P12930 (CAPSD_PAVCN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Capsid protein VP1 Alternative name(s): Coat protein VP1
Organism	Canine parvovirus type 2 (isolate Dog/United States/CPV-N/1978) (CPV-2) [Complete proteome]
Taxonomic identifier	10791 [NCBI]
Taxonomic lineage	Viruses › ssDNA viruses › Parvoviridae › Parvovirinae › Parvovirus
Virus host	Canis familiaris (Dog) (Canis lupus familiaris) [TaxID: 9615] Felis catus (Cat) (Felis silvestris catus) [TaxID: 9685]

Protein attributes

Sequence length	727 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Capsid protein self-assembles to form an icosahedral capsid with a T=1 symmetry, about 22 nm in diameter, and consisting of 60 copies of two size variants of the capsid proteins, VP1 and VP2, which differ by the presence of an N-terminal extension in the minor protein VP1. The capsid encapsulates the genomic ssDNA. Capsid proteins are responsible for the attachment to host cell receptor TFRC. This attachment induces virion internalization predominantly through clathrin-endocytosis. Binding to the host receptors also induces capsid rearrangements leading to surface exposure of VP1 N-terminus, specifically its phospholipase A2-like region and nuclear localization signal(s). VP1 N-terminus might serve as a lipolytic enzyme to breach the endosomal membrane during entry into host cell. Intracytoplasmic transport involves microtubules and interaction between capsid proteins and host dynein. Exposure of nuclear localization signal probably allows nuclear import of capsids By similarity.
Subunit structure	Interacts with host TFRC By similarity.
Subcellular location	Virion By similarity. Host nucleus Potential.
Domain	The N-terminus of VP1 is sequestered within the mature capsid. It contains a phospholipase A2-like region and nuclear localization signals that might be exposed by capsid modifications during virus entry By similarity.
Miscellaneous	The capsids of autonomous parvoviruses expose a proportion of VP2 N-terminus and part of that sequence can be cleaved of to form VP3 By similarity.
Sequence similarities	Belongs to the parvoviridae capsid protein family.
Sequence caution	The sequence AAA67460.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence AAA67461.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Clathrin-mediated endocytosis of virus by host Cytoplasmic active transport of viral material Host-virus interaction Initiation of viral infection Microtubule-dependent active transport of viral material Viral attachment to host cell Viral penetration into host cytoplasm Viral penetration into host nucleus Viral penetration via permeabilization of host organellar membrane Virus endocytosis by host
Cellular component	Host nucleus Virion
Coding sequence diversity	Alternative splicing
Ligand	Magnesium Metal-binding
Molecular function	Capsid protein T=1 icosahedral capsid protein
PTM	Disulfide bond
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	interspecies interaction between organisms Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	host cell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell viral capsid Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW structural molecule activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform VP1 (identifier: P12930-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: Minor splicing isoform.

Isoform VP2 (identifier: P12930-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-143: Missing.

Note: Major splicing isoform produced by deletion of the initiating AUG for VP1 and downstream translation of VP2.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 727	727	Capsid protein VP1		PRO_0000039429
Regions
	Region	19 – 64	46	Phospholipase A2-like By similarity
	Motif	4 – 13	10	Nuclear localization signal Potential
	Compositional bias	165 – 182	18	Gly-rich
Sites
	Metal binding	323	1	Magnesium 1 By similarity
Amino acid modifications
	Disulfide bond	633 ↔ 637		By similarity
Natural variations
	Alternative sequence	1 – 143	143	Missing in isoform VP2.		VSP_041139

