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P12961

- 7B2_MOUSE

UniProt

P12961 - 7B2_MOUSE

Protein

Neuroendocrine protein 7B2

Gene

Scg5

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 1 (01 Jan 1990)
      Previous versions | rss
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    Functioni

    Acts as a molecular chaperone for PCSK2/PC2, preventing its premature activation in the regulated secretory pathway. Binds to inactive PCSK2 in the endoplasmic reticulum and facilitates its transport from there to later compartments of the secretory pathway where it is proteolytically matured and activated. Also required for cleavage of PCSK2 but does not appear to be involved in its folding. Plays a role in regulating pituitary hormone secretion. The C-terminal peptide inhibits PCSK2 in vitro.2 Publications

    GO - Molecular functioni

    1. enzyme inhibitor activity Source: UniProtKB
    2. unfolded protein binding Source: UniProtKB

    GO - Biological processi

    1. intracellular protein transport Source: UniProtKB
    2. negative regulation of catalytic activity Source: GOC
    3. neuropeptide signaling pathway Source: UniProtKB-KW
    4. peptide hormone processing Source: UniProtKB
    5. regulation of hormone secretion Source: UniProtKB

    Keywords - Molecular functioni

    Chaperone, Neuropeptide

    Keywords - Biological processi

    Transport

    Protein family/group databases

    MEROPSiI21.001.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Neuroendocrine protein 7B2
    Alternative name(s):
    Secretogranin V
    Secretogranin-5
    Secretory granule endocrine protein I
    Cleaved into the following 2 chains:
    Gene namesi
    Name:Scg5
    Synonyms:Sgne-1, Sgne1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 2

    Organism-specific databases

    MGIiMGI:98289. Scg5.

    Subcellular locationi

    Secreted
    Note: Neuroendocrine and endocrine secretory granules.

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell
    2. secretory granule Source: UniProtKB

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Disruption phenotypei

    Mice have no demonstrable Pcsk2/Pc2 activity, are deficient in processing islet hormones, and display hypoglycemia, hyperproinsulinemia and hypoglucagonemia, similar to Pcsk2 null mice. In contrast to Pcsk2 null mice, they develop Cushing's disease due to excessive secretion of corticotropin from the pituitary and die before 9 weeks, indicating a role for Sgne1 in control of peptide secretion from the pituitary.1 Publication

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi177 – 1771R → A: No effect on proteolytic processing. Abolishes proteolytic processing; when associated with G-178. 1 Publication
    Mutagenesisi178 – 1781R → G: Abolishes proteolytic processing; when associated with A-177. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2626Add
    BLAST
    Chaini27 – 212186Neuroendocrine protein 7B2PRO_0000000044Add
    BLAST
    Chaini27 – 176150N-terminal peptideBy similarityPRO_0000000045Add
    BLAST
    Peptidei200 – 21213C-terminal peptideBy similarityPRO_0000000046Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi120 ↔ 130By similarity

    Post-translational modificationi

    Proteolytically cleaved in the Golgi by a furin-like convertase to generate bioactive peptides.1 Publication
    Sulfated on tyrosine residues.1 Publication

    Keywords - PTMi

    Cleavage on pair of basic residues, Disulfide bond, Sulfation

    Proteomic databases

    PaxDbiP12961.
    PRIDEiP12961.

    PTM databases

    PhosphoSiteiP12961.

    Expressioni

    Gene expression databases

    ArrayExpressiP12961.
    BgeeiP12961.
    CleanExiMM_SCG5.
    GenevestigatoriP12961.

    Interactioni

    Subunit structurei

    Interacts with PCSK2/PC2 early in the secretory pathway. Dissociation occurs at later stages By similarity.By similarity

    Protein-protein interaction databases

    IntActiP12961. 1 interaction.
    MINTiMINT-1508684.

    Structurei

    3D structure databases

    ProteinModelPortaliP12961.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the 7B2 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG271457.
    HOGENOMiHOG000259690.
    HOVERGENiHBG000031.
    InParanoidiP12961.
    OMAiPAHQATN.
    OrthoDBiEOG7KSX9R.
    PhylomeDBiP12961.
    TreeFamiTF314328.

    Family and domain databases

    InterProiIPR007945. Secretogranin_V.
    [Graphical view]
    PANTHERiPTHR12738. PTHR12738. 1 hit.
    PfamiPF05281. Secretogranin_V. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P12961-1 [UniParc]FASTAAdd to Basket

    « Hide

    MASRLVSAML SGLLFWLMFE WNPAFAYSPR TPDRVSETDI QRLLHGVMEQ    50
    LGIARPRVEY PAHQAMNLVG PQSIEGGAHE GLQHLGPFGN IPNIVAELTG 100
    DNIPKDFSED QGYPDPPNPC PLGKTADDGC LENAPDTAEF SREFQLDQHL 150
    FDPEHDYPGL GKWNKKLLYE KMKGGQRRKR RSVNPYLQGK RLDNVVAKKS 200
    VPHFSEEEKE AE 212
    Length:212
    Mass (Da):23,866
    Last modified:January 1, 1990 - v1
    Checksum:i6D8CD046532F9609
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti6 – 61V → G in BAB23956. (PubMed:16141072)Curated
    Sequence conflicti119 – 1191P → H in BAB30765. (PubMed:16141072)Curated
    Sequence conflicti145 – 1451Q → R in BAB31669. (PubMed:16141072)Curated
    Sequence conflicti153 – 1531P → T in BAC27581. (PubMed:16141072)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X15830 mRNA. Translation: CAA33835.1.
    AK005331 mRNA. Translation: BAB23956.1.
    AK011938 mRNA. Translation: BAB27927.1.
    AK017481 mRNA. Translation: BAB30765.1.
    AK019337 mRNA. Translation: BAB31669.1.
    AK031856 mRNA. Translation: BAC27581.1.
    BC029021 mRNA. Translation: AAH29021.1.
    CCDSiCCDS16561.1.
    PIRiS12477.
    RefSeqiNP_033188.3. NM_009162.3.
    UniGeneiMm.4836.

