Reviewed, UniProtKB/Swiss-Prot P12973 (MCRG_METFE)

Names and origin

Protein names

Recommended name:
    Methyl-coenzyme M reductase subunit gamma
    EC=2.8.4.1
Alternative name(s):
    Coenzyme-B sulfoethylthiotransferase gamma

Gene names

Name:

mcrG

Organism

Methanothermus fervidus

Taxonomic identifier

2180 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanothermaceae › Methanothermus

Protein attributes

Sequence length	249 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Reduction of methyl-coenzyme M (2-(methylthio) ethanesulfonic acid) with 7-mercaptoheptanoylthreonine phosphate to methane and an heterodisulfide.
Catalytic activity	2-(methylthio)ethanesulfonate (methyl-CoM) + N-(7-mercaptoheptanoyl)threonine 3-O-phosphate (coenzyme B) = CoM-S-S-CoB + methane.
Cofactor	Binds 2 coenzyme F430 noncovalently per hexamer. Coenzyme F430 is a yellow nickel porphinoid By similarity.
Pathway	One-carbon metabolism; methyl-coenzyme M reduction; methane from methyl-coenzyme M: step 1/1.
Subunit structure	Hexamer of two alpha, two beta, and two gamma chains.

Ontologies

Keywords
Biological process	Methanogenesis
Molecular function	Transferase
Gene Ontology (GO)
Biological process	methanogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	coenzyme-B sulfoethylthiotransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 249	249	Methyl-coenzyme M reductase subunit gamma		PRO_0000147474

Sequences

	Sequence		Length	Mass (Da)
	P12973-1 [UniParc]. Last modified October 1, 1989. Version 1. Checksum: 9EBC01D15018300F	FASTA	249	28,856
	10 20 30 40 50 60 MAQFYPGSTK IAENRRKFMN PDAELEKLRE ISDEDVVRIL GHRAPGEEYP SVHPPLEELD 70 80 90 100 110 120 EPEDPIKDIV EPTEGAKAGD RVRYVQFTDS VYFAPAQPYI RSRAYLWRYR GADAGTLSGR 130 140 150 160 170 180 QIIEARERDV EKIAKELIET EFFDPARTGI RGKSVHGHSL RLDENGMMFD MLRRQVYDEE 190 200 210 220 230 240 TGRVKMVKNQ IGDEFDEPID LGEPLDEETL KEKTTIYRID NIPYREDKDL LEIVQRIHQL RSEAGFSPE

References

[1]

"Structure and comparative analysis of the genes encoding component C of methyl coenzyme M reductase in the extremely thermophilic archaebacterium Methanothermus fervidus." Weil C.F., Cram D.S., Sherf B.A., Reeve J.N. J. Bacteriol. 170:4718-4726(1988) [PubMed: 3170483] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	J03375 Genomic DNA. Translation: AAA72196.1.
3D structure databases
HSSP	HSSP built from PDB template 1HBN based on UniProtKB P11562.
SMR	P12973. Positions 2-247.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.8.4.1. 7358.
Family and domain databases
InterPro	IPR003178. Me_CoM_Rdtase_gsu. [Graphical view]
Gene3D	G3DSA:3.90.320.20. MCR_gamma. 1 hit.
Pfam	PF02240. MCR_gamma. 1 hit. [Graphical view]
PIRSF	PIRSF000264. Meth_CoM_rd_gama. 1 hit.
ProDom	PD005845. MCR_gamma. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR03259. met_CoM_red_gam. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MCRG_METFE

Accession

Primary (citable) accession number: P12973

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	October 1, 1989
Last modified:	September 22, 2009
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)