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Protein

Malonyl-[acyl-carrier protein] O-methyltransferase

Gene

bioC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Converts the free carboxyl group of a malonyl-thioester to its methyl ester by transfer of a methyl group from S-adenosyl-L-methionine (SAM). It allows to synthesize pimeloyl-ACP via the fatty acid synthetic pathway. E.coli employs a methylation and demethylation strategy to allow elongation of a temporarily disguised malonate moiety to a pimelate moiety by the fatty acid synthetic enzymes.2 Publications

Catalytic activityi

S-adenosyl-L-methionine + malonyl-[acyl-carrier protein] = S-adenosyl-L-homocysteine + malonyl-[acyl-carrier protein] methyl ester.1 Publication

Pathwayi: biotin biosynthesis

This protein is involved in the pathway biotin biosynthesis, which is part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the pathway biotin biosynthesis and in Cofactor biosynthesis.

GO - Molecular functioni

  • carboxyl-O-methyltransferase activity Source: EcoCyc
  • S-adenosylmethionine-dependent methyltransferase activity Source: GO_Central

GO - Biological processi

  • biotin biosynthetic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciEcoCyc:EG10119-MONOMER.
ECOL316407:JW0760-MONOMER.
MetaCyc:EG10119-MONOMER.
BRENDAi2.1.1.197. 2026.
UniPathwayiUPA00078.

Names & Taxonomyi

Protein namesi
Recommended name:
Malonyl-[acyl-carrier protein] O-methyltransferase (EC:2.1.1.197)
Short name:
Malonyl-ACP O-methyltransferase
Alternative name(s):
Biotin synthesis protein BioC
Gene namesi
Name:bioC
Ordered Locus Names:b0777, JW0760
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10119. bioC.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 251251Malonyl-[acyl-carrier protein] O-methyltransferasePRO_0000204416Add
BLAST

Proteomic databases

PaxDbiP12999.
PRIDEiP12999.

Interactioni

Protein-protein interaction databases

BioGridi4259952. 6 interactions.
IntActiP12999. 11 interactions.
STRINGi511145.b0777.

Structurei

3D structure databases

ProteinModelPortaliP12999.
SMRiP12999. Positions 33-140.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the methyltransferase superfamily.Curated

Phylogenomic databases

eggNOGiENOG410626R. Bacteria.
COG0500. LUCA.
HOGENOMiHOG000265010.
InParanoidiP12999.
KOiK02169.
OMAiSHFRLEK.
PhylomeDBiP12999.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_00835. BioC. 1 hit.
InterProiIPR011814. BioC.
IPR013216. Methyltransf_11.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF08241. Methyltransf_11. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR02072. BioC. 1 hit.

Sequencei

Sequence statusi: Complete.

P12999-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATVNKQAIA AAFGRAAAHY EQHADLQRQS ADALLAMLPQ RKYTHVLDAG
60 70 80 90 100
CGPGWMSRHW RERHAQVTAL DLSPPMLVQA RQKDAADHYL AGDIESLPLA
110 120 130 140 150
TATFDLAWSN LAVQWCGNLS TALRELYRVV RPKGVVAFTT LVQGSLPELH
160 170 180 190 200
QAWQAVDERP HANRFLPPDE IEQSLNGVHY QHHIQPITLW FDDALSAMRS
210 220 230 240 250
LKGIGATHLH EGRDPRILTR SQLQRLQLAW PQQQGRYPLT YHLFLGVIAR

E
Length:251
Mass (Da):28,276
Last modified:November 1, 1997 - v2
Checksum:iD07DA38FFE974E45
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti149 – 1491L → R in AAA23517 (PubMed:3058702).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04423 Genomic DNA. Translation: AAA23517.1.
A11534 Unassigned DNA. Translation: CAA00966.1.
U00096 Genomic DNA. Translation: AAC73864.1.
AP009048 Genomic DNA. Translation: BAE76364.1.
PIRiA64814. BVECBC.
RefSeqiNP_415298.1. NC_000913.3.
WP_000246761.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73864; AAC73864; b0777.
BAE76364; BAE76364; BAE76364.
GeneIDi945388.
KEGGiecj:JW0760.
eco:b0777.
PATRICi32116755. VBIEscCol129921_0803.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04423 Genomic DNA. Translation: AAA23517.1.
A11534 Unassigned DNA. Translation: CAA00966.1.
U00096 Genomic DNA. Translation: AAC73864.1.
AP009048 Genomic DNA. Translation: BAE76364.1.
PIRiA64814. BVECBC.
RefSeqiNP_415298.1. NC_000913.3.
WP_000246761.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP12999.
SMRiP12999. Positions 33-140.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259952. 6 interactions.
IntActiP12999. 11 interactions.
STRINGi511145.b0777.

Proteomic databases

PaxDbiP12999.
PRIDEiP12999.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73864; AAC73864; b0777.
BAE76364; BAE76364; BAE76364.
GeneIDi945388.
KEGGiecj:JW0760.
eco:b0777.
PATRICi32116755. VBIEscCol129921_0803.

Organism-specific databases

EchoBASEiEB0117.
EcoGeneiEG10119. bioC.

Phylogenomic databases

eggNOGiENOG410626R. Bacteria.
COG0500. LUCA.
HOGENOMiHOG000265010.
InParanoidiP12999.
KOiK02169.
OMAiSHFRLEK.
PhylomeDBiP12999.

Enzyme and pathway databases

UniPathwayiUPA00078.
BioCyciEcoCyc:EG10119-MONOMER.
ECOL316407:JW0760-MONOMER.
MetaCyc:EG10119-MONOMER.
BRENDAi2.1.1.197. 2026.

Miscellaneous databases

PROiP12999.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_00835. BioC. 1 hit.
InterProiIPR011814. BioC.
IPR013216. Methyltransf_11.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF08241. Methyltransf_11. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR02072. BioC. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiBIOC_ECOLI
AccessioniPrimary (citable) accession number: P12999
Secondary accession number(s): Q2MBJ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: November 1, 1997
Last modified: September 7, 2016
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.