P12972 (MCRB_METFE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methyl-coenzyme M reductase subunit beta
EC=2.8.4.1

Alternative name(s):
Coenzyme-B sulfoethylthiotransferase beta

Gene names

Name:

mcrB

Organism

Methanothermus fervidus

Taxonomic identifier

2180 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanobacteria › Methanobacteriales › Methanothermaceae › Methanothermus

Protein attributes

Sequence length	438 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Reduction of methyl-coenzyme M (2-(methylthio) ethanesulfonic acid) with 7-mercaptoheptanoylthreonine phosphate to methane and an heterodisulfide.
Catalytic activity	Methyl-CoM + CoB = CoM-S-S-CoB + methane.
Cofactor	Binds 2 coenzyme F430 noncovalently per hexamer. Coenzyme F430 is a yellow nickel porphinoid By similarity.
Pathway	One-carbon metabolism; methyl-coenzyme M reduction; methane from methyl-coenzyme M: step 1/1.
Subunit structure	Hexamer of two alpha, two beta, and two gamma chains.

Ontologies

Keywords
Biological process	Methanogenesis
Molecular function	Transferase
Gene Ontology (GO)
Biological process	methanogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	coenzyme-B sulfoethylthiotransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 438	438	Methyl-coenzyme M reductase subunit beta		PRO_0000147463

Sequences

	Sequence		Length	Mass (Da)
	P12972 [UniParc]. Last modified October 1, 1989. Version 1. Checksum: 76E0EE0DCB8CEC75	FASTA	438	47,034
	10 20 30 40 50 60 MPKYEDKVDL YDDRGNLVEE QVPIEALSPL RNTAIKKIIH DIKRTVAVNL EGIENALRSA 70 80 90 100 110 120 KVGGSGCHIP GRELDVDVID NAEAIAEKAK EMIQVEEGDD TVVELLHDGK RALVKVPSSR 130 140 150 160 170 180 LESAAEYSVA PLVTASAFIQ SIIDVCDISI YDANMVKAAV LGRYPQSVEY VGGNIATMLD 190 200 210 220 230 240 IPQKLEGPGY ALRNILVNHI VAATLKNTLQ AVALSSILEH TAMFEMGDAV GKFERLHLLG 250 260 270 280 290 300 LAYQGLNADN LLYDLVKANG KDGTVGSVVE DVVERAKEDG VIKVEKELNG YKVYGTDDLA 310 320 330 340 350 360 LWNAYAAAGL VAATIVNQGA ARAAQGVSST ILYDNDIIEF ERGLPGVDFG RAEGTAVGFS 370 380 390 400 410 420 FFSHSIYGGG GPGIFNGNHI VTRHSKGFAI PCVAAAMALD AGTQMFSPEL TSGLIKDVFS 430 KVDEFREPLK YVVELQPK

References

[1]	"Structure and comparative analysis of the genes encoding component C of methyl coenzyme M reductase in the extremely thermophilic archaebacterium Methanothermus fervidus." Weil C.F., Cram D.S., Sherf B.A., Reeve J.N. J. Bacteriol. 170:4718-4726(1988) [PubMed: 3170483] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	J03375 Genomic DNA. Translation: AAA72193.1.
3D structure databases
ProteinModelPortal	P12972.
SMR	P12972. Positions 3-434.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR022681. MCR_a/b_chain_a-bundle. IPR008924. Me_CoM_Rdtase_asu/bsu_C. IPR015823. Me_CoM_Rdtase_asu_N_sub2. IPR003179. Me_CoM_Rdtase_bsu. IPR022679. Me_CoM_Rdtase_bsu_C. IPR022680. Me_CoM_Rdtase_bsu_N. IPR009024. Me_CoM_Rdtase_Fd-like_fold. [Graphical view]
Gene3D	G3DSA:1.20.840.10. MCR_a/b_chain_a-bundle. 2 hits. G3DSA:3.30.70.470. Me_CoM_Rdtase_asu_N_sub2. 1 hit.
Pfam	PF02241. MCR_beta. 1 hit. PF02783. MCR_beta_N. 1 hit. [Graphical view]
PIRSF	PIRSF000263. Meth_CoM_rd_beta. 1 hit.
SUPFAM	SSF48081. MCR_alpha_beta_C. 1 hit. SSF55088. MCR_fer_like. 1 hit.
TIGRFAMs	TIGR03257. Met_CoM_red_bet. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MCRB_METFE

Accession

Primary (citable) accession number: P12972

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	October 1, 1989
Last modified:	January 25, 2012
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program