P12939 (CP2DA_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytochrome P450 2D10
EC=1.14.14.1

Alternative name(s):
CYPIID10
Cytochrome P450-CMF1B
Cytochrome P450-DB5
Debrisoquine 4-hydroxylase

Gene names

Name:	Cyp2d10
Synonyms:	Cyp2d-10, Cyp2d5

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	504 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
Catalytic activity	RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.
Cofactor	Heme group By similarity.
Subcellular location	Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
Induction	P450 can be induced to high levels in liver and other tissues by various foreign compounds, including drugs, pesticides, and carcinogens.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	aromatase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro monooxygenase activity Traceable author statement Ref.3. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 504	504	Cytochrome P450 2D10		PRO_0000051736
Sites
	Metal binding	446	1	Iron (heme axial ligand)

Sequences

	Sequence		Length	Mass (Da)
	P12939 [UniParc]. Last modified October 1, 1989. Version 1. Checksum: F308EE06F2605DFE	FASTA	504	57,076
	10 20 30 40 50 60 MELLNGTGLW PMAIFTVIFI LLVDLMHRHQ RWTSRYPPGP VPWPVLGNLL QVDPSNMPYS 70 80 90 100 110 120 MYKLQHRYGD VFSLQMGWKP MVIVNRLKAV QEVLVTHGED TADRPPVPIF KCLGVKPRSQ 130 140 150 160 170 180 GVVFASYGPE WREQRRFSVS TLRTFGMGKK SLEEWVTKEA GHLCDAFTAQ NGRSINPKAM 190 200 210 220 230 240 LNKALCNVIA SLIFARRFEY EDPYLIRMLT LVEESLIEVS GFIPEVLNTF PALLRIPGLA 250 260 270 280 290 300 DKVFQGQKTF MAFLDNLLAE NRTTWDPAQP PRNLTDAFLA EVEKAKGNPE SSFNDENLRM 310 320 330 340 350 360 VVVDLFTAGM VTTATTLTWA LLLMILYPDV QRRVQQEIDE VIGQVRCPEM TDQAHMPYTN 370 380 390 400 410 420 AVIHEVQRFG DIAPLNLPRI TSCDIEVQDF VIPKGTTLII NLSSVLKDET VWEKPLRFHP 430 440 450 460 470 480 EHFLDAQGNF VKHEAFMPFS AGRRACLGEP LARMELFLFF TCLLQHFSFS VPAGQPRPST 490 500 LGNFAISVAP LPYQLCAAVR EQGH

References

[1]	"The CYP2D gene subfamily: analysis of the molecular basis of the debrisoquine 4-hydroxylase deficiency in DA rats." Matsunaga E., Zanger U.M., Hardwick J.P., Gelboin H.V., Meyer U.A., Gonzalez F.J. Biochemistry 28:7349-7355(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Cytochrome P450CMF cDNA: nucleotide sequence of P450CMF1b." Ishida N., Inuzuka C., Tawaragi Y., Sugita O., Nakazato H., Noguchi T., Sassa S., Kappas A. Nucleic Acids Res. 17:6407-6407(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"The rat P450 IID subfamily: complete sequences of four closely linked genes and evidence that gene conversions maintained sequence homogeneity at the heme-binding region of the cytochrome P450 active site." Matsunaga E., Umeno M., Gonzalez F.J. J. Mol. Evol. 30:155-169(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Sprague-Dawley. Tissue: Liver.
[4]	"Four species of cDNAs for cytochrome P450 isozymes immunorelated to P450C-M/F encode for members of P450IID subfamily, increasing the number of members within the subfamily." Ishida N., Tawaragi Y., Inuzuka C., Sugita O., Kubota I., Nakazato H., Noguchi T., Sassa S. Biochem. Biophys. Res. Commun. 156:681-688(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 18-504.

Cross-references

Sequence databases
EMBL GenBank DDBJ	J02869 mRNA. Translation: AAA41003.1. M25143 mRNA. Translation: AAA41034.1. X52030 Genomic DNA. Translation: CAA36272.1. M22329 mRNA. Translation: AAA41045.1.
IPI	IPI00202584.
PIR	O4RTD5. S09611.
RefSeq	NP_775426.1. NM_173304.2.
UniGene	Rn.10842.
3D structure databases
ProteinModelPortal	P12939.
SMR	P12939. Positions 37-500.
ModBase	Search...
Protein-protein interaction databases
IntAct	P12939. 1 interaction.
STRING	10116.ENSRNOP00000040266.
PTM databases
PhosphoSite	P12939.
Proteomic databases
PaxDb	P12939.
PRIDE	P12939.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	286963.
KEGG	rno:286963.
UCSC	RGD:628630. rat.
Organism-specific databases
CTD	286963.
RGD	628630. Cyp2d5.
Phylogenomic databases
eggNOG	COG2124.
HOGENOM	HOG000036991.
HOVERGEN	HBG015789.
InParanoid	P12939.
KO	K07414.
OrthoDB	EOG40VVPH.
Gene expression databases
Genevestigator	P12939.
GermOnline	ENSRNOG00000029128. Rattus norvegicus.
Family and domain databases
Gene3D	1.10.630.10. 1 hit.
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. IPR008069. Cyt_P450_E_grp-I_CYP2D-like. [Graphical view]
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR01686. EP450ICYP2D. PR00385. P450.
SUPFAM	SSF48264. Cytochrome_P450. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	625171.

Entry information

Entry name

CP2DA_RAT

Accession

Primary (citable) accession number: P12939

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	October 1, 1989
Last modified:	April 3, 2013
This is version 108 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program