P12954 (SRS2_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ATP-dependent DNA helicase SRS2
EC=3.6.4.12

Gene names

Name:	SRS2
Synonyms:	HPR5, RADH
Ordered Locus Names:	YJL092W
ORF Names:	J0913

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	1174 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	ATP-dependent DNA helicase involved in DNA repair at least for UV-induced lesions. The polarity of the helicase activity was determined to be 3' to 5'.
Catalytic activity	ATP + H2O = ADP + phosphate.
Subcellular location	Nucleus.
Miscellaneous	Present with 5190 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Belongs to the helicase family. UvrD subfamily. Contains 1 uvrD-like helicase ATP-binding domain. Contains 1 uvrD-like helicase C-terminal domain.

Ontologies

Keywords
Biological process	DNA damage DNA repair
Cellular component	Nucleus
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Helicase Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA duplex unwinding Inferred from direct assay. Source: SGD double-strand break repair via nonhomologous end joining Inferred from direct assay. Source: SGD
Cellular component	nucleus Inferred from direct assay. Source: SGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent DNA helicase activity Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1174	1174	ATP-dependent DNA helicase SRS2		PRO_0000102071
Regions
	Domain	14 – 316	303	UvrD-like helicase ATP-binding
	Domain	222 – 243	22	Leucine-zipper
	Domain	317 – 654	338	UvrD-like helicase C-terminal
	Nucleotide binding	35 – 42	8	ATP By similarity
Amino acid modifications
	Modified residue	833	1	Phosphoserine Ref.8
	Modified residue	893	1	Phosphoserine Ref.7
	Modified residue	933	1	Phosphoserine Ref.8
Experimental info
	Sequence conflict	173 – 182	10	IKKQISKLKS → TQETDIKAKI Ref.1
	Sequence conflict	534 – 548	15	YEYLY…KNDQL → VRILGTRMVRKKMSQF Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P12954 [UniParc]. Last modified December 6, 2005. Version 2. Checksum: AE8061B114C32120	FASTA	1,174	134,306
	10 20 30 40 50 60 MSSNNDLWLH LVSQLNTQQR AAALFDYTRG LQVIAGPGTG KTKVLTSRVA YLILHHHIHP 70 80 90 100 110 120 RDIIVTTFTN KAANEMKERL QEMLRGAGVN ISELLIGTFH SICLKILYRF GHLVDLQKDW 130 140 150 160 170 180 RIIDEKEIDV ILDDMIEKVP DQIRDYASSI TRKVNLCMPS KNGDEWTIHP KLIKKQISKL 190 200 210 220 230 240 KSNAILPEEY ILDSNHDAAL GYFYQIYQSE LSKKNTLDFD DLLMYTFRLL TRVRVLSNIK 250 260 270 280 290 300 HVLVDEFQDT NGIQLDLMFL FAKGNHHLSR GMTIVGDPDQ SIYAFRNALA HNFLEMGRKC 310 320 330 340 350 360 PIEYSTIILV ENYRSSQKIL NTSEILITQQ NKGRQNRAPL RAQFDLDFPP VYMNFPAYFL 370 380 390 400 410 420 EAPSLVRELL YLKALPNLFT FNDFAILVRQ RRQIKRIESA LIEHRIPYKI IRGHSFWDSK 430 440 450 460 470 480 ETRAMLNLLK LIFSPNDKHA ILASLLYPAR GLGPATGEKI KNALDTLATD VSCFQILKDI 490 500 510 520 530 540 SSKKIMLDIP TKGRSVIADF ISMIENCQLL LQSTLLGGLS DLFDKLYELS GLKYEYLYKD 550 560 570 580 590 600 GKKKNDQLEK SEPNLLNARH KNIELLKNYF LALLSKSESS DKEKNEAIKA ATDEAEPIEN 610 620 630 640 650 660 KVITPKEYLR NFFNSLSLHS DAAEEEESES NKDAKIKREK NGFVTISTIH GAKGLEWPVV 670 680 690 700 710 720 FIPGCEEGII PCVFNDDKKD ESEEDEEEDQ ENSKKDASPK KTRVLSVEDS IDEERRMFFV 730 740 750 760 770 780 AQTRAKYLLY LSNTVTVEDV DRPRIASRFL TTDLIKAMSD SQKLFESTNS IKKLYRILNK 790 800 810 820 830 840 KPPAEDDKLF SLDQLRKDYN QFIENRRERM IWQGIQMNDV YGIQLSRNKL LGSVSDFTSA 850 860 870 880 890 900 ADQLRLETQN SIFPQKKLIE KSRPSKINGN YAPKSRVKSP EKRYAPETTS FHSPTKKKVY 910 920 930 940 950 960 APQYVSTTNV PSRQEFHSST GKNIPFLRRE DRSITDISPR SSTRSLKGAS PNKTSHMSDD 970 980 990 1000 1010 1020 LMRPSPTRKD KVTRNIHFAT AGTFRIETQS NVDELHPPEY SNKSGQSLTS SEFSGFSSAC 1030 1040 1050 1060 1070 1080 SNSDQPTNLI EDINNELDLS DEELLNDISI ERRRELLGSK KTKKIKPKTR NRKSKRGDKV 1090 1100 1110 1120 1130 1140 KVEEVIDLKS EFEEDDSRNT TAAELLHNPD DTTVDNRPII SNAKFLADAA MKKTQKFSKK 1150 1160 1170 VKNEPASSQM DIFSQLSRAK KKSKLNNGEI IVID

