Reviewed, UniProtKB/Swiss-Prot P12928 (KPYR_RAT)

Names and origin

Protein names

Recommended name:
    Pyruvate kinase isozymes R/L
    EC=2.7.1.40
Alternative name(s):
    L-PK

Gene names

Name:

Pklr

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	574 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + pyruvate = ADP + phosphoenolpyruvate.
Cofactor	Magnesium. Potassium.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.
Subunit structure	Homotetramer.
Miscellaneous	There are 4 isozymes of pyruvate kinase in mammals: L, R, M1 and M2. L type is major isozyme in the liver, R is found in red cells, M1 is the main form in muscle, heart and brain, and M2 is found in early fetal tissues.
Sequence similarities	Belongs to the pyruvate kinase family.

Ontologies

Keywords
Biological process	Glycolysis
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding Pyruvate
Molecular function	Kinase Transferase
PTM	Acetylation Phosphoprotein
Gene Ontology (GO)
Biological process	ATP biosynthetic process Inferred from direct assay. Source: RGD cellular response to insulin stimulus Inferred from direct assay. Source: RGD glycolysis Inferred from direct assay. Source: RGD pyruvate biosynthetic process Inferred from direct assay. Source: RGD response to ATP Inferred from direct assay. Source: RGD response to cAMP Inferred from direct assay. Source: RGD response to glucose stimulus Inferred from expression pattern. Source: RGD response to heat Inferred from direct assay. Source: RGD response to hypoxia Inferred from expression pattern. Source: RGD response to lithium ion Inferred from direct assay. Source: RGD response to nutrient Inferred from expression pattern. Source: RGD response to organic cyclic substance Inferred from expression pattern. Source: RGD
Cellular component	cytosol Inferred from direct assay. Source: RGD soluble fraction Inferred from direct assay. Source: RGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW potassium ion binding Inferred from electronic annotation. Source: InterPro pyruvate kinase activity Inferred from direct assay. Source: RGD
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform R-type (identifier: P12928-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform L-type (identifier: P12928-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-33: MSVQENTLPQQLWPWIFRSQKDLAKSALSGAPG → ME

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 574	574	Pyruvate kinase isozymes R/L		PRO_0000112096
Sites
	Active site	313	1	By similarity
	Metal binding	315	1	Magnesium Potential
	Metal binding	336	1	Magnesium Potential
	Metal binding	337	1	Magnesium Potential
Amino acid modifications
	Modified residue	43	1	Phosphoserine By similarity
	Modified residue	348	1	N6-acetyllysine By similarity
	Modified residue	556	1	Phosphothreonine By similarity
Natural variations
	Alternative sequence	1 – 33	33	MSVQE…SGAPG → ME in isoform L-type.		VSP_002884
Experimental info
	Sequence conflict	130	1	S → Y in AAA41882. Ref.1
	Sequence conflict	130	1	S → Y in AAA41883. Ref.1
	Sequence conflict	322	1	K → R in AAA41882. Ref.1
	Sequence conflict	322	1	K → R in AAA41883. Ref.1
	Sequence conflict	498	1	R → G in AAA41880. Ref.3
	Sequence conflict	501	1	Q → K in AAA41880. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform R-type [UniParc]. Last modified February 1, 1995. Version 2. Checksum: 6BBC544653C51380	FASTA	574	62,200
	10 20 30 40 50 60 MSVQENTLPQ QLWPWIFRSQ KDLAKSALSG APGGPAGYLR RASVAQLTQE LGTAFFQQQQ 70 80 90 100 110 120 LPAAMADTFL EHLCLLDIDS QPVAARSTSI IATIGPASRS VDRLKEMIKA GMNIARLNFS 130 140 150 160 170 180 HGSHEYHAES IANIREATES FATSPLSYRP VAIALDTKGP EIRTGVLQGG PESEVEIVKG 190 200 210 220 230 240 SQVLVTVDPK FQTRGDAKTV WVDYHNITRV VAVGGRIYID DGLISLVVQK IGPEGLVTEV 250 260 270 280 290 300 EHGGILGSRK GVNLPNTEVD LPGLSEQDLL DLRFGVQHNV DIIFASFVRK ASDVLAVRDA 310 320 330 340 350 360 LGPEGQNIKI ISKIENHEGV KKFDEILEVS DGIMVARGDL GIEIPAEKVF LAQKMMIGRC 370 380 390 400 410 420 NLAGKPVVCA TQMLESMITK ARPTRAETSD VANAVLDGAD CIMLSGETAK GSFPVEAVMM 430 440 450 460 470 480 QHAIAREAEA AVYHRQLFEE LRRAAPLSRD PTEVTAIGAV EASFKCCAAA IIVLTKTGRS 490 500 510 520 530 540 AQLLSQYRPR AAVIAVTRSA QAARQVHLSR GVFPLLYREP PEAIWADDVD RRVQFGIESG 550 560 570 KLRGFLRVGD LVIVVTGWRP GSGYTNIMRV LSVS
	Isoform L-type. Checksum: E5472B97649E2A47	FASTA	543	58,794
	10 20 30 40 50 60 MEGPAGYLRR ASVAQLTQEL GTAFFQQQQL PAAMADTFLE HLCLLDIDSQ PVAARSTSII 70 80 90 100 110 120 ATIGPASRSV DRLKEMIKAG MNIARLNFSH GSHEYHAESI ANIREATESF ATSPLSYRPV 130 140 150 160 170 180 AIALDTKGPE IRTGVLQGGP ESEVEIVKGS QVLVTVDPKF QTRGDAKTVW VDYHNITRVV 190 200 210 220 230 240 AVGGRIYIDD GLISLVVQKI GPEGLVTEVE HGGILGSRKG VNLPNTEVDL PGLSEQDLLD 250 260 270 280 290 300 LRFGVQHNVD IIFASFVRKA SDVLAVRDAL GPEGQNIKII SKIENHEGVK KFDEILEVSD 310 320 330 340 350 360 GIMVARGDLG IEIPAEKVFL AQKMMIGRCN LAGKPVVCAT QMLESMITKA RPTRAETSDV 370 380 390 400 410 420 ANAVLDGADC IMLSGETAKG SFPVEAVMMQ HAIAREAEAA VYHRQLFEEL RRAAPLSRDP 430 440 450 460 470 480 TEVTAIGAVE ASFKCCAAAI IVLTKTGRSA QLLSQYRPRA AVIAVTRSAQ AARQVHLSRG 490 500 510 520 530 540 VFPLLYREPP EAIWADDVDR RVQFGIESGK LRGFLRVGDL VIVVTGWRPG SGYTNIMRVL SVS

