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Protein

Large envelope protein

Gene

S

Organism
Woodchuck hepatitis B virus (isolate 59) (WHV)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

The large envelope protein exists in two topological conformations, one which is termed 'external' or Le-HBsAg and the other 'internal' or Li-HBsAg. In its external conformation the protein attaches the virus to cell receptors and thereby initiating infection. This interaction determines the species specificity and liver tropism. This attachment induces virion internalization predominantly through caveolin-mediated endocytosis. The large envelope protein also assures fusion between virion membrane and endosomal membrane. In its internal conformation the protein plays a role in virion morphogenesis and mediates the contact with the nucleocapsid like a matrix protein.UniRule annotation
The middle envelope protein plays an important role in the budding of the virion. It is involved in the induction of budding in a nucleocapsid independent way. In this process the majority of envelope proteins bud to form subviral lipoprotein particles of 22 nm of diameter that do not contain a nucleocapsid.UniRule annotation

GO - Biological processi

Keywordsi

Biological processCaveolin-mediated endocytosis of virus by host, Fusion of virus membrane with host endosomal membrane, Fusion of virus membrane with host membrane, Host-virus interaction, Viral attachment to host cell, Viral penetration into host cytoplasm, Virus endocytosis by host, Virus entry into host cell

Names & Taxonomyi

Protein namesi
Recommended name:
Large envelope proteinUniRule annotation
Alternative name(s):
L glycoproteinUniRule annotation
L-HBsAgUniRule annotation
Short name:
LHBUniRule annotation
Large S proteinUniRule annotation
Large surface proteinUniRule annotation
Major surface antigenUniRule annotation
Gene namesi
Name:SUniRule annotation
OrganismiWoodchuck hepatitis B virus (isolate 59) (WHV)
Taxonomic identifieri10431 [NCBI]
Taxonomic lineageiVirusesRetro-transcribing virusesHepadnaviridaeOrthohepadnavirus
Virus hostiMarmota monax (Woodchuck) [TaxID: 9995]
Proteomesi
  • UP000007542 Componenti: Genome

Subcellular locationi

  • Virion membrane UniRule annotation

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini2 – 286Intravirion; in internal conformationUniRule annotationAdd BLAST285
Topological domaini2 – 214Virion surface; in external conformationUniRule annotationAdd BLAST213
Transmembranei215 – 235Helical; Name=TM1; Note=In external conformationUniRule annotationAdd BLAST21
Topological domaini236 – 286Intravirion; in external conformationUniRule annotationAdd BLAST51
Transmembranei287 – 307Helical; Name=TM2UniRule annotationAdd BLAST21
Topological domaini308 – 379Virion surfaceUniRule annotationAdd BLAST72
Transmembranei380 – 400HelicalUniRule annotationAdd BLAST21
Topological domaini401 – 406IntravirionUniRule annotation6
Transmembranei407 – 429Helical; Name=TM3UniRule annotationAdd BLAST23
Topological domaini430 – 431Virion surfaceUniRule annotation2

GO - Cellular componenti

Keywords - Cellular componenti

Membrane, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved; by hostUniRule annotation
ChainiPRO_00000381232 – 431Large envelope proteinUniRule annotationAdd BLAST430

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycine; by hostUniRule annotation1
Glycosylationi351N-linked (GlcNAc...) asparagine; by hostUniRule annotation1
Isoform M (identifier: P12910-2)
Glycosylationi3N-linked (GlcNAc...) asparagineBy similarity1

Post-translational modificationi

Isoform M is N-terminally acetylated by host at a ratio of 90%, and N-glycosylated by host at the pre-S2 region.UniRule annotation
Myristoylated.UniRule annotation

Keywords - PTMi

Acetylation, Glycoprotein, Lipoprotein, Myristate

Interactioni

Subunit structurei

Li-HBsAg interacts with capsid protein and with HDV Large delta antigen. Isoform M associates with host chaperone CANX through its pre-S2 N glycan. This association may be essential for M proper secretion.UniRule annotation

