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Protein

Islet amyloid polypeptide

Gene

Iapp

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Selectively inhibits insulin-stimulated glucose utilization and glycogen deposition in muscle, while not affecting adipocyte glucose metabolism.By similarity

GO - Molecular functioni

  • hormone activity Source: RGD

GO - Biological processi

  • eating behavior Source: RGD
  • glucose metabolic process Source: RGD
  • negative regulation of bone resorption Source: Ensembl
  • negative regulation of cell differentiation Source: Ensembl
  • sensory perception of pain Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Hormone

Enzyme and pathway databases

ReactomeiR-RNO-418555. G alpha (s) signalling events.
R-RNO-419812. Calcitonin-like ligand receptors.

Names & Taxonomyi

Protein namesi
Recommended name:
Islet amyloid polypeptide
Alternative name(s):
Amylin
Diabetes-associated peptide
Short name:
DAP
Gene namesi
Name:Iapp
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 4

Organism-specific databases

RGDi2854. Iapp.

Subcellular locationi

GO - Cellular componenti

  • extracellular space Source: Ensembl
  • neuronal cell body Source: Ensembl
  • secretory granule Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Amyloid, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Sequence analysisAdd
BLAST
Propeptidei24 – 3512PRO_0000004121Add
BLAST
Peptidei38 – 7437Islet amyloid polypeptidePRO_0000004122Add
BLAST
Propeptidei78 – 9316PRO_0000004123Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi39 ↔ 441 Publication
Modified residuei74 – 741Tyrosine amide1 Publication

Keywords - PTMi

Amidation, Cleavage on pair of basic residues, Disulfide bond

Proteomic databases

PaxDbiP12969.

Expressioni

Tissue specificityi

Abundant in the islets of Langerhans but is not present in the brain or seven other tissues examined.

Gene expression databases

GenevisibleiP12969. RN.

Interactioni

Subunit structurei

Interacts with IDE and INS.By similarity

GO - Molecular functioni

  • hormone activity Source: RGD

Protein-protein interaction databases

BioGridi246637. 1 interaction.
STRINGi10116.ENSRNOP00000016614.

Structurei

Secondary structure

1
93
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi42 – 5413Combined sources
Helixi57 – 604Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2KJ7NMR-A38-74[»]
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP12969.

Family & Domainsi

Domaini

The mature protein is largely unstructured in the absence of a cognate ligand, but contrary to the human protein, it does not easily form fibrillar aggregates.1 Publication

Sequence similaritiesi

Belongs to the calcitonin family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410J0NB. Eukaryota.
ENOG410Z7V7. LUCA.
GeneTreeiENSGT00510000048671.
HOGENOMiHOG000038203.
HOVERGENiHBG096065.
InParanoidiP12969.
KOiK08039.
OMAiSHQMEKR.
OrthoDBiEOG7TMZVK.
PhylomeDBiP12969.
TreeFamiTF330783.

Family and domain databases

InterProiIPR021117. Calcitonin-like.
IPR021116. Calcitonin/adrenomedullin.
IPR018360. Calcitonin_CS.
IPR001693. Calcitonin_peptide-like.
IPR000443. Pro-islet_amyloid_polypep.
[Graphical view]
PANTHERiPTHR10505. PTHR10505. 1 hit.
PfamiPF00214. Calc_CGRP_IAPP. 1 hit.
[Graphical view]
PRINTSiPR00818. ISLETAMYLOID.
SMARTiSM00113. CALCITONIN. 1 hit.
[Graphical view]
PROSITEiPS00258. CALCITONIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P12969-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRCISRLPAV LLILSVALGH LRATPVGSGT NPQVDKRKCN TATCATQRLA
60 70 80 90
NFLVRSSNNL GPVLPPTNVG SNTYGKRNVA EDPNRESLDF LLL
Length:93
Mass (Da):10,015
Last modified:October 1, 1989 - v1
Checksum:i5A76C92E624DA962
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M25390 mRNA. Translation: AAA41359.1.
J04544 mRNA. Translation: AAA40730.1.
X52820, X52821 Genomic DNA. Translation: CAA37003.1.
PIRiS13566. TCRTIA.
RefSeqiNP_036718.1. NM_012586.2.
XP_006237676.1. XM_006237614.2.
UniGeneiRn.11394.