    Genome annotation databases

    EnsembliENSMUST00000024005; ENSMUSP00000024005; ENSMUSG00000023236.
    GeneIDi20394.
    KEGGimmu:20394.
    UCSCiuc008lpq.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X15830 mRNA. Translation: CAA33835.1 .
    AK005331 mRNA. Translation: BAB23956.1 .
    AK011938 mRNA. Translation: BAB27927.1 .
    AK017481 mRNA. Translation: BAB30765.1 .
    AK019337 mRNA. Translation: BAB31669.1 .
    AK031856 mRNA. Translation: BAC27581.1 .
    BC029021 mRNA. Translation: AAH29021.1 .
    CCDSi CCDS16561.1.
    PIRi S12477.
    RefSeqi NP_033188.3. NM_009162.3.
    UniGenei Mm.4836.

    3D structure databases

    ProteinModelPortali P12961.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P12961. 1 interaction.
    MINTi MINT-1508684.

    Protein family/group databases

    MEROPSi I21.001.

    PTM databases

    PhosphoSitei P12961.

    Proteomic databases

    PaxDbi P12961.
    PRIDEi P12961.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000024005 ; ENSMUSP00000024005 ; ENSMUSG00000023236 .
    GeneIDi 20394.
    KEGGi mmu:20394.
    UCSCi uc008lpq.1. mouse.

    Organism-specific databases

    CTDi 6447.
    MGIi MGI:98289. Scg5.

    Phylogenomic databases

    eggNOGi NOG271457.
    HOGENOMi HOG000259690.
    HOVERGENi HBG000031.
    InParanoidi P12961.
    OMAi PAHQATN.
    OrthoDBi EOG7KSX9R.
    PhylomeDBi P12961.
    TreeFami TF314328.

    Miscellaneous databases

    NextBioi 298342.
    PROi P12961.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P12961.
    Bgeei P12961.
    CleanExi MM_SCG5.
    Genevestigatori P12961.

    Family and domain databases

    InterProi IPR007945. Secretogranin_V.
    [Graphical view ]
    PANTHERi PTHR12738. PTHR12738. 1 hit.
    Pfami PF05281. Secretogranin_V. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "cDNA sequence of neuroendocrine protein 7B2 expressed in beta cell tumors of transgenic mice."
      Mbikay M., Grant S.G.N., Sirois F., Tadros H., Skowronski J., Lazure C., Seidah N.G., Hanahan D., Chretien M.
      Int. J. Pept. Protein Res. 33:39-45(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
      Strain: BALB/c.
      Tissue: Pancreas.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Cerebellum, Embryo, Hippocampus and Medulla oblongata.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Retina.
    4. "Mechanism of the facilitation of PC2 maturation by 7B2: involvement in ProPC2 transport and activation but not folding."
      Muller L., Zhu X., Lindberg I.
      J. Cell Biol. 139:625-638(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DISRUPTION PHENOTYPE.
    5. "The neuroendocrine protein 7B2 is required for peptide hormone processing in vivo and provides a novel mechanism for pituitary Cushing's disease."
      Westphal C.H., Muller L., Zhou A., Zhu X., Bonner-Weir S., Schambelan M., Steiner D.F., Lindberg I., Leder P.
      Cell 96:689-700(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. "The neuroendocrine polypeptide 7B2 is an endogenous inhibitor of prohormone convertase PC2."
      Martens G.J.M., Braks J.A., Eib D.W., Zhou Y., Lindberg I.
      Proc. Natl. Acad. Sci. U.S.A. 91:5784-5787(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: INHIBITION OF PCSK2 BY C-TERMINAL PEPTIDE.
    7. "The neuroendocrine precursor 7B2 is a sulfated protein proteolytically processed by a ubiquitous furin-like convertase."
      Paquet L., Bergeron F., Boudreault A., Seidah N.G., Chretien M., Mbikay M., Lazure C.
      J. Biol. Chem. 269:19279-19285(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: SULFATION, CLEAVAGE BY FURIN-LIKE CONVERTASE, MUTAGENESIS OF ARG-177 AND ARG-178.
    8. "Neuroendocrine secretory protein 7B2: structure, expression and functions."
      Mbikay M., Seidah N.G., Chretien M.
      Biochem. J. 357:329-342(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW.

    Entry informationi

    Entry namei7B2_MOUSE
    AccessioniPrimary (citable) accession number: P12961
    Secondary accession number(s): Q8CCZ3
    , Q9CYP0, Q9D2P6, Q9DB14
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1990
    Last sequence update: January 1, 1990
    Last modified: October 1, 2014
    This is version 116 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3