P12882 (MYH1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Myosin-1

Alternative name(s):
Myosin heavy chain 1
Myosin heavy chain 2x
Short name=MyHC-2x
Myosin heavy chain IIx/d
Short name=MyHC-IIx/d
Myosin heavy chain, skeletal muscle, adult 1

Gene names

Name:

MYH1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	1939 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Muscle contraction.
Subunit structure	Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2).
Subcellular location	Cytoplasm › myofibril. Note: Thick filaments of the myofibrils.
Domain	The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils. Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).
Sequence similarities	Contains 1 IQ domain. Contains 1 myosin head-like domain.
Caution	Represents a conventional myosin. This protein should not be confused with the unconventional myosin-1 (MYO1).

Ontologies

Keywords
Cellular component	Cytoplasm Thick filament
Coding sequence diversity	Polymorphism
Domain	Coiled coil
Ligand	ATP-binding Actin-binding Calmodulin-binding Nucleotide-binding
Molecular function	Motor protein Muscle protein Myosin
PTM	Methylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	A band Inferred from electronic annotation. Source: Compara focal adhesion Inferred from direct assay. Source: HPA intercalated disc Inferred from electronic annotation. Source: Compara muscle myosin complex Inferred from direct assay PubMed 18310078. Source: MGI myosin filament Inferred from electronic annotation. Source: UniProtKB-KW nucleolus Inferred from direct assay. Source: HPA
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW motor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1939	1939	Myosin-1		PRO_0000123391
Regions
	Domain	1 – 784	784	Myosin head-like
	Domain	785 – 814	30	IQ
	Nucleotide binding	179 – 186	8	ATP Potential
	Region	659 – 681	23	Actin-binding By similarity
	Region	761 – 775	15	Actin-binding By similarity
	Coiled coil	843 – 1939	1097	Potential
Amino acid modifications
	Modified residue	130	1	N6,N6,N6-trimethyllysine Potential
	Modified residue	1928	1	Phosphoserine By similarity
	Modified residue	1933	1	Phosphothreonine By similarity
Natural variations
	Natural variant	640	1	G → S. Ref.6		VAR_054159
	Natural variant	1306	1	S → L in a breast cancer sample; somatic mutation. Ref.5		VAR_036003
	Natural variant	1341	1	R → C. Corresponds to variant rs3744564 [ dbSNP | Ensembl ].		VAR_030193
	Natural variant	1445	1	A → T in a breast cancer sample; somatic mutation. Ref.5		VAR_036004
	Natural variant	1539	1	Q → H. Corresponds to variant rs3764850 [ dbSNP | Ensembl ].		VAR_030194
	Natural variant	1566	1	Q → K Found in a renal cell carcinoma sample; somatic mutation. Ref.7		VAR_064735
	Natural variant	1598	1	V → M in a breast cancer sample; somatic mutation. Ref.5		VAR_036005
	Natural variant	1716	1	R → C. Corresponds to variant rs1077841 [ dbSNP | Ensembl ].		VAR_030195
