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P12978

- EBNA2_EBVB9

UniProt

P12978 - EBNA2_EBVB9

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Protein
Epstein-Barr nuclear antigen 2
Gene
EBNA2, BYRF1
Organism
Epstein-Barr virus (strain B95-8) (HHV-4) (Human herpesvirus 4)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Plays a key role in the activation of the host resting B-cell and stimulation of B-cell proliferation. Acts by up-regulating the expression of viral EBNA1-6, LMP1, LMP2A and LMP2B genes, as well as several host genes including CD21, CD23 and MYC. Activates transcription by acting as an adapter molecule that binds to cellular sequence-specific DNA-binding proteins such as host CBF1, SMARCB1 and SPI1. Once EBNA2 is near promoter sites, its acidic activating domain recruits basal and activation-associated transcription factors TFIIB, TAF40, TFIIH components ERCC2 and ERCC3, and CBP in order to promote transcription. Alternatively, EBNA2 can affect activities of cell cycle regulators and retard cell cycle progression at G2/M phase. It also induces chromosomal instability, by disrupting mitotic checkpoints, multi-nucleation and formation of micronuclei in infected cells.1 Publication

GO - Molecular functioni

  1. protein binding Source: IntAct

GO - Biological processi

  1. modulation by virus of host PP1 activity Source: UniProtKB-KW
  2. modulation by virus of host cell cycle Source: UniProtKB-KW
  3. positive regulation of transcription, DNA-templated Source: BHF-UCL
  4. suppression by virus of host type I interferon-mediated signaling pathway Source: UniProtKB-KW
  5. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host interferon signaling pathway by virus, Modulation of host cell cycle by virus, Modulation of host PP1 activity by virus, Transcription, Transcription regulation, Viral immunoevasion

Names & Taxonomyi

Protein namesi
Recommended name:
Epstein-Barr nuclear antigen 2
Short name:
EBNA-2
Short name:
EBV nuclear antigen 2
Gene namesi
Name:EBNA2
ORF Names:BYRF1
OrganismiEpstein-Barr virus (strain B95-8) (HHV-4) (Human herpesvirus 4)
Taxonomic identifieri10377 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeGammaherpesvirinaeLymphocryptovirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
ProteomesiUP000007640: Genome

Subcellular locationi

Host nucleus matrix
Note: Associated with the nuclear matrix.1 Publication

GO - Cellular componenti

  1. host cell nuclear matrix Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Host nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi385 – 3851L → A: Complete loss of interaction with host ZMYND11. 1 Publication
Mutagenesisi439 – 4391L → A: Complete loss of interaction with host ZMYND11. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 487487Epstein-Barr nuclear antigen 2
PRO_0000116176Add
BLAST

Post-translational modificationi

Phosphorylated.1 Publication

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Interacts with human SMARCB1/INI1, presumably generating an open chromatin conformation at the EBNA2-responsive target genes. Interacts with human WAPAL. Interacts with host CBF1; this interaction allows transcriptional activation by EBNA2. Interacts with host general transcription factors GTF2B, ERCC2 and ERCC3. Interacts (via PXLXP motif) with host ZMYND11/BS69 (via MYND-type zinc finger).6 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
RBPJQ063302EBI-8052923,EBI-632552From a different organism.
ZMYND11Q153262EBI-8052923,EBI-2623509From a different organism.

Protein-protein interaction databases

IntActiP12978. 4 interactions.
MINTiMINT-2576568.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi455 – 4639

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2MKRNMR-B453-465[»]

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati345 – 34621
Repeati347 – 34822
Repeati349 – 35023
Repeati351 – 35224
Repeati353 – 35425
Repeati355 – 35626
Repeati357 – 35827

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 210210SMARCB1/INI1 binding
Add
BLAST
Regioni345 – 358146.5 X 2 AA tandem repeats of R-G
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi383 – 3875PXLXP motif, interaction with host ZMYND11
Motifi437 – 4415PXLXP motif, interaction with host ZMYND11

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi59 – 10042Poly-Pro
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Repeat

Sequencei

Sequence statusi: Complete.

