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P12972 (MCRB_METFE) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Methyl-coenzyme M reductase subunit beta

EC=2.8.4.1
Alternative name(s):
Coenzyme-B sulfoethylthiotransferase beta
Gene names
Name:mcrB
OrganismMethanothermus fervidus
Taxonomic identifier2180 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanobacteriaMethanobacterialesMethanothermaceaeMethanothermus

Protein attributes

Sequence length438 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Reduction of methyl-coenzyme M (2-(methylthio) ethanesulfonic acid) with 7-mercaptoheptanoylthreonine phosphate to methane and a heterodisulfide.

Catalytic activity

Methyl-CoM + CoB = CoM-S-S-CoB + methane.

Cofactor

Binds 2 coenzyme F430 noncovalently per hexamer. Coenzyme F430 is a yellow nickel porphinoid By similarity.

Pathway

One-carbon metabolism; methyl-coenzyme M reduction; methane from methyl-coenzyme M: step 1/1.

Subunit structure

Hexamer of two alpha, two beta, and two gamma chains.

Ontologies

Keywords
   Biological processMethanogenesis
   Molecular functionTransferase
Gene Ontology (GO)
   Biological_processmethanogenesis

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functioncoenzyme-B sulfoethylthiotransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 438438Methyl-coenzyme M reductase subunit beta
PRO_0000147463

Sequences

Sequence LengthMass (Da)Tools
P12972 [UniParc].

Last modified October 1, 1989. Version 1.
Checksum: 76E0EE0DCB8CEC75

FASTA43847,034
        10         20         30         40         50         60 
MPKYEDKVDL YDDRGNLVEE QVPIEALSPL RNTAIKKIIH DIKRTVAVNL EGIENALRSA 

        70         80         90        100        110        120 
KVGGSGCHIP GRELDVDVID NAEAIAEKAK EMIQVEEGDD TVVELLHDGK RALVKVPSSR 

       130        140        150        160        170        180 
LESAAEYSVA PLVTASAFIQ SIIDVCDISI YDANMVKAAV LGRYPQSVEY VGGNIATMLD 

       190        200        210        220        230        240 
IPQKLEGPGY ALRNILVNHI VAATLKNTLQ AVALSSILEH TAMFEMGDAV GKFERLHLLG 

       250        260        270        280        290        300 
LAYQGLNADN LLYDLVKANG KDGTVGSVVE DVVERAKEDG VIKVEKELNG YKVYGTDDLA 

       310        320        330        340        350        360 
LWNAYAAAGL VAATIVNQGA ARAAQGVSST ILYDNDIIEF ERGLPGVDFG RAEGTAVGFS 

       370        380        390        400        410        420 
FFSHSIYGGG GPGIFNGNHI VTRHSKGFAI PCVAAAMALD AGTQMFSPEL TSGLIKDVFS 

       430 
KVDEFREPLK YVVELQPK 

« Hide

References

[1]"Structure and comparative analysis of the genes encoding component C of methyl coenzyme M reductase in the extremely thermophilic archaebacterium Methanothermus fervidus."
Weil C.F., Cram D.S., Sherf B.A., Reeve J.N.
J. Bacteriol. 170:4718-4726(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
J03375 Genomic DNA. Translation: AAA72193.1.

3D structure databases

ProteinModelPortalP12972.
SMRP12972. Positions 3-434.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00646; UER00699.

Family and domain databases

Gene3D1.20.840.10. 2 hits.
3.30.70.470. 1 hit.
InterProIPR022681. MCR_a/b_chain_a-bundle.
IPR008924. Me_CoM_Rdtase_asu/bsu_C.
IPR015823. Me_CoM_Rdtase_asu_N_sub2.
IPR003179. Me_CoM_Rdtase_bsu.
IPR022679. Me_CoM_Rdtase_bsu_C.
IPR022680. Me_CoM_Rdtase_bsu_N.
IPR009024. Me_CoM_Rdtase_Fd-like_fold.
[Graphical view]
PfamPF02241. MCR_beta. 1 hit.
PF02783. MCR_beta_N. 1 hit.
[Graphical view]
PIRSFPIRSF000263. Meth_CoM_rd_beta. 1 hit.
SUPFAMSSF48081. SSF48081. 1 hit.
SSF55088. SSF55088. 1 hit.
TIGRFAMsTIGR03257. met_CoM_red_bet. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMCRB_METFE
AccessionPrimary (citable) accession number: P12972
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 1, 1989
Last modified: October 16, 2013
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways