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P12938

- CP2D3_RAT

UniProt

P12938 - CP2D3_RAT

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Protein

Cytochrome P450 2D3

Gene

Cyp2d3

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.

Catalytic activityi

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactori

Heme group.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi446 – 4461Iron (heme axial ligand)

GO - Molecular functioni

  1. aromatase activity Source: UniProtKB-EC
  2. heme binding Source: InterPro
  3. iron ion binding Source: InterPro
  4. monooxygenase activity Source: RGD

GO - Biological processi

  1. liver development Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

SABIO-RKP12938.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 2D3 (EC:1.14.14.1)
Alternative name(s):
CYPIID3
Cytochrome P450-DB3
Debrisoquine 4-hydroxylase
Gene namesi
Name:Cyp2d3
Synonyms:Cyp2d-3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi2472. Cyp2d3.

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB-KW
  2. membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 500500Cytochrome P450 2D3PRO_0000051729Add
BLAST

Proteomic databases

PaxDbiP12938.
PRIDEiP12938.

PTM databases

PhosphoSiteiP12938.

Expressioni

Inductioni

P450 can be induced to high levels in liver and other tissues by various foreign compounds, including drugs, pesticides, and carcinogens.

Gene expression databases

ExpressionAtlasiP12938. baseline and differential.
GenevestigatoriP12938.

Interactioni

Protein-protein interaction databases

MINTiMINT-4577818.
STRINGi10116.ENSRNOP00000048711.

Structurei

3D structure databases

ProteinModelPortaliP12938.
SMRiP12938. Positions 37-500.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

eggNOGiCOG2124.
HOGENOMiHOG000036991.
HOVERGENiHBG015789.
InParanoidiP12938.
KOiK07414.
PhylomeDBiP12938.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008069. Cyt_P450_E_grp-I_CYP2D-like.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR01686. EP450ICYP2D.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P12938-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MELLAGTGLW PMAIFTVIFI LLVDLMHRRQ RWTSRYPPGP VPWPVLGNLL
60 70 80 90 100
QVDLCNMPYS MYKLQNRYGD VFSLQMGWKP VVVINGLKAV QELLVTCGED
110 120 130 140 150
TADRPEMPIF QHIGYGHKAK GVVLAPYGPE WREQRRFSVS TLRNFGVGKK
160 170 180 190 200
SLEQWVTDEA SHLCDALTAE AGRPLDPYTL LNKAVCNVIA SLIYARRFDY
210 220 230 240 250
GDPDFIKVLK ILKESMGEQT GLFPEVLNMF PVLLRIPGLA DKVFPGQKTF
260 270 280 290 300
LTMVDNLVTE HKKTWDPDQP PRDLTDAFLA EIEKAKGNPE SSFNDANLRL
310 320 330 340 350
VVNDLFGAGM VTTSITLTWA LLLMILHPDV QCRVQQEIDE VIGQVRHPEM
360 370 380 390 400
ADQAHMPFTN AVIHEVQRFA DIVPMNLPHK TSRDIEVQGF LIPKGTTLIP
410 420 430 440 450
NLSSVLKDET VWEKPLRFHP EHFLDAQGNF VKHEAFMPFS AGRRACLGEP
460 470 480 490 500
LARMELFLFF TCLLQRFSFS VPTGQPRPSD YGVFAFLLSP SPYQLCAFKR
Length:500
Mass (Da):56,642
Last modified:December 15, 1998 - v2
Checksum:iC54727C2C00F73F6
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti125 – 1262AP → CT in AAA41002. (PubMed:2819073)Curated
Sequence conflicti125 – 1262AP → CT in CAA36270. (PubMed:2107330)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J02868 mRNA. Translation: AAA41002.1.
X52028 Genomic DNA. Translation: CAA36270.1.
AB008424 mRNA. Translation: BAA23124.1.
BC089769 mRNA. Translation: AAH89769.1.
PIRiS16872.
RefSeqiNP_775116.1. NM_173093.1.
UniGeneiRn.32106.

Genome annotation databases

GeneIDi24303.
KEGGirno:24303.
UCSCiRGD:2472. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J02868 mRNA. Translation: AAA41002.1 .
X52028 Genomic DNA. Translation: CAA36270.1 .
AB008424 mRNA. Translation: BAA23124.1 .
BC089769 mRNA. Translation: AAH89769.1 .
PIRi S16872.
RefSeqi NP_775116.1. NM_173093.1.
UniGenei Rn.32106.

3D structure databases

ProteinModelPortali P12938.
SMRi P12938. Positions 37-500.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

MINTi MINT-4577818.
STRINGi 10116.ENSRNOP00000048711.

Chemistry

BindingDBi P12938.
ChEMBLi CHEMBL3057.

PTM databases

PhosphoSitei P12938.

Proteomic databases

PaxDbi P12938.
PRIDEi P12938.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 24303.
KEGGi rno:24303.
UCSCi RGD:2472. rat.

Organism-specific databases

CTDi 24303.
RGDi 2472. Cyp2d3.

Phylogenomic databases

eggNOGi COG2124.
HOGENOMi HOG000036991.
HOVERGENi HBG015789.
InParanoidi P12938.
KOi K07414.
PhylomeDBi P12938.

Enzyme and pathway databases

SABIO-RK P12938.

Miscellaneous databases

NextBioi 602923.

Gene expression databases

ExpressionAtlasi P12938. baseline and differential.
Genevestigatori P12938.

Family and domain databases

Gene3Di 1.10.630.10. 1 hit.
InterProi IPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008069. Cyt_P450_E_grp-I_CYP2D-like.
[Graphical view ]
Pfami PF00067. p450. 1 hit.
[Graphical view ]
PRINTSi PR00463. EP450I.
PR01686. EP450ICYP2D.
PR00385. P450.
SUPFAMi SSF48264. SSF48264. 1 hit.
PROSITEi PS00086. CYTOCHROME_P450. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The CYP2D gene subfamily: analysis of the molecular basis of the debrisoquine 4-hydroxylase deficiency in DA rats."
    Matsunaga E., Zanger U.M., Hardwick J.P., Gelboin H.V., Meyer U.A., Gonzalez F.J.
    Biochemistry 28:7349-7355(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The rat P450 IID subfamily: complete sequences of four closely linked genes and evidence that gene conversions maintained sequence homogeneity at the heme-binding region of the cytochrome P450 active site."
    Matsunaga E., Umeno M., Gonzalez F.J.
    J. Mol. Evol. 30:155-169(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Sprague-Dawley.
    Tissue: Liver.
  3. "Expression of four rat CYP2D isoforms in Saccharomyces cerevisiae and their catalytic specificity."
    Wan J., Imaoka S., Chow T., Hiroi T., Yabusaki Y., Funae Y.
    Arch. Biochem. Biophys. 348:383-390(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Sprague-Dawley.
    Tissue: Liver.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Liver.

Entry informationi

Entry nameiCP2D3_RAT
AccessioniPrimary (citable) accession number: P12938
Secondary accession number(s): O35106, Q5FVU3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: December 15, 1998
Last modified: October 29, 2014
This is version 120 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3