Genome annotation databases

EnsembliENSRNOT00000016614; ENSRNOP00000016614; ENSRNOG00000012417.
GeneIDi24476.
KEGGirno:24476.
UCSCiRGD:2854. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M25390 mRNA. Translation: AAA41359.1.
J04544 mRNA. Translation: AAA40730.1.
X52820, X52821 Genomic DNA. Translation: CAA37003.1.
PIRiS13566. TCRTIA.
RefSeqiNP_036718.1. NM_012586.2.
XP_006237676.1. XM_006237614.2.
UniGeneiRn.11394.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2KJ7NMR-A38-74[»]
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi246637. 1 interaction.
STRINGi10116.ENSRNOP00000016614.

Proteomic databases

PaxDbiP12969.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000016614; ENSRNOP00000016614; ENSRNOG00000012417.
GeneIDi24476.
KEGGirno:24476.
UCSCiRGD:2854. rat.

Organism-specific databases

CTDi3375.
RGDi2854. Iapp.

Phylogenomic databases

eggNOGiENOG410J0NB. Eukaryota.
ENOG410Z7V7. LUCA.
GeneTreeiENSGT00510000048671.
HOGENOMiHOG000038203.
HOVERGENiHBG096065.
InParanoidiP12969.
KOiK08039.
OMAiSHQMEKR.
OrthoDBiEOG7TMZVK.
PhylomeDBiP12969.
TreeFamiTF330783.

Enzyme and pathway databases

ReactomeiR-RNO-418555. G alpha (s) signalling events.
R-RNO-419812. Calcitonin-like ligand receptors.

Miscellaneous databases

EvolutionaryTraceiP12969.
NextBioi603429.
PROiP12969.

Gene expression databases

GenevisibleiP12969. RN.

Family and domain databases

InterProiIPR021117. Calcitonin-like.
IPR021116. Calcitonin/adrenomedullin.
IPR018360. Calcitonin_CS.
IPR001693. Calcitonin_peptide-like.
IPR000443. Pro-islet_amyloid_polypep.
[Graphical view]
PANTHERiPTHR10505. PTHR10505. 1 hit.
PfamiPF00214. Calc_CGRP_IAPP. 1 hit.
[Graphical view]
PRINTSiPR00818. ISLETAMYLOID.
SMARTiSM00113. CALCITONIN. 1 hit.
[Graphical view]
PROSITEiPS00258. CALCITONIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Conservation of the sequence of islet amyloid polypeptide in five mammals is consistent with its putative role as an islet hormone."
    Nishi M., Chan S.J., Nagamatsu S., Bell G.I., Steiner D.F.
    Proc. Natl. Acad. Sci. U.S.A. 86:5738-5742(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Rat amylin: cloning and tissue-specific expression in pancreatic islets."
    Leffert J.D., Newgard C.B., Okamoto H., Milburn J.L., Luskey K.L.
    Proc. Natl. Acad. Sci. U.S.A. 86:3127-3130(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Islet amyloid polypeptide: structure and upstream sequences of the IAPP gene in rat and man."
    van Mansfeld A.D.M., Mosselman S., Hoeppener J.W.M., Zandberg J., van Teeffelen H.A.A.M., Baas P.D., Lips C.J.M., Jansz H.S.
    Biochim. Biophys. Acta 1087:235-240(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: WAP.
    Tissue: Liver.
  4. "Isolation and sequence determination of rat islet amyloid polypeptide."
    Asai J., Nakazato M., Kangawa K., Matsukura S., Matsuo H.
    Biochem. Biophys. Res. Commun. 164:400-405(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 38-74.
  5. "Regional distribution and molecular forms of rat islet amyloid polypeptide."
    Asai J., Nakazato M., Miyazato M., Kangawa K., Matsuo H., Matsukura S.
    Biochem. Biophys. Res. Commun. 169:788-795(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 38-74.
  6. "Sequence divergence in a specific region of islet amyloid polypeptide (IAPP) explains differences in islet amyloid formation between species."
    Betsholtz C., Christmansson L., Engstroem U., Rorsman F., Svensson V., Johnson K.H., Westermark P.
    FEBS Lett. 251:261-264(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 38-74.
  7. "Amyloidogenesis abolished by proline substitutions but enhanced by lipid binding."
    Jiang P., Xu W., Mu Y.
    PLoS Comput. Biol. 5:E1000357-E1000357(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: DOMAIN.
  8. "Three-dimensional structure and orientation of rat islet amyloid polypeptide protein in a membrane environment by solution NMR spectroscopy."
    Nanga R.P., Brender J.R., Xu J., Hartman K., Subramanian V., Ramamoorthy A.
    J. Am. Chem. Soc. 131:8252-8261(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 38-74, DISULFIDE BOND.

Entry informationi

Entry nameiIAPP_RAT
AccessioniPrimary (citable) accession number: P12969
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 1, 1989
Last modified: December 9, 2015
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.