P12904 (SNF4_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nuclear protein SNF4

Alternative name(s):
Regulatory protein CAT3

Gene names

Name:	SNF4
Synonyms:	CAT3
Ordered Locus Names:	YGL115W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	322 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This protein causes expression of glucose-repressible genes upon glucose deprivation. It interacts and has functional relationship to the protein-kinase SNF1.
Subcellular location	Nucleus.
Miscellaneous	Present with 11700 molecules/cell in log phase SD medium. Ref.7
Sequence similarities	Belongs to the 5'-AMP-activated protein kinase gamma subunit family. Contains 4 CBS domains.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Transcription Transcription regulation
Cellular component	Nucleus
Domain	CBS domain Repeat
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: UniProtKB-KW peroxisome organization Inferred from mutant phenotype. Source: SGD positive regulation of gluconeogenesis Inferred from mutant phenotype Ref.2. Source: SGD protein phosphorylation Inferred from mutant phenotype. Source: SGD regulation of transcription from RNA polymerase II promoter Inferred from genetic interaction. Source: SGD replicative cell aging Inferred from mutant phenotype. Source: SGD transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	AMP-activated protein kinase complex Inferred from direct assay Ref.2. Source: SGD cytoplasm Inferred from direct assay Ref.2. Source: SGD nuclear envelope lumen Inferred from direct assay. Source: SGD plasma membrane Inferred from direct assay. Source: SGD
Molecular function	catalytic activity Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct protein serine/threonine kinase activator activity Inferred from mutant phenotype. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
GAL83	Q04739	4	EBI-17537,EBI-7244
SIP1	P32578	5	EBI-17537,EBI-17179
SIP2	P34164	7	EBI-17537,EBI-17187
SNF1	P06782	17	EBI-17537,EBI-17516
YCL046W	P25575	3	EBI-17537,EBI-21748

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 322

322

Nuclear protein SNF4

PRO_0000204389

Regions

Domain

37 – 97

61

CBS 1

Domain

118 – 181

64

CBS 2

Domain

194 – 253

60

CBS 3

Domain

262 – 322

61

CBS 4

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

322

Helix Strand Turn

Details...

Helix

8 – 26

19

Helix

31 – 34

4

Beta strand

35 – 45

11

Helix

50 – 59

10

Beta strand

65 – 69

5

Turn

70 – 73

4

Beta strand

74 – 79

6

Helix

81 – 93

13

Helix

98 – 102

5

Helix

106 – 113

8

Beta strand

115 – 117

3

Helix

132 – 139

8

Beta strand

146 – 150

5

Beta strand

161 – 166

6

Helix

167 – 177

11

Helix

182 – 185

4

Helix

188 – 190

3

Helix

208 – 218

11

Beta strand

221 – 226

6

Beta strand

231 – 237

7

Helix

238 – 246

9

Helix

257 – 263

7

Helix

280 – 290

11

Beta strand

293 – 298

6

Beta strand

302 – 309

8

Helix

310 – 319

10

Sequences

	Sequence		Length	Mass (Da)
	P12904 [UniParc]. Last modified October 1, 1989. Version 1. Checksum: 51B387E346EE9561	FASTA	322	36,401
	10 20 30 40 50 60 MKPTQDSQEK VSIEQQLAVE SIRKFLNSKT SYDVLPVSYR LIVLDTSLLV KKSLNVLLQN 70 80 90 100 110 120 SIVSAPLWDS KTSRFAGLLT TTDFINVIQY YFSNPDKFEL VDKLQLDGLK DIERALGVDQ 130 140 150 160 170 180 LDTASIHPSR PLFEACLKML ESRSGRIPLI DQDEETHREI VVSVLTQYRI LKFVALNCRE 190 200 210 220 230 240 THFLKIPIGD LNIITQDNMK SCQMTTPVID VIQMLTQGRV SSVPIIDENG YLINVYEAYD 250 260 270 280 290 300 VLGLIKGGIY NDLSLSVGEA LMRRSDDFEG VYTCTKNDKL STIMDNIRKA RVHRFFVVDD 310 320 VGRLVGVLTL SDILKYILLG SN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular characterization of yeast regulatory gene CAT3 necessary for glucose derepression and nuclear localization of its product." Schueller H.-J., Entian K.-D. Gene 67:247-257(1988) [PubMed: 3049255] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Molecular analysis of the SNF4 gene of Saccharomyces cerevisiae: evidence for physical association of the SNF4 protein with the SNF1 protein kinase." Celenza J.L., Eng F.J., Carlson M. Mol. Cell. Biol. 9:5045-5054(1989) [PubMed: 2481228] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Correct end of the ORF for the CDC20 gene of Saccharomyces cerevisiae." Doi A., Doi K. Submitted (JUN-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-21.
[6]	"Mammalian AMP-activated protein kinase shares structural and functional homology with the catalytic domain of yeast Snf1 protein kinase." Mitchelhill K.I., Stapleton D., Gao G., House C., Michell B., Katsis F., Witters L.A., Kemp B.E. J. Biol. Chem. 269:2361-2364(1994) [PubMed: 7905477] [Abstract] Cited for: PROTEIN SEQUENCE OF 30-34 AND 316-322.
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M21760 Genomic DNA. Translation: AAA34472.1.
M30470 Genomic DNA. Translation: AAA35061.1.
Z72637 Genomic DNA. Translation: CAA96823.1.
D16506 Genomic DNA. Translation: BAA03958.1.
BK006941 Genomic DNA. Translation: DAA07993.1.

PIR

RGBYC3. A38906.

RefSeq

NP_011400.1. NM_001180980.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2NYC	X-ray	1.90	A	179-322	[»]
2NYE	X-ray	2.50	A/B	179-322	[»]
2QLV	X-ray	2.60	C/F	7-321	[»]
3T4N	X-ray	2.30	C	2-322	[»]
3TDH	X-ray	2.30	C	2-322	[»]
3TE5	X-ray	2.50	C	2-322	[»]

ProteinModelPortal

P12904.

SMR

P12904. Positions 7-321.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-592N.

IntAct

P12904. 39 interactions.

MINT

MINT-364345.

STRING

P12904.

Proteomic databases

PeptideAtlas

P12904.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YGL115W; YGL115W; YGL115W.

GeneID

852763.

KEGG

sce:YGL115W.

NMPDR

fig|4932.3.peg.2505.

Organism-specific databases

CYGD

YGL115w.

SGD

S000003083. SNF4.

Phylogenomic databases

eggNOG

fuNOG07696.

GeneTree

EFGT00050000005694.

HOGENOM

HBG446629.

OMA

PVIDVIQ.

OrthoDB

EOG4SR15D.

Gene expression databases

ArrayExpress

P12904.

Genevestigator

P12904.

GermOnline

YGL115W. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR013785. Aldolase_TIM.
IPR000644. Cysta_beta_synth_core.
[Graphical view]

Gene3D

G3DSA:3.20.20.70. Aldolase_TIM. 2 hits.

Pfam

PF00571. CBS. 2 hits.
[Graphical view]

SMART

SM00116. CBS. 4 hits.
[Graphical view]

PROSITE

PS51371. CBS. 4 hits.
[Graphical view]

ProtoNet

Search...

Other

NextBio

972215.

Entry information

Entry name

SNF4_YEAST

Accession

Primary (citable) accession number: P12904
Secondary accession number(s): D6VU32

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	October 1, 1989
Last modified:	January 25, 2012
This is version 109 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program