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Protein

Bowman-Birk type trypsin inhibitor

Gene

BBBI

Organism
Hordeum vulgare (Barley)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

This inhibitor interacts with two molecules of trypsin.

Cofactori

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei17 – 182Reactive bond
Sitei75 – 762Reactive bond

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Serine protease inhibitor

Keywords - Ligandi

Calcium

Protein family/group databases

MEROPSiI12.009.

Names & Taxonomyi

Protein namesi
Recommended name:
Bowman-Birk type trypsin inhibitor
Gene namesi
Name:BBBI
OrganismiHordeum vulgare (Barley)
Taxonomic identifieri4513 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladePooideaeTriticodaeTriticeaeHordeinaeHordeum

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 124124Bowman-Birk type trypsin inhibitorPRO_0000105857Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi9 ↔ 622 Publications
Disulfide bondi10 ↔ 252 Publications
Disulfide bondi15 ↔ 232 Publications
Disulfide bondi32 ↔ 392 Publications
Disulfide bondi36 ↔ 502 Publications
Disulfide bondi67 ↔ 1212 Publications
Disulfide bondi68 ↔ 832 Publications
Disulfide bondi73 ↔ 812 Publications
Disulfide bondi90 ↔ 972 Publications
Disulfide bondi94 ↔ 1092 Publications

Keywords - PTMi

Disulfide bond

Structurei

Secondary structure

1
124
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi11 – 155Combined sources
Beta strandi17 – 204Combined sources
Beta strandi22 – 254Combined sources
Beta strandi39 – 413Combined sources
Beta strandi44 – 507Combined sources
Beta strandi53 – 564Combined sources
Beta strandi62 – 643Combined sources
Beta strandi69 – 735Combined sources
Beta strandi75 – 784Combined sources
Beta strandi80 – 834Combined sources
Beta strandi87 – 893Combined sources
Beta strandi97 – 993Combined sources
Beta strandi101 – 1033Combined sources
Beta strandi107 – 1093Combined sources
Beta strandi113 – 1164Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1C2AX-ray1.90A4-122[»]
1TX6X-ray2.20I/J1-124[»]
2FJ8X-ray1.19A1-124[»]
ProteinModelPortaliP12940.
SMRiP12940. Positions 4-122.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP12940.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati1 – 6262Add
BLAST
Repeati63 – 12462Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni70 – 8011Calcium bindingAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410JW1A. Eukaryota.
ENOG41105CZ. LUCA.

Family and domain databases

Gene3Di2.10.69.10. 2 hits.
InterProiIPR000877. Prot_inh_BBI.
[Graphical view]
PfamiPF00228. Bowman-Birk_leg. 3 hits.
[Graphical view]
SMARTiSM00269. BowB. 2 hits.
[Graphical view]
SUPFAMiSSF57247. SSF57247. 2 hits.
PROSITEiPS00281. BOWMAN_BIRK. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P12940-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
AGKKRPWKCC DEAVCTRSIP PICTCMDEVF ECPKTCKSCG PMGDPSRRIC
60 70 80 90 100
QDQYVGDPGP ICRPWECCDK AICTRSNPPT CRCVDEVKKC APTCKTCLPS
110 120
RSRPSRRVCI DSYFGPVPPR CTPR
Length:124
Mass (Da):13,823
Last modified:October 1, 1989 - v1
Checksum:i04CCA1CC80D2FEAD
GO

Sequence databases

PIRiJT0235. TIBHB.

Cross-referencesi

Sequence databases

PIRiJT0235. TIBHB.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1C2AX-ray1.90A4-122[»]
1TX6X-ray2.20I/J1-124[»]
2FJ8X-ray1.19A1-124[»]
ProteinModelPortaliP12940.
SMRiP12940. Positions 4-122.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiI12.009.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG410JW1A. Eukaryota.
ENOG41105CZ. LUCA.

Miscellaneous databases

EvolutionaryTraceiP12940.

Family and domain databases

Gene3Di2.10.69.10. 2 hits.
InterProiIPR000877. Prot_inh_BBI.
[Graphical view]
PfamiPF00228. Bowman-Birk_leg. 3 hits.
[Graphical view]
SMARTiSM00269. BowB. 2 hits.
[Graphical view]
SUPFAMiSSF57247. SSF57247. 2 hits.
PROSITEiPS00281. BOWMAN_BIRK. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The amino acid sequence of barley rootlet trypsin inhibitor."
    Nagasue A., Fukamachi H., Ikenaga H., Funatsu G.
    Agric. Biol. Chem. 52:1505-1514(1988)
    Cited for: PROTEIN SEQUENCE.
    Strain: cv. Distichum.
  2. "Crystal structure of a 16 kDa double-headed Bowman-Birk trypsin inhibitor from barley seeds at 1.9 A resolution."
    Song H.K., Kim Y.S., Yang J.K., Moon J., Lee J.Y., Suh S.W.
    J. Mol. Biol. 293:1133-1144(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS) OF 4-122, DISULFIDE BONDS.
  3. "Crystal structure of the Bowman-Birk inhibitor from barley seeds in ternary complex with porcine trypsin."
    Park E.Y., Kim J.-A., Kim H.-W., Kim Y.S., Song H.K.
    J. Mol. Biol. 343:173-186(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.20 ANGSTROMS), DISULFIDE BONDS.

Entry informationi

Entry nameiIBB_HORVU
AccessioniPrimary (citable) accession number: P12940
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 1, 1989
Last modified: February 17, 2016
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.