References

[1]	"The L- and R-type isozymes of rat pyruvate kinase are produced from a single gene by use of different promoters." Noguchi T., Yamada K., Inoue H., Matsuda T., Tanaka T. J. Biol. Chem. 262:14366-14371(1987) [PubMed: 3654663] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete amino acid sequence of rat L-type pyruvate kinase deduced from the cDNA sequence." Inoue H., Noguchi T., Tanaka T. Eur. J. Biochem. 154:465-469(1986) [PubMed: 3002799] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Complete nucleotide and deduced amino acid sequences of rat L-type pyruvate kinase." Lone Y.-C., Simon M.-P., Kahn A., Marie J. FEBS Lett. 195:97-100(1986) [PubMed: 3080337] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]	"Structure of the rat L-type pyruvate kinase gene." Cognet M., Lone Y.C., Vaulont S., Kahn A., Marie J. J. Mol. Biol. 196:11-25(1987) [PubMed: 3309348] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M17091 , M17088, M17089, M17090 Genomic DNA. Translation: AAA41882.1. M17091 , M17088, M17089, M17090 Genomic DNA. Translation: AAA41883.1. M17685 mRNA. Translation: AAA41881.1. X05684 Genomic DNA. Translation: CAA29169.1. M11709 mRNA. Translation: AAA41880.1.
IPI	IPI00202549. IPI00231683.
PIR	KIRTPR. A27427. KIRTPL. A92940.
RefSeq	NP_036756.3.
UniGene	Rn.48821
3D structure databases
HSSP	HSSP built from PDB template 1LIU based on UniProtKB P30613.
ModBase	Search...
Protein-protein interaction databases
STRING	P12928.
PTM databases
PhosphoSite	P12928.
Proteomic databases
PRIDE	P12928.
Genome annotation databases
Ensembl	ENSRNOT00000027700; ENSRNOP00000027700; ENSRNOG00000020420; Rattus norvegicus. [Genome view]
GeneID	24651.
KEGG	rno:24651.
NMPDR	fig|10116.3.peg.16404.
UCSC	NM_012624. rat.
Organism-specific databases
RGD	3336. Pklr.
Phylogenomic databases
HOVERGEN	P12928.
OMA	WVSKSQR.
Enzyme and pathway databases
BRENDA	2.7.1.40. 248.
Gene expression databases
ArrayExpress	P12928.
Genevestigator	P12928.
GermOnline	ENSRNOG00000020420. Rattus norvegicus.
Family and domain databases
InterPro	IPR001697. Pyr_Knase. IPR015813. Pyrv/PenolPyrv_Kinase_cat. IPR015794. Pyrv_Knase_a/b. IPR018209. Pyrv_Knase_AS. IPR015793. Pyrv_Knase_brl. [Graphical view]
Gene3D	G3DSA:3.20.20.60. Pyrv/PenolPyrv_Kinase_cat. 1 hit. G3DSA:3.40.1380.20. Pyrv_Knase_a/b. 1 hit.
PANTHER	PTHR11817. Pyruvate_kinase. 1 hit.
Pfam	PF00224. PK. 1 hit. PF02887. PK_C. 1 hit. [Graphical view]
PRINTS	PR01050. PYRUVTKNASE.
ProDom	PD001009. Pyruvate_kinase. 2 hits. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01064. pyruv_kin. 1 hit.
PROSITE	PS00110. PYRUVATE_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KPYR_RAT

Accession

Primary (citable) accession number: P12928
Secondary accession number(s): P04763, Q64618

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	February 1, 1995
Last modified:	November 3, 2009
This is version 97 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)