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Protein

N-terminal acetyltransferase A complex subunit NAT1

Gene

NAT1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Non-catalytic component of the NatA N-terminal acetyltransferase, which catalyzes acetylation of proteins beginning with Met-Ser, Met-Gly and Met-Ala. N-acetylation plays a role in normal eukaryotic translation and processing, protect against proteolytic degradation and protein turnover. NAT1 anchors ARD1 and NAT5 to the ribosome and may present the N termini of nascent polypeptides for acetylation.2 Publications

GO - Biological processi

Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:YDL040C-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
N-terminal acetyltransferase A complex subunit NAT1
Short name:
NatA complex subunit NAT1
Alternative name(s):
Amino-terminal, alpha-amino, acetyltransferase 1
Gene namesi
Name:NAT1
Synonyms:AAA1
Ordered Locus Names:YDL040C
ORF Names:D2720
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDL040C.
SGDiS000002198. NAT1.

Subcellular locationi

GO - Cellular componenti

  • NatA complex Source: SGD
  • NatB complex Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCurated
ChainiPRO_00000967392 – 854N-terminal acetyltransferase A complex subunit NAT1Add BLAST853

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineSequence analysis1
Modified residuei674PhosphoserineCombined sources1

Post-translational modificationi

The N-terminus is blocked.

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP12945.
PRIDEiP12945.

PTM databases

iPTMnetiP12945.

Interactioni

Subunit structurei

Component of the N-terminal acetyltransferase A (NatA) complex, which is composed of ARD1, NAT1 and NAT5. Can self-associate. NAT1 associates with the nascent polypeptide chain and the ribosome.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
ARD1P0734711EBI-11868,EBI-2796

Protein-protein interaction databases

BioGridi32018. 231 interactors.
DIPiDIP-6329N.
IntActiP12945. 17 interactors.
MINTiMINT-617866.

Structurei

Secondary structure

1854
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi24 – 32Combined sources9
Helixi36 – 49Combined sources14
Helixi54 – 66Combined sources13
Helixi70 – 83Combined sources14
Helixi91 – 103Combined sources13
Helixi107 – 119Combined sources13
Helixi126 – 138Combined sources13
Helixi141 – 154Combined sources14
Helixi159 – 171Combined sources13
Helixi175 – 189Combined sources15
Helixi195 – 197Combined sources3
Helixi198 – 216Combined sources19
Helixi220 – 233Combined sources14
Helixi234 – 236Combined sources3
Helixi240 – 253Combined sources14
Helixi257 – 270Combined sources14
Helixi275 – 284Combined sources10
Helixi291 – 304Combined sources14
Helixi309 – 313Combined sources5
Helixi314 – 317Combined sources4
Helixi322 – 339Combined sources18
Helixi344 – 354Combined sources11
Helixi356 – 372Combined sources17
Turni376 – 378Combined sources3
Helixi380 – 396Combined sources17
Helixi400 – 413Combined sources14
Helixi418 – 431Combined sources14
Helixi434 – 447Combined sources14
Helixi452 – 464Combined sources13
Helixi468 – 475Combined sources8
Turni478 – 480Combined sources3
Beta strandi484 – 487Combined sources4
Helixi488 – 493Combined sources6
Helixi497 – 523Combined sources27
Helixi536 – 570Combined sources35
Helixi571 – 574Combined sources4
Helixi575 – 582Combined sources8
Helixi585 – 595Combined sources11
Helixi596 – 600Combined sources5
Helixi602 – 621Combined sources20
Turni629 – 631Combined sources3
Helixi662 – 669Combined sources8
Turni674 – 676Combined sources3
Helixi683 – 686Combined sources4
Helixi691 – 698Combined sources8
Helixi700 – 706Combined sources7
Turni709 – 711Combined sources3
Helixi714 – 721Combined sources8
Turni722 – 725Combined sources4
Helixi727 – 741Combined sources15
Beta strandi743 – 745Combined sources3
Helixi746 – 758Combined sources13
Beta strandi763 – 765Combined sources3
Helixi767 – 781Combined sources15
Turni782 – 784Combined sources3
Helixi787 – 791Combined sources5
Helixi797 – 805Combined sources9
Helixi810 – 818Combined sources9
Turni819 – 822Combined sources4
Helixi827 – 837Combined sources11
Turni838 – 840Combined sources3
Helixi843 – 852Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4HNWX-ray2.80A1-854[»]
4HNYX-ray2.25A/C1-854[»]
4XNHX-ray2.10A1-854[»]
4XPDX-ray2.81A1-854[»]
4Y49X-ray3.95A/G/M1-854[»]
ProteinModelPortaliP12945.
SMRiP12945.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati20 – 53TPR 1Add BLAST34
Repeati54 – 87TPR 2Add BLAST34
Repeati91 – 124TPR 3Add BLAST34
Repeati126 – 162TPR 4Add BLAST37
Repeati241 – 274TPR 5Add BLAST34
Repeati384 – 417TPR 6Add BLAST34
Repeati452 – 485TPR 7Add BLAST34
Repeati728 – 761TPR 8Add BLAST34

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili623 – 667Sequence analysisAdd BLAST45

Sequence similaritiesi

Contains 8 TPR repeats.Curated

Keywords - Domaini

Coiled coil, Repeat, TPR repeat

Phylogenomic databases

GeneTreeiENSGT00390000007676.
HOGENOMiHOG000248749.
InParanoidiP12945.
KOiK20792.
OMAiYDSLPHR.
OrthoDBiEOG092C0JJ9.

