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P12939 (CP2DA_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 115. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cytochrome P450 2D10

EC=1.14.14.1
Alternative name(s):
CYPIID10
Cytochrome P450-CMF1B
Cytochrome P450-DB5
Debrisoquine 4-hydroxylase
Gene names
Name:Cyp2d10
Synonyms:Cyp2d-10, Cyp2d5
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length504 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.

Catalytic activity

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactor

Heme group By similarity.

Subcellular location

Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.

Induction

P450 can be induced to high levels in liver and other tissues by various foreign compounds, including drugs, pesticides, and carcinogens.

Sequence similarities

Belongs to the cytochrome P450 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 504504Cytochrome P450 2D10
PRO_0000051736

Sites

Metal binding4461Iron (heme axial ligand)

Sequences

Sequence LengthMass (Da)Tools
P12939 [UniParc].

Last modified October 1, 1989. Version 1.
Checksum: F308EE06F2605DFE

FASTA50457,076
        10         20         30         40         50         60 
MELLNGTGLW PMAIFTVIFI LLVDLMHRHQ RWTSRYPPGP VPWPVLGNLL QVDPSNMPYS 

        70         80         90        100        110        120 
MYKLQHRYGD VFSLQMGWKP MVIVNRLKAV QEVLVTHGED TADRPPVPIF KCLGVKPRSQ 

       130        140        150        160        170        180 
GVVFASYGPE WREQRRFSVS TLRTFGMGKK SLEEWVTKEA GHLCDAFTAQ NGRSINPKAM 

       190        200        210        220        230        240 
LNKALCNVIA SLIFARRFEY EDPYLIRMLT LVEESLIEVS GFIPEVLNTF PALLRIPGLA 

       250        260        270        280        290        300 
DKVFQGQKTF MAFLDNLLAE NRTTWDPAQP PRNLTDAFLA EVEKAKGNPE SSFNDENLRM 

       310        320        330        340        350        360 
VVVDLFTAGM VTTATTLTWA LLLMILYPDV QRRVQQEIDE VIGQVRCPEM TDQAHMPYTN 

       370        380        390        400        410        420 
AVIHEVQRFG DIAPLNLPRI TSCDIEVQDF VIPKGTTLII NLSSVLKDET VWEKPLRFHP 

       430        440        450        460        470        480 
EHFLDAQGNF VKHEAFMPFS AGRRACLGEP LARMELFLFF TCLLQHFSFS VPAGQPRPST 

       490        500 
LGNFAISVAP LPYQLCAAVR EQGH 

« Hide

References

[1]"The CYP2D gene subfamily: analysis of the molecular basis of the debrisoquine 4-hydroxylase deficiency in DA rats."
Matsunaga E., Zanger U.M., Hardwick J.P., Gelboin H.V., Meyer U.A., Gonzalez F.J.
Biochemistry 28:7349-7355(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Cytochrome P450CMF cDNA: nucleotide sequence of P450CMF1b."
Ishida N., Inuzuka C., Tawaragi Y., Sugita O., Nakazato H., Noguchi T., Sassa S., Kappas A.
Nucleic Acids Res. 17:6407-6407(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"The rat P450 IID subfamily: complete sequences of four closely linked genes and evidence that gene conversions maintained sequence homogeneity at the heme-binding region of the cytochrome P450 active site."
Matsunaga E., Umeno M., Gonzalez F.J.
J. Mol. Evol. 30:155-169(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Sprague-Dawley.
Tissue: Liver.
[4]"Four species of cDNAs for cytochrome P450 isozymes immunorelated to P450C-M/F encode for members of P450IID subfamily, increasing the number of members within the subfamily."
Ishida N., Tawaragi Y., Inuzuka C., Sugita O., Kubota I., Nakazato H., Noguchi T., Sassa S.
Biochem. Biophys. Res. Commun. 156:681-688(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 18-504.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
J02869 mRNA. Translation: AAA41003.1.
M25143 mRNA. Translation: AAA41034.1.
X52030 Genomic DNA. Translation: CAA36272.1.
M22329 mRNA. Translation: AAA41045.1.
PIRO4RTD5. S09611.
RefSeqNP_775426.1. NM_173304.2.
UniGeneRn.10842.

3D structure databases

ProteinModelPortalP12939.
SMRP12939. Positions 37-500.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP12939. 1 interaction.
MINTMINT-4564031.
STRING10116.ENSRNOP00000040266.

PTM databases

PhosphoSiteP12939.

Proteomic databases

PaxDbP12939.
PRIDEP12939.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID286963.
KEGGrno:286963.
UCSCRGD:628630. rat.

Organism-specific databases

CTD286963.
RGD628630. Cyp2d5.

Phylogenomic databases

eggNOGCOG2124.
HOGENOMHOG000036991.
HOVERGENHBG015789.
InParanoidP12939.
KOK07414.
PhylomeDBP12939.

Gene expression databases

GenevestigatorP12939.

Family and domain databases

Gene3D1.10.630.10. 1 hit.
InterProIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008069. Cyt_P450_E_grp-I_CYP2D-like.
[Graphical view]
PfamPF00067. p450. 1 hit.
[Graphical view]
PRINTSPR00463. EP450I.
PR01686. EP450ICYP2D.
PR00385. P450.
SUPFAMSSF48264. SSF48264. 1 hit.
PROSITEPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio625171.

Entry information

Entry nameCP2DA_RAT
AccessionPrimary (citable) accession number: P12939
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 1, 1989
Last modified: April 16, 2014
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families