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Protein

ATP-dependent DNA helicase SRS2

Gene

SRS2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

ATP-dependent DNA helicase involved in DNA repair at least for UV-induced lesions. The polarity of the helicase activity was determined to be 3' to 5'.

Catalytic activityi

ATP + H2O = ADP + phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei314ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi38 – 43ATPPROSITE-ProRule annotation6

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ATP-dependent DNA helicase activity Source: InterPro
  • DNA binding Source: UniProtKB-KW
  • DNA helicase activity Source: SGD
  • enzyme activator activity Source: SGD

GO - Biological processi

  • DNA duplex unwinding Source: SGD
  • DNA repair Source: SGD
  • double-strand break repair via nonhomologous end joining Source: SGD
  • negative regulation of DNA recombination Source: SGD
  • negative regulation of double-strand break repair via homologous recombination Source: SGD
  • positive regulation of endodeoxyribonuclease activity Source: SGD
  • protein-DNA complex disassembly Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31547-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent DNA helicase SRS2 (EC:3.6.4.12)
Gene namesi
Name:SRS2
Synonyms:HPR5, RADH
Ordered Locus Names:YJL092W
ORF Names:J0913
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJL092W.
SGDiS000003628. SRS2.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001020711 – 1174ATP-dependent DNA helicase SRS2Add BLAST1174

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei833PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP12954.
PRIDEiP12954.

PTM databases

iPTMnetiP12954.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
POL30P1587310EBI-18110,EBI-12993
SMT3Q123064EBI-18110,EBI-17490

Protein-protein interaction databases

BioGridi33665. 499 interactors.
DIPiDIP-415N.
IntActiP12954. 6 interactors.
MINTiMINT-385280.

Structurei

Secondary structure

11174
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi1152 – 1160Combined sources9
Beta strandi1170 – 1172Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3V62X-ray2.90C/F1107-1174[»]
ProteinModelPortaliP12954.
SMRiP12954.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini14 – 316UvrD-like helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST303
Domaini317 – 654UvrD-like helicase C-terminalPROSITE-ProRule annotationAdd BLAST338

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni222 – 243Leucine-zipperAdd BLAST22

Sequence similaritiesi

Belongs to the helicase family. UvrD subfamily.Curated
Contains 1 uvrD-like helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 uvrD-like helicase C-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00530000066700.
HOGENOMiHOG000141756.
InParanoidiP12954.
KOiK03657.
OMAiYRSSQKI.
OrthoDBiEOG092C0ORX.

Family and domain databases

Gene3Di1.10.10.160. 1 hit.
3.40.50.300. 4 hits.
InterProiIPR013986. DExx_box_DNA_helicase_dom.
IPR014017. DNA_helicase_UvrD-like_C.
IPR000212. DNA_helicase_UvrD/REP.
IPR027417. P-loop_NTPase.
IPR014016. UvrD-like_ATP-bd.
[Graphical view]
PANTHERiPTHR11070. PTHR11070. 3 hits.
PfamiPF00580. UvrD-helicase. 1 hit.
PF13361. UvrD_C. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 3 hits.
PROSITEiPS51198. UVRD_HELICASE_ATP_BIND. 1 hit.
PS51217. UVRD_HELICASE_CTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P12954-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSNNDLWLH LVSQLNTQQR AAALFDYTRG LQVIAGPGTG KTKVLTSRVA
60 70 80 90 100
YLILHHHIHP RDIIVTTFTN KAANEMKERL QEMLRGAGVN ISELLIGTFH
110 120 130 140 150
SICLKILYRF GHLVDLQKDW RIIDEKEIDV ILDDMIEKVP DQIRDYASSI
160 170 180 190 200
TRKVNLCMPS KNGDEWTIHP KLIKKQISKL KSNAILPEEY ILDSNHDAAL
210 220 230 240 250
GYFYQIYQSE LSKKNTLDFD DLLMYTFRLL TRVRVLSNIK HVLVDEFQDT
260 270 280 290 300
NGIQLDLMFL FAKGNHHLSR GMTIVGDPDQ SIYAFRNALA HNFLEMGRKC
310 320 330 340 350
PIEYSTIILV ENYRSSQKIL NTSEILITQQ NKGRQNRAPL RAQFDLDFPP
360 370 380 390 400
VYMNFPAYFL EAPSLVRELL YLKALPNLFT FNDFAILVRQ RRQIKRIESA
410 420 430 440 450
LIEHRIPYKI IRGHSFWDSK ETRAMLNLLK LIFSPNDKHA ILASLLYPAR
460 470 480 490 500
GLGPATGEKI KNALDTLATD VSCFQILKDI SSKKIMLDIP TKGRSVIADF
510 520 530 540 550
ISMIENCQLL LQSTLLGGLS DLFDKLYELS GLKYEYLYKD GKKKNDQLEK
560 570 580 590 600
SEPNLLNARH KNIELLKNYF LALLSKSESS DKEKNEAIKA ATDEAEPIEN
610 620 630 640 650
KVITPKEYLR NFFNSLSLHS DAAEEEESES NKDAKIKREK NGFVTISTIH
660 670 680 690 700
GAKGLEWPVV FIPGCEEGII PCVFNDDKKD ESEEDEEEDQ ENSKKDASPK
710 720 730 740 750
KTRVLSVEDS IDEERRMFFV AQTRAKYLLY LSNTVTVEDV DRPRIASRFL
760 770 780 790 800
TTDLIKAMSD SQKLFESTNS IKKLYRILNK KPPAEDDKLF SLDQLRKDYN
810 820 830 840 850
QFIENRRERM IWQGIQMNDV YGIQLSRNKL LGSVSDFTSA ADQLRLETQN
860 870 880 890 900
SIFPQKKLIE KSRPSKINGN YAPKSRVKSP EKRYAPETTS FHSPTKKKVY
910 920 930 940 950
APQYVSTTNV PSRQEFHSST GKNIPFLRRE DRSITDISPR SSTRSLKGAS
960 970 980 990 1000
PNKTSHMSDD LMRPSPTRKD KVTRNIHFAT AGTFRIETQS NVDELHPPEY
1010 1020 1030 1040 1050
SNKSGQSLTS SEFSGFSSAC SNSDQPTNLI EDINNELDLS DEELLNDISI
1060 1070 1080 1090 1100
ERRRELLGSK KTKKIKPKTR NRKSKRGDKV KVEEVIDLKS EFEEDDSRNT
1110 1120 1130 1140 1150
TAAELLHNPD DTTVDNRPII SNAKFLADAA MKKTQKFSKK VKNEPASSQM
1160 1170
DIFSQLSRAK KKSKLNNGEI IVID
Length:1,174
Mass (Da):134,306
Last modified:December 6, 2005 - v2
Checksum:iAE8061B114C32120
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti173 – 182IKKQISKLKS → TQETDIKAKI (PubMed:2552405).Curated10
Sequence conflicti534 – 548YEYLY…KNDQL → VRILGTRMVRKKMSQF (PubMed:2552405).CuratedAdd BLAST15

