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families