Experimental info
	Sequence conflict	1070	1	T → A in AAD29951. Ref.1
	Sequence conflict	1070	1	T → A in CAA27380. Ref.4
	Sequence conflict	1131	1	A → T in CAA27380. Ref.4
	Sequence conflict	1139	1	Q → L in CAA27380. Ref.4
	Sequence conflict	1158	1	G → V in CAA27380. Ref.4
	Sequence conflict	1163	1	A → T in CAA27380. Ref.4
	Sequence conflict	1286 – 1289	4	TESG → QNQV in CAA27380. Ref.4
	Sequence conflict	1302 – 1303	2	VS → ET in CAA27380. Ref.4
	Sequence conflict	1451	1	R → T in CAA27380. Ref.4
	Sequence conflict	1470	1	E → V in CAA27380. Ref.4
	Sequence conflict	1473 – 1474	2	AS → SF in CAA27380. Ref.4
	Sequence conflict	1531	1	E → K in AAI14546. Ref.3
	Sequence conflict	1569	1	L → V in CAA27380. Ref.4
	Sequence conflict	1598	1	V → E in CAA27380. Ref.4
	Sequence conflict	1606	1	D → N in CAA27380. Ref.4
	Sequence conflict	1643	1	A → D in CAA27380. Ref.4
	Sequence conflict	1648	1	R → Q in CAA27380. Ref.4
	Sequence conflict	1750	1	Q → K in CAA27380. Ref.4
	Sequence conflict	1822	1	R → K in CAA27380. Ref.4
	Sequence conflict	1845	1	R → H in CAA27380. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P12882 [UniParc]. Last modified April 14, 2009. Version 3. Checksum: D2B6F28C2C63CBDF	FASTA	1,939	223,145
	10 20 30 40 50 60 MSSDSEMAIF GEAAPFLRKS ERERIEAQNK PFDAKTSVFV VDPKESFVKA TVQSREGGKV 70 80 90 100 110 120 TAKTEAGATV TVKDDQVFPM NPPKYDKIED MAMMTHLHEP AVLYNLKERY AAWMIYTYSG 130 140 150 160 170 180 LFCVTVNPYK WLPVYNAEVV TAYRGKKRQE APPHIFSISD NAYQFMLTDR ENQSILITGE 190 200 210 220 230 240 SGAGKTVNTK RVIQYFATIA VTGEKKKEEV TSGKMQGTLE DQIISANPLL EAFGNAKTVR 250 260 270 280 290 300 NDNSSRFGKF IRIHFGTTGK LASADIETYL LEKSRVTFQL KAERSYHIFY QIMSNKKPDL 310 320 330 340 350 360 IEMLLITTNP YDYAFVSQGE ITVPSIDDQE ELMATDSAIE ILGFTSDERV SIYKLTGAVM 370 380 390 400 410 420 HYGNMKFKQK QREEQAEPDG TEVADKAAYL QNLNSADLLK ALCYPRVKVG NEYVTKGQTV 430 440 450 460 470 480 QQVYNAVGAL AKAVYDKMFL WMVTRINQQL DTKQPRQYFI GVLDIAGFEI FDFNSLEQLC 490 500 510 520 530 540 INFTNEKLQQ FFNHHMFVLE QEEYKKEGIE WTFIDFGMDL AACIELIEKP MGIFSILEEE 550 560 570 580 590 600 CMFPKATDTS FKNKLYEQHL GKSNNFQKPK PAKGKPEAHF SLIHYAGTVD YNIAGWLDKN 610 620 630 640 650 660 KDPLNETVVG LYQKSAMKTL ALLFVGATGA EAEAGGGKKG GKKKGSSFQT VSALFRENLN 670 680 690 700 710 720 KLMTNLRSTH PHFVRCIIPN ETKTPGAMEH ELVLHQLRCN GVLEGIRICR KGFPSRILYA 730 740 750 760 770 780 DFKQRYKVLN ASAIPEGQFI DSKKASEKLL GSIDIDHTQY KFGHTKVFFK AGLLGLLEEM 790 800 810 820 830 840 RDEKLAQLIT RTQAMCRGFL ARVEYQKMVE RRESIFCIQY NVRAFMNVKH WPWMKLYFKI 850 860 870 880 890 900 KPLLKSAETE KEMANMKEEF EKTKEELAKT EAKRKELEEK MVTLMQEKND LQLQVQAEAD 910 920 930 940 950 960 SLADAEERCD QLIKTKIQLE AKIKEVTERA EDEEEINAEL TAKKRKLEDE CSELKKDIDD 970 980 990 1000 1010 1020 LELTLAKVEK EKHATENKVK NLTEEMAGLD ETIAKLTKEK KALQEAHQQT LDDLQAEEDK 1030 1040 1050 1060 1070 1080 VNTLTKAKIK LEQQVDDLEG SLEQEKKIRM DLERAKRKLE GDLKLAQEST MDIENDKQQL 1090 1100 1110 1120 1130 1140 DEKLKKKEFE MSGLQSKIED EQALGMQLQK KIKELQARIE ELEEEIEAER ASRAKAEKQR 