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P12978 (EBNA2_EBVB9) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 88. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Epstein-Barr nuclear antigen 2

Short name=EBNA-2
Short name=EBV nuclear antigen 2
Gene names
Name:EBNA2
ORF Names:BYRF1
OrganismEpstein-Barr virus (strain B95-8) (HHV-4) (Human herpesvirus 4) [Reference proteome]
Taxonomic identifier10377 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeGammaherpesvirinaeLymphocryptovirus
Virus hostHomo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length487 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Plays a key role in the activation of the host resting B-cell and stimulation of B-cell proliferation. Acts by up-regulating the expression of viral EBNA1-6, LMP1, LMP2A and LMP2B genes, as well as several host genes including CD21, CD23 and MYC. Activates transcription by acting as an adapter molecule that binds to cellular sequence-specific DNA-binding proteins such as host CBF1, SMARCB1 and SPI1. Once EBNA2 is near promoter sites, its acidic activating domain recruits basal and activation-associated transcription factors TFIIB, TAF40, TFIIH components ERCC2 and ERCC3, and CBP in order to promote transcription. Alternatively, EBNA2 can affect activities of cell cycle regulators and retard cell cycle progression at G2/M phase. It also induces chromosomal instability, by disrupting mitotic checkpoints, multi-nucleation and formation of micronuclei in infected cells. Ref.11

Subunit structure

Interacts with human SMARCB1/INI1, presumably generating an open chromatin conformation at the EBNA2-responsive target genes. Interacts with human WAPAL. Interacts with host CBF1; this interaction allows transcriptional activation by EBNA2. Interacts with host general transcription factors GTF2B, ERCC2 and ERCC3. Interacts (via PXLXP motif) with host ZMYND11/BS69 (via MYND-type zinc finger). Ref.5 Ref.6 Ref.7 Ref.8 Ref.9 Ref.10

Subcellular location

Host nucleus matrix. Note: Associated with the nuclear matrix. Ref.3

Post-translational modification

Phosphorylated. Ref.3

Sequence similarities

Belongs to the herpesviridae EBNA2 family.

Sequence caution

The sequence CAA24877.1 differs from that shown. Reason: Erroneous initiation.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

RBPJQ063302EBI-8052923,EBI-632552From a different organism.
ZMYND11Q153262EBI-8052923,EBI-2623509From a different organism.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 487487Epstein-Barr nuclear antigen 2
PRO_0000116176

Regions

Repeat345 – 34621
Repeat347 – 34822
Repeat349 – 35023
Repeat351 – 35224
Repeat353 – 35425
Repeat355 – 35626
Repeat357 – 35827
Region1 – 210210SMARCB1/INI1 binding
Region345 – 358146.5 X 2 AA tandem repeats of R-G
Motif383 – 3875PXLXP motif, interaction with host ZMYND11
Motif437 – 4415PXLXP motif, interaction with host ZMYND11
Compositional bias59 – 10042Poly-Pro

Experimental info

Mutagenesis3851L → A: Complete loss of interaction with host ZMYND11. Ref.9
Mutagenesis4391L → A: Complete loss of interaction with host ZMYND11. Ref.9
Sequence conflict881P → PPPP in AAA45903. Ref.1

Secondary structure

... 487
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P12978 [UniParc].

Last modified October 1, 1989. Version 1.
Checksum: DEF40D7F8ED61D1A

FASTA48752,545
        10         20         30         40         50         60 
MPTFYLALHG GQTYHLIVDT DSLGNPSLSV IPSNPYQEQL SDTPLIPLTI FVGENTGVPP 

        70         80         90        100        110        120 
PLPPPPPPPP PPPPPPPPPP PPPPPPPPSP PPPPPPPPPP QRRDAWTQEP SPLDRDPLGY 

       130        140        150        160        170        180 
DVGHGPLASA MRMLWMANYI VRQSRGDRGL ILPQGPQTAP QARLVQPHVP PLRPTAPTIL 

       190        200        210        220        230        240 
SPLSQPRLTP PQPLMMPPRP TPPTPLPPAT LTVPPRPTRP TTLPPTPLLT VLQRPTELQP 

       250        260        270        280        290        300 
TPSPPRMHLP VLHVPDQSMH PLTHQSTPND PDSPEPRSPT VFYNIPPMPL PPSQLPPPAA 

