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P12938

- CP2D3_RAT

UniProt

P12938 - CP2D3_RAT

Protein

Cytochrome P450 2D3

Gene

Cyp2d3

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 119 (01 Oct 2014)
      Sequence version 2 (15 Dec 1998)
      Previous versions | rss
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    Functioni

    Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.

    Catalytic activityi

    RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

    Cofactori

    Heme group.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi446 – 4461Iron (heme axial ligand)

    GO - Molecular functioni

    1. aromatase activity Source: UniProtKB-EC
    2. heme binding Source: InterPro
    3. iron ion binding Source: InterPro
    4. monooxygenase activity Source: RGD

    GO - Biological processi

    1. liver development Source: RGD

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase

    Keywords - Ligandi

    Heme, Iron, Metal-binding

    Enzyme and pathway databases

    SABIO-RKP12938.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytochrome P450 2D3 (EC:1.14.14.1)
    Alternative name(s):
    CYPIID3
    Cytochrome P450-DB3
    Debrisoquine 4-hydroxylase
    Gene namesi
    Name:Cyp2d3
    Synonyms:Cyp2d-3
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi2472. Cyp2d3.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Microsome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 500500Cytochrome P450 2D3PRO_0000051729Add
    BLAST

    Proteomic databases

    PaxDbiP12938.
    PRIDEiP12938.

    PTM databases

    PhosphoSiteiP12938.

    Expressioni

    Inductioni

    P450 can be induced to high levels in liver and other tissues by various foreign compounds, including drugs, pesticides, and carcinogens.

    Gene expression databases

    GenevestigatoriP12938.

    Interactioni

    Protein-protein interaction databases

    MINTiMINT-4577818.
    STRINGi10116.ENSRNOP00000048711.

    Structurei

    3D structure databases

    ProteinModelPortaliP12938.
    SMRiP12938. Positions 37-500.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytochrome P450 family.Curated

    Phylogenomic databases

    eggNOGiCOG2124.
    HOGENOMiHOG000036991.
    HOVERGENiHBG015789.
    InParanoidiP12938.
    KOiK07414.
    PhylomeDBiP12938.

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiIPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    IPR008069. Cyt_P450_E_grp-I_CYP2D-like.
    [Graphical view]
    PfamiPF00067. p450. 1 hit.
    [Graphical view]
    PRINTSiPR00463. EP450I.
    PR01686. EP450ICYP2D.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.
    PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P12938-1 [UniParc]FASTAAdd to Basket

    « Hide

    MELLAGTGLW PMAIFTVIFI LLVDLMHRRQ RWTSRYPPGP VPWPVLGNLL    50
    QVDLCNMPYS MYKLQNRYGD VFSLQMGWKP VVVINGLKAV QELLVTCGED 100
    TADRPEMPIF QHIGYGHKAK GVVLAPYGPE WREQRRFSVS TLRNFGVGKK 150
    SLEQWVTDEA SHLCDALTAE AGRPLDPYTL LNKAVCNVIA SLIYARRFDY 200
    GDPDFIKVLK ILKESMGEQT GLFPEVLNMF PVLLRIPGLA DKVFPGQKTF 250
    LTMVDNLVTE HKKTWDPDQP PRDLTDAFLA EIEKAKGNPE SSFNDANLRL 300
    VVNDLFGAGM VTTSITLTWA LLLMILHPDV QCRVQQEIDE VIGQVRHPEM 350
    ADQAHMPFTN AVIHEVQRFA DIVPMNLPHK TSRDIEVQGF LIPKGTTLIP 400
    NLSSVLKDET VWEKPLRFHP EHFLDAQGNF VKHEAFMPFS AGRRACLGEP 450
    LARMELFLFF TCLLQRFSFS VPTGQPRPSD YGVFAFLLSP SPYQLCAFKR 500
    Length:500
    Mass (Da):56,642
    Last modified:December 15, 1998 - v2
    Checksum:iC54727C2C00F73F6
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti125 – 1262AP → CT in AAA41002. (PubMed:2819073)Curated
    Sequence conflicti125 – 1262AP → CT in CAA36270. (PubMed:2107330)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J02868 mRNA. Translation: AAA41002.1.
    X52028 Genomic DNA. Translation: CAA36270.1.
    AB008424 mRNA. Translation: BAA23124.1.
    BC089769 mRNA. Translation: AAH89769.1.
    PIRiS16872.
    RefSeqiNP_775116.1. NM_173093.1.
    UniGeneiRn.32106.

    Genome annotation databases

    GeneIDi24303.
    KEGGirno:24303.
    UCSCiRGD:2472. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J02868 mRNA. Translation: AAA41002.1 .
    X52028 Genomic DNA. Translation: CAA36270.1 .
    AB008424 mRNA. Translation: BAA23124.1 .
    BC089769 mRNA. Translation: AAH89769.1 .
    PIRi S16872.
    RefSeqi NP_775116.1. NM_173093.1.
    UniGenei Rn.32106.

    3D structure databases

    ProteinModelPortali P12938.
    SMRi P12938. Positions 37-500.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-4577818.
    STRINGi 10116.ENSRNOP00000048711.

    Chemistry

    BindingDBi P12938.
    ChEMBLi CHEMBL3057.

    PTM databases

    PhosphoSitei P12938.

    Proteomic databases

    PaxDbi P12938.
    PRIDEi P12938.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 24303.
    KEGGi rno:24303.
    UCSCi RGD:2472. rat.

    Organism-specific databases

    CTDi 24303.
    RGDi 2472. Cyp2d3.

    Phylogenomic databases

    eggNOGi COG2124.
    HOGENOMi HOG000036991.
    HOVERGENi HBG015789.
    InParanoidi P12938.
    KOi K07414.
    PhylomeDBi P12938.

    Enzyme and pathway databases

    SABIO-RK P12938.

    Miscellaneous databases

    NextBioi 602923.

    Gene expression databases

    Genevestigatori P12938.

    Family and domain databases

    Gene3Di 1.10.630.10. 1 hit.
    InterProi IPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    IPR008069. Cyt_P450_E_grp-I_CYP2D-like.
    [Graphical view ]
    Pfami PF00067. p450. 1 hit.
    [Graphical view ]
    PRINTSi PR00463. EP450I.
    PR01686. EP450ICYP2D.
    PR00385. P450.
    SUPFAMi SSF48264. SSF48264. 1 hit.
    PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The CYP2D gene subfamily: analysis of the molecular basis of the debrisoquine 4-hydroxylase deficiency in DA rats."
      Matsunaga E., Zanger U.M., Hardwick J.P., Gelboin H.V., Meyer U.A., Gonzalez F.J.
      Biochemistry 28:7349-7355(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The rat P450 IID subfamily: complete sequences of four closely linked genes and evidence that gene conversions maintained sequence homogeneity at the heme-binding region of the cytochrome P450 active site."
      Matsunaga E., Umeno M., Gonzalez F.J.
      J. Mol. Evol. 30:155-169(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: Sprague-Dawley.
      Tissue: Liver.
    3. "Expression of four rat CYP2D isoforms in Saccharomyces cerevisiae and their catalytic specificity."
      Wan J., Imaoka S., Chow T., Hiroi T., Yabusaki Y., Funae Y.
      Arch. Biochem. Biophys. 348:383-390(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: Sprague-Dawley.
      Tissue: Liver.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Liver.

    Entry informationi

    Entry nameiCP2D3_RAT
    AccessioniPrimary (citable) accession number: P12938
    Secondary accession number(s): O35106, Q5FVU3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1989
    Last sequence update: December 15, 1998
    Last modified: October 1, 2014
    This is version 119 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3