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P12961

- 7B2_MOUSE

UniProt

P12961 - 7B2_MOUSE

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Protein

Neuroendocrine protein 7B2

Gene

Scg5

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Acts as a molecular chaperone for PCSK2/PC2, preventing its premature activation in the regulated secretory pathway. Binds to inactive PCSK2 in the endoplasmic reticulum and facilitates its transport from there to later compartments of the secretory pathway where it is proteolytically matured and activated. Also required for cleavage of PCSK2 but does not appear to be involved in its folding. Plays a role in regulating pituitary hormone secretion. The C-terminal peptide inhibits PCSK2 in vitro.2 Publications

GO - Molecular functioni

  1. enzyme inhibitor activity Source: UniProtKB
  2. unfolded protein binding Source: UniProtKB

GO - Biological processi

  1. intracellular protein transport Source: UniProtKB
  2. negative regulation of catalytic activity Source: GOC
  3. neuropeptide signaling pathway Source: UniProtKB-KW
  4. peptide hormone processing Source: UniProtKB
  5. regulation of hormone secretion Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Chaperone, Neuropeptide

Keywords - Biological processi

Transport

Protein family/group databases

MEROPSiI21.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Neuroendocrine protein 7B2
Alternative name(s):
Secretogranin V
Secretogranin-5
Secretory granule endocrine protein I
Cleaved into the following 2 chains:
Gene namesi
Name:Scg5
Synonyms:Sgne-1, Sgne1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 2

Organism-specific databases

MGIiMGI:98289. Scg5.

Subcellular locationi

Secreted
Note: Neuroendocrine and endocrine secretory granules.

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
  2. secretory granule Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Disruption phenotypei

Mice have no demonstrable Pcsk2/Pc2 activity, are deficient in processing islet hormones, and display hypoglycemia, hyperproinsulinemia and hypoglucagonemia, similar to Pcsk2 null mice. In contrast to Pcsk2 null mice, they develop Cushing's disease due to excessive secretion of corticotropin from the pituitary and die before 9 weeks, indicating a role for Sgne1 in control of peptide secretion from the pituitary.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi177 – 1771R → A: No effect on proteolytic processing. Abolishes proteolytic processing; when associated with G-178. 1 Publication
Mutagenesisi178 – 1781R → G: Abolishes proteolytic processing; when associated with A-177. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2626Add
BLAST
Chaini27 – 212186Neuroendocrine protein 7B2PRO_0000000044Add
BLAST
Chaini27 – 176150N-terminal peptideBy similarityPRO_0000000045Add
BLAST
Peptidei200 – 21213C-terminal peptideBy similarityPRO_0000000046Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi120 ↔ 130By similarity

Post-translational modificationi

Proteolytically cleaved in the Golgi by a furin-like convertase to generate bioactive peptides.1 Publication
Sulfated on tyrosine residues.1 Publication

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Sulfation

Proteomic databases

MaxQBiP12961.
PaxDbiP12961.
PRIDEiP12961.

PTM databases

PhosphoSiteiP12961.

Expressioni

Gene expression databases

BgeeiP12961.
CleanExiMM_SCG5.
ExpressionAtlasiP12961. baseline and differential.
GenevestigatoriP12961.

Interactioni

Subunit structurei

Interacts with PCSK2/PC2 early in the secretory pathway. Dissociation occurs at later stages (By similarity).By similarity

Protein-protein interaction databases

IntActiP12961. 1 interaction.
MINTiMINT-1508684.

Structurei

3D structure databases

ProteinModelPortaliP12961.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the 7B2 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG271457.
HOGENOMiHOG000259690.
HOVERGENiHBG000031.
InParanoidiP12961.
OMAiPAHQATN.
OrthoDBiEOG7KSX9R.
PhylomeDBiP12961.
TreeFamiTF314328.

Family and domain databases

InterProiIPR007945. Secretogranin_V.
[Graphical view]
PANTHERiPTHR12738. PTHR12738. 1 hit.
PfamiPF05281. Secretogranin_V. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P12961-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MASRLVSAML SGLLFWLMFE WNPAFAYSPR TPDRVSETDI QRLLHGVMEQ
60 70 80 90 100
LGIARPRVEY PAHQAMNLVG PQSIEGGAHE GLQHLGPFGN IPNIVAELTG
110 120 130 140 150
DNIPKDFSED QGYPDPPNPC PLGKTADDGC LENAPDTAEF SREFQLDQHL
160 170 180 190 200
FDPEHDYPGL GKWNKKLLYE KMKGGQRRKR RSVNPYLQGK RLDNVVAKKS
210
VPHFSEEEKE AE
Length:212
Mass (Da):23,866
Last modified:January 1, 1990 - v1
Checksum:i6D8CD046532F9609
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti6 – 61V → G in BAB23956. (PubMed:16141072)Curated
Sequence conflicti119 – 1191P → H in BAB30765. (PubMed:16141072)Curated
Sequence conflicti145 – 1451Q → R in BAB31669. (PubMed:16141072)Curated
Sequence conflicti153 – 1531P → T in BAC27581. (PubMed:16141072)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15830 mRNA. Translation: CAA33835.1.
AK005331 mRNA. Translation: BAB23956.1.
AK011938 mRNA. Translation: BAB27927.1.
AK017481 mRNA. Translation: BAB30765.1.
AK019337 mRNA. Translation: BAB31669.1.
AK031856 mRNA. Translation: BAC27581.1.
BC029021 mRNA. Translation: AAH29021.1.
CCDSiCCDS16561.1.
PIRiS12477.
RefSeqiNP_033188.3. NM_009162.3.
UniGeneiMm.4836.