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"RADH, a gene of Saccharomyces cerevisiae encoding a putative DNA helicase involved in DNA repair. Characteristics of radH mutants and sequence of the gene." Aboussekhra A., Chanet R., Zgaga Z., Cassier-Chauvat C., Heude M., Fabre C.L. Nucleic Acids Res. 17:7211-7219(1989) [PubMed: 2552405] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: S288c / GRF88.
[2]	"Sequence and function analysis of a 9.46 kb fragment of Saccharomyces cerevisiae chromosome X." Miosga T., Witzel A., Zimmermann F.K. Yeast 10:965-973(1994) [PubMed: 7985424] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed: 8641269] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Purification and characterization of the SRS2 DNA helicase of the yeast Saccharomyces cerevisiae." Rong L., Klein H.L. J. Biol. Chem. 268:1252-1259(1993) [PubMed: 8419328] [Abstract] Cited for: CHARACTERIZATION.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-893, MASS SPECTROMETRY.
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-833 AND SER-933, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X15665 Genomic DNA. Translation: CAA33706.1. X77087 Genomic DNA. Translation: CAA54361.1. Z49367 Genomic DNA. Translation: CAA89385.1. BK006943 Genomic DNA. Translation: DAA08708.1.
PIR	HJBYDH. S46586.
RefSeq	NP_012443.1. NM_001181525.1.
3D structure databases
ProteinModelPortal	P12954.
SMR	P12954. Positions 9-757.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-415N.
IntAct	P12954. 8 interactions.
MINT	MINT-385280.
STRING	P12954.
Proteomic databases
PeptideAtlas	P12954.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YJL092W; YJL092W; YJL092W.
GeneID	853353.
KEGG	sce:YJL092W.
NMPDR	fig|4932.3.peg.3412.
Organism-specific databases
CYGD	YJL092w.
SGD	S000003628. HPR5.
Phylogenomic databases
eggNOG	fuNOG05288.
GeneTree	EFGT00050000006777.
HOGENOM	HBG202180.
OMA	EERRMFF.
OrthoDB	EOG470XRJ.
Gene expression databases
ArrayExpress	P12954.
Genevestigator	P12954.
GermOnline	YJL092W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR013986. DNA_helicase_DEXX-box. IPR014017. DNA_helicase_UvrD-like_C. IPR000212. DNA_helicase_UvrD/REP. IPR014016. Helicase_SF1_UvrD-rel. [Graphical view]
Gene3D	G3DSA:1.10.10.160. DNA_helicase_DEXX-box. 1 hit.
KO	K03657.
PANTHER	PTHR11070. UvrD_helicase. 1 hit.
Pfam	PF00580. UvrD-helicase. 1 hit. [Graphical view]
PROSITE	PS51198. UVRD_HELICASE_ATP_BIND. 1 hit. PS51217. UVRD_HELICASE_CTER. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	973762.

Entry information

Entry name

SRS2_YEAST

Accession

Primary (citable) accession number: P12954
Secondary accession number(s): D6VW92

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 1, 1990
Last sequence update:	December 6, 2005
Last modified:	December 14, 2011
This is version 106 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program