1150 1160 1170 1180 1190 1200 SDLSRELEEI SERLEEAGGA TSAQIEMNKK REAEFQKMRR DLEEATLQHE ATAATLRKKH 1210 1220 1230 1240 1250 1260 ADSVAELGEQ IDNLQRVKQK LEKEKSEMKM EIDDLASNME TVSKAKGNLE KMCRALEDQL 1270 1280 1290 1300 1310 1320 SEIKTKEEEQ QRLINDLTAQ RARLQTESGE YSRQLDEKDT LVSQLSRGKQ AFTQQIEELK 1330 1340 1350 1360 1370 1380 RQLEEEIKAK SALAHALQSS RHDCDLLREQ YEEEQEAKAE LQRAMSKANS EVAQWRTKYE 1390 1400 1410 1420 1430 1440 TDAIQRTEEL EEAKKKLAQR LQDAEEHVEA VNAKCASLEK TKQRLQNEVE DLMIDVERTN 1450 1460 1470 1480 1490 1500 AACAALDKKQ RNFDKILAEW KQKCEETHAE LEASQKESRS LSTELFKIKN AYEESLDQLE 1510 1520 1530 1540 1550 1560 TLKRENKNLQ QEISDLTEQI AEGGKRIHEL EKIKKQVEQE KSELQAALEE AEASLEHEEG 1570 1580 1590 1600 1610 1620 KILRIQLELN QVKSEVDRKI AEKDEEIDQM KRNHIRIVES MQSTLDAEIR SRNDAIRLKK 1630 1640 1650 1660 1670 1680 KMEGDLNEME IQLNHANRMA AEALRNYRNT QAILKDTQLH LDDALRSQED LKEQLAMVER 1690 1700 1710 1720 1730 1740 RANLLQAEIE ELRATLEQTE RSRKIAEQEL LDASERVQLL HTQNTSLINT KKKLETDISQ 1750 1760 1770 1780 1790 1800 IQGEMEDIIQ EARNAEEKAK KAITDAAMMA EELKKEQDTS AHLERMKKNL EQTVKDLQHR 1810 1820 1830 1840 1850 1860 LDEAEQLALK GGKKQIQKLE ARVRELEGEV ESEQKRNVEA VKGLRKHERK VKELTYQTEE 1870 1880 1890 1900 1910 1920 DRKNILRLQD LVDKLQAKVK SYKRQAEEAE EQSNVNLSKF RRIQHELEEA EERADIAESQ 1930 VNKLRVKSRE VHTKIISEE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Comparative sequence analysis of the complete human sarcomeric myosin heavy chain family: implications for functional diversity." Weiss A., Schiaffino S., Leinwand L.A. J. Mol. Biol. 290:61-75(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Skeletal muscle.
[2]	"DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage." Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L. , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C. Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"Characterization of diverse forms of myosin heavy chain expressed in adult human skeletal muscle." Saez L., Leinwand L.A. Nucleic Acids Res. 14:2951-2969(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1064-1939.
[5]	"The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E. Science 314:268-274(2006) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANTS [LARGE SCALE ANALYSIS] LEU-1306; THR-1445 AND MET-1598.
[6]	"DNA sequencing of a cytogenetically normal acute myeloid leukaemia genome." Ley T.J., Mardis E.R., Ding L., Fulton B., McLellan M.D., Chen K., Dooling D., Dunford-Shore B.H., McGrath S., Hickenbotham M., Cook L., Abbott R., Larson D.E., Koboldt D.C., Pohl C., Smith S., Hawkins A., Abbott S. , Locke D., Hillier L.W., Miner T., Fulton L., Magrini V., Wylie T., Glasscock J., Conyers J., Sander N., Shi X., Osborne J.R., Minx P., Gordon D., Chinwalla A., Zhao Y., Ries R.E., Payton J.E., Westervelt P., Tomasson M.H., Watson M., Baty J., Ivanovich J., Heath S., Shannon W.D., Nagarajan R., Walter M.J., Link D.C., Graubert T.A., DiPersio J.F., Wilson R.K. Nature 456:66-72(2008) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] SER-640.
