P12918 (RDRP_IBDVA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RNA-directed RNA polymerase
Short name=RDRP
EC=2.7.7.48

Alternative name(s):
Protein VP1

Gene names

Name:

VP1

Organism

Avian infectious bursal disease virus (strain Australian 002-73) (IBDV) (Gumboro disease virus)

Taxonomic identifier

10997 [NCBI]

Taxonomic lineage

Viruses › dsRNA viruses › Birnaviridae › Avibirnavirus › Avian infectious bursal disease virus

Virus host

Gallus gallus (Chicken) [TaxID: 9031]
Meleagris gallopavo (Common turkey) [TaxID: 9103]

Protein attributes

Sequence length	878 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	RNA-dependent RNA polymerase which is found both free and covalently attached to the genomic RNA. May also contain guanylyl and methyl transferase activities By similarity.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Subunit structure	Interacts with VP3 in the cytoplasm By similarity.
Subcellular location	Virion By similarity. Note: Minor amounts are incorporated in the virion By similarity.
Post-translational modification	May exist in multiple phosphorylated forms.
Sequence similarities	Contains 1 RdRp catalytic domain.

Ontologies

Keywords
Biological process	Viral RNA replication
Cellular component	Virion
Ligand	GTP-binding Nucleotide-binding
Molecular function	Nucleotidyltransferase RNA-directed RNA polymerase Transferase
PTM	Covalent protein-RNA linkage Phosphoprotein
Gene Ontology (GO)
Biological_process	RNA-protein covalent cross-linking Inferred from electronic annotation. Source: UniProtKB-KW viral genome replication Inferred from electronic annotation. Source: InterPro
Cellular_component	virion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 878	878	RNA-directed RNA polymerase		PRO_0000221962
Regions
	Domain	397 – 597	201	RdRp catalytic
	Nucleotide binding	258 – 265	8	GTP Potential

Sequences

	Sequence		Length	Mass (Da)
	P12918 [UniParc]. Last modified October 1, 1989. Version 1. Checksum: 6B67C1E0AC193D1F	FASTA	878	97,274
	10 20 30 40 50 60 MSDVFNSPQA RTKISAAFGI KPTAGQDVEE LLIPKVWVPP EDPLASPSRL AKFLRENGYK 70 80 90 100 110 120 ILQPRSLPEN EEYETDQILP DLAWMRQIEG AVLKPTLSLH WGPRVLPKVL LNSPPEQGKA 130 140 150 160 170 180 QCVPTRHCTT QADIYLFLQV PEATESLKDE VTLLTQNIRD KAYGSGTYMG QATRLVAMKE 190 200 210 220 230 240 VATGRNPNKD PLKLGYTFES IAQLLDITLP VGPPGEDDKP WVPLTRVPSR MLVLTGDVDG 250 260 270 280 290 300 DFEVEDYLPK INLKSSSGLP YVGRTKGETI GEMIAISNQF LRELSALLKQ GAGTKGSNKK 310 320 330 340 350 360 KLLSMLSDYW YLSCGLLFPK AERYDKSTWL TKTRNIWSAP SPTHLMISMI TWPVMSNSPN 370 380 390 400 410 420 NVLNIEGCPS LYKFNPFRGG LNRIVEWILA PEEPKALVYA DNIYIVHSNT WYSIDLEKGE 430 440 450 460 470 480 ANCTRQHMQA AMYYILTRGW SDNGDPMFNQ TWASFAMNIA PALVVDSSCL IMNLQIKSYG 490 500 510 520 530 540 QGSGNAATFI NNHLLSTLVL DQWNLMKQPN PDSEEFKSIE DKLGINFKIE RSIDDIRGKL 550 560 570 580 590 600 RQLVPLAQPG YLSGGVEPEQ SSPTVELDLL GWSATYSKDL GIYVPVLDKE RLFCSAAYPK 610 620 630 640 650 660 GVENKSLKSK VGIEQAYKVV RYEALRLVGG WNYPLLNKAC KNNASAARRH LEAKGFPLDE 670 680 690 700 710 720 FLAEWSELSE FGETFEGFNI KLTVTRENLA ELNKPVPPKP PNVNRPVNTG GLKAVSNALK 730 740 750 760 770 780 TGRYRNEAGL SGLVLLATAR SRLQDAVKAK AEAEKLHKSK PDDPDADWFE RSETLSDLLE 790 800 810 820 830 840 KADVASKVAH SALVETSDAL EAVQSSSVYT PKYPEVKNPQ TASNPVVGLH LPAKRATGVQ 850 860 870 AALLGAGTSR PMGMEAPTRS KNAVKMAKRA QRQKESRQ

References

[1]	"Sequence of the small double-stranded RNA genomic segment of infectious bursal disease virus and its deduced 90-kDa product." Morgan M.M., Macreadie I.G., Harley V.R., Hudson P.J., Azad A.A. Virology 163:240-242(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	M19336 Genomic RNA. Translation: AAA89177.1.
PIR	RRXSIB. A28649.
3D structure databases
ProteinModelPortal	P12918.
SMR	P12918. Positions 31-803.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	1.10.287.540. 1 hit.
InterPro	IPR007100. RNA-dir_pol_birnavirus. IPR007098. RNA-dir_pol_mononegavirus. IPR023218. UPF0291_struct_dom. [Graphical view]
Pfam	PF04197. Birna_RdRp. 1 hit. [Graphical view]
PROSITE	PS50524. RDRP_DSRNA_BIR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RDRP_IBDVA

Accession

Primary (citable) accession number: P12918

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	October 1, 1989
Last modified:	April 3, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program