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P12982

- PP12_DROME

UniProt

P12982 - PP12_DROME

Protein

Serine/threonine-protein phosphatase alpha-2 isoform

Gene

Pp1-87B

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 144 (01 Oct 2014)
      Sequence version 1 (01 Jan 1990)
      Previous versions | rss
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    Functioni

    Is essential for the regulation of mitotic chromosomal segregation as well as regulation of chromatin condensation during interphase.2 Publications

    Catalytic activityi

    [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

    Cofactori

    Binds 2 manganese ions per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi62 – 621Manganese 1By similarity
    Metal bindingi64 – 641Manganese 1By similarity
    Metal bindingi90 – 901Manganese 1By similarity
    Metal bindingi90 – 901Manganese 2By similarity
    Metal bindingi122 – 1221Manganese 2By similarity
    Active sitei123 – 1231Proton donorBy similarity
    Metal bindingi171 – 1711Manganese 2By similarity
    Metal bindingi246 – 2461Manganese 2By similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. myosin phosphatase activity Source: FlyBase
    3. protein binding Source: IntAct
    4. protein serine/threonine phosphatase activity Source: FlyBase

    GO - Biological processi

    1. adult locomotory behavior Source: FlyBase
    2. axon guidance Source: FlyBase
    3. chromosome condensation Source: FlyBase
    4. chromosome segregation Source: FlyBase
    5. learning or memory Source: FlyBase
    6. locomotion Source: FlyBase
    7. mitotic metaphase plate congression Source: FlyBase
    8. mitotic nuclear division Source: FlyBase
    9. mitotic spindle elongation Source: FlyBase
    10. nervous system development Source: FlyBase
    11. olfactory learning Source: FlyBase
    12. oogenesis Source: FlyBase
    13. phagocytosis Source: FlyBase
    14. protein dephosphorylation Source: FlyBase
    15. spindle assembly Source: FlyBase
    16. visual learning Source: FlyBase

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Ligandi

    Manganese, Metal-binding

    Enzyme and pathway databases

    SignaLinkiP12982.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein phosphatase alpha-2 isoform (EC:3.1.3.16)
    Gene namesi
    Name:Pp1-87B
    ORF Names:CG5650
    OrganismiDrosophila melanogaster (Fruit fly)
    Taxonomic identifieri7227 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000000803: Chromosome 3R

    Organism-specific databases

    FlyBaseiFBgn0004103. Pp1-87B.

    Subcellular locationi

    GO - Cellular componenti

    1. polytene chromosome Source: FlyBase
    2. protein phosphatase type 1 complex Source: FlyBase

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 302302Serine/threonine-protein phosphatase alpha-2 isoformPRO_0000058792Add
    BLAST

    Proteomic databases

    PaxDbiP12982.
    PRIDEiP12982.

    Expressioni

    Gene expression databases

    BgeeiP12982.

    Interactioni

    Subunit structurei

    Interacts with Nop17l.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Nop17lQ0E9G33EBI-152633,EBI-150380

    Protein-protein interaction databases

    BioGridi72126. 35 interactions.
    DIPiDIP-18465N.
    IntActiP12982. 41 interactions.
    MINTiMINT-741838.

    Structurei

    3D structure databases

    ProteinModelPortaliP12982.
    SMRiP12982. Positions 5-297.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the PPP phosphatase family. PP-1 subfamily.Curated

    Phylogenomic databases

    eggNOGiCOG0639.
    GeneTreeiENSGT00530000062911.
    InParanoidiP12982.
    KOiK06269.
    OMAiMGDVMNI.
    OrthoDBiEOG7TJ3K3.
    PhylomeDBiP12982.