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 207Pre-SUniRule annotationAdd BLAST206
Regioni2 – 148Pre-S1UniRule annotationAdd BLAST147
Regioni149 – 207Pre-S2UniRule annotationAdd BLAST59

Domaini

The large envelope protein is synthesized with the pre-S region at the cytosolic side of the endoplasmic reticulum and, hence will be within the virion after budding. Therefore the pre-S region is not N-glycosylated. Later a post-translational translocation of N-terminal pre-S and TM1 domains occur in about 50% of proteins at the virion surface. These molecules change their topology by an unknown mechanism, resulting in exposure of pre-S region at virion surface. For isoform M in contrast, the pre-S2 region is translocated cotranslationally to the endoplasmic reticulum lumen and is N-glycosylated.UniRule annotation

Sequence similaritiesi

Belongs to the orthohepadnavirus major surface antigen family.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

OrthoDBiVOG090000AH.

Family and domain databases

HAMAPiMF_04075. HBV_HBSAG. 1 hit.
InterProiView protein in InterPro
IPR000349. Hepvir_surfAg.
PfamiView protein in Pfam
PF00695. vMSA. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing and alternative initiation. AlignAdd to basket

Isoform L (identifier: P12910-1) [UniParc]FASTAAdd to basket
Also known as: Large envelope protein, LHB, L-HBsAg

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGNNIKVTFN PDKIAAWWPA VGTYYTTTYP QNQSVFQPGI YQTTSLINPK
60 70 80 90 100
NQQELDSVLI NRYKQIDWNT WQGFPVDQKL PLVSRDPPLK PHINQSAQTF
110 120 130 140 150
EIKPGPIIVP GIRDIPRGLV PPQTPTNRDQ GRKPTPPTPP LRDTHPHLTM
160 170 180 190 200
KNQTFRLQGF VDGLRDLTTT ERYHNAYGDP FTTLSPVVPT VSTILSPPST
210 220 230 240 250
TGDPALSPEM SPSSLLGLLA GLQVVYFLWT KILTIAQNLD WWWTSLSFPG
260 270 280 290 300
GIPECTGQNS QFQTCKHLPT SCPPTCNGFR WMYLRRFIIY LLVLLLCLIF
310 320 330 340 350
LLVLLDWKGL IPVCPLQPTT ETTVNCRQCT LSVQDTYTPP YCCCLKPTAG
360 370 380 390 400
NCTCWPIPSS WALGNYLWEW ALARFSWLNL LVPLLQWLGG ISLIAWFLLI
410 420 430
WMIWFWGPAL LSILPPFIPI FVLFFLIWVY I
Length:431
Mass (Da):49,018
Last modified:October 1, 1989 - v1
Checksum:i5D117A1129CB0362
GO
Isoform M (identifier: P12910-2) [UniParc]FASTAAdd to basket
Also known as: Middle envelope protein, MHB, M-HBsAg

The sequence of this isoform differs from the canonical sequence as follows:
     1-149: Missing.

Show »
Length:282
Mass (Da):32,126
Checksum:iEE63DE186BE403EA
GO
Isoform S (identifier: P12910-3) [UniParc]FASTAAdd to basket
Also known as: Small envelope protein, SHB, S-HBsAg

The sequence of this isoform differs from the canonical sequence as follows:
     1-209: Missing.

Show »
Length:222
Mass (Da):25,605
Checksum:i79EF9B3085F605F3
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0314601 – 209Missing in isoform S. CuratedAdd BLAST209
Alternative sequenceiVSP_0314611 – 149Missing in isoform M. CuratedAdd BLAST149

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M19183 Genomic DNA. Translation: AAA46762.1.
PIRiH29969. SAVL59.

Keywords - Coding sequence diversityi

Alternative initiation, Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiHBSAG_WHV3
AccessioniPrimary (citable) accession number: P12910
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 1, 1989
Last modified: August 30, 2017
This is version 61 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families