Family and domain databases

Gene3Di1.25.40.10. 3 hits.
InterProiIPR021183. NatA_aux_su.
IPR011990. TPR-like_helical_dom.
IPR019734. TPR_repeat.
[Graphical view]
PfamiPF12569. NARP1. 2 hits.
[Graphical view]
PIRSFiPIRSF000422. N-terminal-AcTrfase-A_aux_su. 1 hit.
SMARTiSM00028. TPR. 6 hits.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 3 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P12945-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRKRSTKPK PAAKIALKKE NDQFLEALKL YEGKQYKKSL KLLDAILKKD
60 70 80 90 100
GSHVDSLALK GLDLYSVGEK DDAASYVANA IRKIEGASAS PICCHVLGIY
110 120 130 140 150
MRNTKEYKES IKWFTAALNN GSTNKQIYRD LATLQSQIGD FKNALVSRKK
160 170 180 190 200
YWEAFLGYRA NWTSLAVAQD VNGERQQAIN TLSQFEKLAE GKISDSEKYE
210 220 230 240 250
HSECLMYKND IMYKAASDNQ DKLQNVLKHL NDIEPCVFDK FGLLERKATI
260 270 280 290 300
YMKLGQLKDA SIVYRTLIKR NPDNFKYYKL LEVSLGIQGD NKLKKALYGK
310 320 330 340 350
LEQFYPRCEP PKFIPLTFLQ DKEELSKKLR EYVLPQLERG VPATFSNVKP
360 370 380 390 400
LYQRRKSKVS PLLEKIVLDY LSGLDPTQDP IPFIWTNYYL SQHFLFLKDF
410 420 430 440 450
PKAQEYIDAA LDHTPTLVEF YILKARILKH LGLMDTAAGI LEEGRQLDLQ
460 470 480 490 500
DRFINCKTVK YFLRANNIDK AVEVASLFTK NDDSVNGIKD LHLVEASWFI
510 520 530 540 550
VEQAEAYYRL YLDRKKKLDD LASLKKEVES DKSEQIANDI KENQWLVRKY
560 570 580 590 600
KGLALKRFNA IPKFYKQFED DQLDFHSYCM RKGTPRAYLE MLEWGKALYT
610 620 630 640 650
KPMYVRAMKE ASKLYFQMHD DRLKRKSDSL DENSDEIQNN GQNSSSQKKK
660 670 680 690 700
AKKEAAAMNK RKETEAKSVA AYPSDQDNDV FGEKLIETST PMEDFATEFY
710 720 730 740 750
NNYSMQVRED ERDYILDFEF NYRIGKLALC FASLNKFAKR FGTTSGLFGS
760 770 780 790 800
MAIVLLHATR NDTPFDPILK KVVTKSLEKE YSENFPLNEI SNNSFDWLNF
810 820 830 840 850
YQEKFGKNDI NGLLFLYRYR DDVPIGSSNL KEMIISSLSP LEPHSQNEIL

QYYL
Length:854
Mass (Da):98,905
Last modified:January 23, 2007 - v2
Checksum:iDCFB870D049F91E6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M23166 mRNA. Translation: AAA88728.1.
X15135 Genomic DNA. Translation: CAA33233.1.
Z71781 Genomic DNA. Translation: CAA96449.1.
Z74088 Genomic DNA. Translation: CAA98599.1.
BK006938 Genomic DNA. Translation: DAA11815.1.
PIRiS05783. XYBYT1.
RefSeqiNP_010244.1. NM_001180099.1.

Genome annotation databases

EnsemblFungiiYDL040C; YDL040C; YDL040C.
GeneIDi851521.
KEGGisce:YDL040C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M23166 mRNA. Translation: AAA88728.1.
X15135 Genomic DNA. Translation: CAA33233.1.
Z71781 Genomic DNA. Translation: CAA96449.1.
Z74088 Genomic DNA. Translation: CAA98599.1.
BK006938 Genomic DNA. Translation: DAA11815.1.
PIRiS05783. XYBYT1.
RefSeqiNP_010244.1. NM_001180099.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4HNWX-ray2.80A1-854[»]
4HNYX-ray2.25A/C1-854[»]
4XNHX-ray2.10A1-854[»]
4XPDX-ray2.81A1-854[»]
4Y49X-ray3.95A/G/M1-854[»]
ProteinModelPortaliP12945.
SMRiP12945.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32018. 231 interactors.
DIPiDIP-6329N.
IntActiP12945. 17 interactors.
MINTiMINT-617866.

PTM databases

iPTMnetiP12945.

Proteomic databases

MaxQBiP12945.
PRIDEiP12945.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDL040C; YDL040C; YDL040C.
GeneIDi851521.
KEGGisce:YDL040C.

Organism-specific databases

EuPathDBiFungiDB:YDL040C.
SGDiS000002198. NAT1.

Phylogenomic databases

GeneTreeiENSGT00390000007676.
HOGENOMiHOG000248749.
InParanoidiP12945.
KOiK20792.
OMAiYDSLPHR.
OrthoDBiEOG092C0JJ9.

Enzyme and pathway databases

BioCyciYEAST:YDL040C-MONOMER.

Miscellaneous databases

PROiP12945.

Family and domain databases

Gene3Di1.25.40.10. 3 hits.
InterProiIPR021183. NatA_aux_su.
IPR011990. TPR-like_helical_dom.
IPR019734. TPR_repeat.
[Graphical view]
PfamiPF12569. NARP1. 2 hits.
[Graphical view]
PIRSFiPIRSF000422. N-terminal-AcTrfase-A_aux_su. 1 hit.
SMARTiSM00028. TPR. 6 hits.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 3 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiNAT1_YEAST
AccessioniPrimary (citable) accession number: P12945
Secondary accession number(s): D6VRV5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1990
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 147 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 7600 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.