P12978-1 [UniParc]FASTAAdd to Basket

« Hide

MPTFYLALHG GQTYHLIVDT DSLGNPSLSV IPSNPYQEQL SDTPLIPLTI    50
FVGENTGVPP PLPPPPPPPP PPPPPPPPPP PPPPPPPPSP PPPPPPPPPP 100
QRRDAWTQEP SPLDRDPLGY DVGHGPLASA MRMLWMANYI VRQSRGDRGL 150
ILPQGPQTAP QARLVQPHVP PLRPTAPTIL SPLSQPRLTP PQPLMMPPRP 200
TPPTPLPPAT LTVPPRPTRP TTLPPTPLLT VLQRPTELQP TPSPPRMHLP 250
VLHVPDQSMH PLTHQSTPND PDSPEPRSPT VFYNIPPMPL PPSQLPPPAA 300
PAQPPPGVIN DQQLHHLPSG PPWWPPICDP PQPSKTQGQS RGQSRGRGRG 350
RGRGRGKGKS RDKQRKPGGP WRPEPNTSSP SMPELSPVLG LHQGQGAGDS 400
PTPGPSNAAP VCRNSHTATP NVSPIHEPES HNSPEAPILF PDDWYPPSID 450
PADLDESWDY IFETTESPSS DEDYVEGPSK RPRPSIQ 487
Length:487
Mass (Da):52,545
Last modified:October 1, 1989 - v1
Checksum:iDEF40D7F8ED61D1A
GO

Sequence cautioni

The sequence CAA24877.1 differs from that shown. Reason: Erroneous initiation.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti88 – 881P → PPPP in AAA45903. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
K03333 Genomic DNA. Translation: AAA45903.1.
V01555 Genomic DNA. Translation: CAA24877.1. Different initiation.
AJ507799 Genomic DNA. Translation: CAD53395.1.
PIRiS42442.
RefSeqiYP_401644.1. NC_007605.1.

Genome annotation databases

GeneIDi3783761.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
K03333 Genomic DNA. Translation: AAA45903.1 .
V01555 Genomic DNA. Translation: CAA24877.1 . Different initiation.
AJ507799 Genomic DNA. Translation: CAD53395.1 .
PIRi S42442.
RefSeqi YP_401644.1. NC_007605.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2MKR NMR - B 453-465 [» ]
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P12978. 4 interactions.
MINTi MINT-2576568.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 3783761.

Family and domain databases

ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "U2 region of Epstein-Barr virus DNA may encode Epstein-Barr nuclear antigen 2."
    Dambaugh T., Hennessy K., Chamnankit L., Kieff E.
    Proc. Natl. Acad. Sci. U.S.A. 81:7632-7636(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "Subnuclear localization and phosphorylation of Epstein-Barr virus latent infection nuclear proteins."
    Petti L., Sample C., Kieff E.
    Virology 176:563-574(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, PHOSPHORYLATION.
  4. "Epstein-Barr virus nuclear protein 2 mutations define essential domains for transformation and transactivation."
    Cohen J.I., Wang F., Kieff E.
    J. Virol. 65:2545-2554(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: DOMAINS.
  5. "Mediation of Epstein-Barr virus EBNA2 transactivation by recombination signal-binding protein J kappa."
    Henkel T., Ling P.D., Hayward S.D., Peterson M.G.
    Science 265:92-95(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH HUMAN CBF1.
  6. "The 62- and 80-kDa subunits of transcription factor IIH mediate the interaction with Epstein-Barr virus nuclear protein 2."
    Tong X., Drapkin R., Reinberg D., Kieff E.
    Proc. Natl. Acad. Sci. U.S.A. 92:3259-3263(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH HUMAN ERCC2 AND ERCC3.
  7. "The Epstein-Barr virus nuclear protein 2 acidic domain can interact with TFIIB, TAF40, and RPA70 but not with TATA-binding protein."
    Tong X., Wang F., Thut C.J., Kieff E.
    J. Virol. 69:585-588(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH HUMAN GTF2B.
  8. "Epstein-Barr virus nuclear protein 2 (EBNA2) binds to a component of the human SNF-SWI complex, hSNF5/Ini1."
    Wu D.Y., Kalpana G.V., Goff S.P., Schubach W.H.
    J. Virol. 70:6020-6028(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH HUMAN SMARCB1/INI1.
  9. "The conserved Mynd domain of BS69 binds cellular and oncoviral proteins through a common PXLXP motif."
    Ansieau S., Leutz A.
    J. Biol. Chem. 277:4906-4910(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH HUMAN ZMYND11/BS69, MUTAGENESIS OF LEU-385 AND LEU-439.
  10. "Identification and cloning of a novel chromatin-associated protein partner of Epstein-Barr nuclear protein 2."
    Kwiatkowski B.A., Ragoczy T., Ehly J., Schubach W.H.
    Exp. Cell Res. 300:223-233(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH HUMAN WAPAL.
  11. "Epstein-Barr virus nuclear antigen 2 disrupts mitotic checkpoint and causes chromosomal instability."
    Pan S.H., Tai C.C., Lin C.S., Hsu W.B., Chou S.F., Lai C.C., Chen J.Y., Tien H.F., Lee F.Y., Wang W.B.
    Carcinogenesis 30:366-375(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiEBNA2_EBVB9
AccessioniPrimary (citable) accession number: P12978
Secondary accession number(s): Q69023, Q777H1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 1, 1989
Last modified: June 11, 2014
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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