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15665 Genomic DNA. Translation: CAA33706.1.
X77087 Genomic DNA. Translation: CAA54361.1.
Z49367 Genomic DNA. Translation: CAA89385.1.
BK006943 Genomic DNA. Translation: DAA08708.1.
PIRiS46586. HJBYDH.
RefSeqiNP_012443.1. NM_001181525.1.

Genome annotation databases

EnsemblFungiiYJL092W; YJL092W; YJL092W.
GeneIDi853353.
KEGGisce:YJL092W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15665 Genomic DNA. Translation: CAA33706.1.
X77087 Genomic DNA. Translation: CAA54361.1.
Z49367 Genomic DNA. Translation: CAA89385.1.
BK006943 Genomic DNA. Translation: DAA08708.1.
PIRiS46586. HJBYDH.
RefSeqiNP_012443.1. NM_001181525.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3V62X-ray2.90C/F1107-1174[»]
ProteinModelPortaliP12954.
SMRiP12954.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33665. 499 interactors.
DIPiDIP-415N.
IntActiP12954. 6 interactors.
MINTiMINT-385280.

PTM databases

iPTMnetiP12954.

Proteomic databases

MaxQBiP12954.
PRIDEiP12954.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYJL092W; YJL092W; YJL092W.
GeneIDi853353.
KEGGisce:YJL092W.

Organism-specific databases

EuPathDBiFungiDB:YJL092W.
SGDiS000003628. SRS2.

Phylogenomic databases

GeneTreeiENSGT00530000066700.
HOGENOMiHOG000141756.
InParanoidiP12954.
KOiK03657.
OMAiYRSSQKI.
OrthoDBiEOG092C0ORX.

Enzyme and pathway databases

BioCyciYEAST:G3O-31547-MONOMER.

Miscellaneous databases

PROiP12954.

Family and domain databases

Gene3Di1.10.10.160. 1 hit.
3.40.50.300. 4 hits.
InterProiIPR013986. DExx_box_DNA_helicase_dom.
IPR014017. DNA_helicase_UvrD-like_C.
IPR000212. DNA_helicase_UvrD/REP.
IPR027417. P-loop_NTPase.
IPR014016. UvrD-like_ATP-bd.
[Graphical view]
PANTHERiPTHR11070. PTHR11070. 3 hits.
PfamiPF00580. UvrD-helicase. 1 hit.
PF13361. UvrD_C. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 3 hits.
PROSITEiPS51198. UVRD_HELICASE_ATP_BIND. 1 hit.
PS51217. UVRD_HELICASE_CTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSRS2_YEAST
AccessioniPrimary (citable) accession number: P12954
Secondary accession number(s): D6VW92
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: December 6, 2005
Last modified: November 2, 2016
This is version 148 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 5190 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.