Sequences

	Sequence		Length	Mass (Da)
	Isoform VP1 [UniParc]. Last modified May 31, 2011. Version 2. Checksum: 76EA7954CC4539FB	FASTA	727	80,392
	10 20 30 40 50 60 MAPPAKRARR GLVPPGYKYL GPGNSLDQGE PTNPSDAAAK EHDEAYAAYL RSGKNPYLYF 70 80 90 100 110 120 SPADQRFIDQ TKDAKDWGGK IGHYFFRAKK AIAPVLTDTP DHPSTSRPTK PTKRSKPPPH 130 140 150 160 170 180 IFINLAKKKK AGAGQVKRDN LAPMSDGAVQ PDGGQPAVRN ERATGSGNGS GGGGGGGSGG 190 200 210 220 230 240 VGISTGTFNN QTEFKFLENG WVEITANSSR LVHLNMPESE NYRRVVVNNM DKTAVNGNMA 250 260 270 280 290 300 LDDIHAQIVT PWSLVDANAW GVWFNPGDWQ LIVNTMSELH LVSFEQEIFN VVLKTVSESA 310 320 330 340 350 360 TQPPTKVYNN DLTASLMVAL DSNNTMPFTP AAMRSETLGF YPWKPTIPTP WRYYFQWDRT 370 380 390 400 410 420 LIPSHTGTSG TPTNIYHGTD PDDVQFYTIE NSVPVHLLRT GDEFATGTFF FDCKPCRLTH 430 440 450 460 470 480 TWQTNRALGL PPFLNSLPQS EGATNFGDIG VQQDKRRGVT QMGNTNYITE ATIMRPAEVG 490 500 510 520 530 540 YSAPYYSFEA STQGPFKTPI AAGRGGAQTY ENQAADGDPR YAFGRQHGQK TTTTGETPER 550 560 570 580 590 600 FTYIAHQDTG RYPEGDWIQN INFNLPVTND NVLLPTDPIG GKTGINYTNI FNTYGPLTAL 610 620 630 640 650 660 NNVPPVYPNG QIWDKEFDTD LKPRLHVNAP FVCQNNCPGQ LFVKVAPNLT NEYDPDASAN 670 680 690 700 710 720 MSRIVTYSDF WWKGKLVFKA KLRASHTWNP IQQMSINVDN QFNYVPSNIG GMKIVYEKSQ LAPRKLY
	Isoform VP2 [UniParc]. Checksum: C7FD6F7DE56B2A4A	FASTA	584	64,701
	10 20 30 40 50 60 MSDGAVQPDG GQPAVRNERA TGSGNGSGGG GGGGSGGVGI STGTFNNQTE FKFLENGWVE 70 80 90 100 110 120 ITANSSRLVH LNMPESENYR RVVVNNMDKT AVNGNMALDD IHAQIVTPWS LVDANAWGVW 130 140 150 160 170 180 FNPGDWQLIV NTMSELHLVS FEQEIFNVVL KTVSESATQP PTKVYNNDLT ASLMVALDSN 190 200 210 220 230 240 NTMPFTPAAM RSETLGFYPW KPTIPTPWRY YFQWDRTLIP SHTGTSGTPT NIYHGTDPDD 250 260 270 280 290 300 VQFYTIENSV PVHLLRTGDE FATGTFFFDC KPCRLTHTWQ TNRALGLPPF LNSLPQSEGA 310 320 330 340 350 360 TNFGDIGVQQ DKRRGVTQMG NTNYITEATI MRPAEVGYSA PYYSFEASTQ GPFKTPIAAG 370 380 390 400 410 420 RGGAQTYENQ AADGDPRYAF GRQHGQKTTT TGETPERFTY IAHQDTGRYP EGDWIQNINF 430 440 450 460 470 480 NLPVTNDNVL LPTDPIGGKT GINYTNIFNT YGPLTALNNV PPVYPNGQIW DKEFDTDLKP 490 500 510 520 530 540 RLHVNAPFVC QNNCPGQLFV KVAPNLTNEY DPDASANMSR IVTYSDFWWK GKLVFKAKLR 550 560 570 580 ASHTWNPIQQ MSINVDNQFN YVPSNIGGMK IVYEKSQLAP RKLY

References

[1]	"Nucleotide sequence and genome organization of canine parvovirus." Reed A.P., Jones E.V., Miller T.J. J. Virol. 62:266-276(1988) [PubMed: 2824850] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	M19296 Genomic DNA. Translation: AAA67460.1. Sequence problems. M19296 Genomic DNA. Translation: AAA67461.1. Sequence problems.
PIR	VCPVCP. B29962.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR016184. Capsid/spike_ssDNA_virus. IPR001403. Parvovirus_coat. IPR013607. Parvovirus_coat_VP1_N. [Graphical view]
Gene3D	G3DSA:2.170.30.10. Parvo_coat. 1 hit.
Pfam	PF00740. Parvo_coat. 1 hit. PF08398. Parvo_coat_N. 1 hit. [Graphical view]
SUPFAM	SSF88645. Capsid/spike_ssDNA_virus. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CAPSD_PAVCN

Accession

Primary (citable) accession number: P12930
Secondary accession number(s): Q84387

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	May 31, 2011
Last modified:	September 21, 2011
This is version 58 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program