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P12938 (CP2D3_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 110. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cytochrome P450 2D3
EC=1.14.14.1
Alternative name(s):
CYPIID3
Cytochrome P450-DB3
Debrisoquine 4-hydroxylase
Gene names
Name:Cyp2d3
Synonyms:Cyp2d-3
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length500 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.

Catalytic activity

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactor

Heme group By similarity.

Subcellular location

Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.

Induction

P450 can be induced to high levels in liver and other tissues by various foreign compounds, including drugs, pesticides, and carcinogens.

Sequence similarities

Belongs to the cytochrome P450 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 500500Cytochrome P450 2D3
PRO_0000051729

Sites

Metal binding4461Iron (heme axial ligand)

Experimental info

Sequence conflict125 – 1262AP → CT in AAA41002. Ref.1
Sequence conflict125 – 1262AP → CT in CAA36270. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P12938 [UniParc].

Last modified December 15, 1998. Version 2.
Checksum: C54727C2C00F73F6

FASTA50056,642
        10         20         30         40         50         60 
MELLAGTGLW PMAIFTVIFI LLVDLMHRRQ RWTSRYPPGP VPWPVLGNLL QVDLCNMPYS 

        70         80         90        100        110        120 
MYKLQNRYGD VFSLQMGWKP VVVINGLKAV QELLVTCGED TADRPEMPIF QHIGYGHKAK 

       130        140        150        160        170        180 
GVVLAPYGPE WREQRRFSVS TLRNFGVGKK SLEQWVTDEA SHLCDALTAE AGRPLDPYTL 

       190        200        210        220        230        240 
LNKAVCNVIA SLIYARRFDY GDPDFIKVLK ILKESMGEQT GLFPEVLNMF PVLLRIPGLA 

       250        260        270        280        290        300 
DKVFPGQKTF LTMVDNLVTE HKKTWDPDQP PRDLTDAFLA EIEKAKGNPE SSFNDANLRL 

       310        320        330        340        350        360 
VVNDLFGAGM VTTSITLTWA LLLMILHPDV QCRVQQEIDE VIGQVRHPEM ADQAHMPFTN 

       370        380        390        400        410        420 
AVIHEVQRFA DIVPMNLPHK TSRDIEVQGF LIPKGTTLIP NLSSVLKDET VWEKPLRFHP 

       430        440        450        460        470        480 
EHFLDAQGNF VKHEAFMPFS AGRRACLGEP LARMELFLFF TCLLQRFSFS VPTGQPRPSD 

       490        500 
YGVFAFLLSP SPYQLCAFKR

« Hide

References

« Hide 'large scale' references
[1]"The CYP2D gene subfamily: analysis of the molecular basis of the debrisoquine 4-hydroxylase deficiency in DA rats."
Matsunaga E., Zanger U.M., Hardwick J.P., Gelboin H.V., Meyer U.A., Gonzalez F.J.
Biochemistry 28:7349-7355(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The rat P450 IID subfamily: complete sequences of four closely linked genes and evidence that gene conversions maintained sequence homogeneity at the heme-binding region of the cytochrome P450 active site."
Matsunaga E., Umeno M., Gonzalez F.J.
J. Mol. Evol. 30:155-169(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Sprague-Dawley.
Tissue: Liver.
[3]"Expression of four rat CYP2D isoforms in Saccharomyces cerevisiae and their catalytic specificity."
Wan J., Imaoka S., Chow T., Hiroi T., Yabusaki Y., Funae Y.
Arch. Biochem. Biophys. 348:383-390(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Sprague-Dawley.
Tissue: Liver.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		J02868 mRNA. Translation: AAA41002.1.
X52028 Genomic DNA. Translation: CAA36270.1.
AB008424 mRNA. Translation: BAA23124.1.
BC089769 mRNA. Translation: AAH89769.1.
IPIIPI00202580.
PIRS16872.
RefSeqNP_775116.1. NM_173093.1.
UniGeneRn.32106.

3D structure databases

ProteinModelPortalP12938.
SMRP12938. Positions 37-500.
ModBaseSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000048711.

PTM databases

PhosphoSiteP12938.

Proteomic databases

PaxDbP12938.
PRIDEP12938.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID24303.
KEGGrno:24303.
UCSCRGD:2472. rat.

Organism-specific databases

CTD24303.
RGD2472. Cyp2d3.

Phylogenomic databases

eggNOGCOG2124.
HOGENOMHOG000036991.
HOVERGENHBG015789.
InParanoidP12938.
KOK07414.
OrthoDBEOG40VVPH.

Enzyme and pathway databases

SABIO-RKP12938.

Gene expression databases

ArrayExpressP12938.
GenevestigatorP12938.
GermOnlineENSRNOG00000029179. Rattus norvegicus.

Family and domain databases

Gene3D1.10.630.10. 1 hit.
InterProIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008069. Cyt_P450_E_grp-I_CYP2D-like.
[Graphical view]
PfamPF00067. p450. 1 hit.
[Graphical view]
PRINTSPR00463. EP450I.
PR01686. EP450ICYP2D.
PR00385. P450.
SUPFAMSSF48264. Cytochrome_P450. 1 hit.
PROSITEPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

BindingDBP12938.
ChEMBLCHEMBL3057.
NextBio602923.

Entry information

Entry nameCP2D3_RAT
AccessionPrimary (citable) accession number: P12938
Secondary accession number(s): O35106, Q5FVU3
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: December 15, 1998
Last modified: April 3, 2013
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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