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Protein

Uracil-DNA glycosylase

Gene

UNG1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine. Not involved in strand-directed mismatch repair.4 Publications

Miscellaneous

Present with 4820 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Hydrolyzes single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil.1 Publication

pH dependencei

Optimum pH is 7.5-8.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei162Proton acceptor1

GO - Molecular functioni

  • uracil DNA N-glycosylase activity Source: SGD

GO - Biological processi

  • base-excision repair, AP site formation via deaminated base removal Source: GO_Central
  • DNA repair Source: SGD

Keywordsi

Molecular functionHydrolase
Biological processDNA damage, DNA repair

Enzyme and pathway databases

BioCyciYEAST:G3O-32624-MONOMER.
ReactomeiR-SCE-110329. Cleavage of the damaged pyrimidine.
R-SCE-110357. Displacement of DNA glycosylase by APEX1.

Names & Taxonomyi

Protein namesi
Recommended name:
Uracil-DNA glycosylase (EC:3.2.2.27)
Short name:
UDG
Gene namesi
Name:UNG1
Ordered Locus Names:YML021C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YML021C.
SGDiS000004483. UNG1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 21MitochondrionSequence analysisAdd BLAST21
ChainiPRO_000003608522 – 359Uracil-DNA glycosylaseAdd BLAST338

Proteomic databases

MaxQBiP12887.
PRIDEiP12887.

Expressioni

Inductioni

Induced in late G1 and early S phase of the cell cycle.2 Publications

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
POL30P158734EBI-2097931,EBI-12993

Protein-protein interaction databases

BioGridi35148. 71 interactors.
DIPiDIP-8264N.
ELMiP12887.
IntActiP12887. 1 interactor.
MINTiMINT-4496681.
STRINGi4932.YML021C.

Structurei

3D structure databases

ProteinModelPortaliP12887.
SMRiP12887.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the uracil-DNA glycosylase (UDG) superfamily. UNG family.

Keywords - Domaini

Transit peptide

Phylogenomic databases

GeneTreeiENSGT00390000003405.
HOGENOMiHOG000229528.
InParanoidiP12887.
KOiK03648.
OMAiDNGYLMP.
OrthoDBiEOG092C45JB.

Family and domain databases

CDDicd10027. UDG_F1. 1 hit.
Gene3Di3.40.470.10. 1 hit.
HAMAPiMF_00148. UDG. 1 hit.
InterProiView protein in InterPro
IPR002043. UDG_fam1.
IPR018085. Ura-DNA_Glyclase_AS.
IPR005122. Uracil-DNA_glycosylase-like.
IPR036895. Uracil-DNA_glycosylase-like_sf.
PANTHERiPTHR11264. PTHR11264. 1 hit.
PfamiView protein in Pfam
PF03167. UDG. 1 hit.
SMARTiView protein in SMART
SM00986. UDG. 1 hit.
SUPFAMiSSF52141. SSF52141. 1 hit.
TIGRFAMsiTIGR00628. ung. 1 hit.
PROSITEiView protein in PROSITE
PS00130. U_DNA_GLYCOSYLASE. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P12887-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MWCMRRLPTN SVMTVARKRK QTTIEDFFGT KKSTNEAPNK KGKSGATFMT
60 70 80 90 100
ITNGAAIKTE TKAVAKEANT DKYPANSNAK DVYSKNLSSN LRTLLSLELE
110 120 130 140 150
TIDDSWFPHL MDEFKKPYFV KLKQFVTKEQ ADHTVFPPAK DIYSWTRLTP
160 170 180 190 200
FNKVKVVIIG QDPYHNFNQA HGLAFSVKPP TPAPPSLKNI YKELKQEYPD
210 220 230 240 250
FVEDNKVGDL THWASQGVLL LNTSLTVRAH NANSHSKHGW ETFTKRVVQL
260 270 280 290 300
LIQDREADGK SLVFLLWGNN AIKLVESLLG STSVGSGSKY PNIMVMKSVH
310 320 330 340 350
PSPLSASRGF FGTNHFKMIN DWLYNTRGEK MIDWSVVPGT SLREVQEANA

RLESESKDP
Length:359
Mass (Da):40,471
Last modified:October 1, 1989 - v1
Checksum:iCC06971E05FE7751
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti173L → S in AAT93039 (PubMed:17322287).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04470 Genomic DNA. Translation: AAA35195.1.
Z46659 Genomic DNA. Translation: CAA86634.1.
AY693020 Genomic DNA. Translation: AAT93039.1.
BK006946 Genomic DNA. Translation: DAA09877.1.
PIRiA31425.
RefSeqiNP_013691.1. NM_001182379.1.

Genome annotation databases

EnsemblFungiiYML021C; YML021C; YML021C.
GeneIDi854987.
KEGGisce:YML021C.

Similar proteinsi

Entry informationi

Entry nameiUNG_YEAST
AccessioniPrimary (citable) accession number: P12887
Secondary accession number(s): D6VZF3, E9P912
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 1, 1989
Last modified: November 22, 2017
This is version 150 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names