[7]	"Exome sequencing identifies frequent mutation of the SWI/SNF complex gene PBRM1 in renal carcinoma." Varela I., Tarpey P., Raine K., Huang D., Ong C.K., Stephens P., Davies H., Jones D., Lin M.L., Teague J., Bignell G., Butler A., Cho J., Dalgliesh G.L., Galappaththige D., Greenman C., Hardy C., Jia M. , Latimer C., Lau K.W., Marshall J., McLaren S., Menzies A., Mudie L., Stebbings L., Largaespada D.A., Wessels L.F.A., Richard S., Kahnoski R.J., Anema J., Tuveson D.A., Perez-Mancera P.A., Mustonen V., Fischer A., Adams D.J., Rust A., Chan-On W., Subimerb C., Dykema K., Furge K., Campbell P.J., Teh B.T., Stratton M.R., Futreal P.A. Nature 469:539-542(2011) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANT LYS-1566.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF111785 mRNA. Translation: AAD29951.1. AC005323 Genomic DNA. No translation available. BC114545 mRNA. Translation: AAI14546.1. X03740 mRNA. Translation: CAA27380.1.
IPI	IPI00025879.
PIR	A23767.
RefSeq	NP_005954.3. NM_005963.3.
UniGene	Hs.689619.
3D structure databases
ProteinModelPortal	P12882.
ModBase	Search...
Protein-protein interaction databases
IntAct	P12882. 3 interactions.
STRING	9606.ENSP00000226207.
PTM databases
PhosphoSite	P12882.
Polymorphism databases
DMDM	226694176.
2D gel databases
UCD-2DPAGE	P12882.
Proteomic databases
PaxDb	P12882.
PRIDE	P12882.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000226207; ENSP00000226207; ENSG00000109061.
GeneID	4619.
KEGG	hsa:4619.
UCSC	uc002gmo.3. human.
Organism-specific databases
CTD	4619.
GeneCards	GC17M010395.
H-InvDB	HIX0027129.
HGNC	HGNC:7567. MYH1.
HPA	CAB010759. HPA001349.
MIM	160730. gene.
neXtProt	NX_P12882.
PharmGKB	PA31365.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG5022.
HOGENOM	HOG000173959.
HOVERGEN	HBG004704.
InParanoid	P12882.
KO	K10352.
OMA	QPTEEIS.
OrthoDB	EOG43N7BR.
PhylomeDB	P12882.
Gene expression databases
Bgee	P12882.
CleanEx	HS_MYH1.
Genevestigator	P12882.
GermOnline	ENSG00000109061. Homo sapiens.
Family and domain databases
InterPro	IPR000048. IQ_motif_EF-hand-BS. IPR015650. Myosin_1/23/4/7/8/13/15. IPR001609. Myosin_head_motor_dom. IPR004009. Myosin_N. IPR002928. Myosin_tail. [Graphical view]
PANTHER	PTHR13140:SF22. PTHR13140:SF22. 1 hit.
Pfam	PF00063. Myosin_head. 1 hit. PF02736. Myosin_N. 1 hit. PF01576. Myosin_tail_1. 1 hit. [Graphical view]
PRINTS	PR00193. MYOSINHEAVY.
SMART	SM00015. IQ. 1 hit. SM00242. MYSc. 1 hit. [Graphical view]
PROSITE	PS50096. IQ. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	4619.
NextBio	17780.
SOURCE	Search...

Entry information

Entry name

MYH1_HUMAN

Accession

Primary (citable) accession number: P12882
Secondary accession number(s): Q14CA4, Q9Y622

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	April 14, 2009
Last modified:	May 1, 2013
This is version 131 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.