       310        320        330        340        350        360 
PAQPPPGVIN DQQLHHLPSG PPWWPPICDP PQPSKTQGQS RGQSRGRGRG RGRGRGKGKS 

       370        380        390        400        410        420 
RDKQRKPGGP WRPEPNTSSP SMPELSPVLG LHQGQGAGDS PTPGPSNAAP VCRNSHTATP 

       430        440        450        460        470        480 
NVSPIHEPES HNSPEAPILF PDDWYPPSID PADLDESWDY IFETTESPSS DEDYVEGPSK 


RPRPSIQ 

« Hide

References

« Hide 'large scale' references
[1]"U2 region of Epstein-Barr virus DNA may encode Epstein-Barr nuclear antigen 2."
Dambaugh T., Hennessy K., Chamnankit L., Kieff E.
Proc. Natl. Acad. Sci. U.S.A. 81:7632-7636(1984) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"DNA sequence and expression of the B95-8 Epstein-Barr virus genome."
Baer R., Bankier A.T., Biggin M.D., Deininger P.L., Farrell P.J., Gibson T.J., Hatfull G., Hudson G.S., Satchwell S.C., Seguin C., Tuffnell P.S., Barrell B.G.
Nature 310:207-211(1984) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"Subnuclear localization and phosphorylation of Epstein-Barr virus latent infection nuclear proteins."
Petti L., Sample C., Kieff E.
Virology 176:563-574(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, PHOSPHORYLATION.
[4]"Epstein-Barr virus nuclear protein 2 mutations define essential domains for transformation and transactivation."
Cohen J.I., Wang F., Kieff E.
J. Virol. 65:2545-2554(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: DOMAINS.
[5]"Mediation of Epstein-Barr virus EBNA2 transactivation by recombination signal-binding protein J kappa."
Henkel T., Ling P.D., Hayward S.D., Peterson M.G.
Science 265:92-95(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH HUMAN CBF1.
[6]"The 62- and 80-kDa subunits of transcription factor IIH mediate the interaction with Epstein-Barr virus nuclear protein 2."
Tong X., Drapkin R., Reinberg D., Kieff E.
Proc. Natl. Acad. Sci. U.S.A. 92:3259-3263(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH HUMAN ERCC2 AND ERCC3.
[7]"The Epstein-Barr virus nuclear protein 2 acidic domain can interact with TFIIB, TAF40, and RPA70 but not with TATA-binding protein."
Tong X., Wang F., Thut C.J., Kieff E.
J. Virol. 69:585-588(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH HUMAN GTF2B.
[8]"Epstein-Barr virus nuclear protein 2 (EBNA2) binds to a component of the human SNF-SWI complex, hSNF5/Ini1."
Wu D.Y., Kalpana G.V., Goff S.P., Schubach W.H.
J. Virol. 70:6020-6028(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH HUMAN SMARCB1/INI1.
[9]"The conserved Mynd domain of BS69 binds cellular and oncoviral proteins through a common PXLXP motif."
Ansieau S., Leutz A.
J. Biol. Chem. 277:4906-4910(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH HUMAN ZMYND11/BS69, MUTAGENESIS OF LEU-385 AND LEU-439.
[10]"Identification and cloning of a novel chromatin-associated protein partner of Epstein-Barr nuclear protein 2."
Kwiatkowski B.A., Ragoczy T., Ehly J., Schubach W.H.
Exp. Cell Res. 300:223-233(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH HUMAN WAPAL.
[11]"Epstein-Barr virus nuclear antigen 2 disrupts mitotic checkpoint and causes chromosomal instability."
Pan S.H., Tai C.C., Lin C.S., Hsu W.B., Chou S.F., Lai C.C., Chen J.Y., Tien H.F., Lee F.Y., Wang W.B.
Carcinogenesis 30:366-375(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
K03333 Genomic DNA. Translation: AAA45903.1.
V01555 Genomic DNA. Translation: CAA24877.1. Different initiation.
AJ507799 Genomic DNA. Translation: CAD53395.1.
PIRS42442.
RefSeqYP_401644.1. NC_007605.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2MKRNMR-B453-465[»]
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP12978. 4 interactions.
MINTMINT-2576568.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID3783761.

Family and domain databases

ProtoNetSearch...

Entry information

Entry nameEBNA2_EBVB9
AccessionPrimary (citable) accession number: P12978
Secondary accession number(s): Q69023, Q777H1
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 1, 1989
Last modified: June 11, 2014
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references