Genome annotation databases

EnsembliENSMUST00000024005; ENSMUSP00000024005; ENSMUSG00000023236.
GeneIDi20394.
KEGGimmu:20394.
UCSCiuc008lpq.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15830 mRNA. Translation: CAA33835.1 .
AK005331 mRNA. Translation: BAB23956.1 .
AK011938 mRNA. Translation: BAB27927.1 .
AK017481 mRNA. Translation: BAB30765.1 .
AK019337 mRNA. Translation: BAB31669.1 .
AK031856 mRNA. Translation: BAC27581.1 .
BC029021 mRNA. Translation: AAH29021.1 .
CCDSi CCDS16561.1.
PIRi S12477.
RefSeqi NP_033188.3. NM_009162.3.
UniGenei Mm.4836.

3D structure databases

ProteinModelPortali P12961.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P12961. 1 interaction.
MINTi MINT-1508684.

Protein family/group databases

MEROPSi I21.001.

PTM databases

PhosphoSitei P12961.

Proteomic databases

MaxQBi P12961.
PaxDbi P12961.
PRIDEi P12961.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000024005 ; ENSMUSP00000024005 ; ENSMUSG00000023236 .
GeneIDi 20394.
KEGGi mmu:20394.
UCSCi uc008lpq.1. mouse.

Organism-specific databases

CTDi 6447.
MGIi MGI:98289. Scg5.

Phylogenomic databases

eggNOGi NOG271457.
HOGENOMi HOG000259690.
HOVERGENi HBG000031.
InParanoidi P12961.
OMAi PAHQATN.
OrthoDBi EOG7KSX9R.
PhylomeDBi P12961.
TreeFami TF314328.

Miscellaneous databases

NextBioi 298342.
PROi P12961.
SOURCEi Search...

Gene expression databases

Bgeei P12961.
CleanExi MM_SCG5.
ExpressionAtlasi P12961. baseline and differential.
Genevestigatori P12961.

Family and domain databases

InterProi IPR007945. Secretogranin_V.
[Graphical view ]
PANTHERi PTHR12738. PTHR12738. 1 hit.
Pfami PF05281. Secretogranin_V. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "cDNA sequence of neuroendocrine protein 7B2 expressed in beta cell tumors of transgenic mice."
    Mbikay M., Grant S.G.N., Sirois F., Tadros H., Skowronski J., Lazure C., Seidah N.G., Hanahan D., Chretien M.
    Int. J. Pept. Protein Res. 33:39-45(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    Strain: BALB/c.
    Tissue: Pancreas.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Cerebellum, Embryo, Hippocampus and Medulla oblongata.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Retina.
  4. "Mechanism of the facilitation of PC2 maturation by 7B2: involvement in ProPC2 transport and activation but not folding."
    Muller L., Zhu X., Lindberg I.
    J. Cell Biol. 139:625-638(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  5. "The neuroendocrine protein 7B2 is required for peptide hormone processing in vivo and provides a novel mechanism for pituitary Cushing's disease."
    Westphal C.H., Muller L., Zhou A., Zhu X., Bonner-Weir S., Schambelan M., Steiner D.F., Lindberg I., Leder P.
    Cell 96:689-700(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "The neuroendocrine polypeptide 7B2 is an endogenous inhibitor of prohormone convertase PC2."
    Martens G.J.M., Braks J.A., Eib D.W., Zhou Y., Lindberg I.
    Proc. Natl. Acad. Sci. U.S.A. 91:5784-5787(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: INHIBITION OF PCSK2 BY C-TERMINAL PEPTIDE.
  7. "The neuroendocrine precursor 7B2 is a sulfated protein proteolytically processed by a ubiquitous furin-like convertase."
    Paquet L., Bergeron F., Boudreault A., Seidah N.G., Chretien M., Mbikay M., Lazure C.
    J. Biol. Chem. 269:19279-19285(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: SULFATION, CLEAVAGE BY FURIN-LIKE CONVERTASE, MUTAGENESIS OF ARG-177 AND ARG-178.
  8. "Neuroendocrine secretory protein 7B2: structure, expression and functions."
    Mbikay M., Seidah N.G., Chretien M.
    Biochem. J. 357:329-342(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.

Entry informationi

Entry namei7B2_MOUSE
AccessioniPrimary (citable) accession number: P12961
Secondary accession number(s): Q8CCZ3
, Q9CYP0, Q9D2P6, Q9DB14
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 1, 1990
Last modified: October 29, 2014
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3