    Family and domain databases

    Gene3Di3.60.21.10. 1 hit.
    InterProiIPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    [Graphical view]
    PfamiPF00149. Metallophos. 1 hit.
    [Graphical view]
    PRINTSiPR00114. STPHPHTASE.
    SMARTiSM00156. PP2Ac. 1 hit.
    [Graphical view]
    SUPFAMiSSF56300. SSF56300. 1 hit.
    PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P12982-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGDVMNIDSI ISRLLEVRGA RPGKNVQLSE GEIRGLCLKS REIFLSQPIL    50
    LELEAPLKIC GDIHGQYYDL LRLFEYGGFP PESNYLFLGD YVDRGKQSLE 100
    TICLLLAYKI KYSENFFLLR GNHECASINR IYGFYDECKR RYSIKLWKTF 150
    TDCFNCLPVA AIVDEKIFCC HGGLSPDLTS MEQIRRIMRP TDVPDQGLLC 200
    DLLWSDPDKD TMGWGENDRG VSFTFGAEVV AKFLQKHEFD LICRAHQVVE 250
    DGYEFFAKRM LVTLFSAPNY CGEFDNAGAM MSVDDTLMCS FQILKPADKR 300
    KK 302
    Length:302
    Mass (Da):34,542
    Last modified:January 1, 1990 - v1
    Checksum:i68FE054996C9A062
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X15583 mRNA. Translation: CAA33609.1.
    S47852 Genomic DNA. Translation: AAB23957.1.
    AE014297 Genomic DNA. Translation: AAF54810.1.
    AY061063 mRNA. Translation: AAL28611.1.
    PIRiS12960. PAFF1A.
    RefSeqiNP_524937.1. NM_080198.3.
    UniGeneiDm.1623.

    Genome annotation databases

    EnsemblMetazoaiFBtr0082595; FBpp0082067; FBgn0004103.
    GeneIDi49260.
    KEGGidme:Dmel_CG5650.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X15583 mRNA. Translation: CAA33609.1 .
    S47852 Genomic DNA. Translation: AAB23957.1 .
    AE014297 Genomic DNA. Translation: AAF54810.1 .
    AY061063 mRNA. Translation: AAL28611.1 .
    PIRi S12960. PAFF1A.
    RefSeqi NP_524937.1. NM_080198.3.
    UniGenei Dm.1623.

    3D structure databases

    ProteinModelPortali P12982.
    SMRi P12982. Positions 5-297.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 72126. 35 interactions.
    DIPi DIP-18465N.
    IntActi P12982. 41 interactions.
    MINTi MINT-741838.

    Proteomic databases

    PaxDbi P12982.
    PRIDEi P12982.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai FBtr0082595 ; FBpp0082067 ; FBgn0004103 .
    GeneIDi 49260.
    KEGGi dme:Dmel_CG5650.

    Organism-specific databases

    CTDi 49260.
    FlyBasei FBgn0004103. Pp1-87B.

    Phylogenomic databases

    eggNOGi COG0639.
    GeneTreei ENSGT00530000062911.
    InParanoidi P12982.
    KOi K06269.
    OMAi MGDVMNI.
    OrthoDBi EOG7TJ3K3.
    PhylomeDBi P12982.

    Enzyme and pathway databases

    SignaLinki P12982.

    Miscellaneous databases

    ChiTaRSi Pp1-87B. drosophila.
    GenomeRNAii 49260.
    NextBioi 839723.

    Gene expression databases

    Bgeei P12982.

    Family and domain databases

    Gene3Di 3.60.21.10. 1 hit.
    InterProi IPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    [Graphical view ]
    Pfami PF00149. Metallophos. 1 hit.
    [Graphical view ]
    PRINTSi PR00114. STPHPHTASE.
    SMARTi SM00156. PP2Ac. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56300. SSF56300. 1 hit.
    PROSITEi PS00125. SER_THR_PHOSPHATASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and chromosomal localization of Drosophila cDNA encoding the catalytic subunit of protein phosphatase 1 alpha. High conservation between mammalian and insect sequences."
      Dombradi V., Axton J.M., Glover D.M., Cohen P.T.W.
      Eur. J. Biochem. 183:603-610(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Head.
    2. "Protein phosphorylation is involved in the regulation of chromatin condensation during interphase."
      Dombradi V., Cohen P.T.W.
      FEBS Lett. 312:21-26(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
      Strain: Canton-S.
    3. "The genome sequence of Drosophila melanogaster."
      Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
      , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
      Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Berkeley.
    4. Cited for: GENOME REANNOTATION.
      Strain: Berkeley.
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Berkeley.
      Tissue: Embryo.
    6. "Protein phosphatase 1 activity in Drosophila mutants with abnormalities in mitosis and chromosome condensation."
      Dombradi V., Axton J.M., Barker H.M., Cohen P.T.W.
      FEBS Lett. 275:39-43(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "Towards a comprehensive analysis of the protein phosphatase 1 interactome in Drosophila."
      Bennett D., Lyulcheva E., Alphey L.
      J. Mol. Biol. 364:196-212(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH NOP17L.

    Entry informationi

    Entry nameiPP12_DROME
    AccessioniPrimary (citable) accession number: P12982
    Secondary accession number(s): Q4PIY5, Q9VG75
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1990
    Last sequence update: January 1, 1990
    Last modified: October 1, 2014
    This is version 144 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3