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P12880 (CAPP_CORGL) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoenolpyruvate carboxylase

Short name=PEPC
Short name=PEPCase
EC=4.1.1.31
Gene names
Name:ppc
Ordered Locus Names:Cgl1585, cg1787
OrganismCorynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025) [Reference proteome] [HAMAP]
Taxonomic identifier196627 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium

Protein attributes

Sequence length919 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Forms oxaloacetate, a four-carbon dicarboxylic acid source for the tricarboxylic acid cycle. HAMAP-Rule MF_00595

Catalytic activity

Phosphate + oxaloacetate = H2O + phosphoenolpyruvate + HCO3-. HAMAP-Rule MF_00595

Cofactor

Magnesium By similarity. HAMAP-Rule MF_00595

Enzyme regulation

Activity not stimulated by acetyl-CoA in the absence of any allosteric inhibitor, while the corresponding protein from E.coli is strongly stimulated. HAMAP-Rule MF_00595

Sequence similarities

Belongs to the PEPCase type 1 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.2
Chain2 – 919918Phosphoenolpyruvate carboxylase HAMAP-Rule MF_00595
PRO_0000166591

Sites

Active site1381 By similarity
Active site5791 By similarity

Experimental info

Sequence conflict607 – 6082KL → NV in CAA32450. Ref.1
Sequence conflict800 – 8012FT → LP in CAA32450. Ref.1
Sequence conflict9151L → V in CAA32450. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P12880 [UniParc].

Last modified January 23, 2007. Version 4.
Checksum: 676C45D0EAAF3F54

FASTA919103,198
        10         20         30         40         50         60 
MTDFLRDDIR FLGQILGEVI AEQEGQEVYE LVEQARLTSF DIAKGNAEMD SLVQVFDGIT 

        70         80         90        100        110        120 
PAKATPIARA FSHFALLANL AEDLYDEELR EQALDAGDTP PDSTLDATWL KLNEGNVGAE 

       130        140        150        160        170        180 
AVADVLRNAE VAPVLTAHPT ETRRRTVFDA QKWITTHMRE RHALQSAEPT ARTQSKLDEI 

       190        200        210        220        230        240 
EKNIRRRITI LWQTALIRVA RPRIEDEIEV GLRYYKLSLL EEIPRINRDV AVELRERFGE 

       250        260        270        280        290        300 
GVPLKPVVKP GSWIGGDHDG NPYVTAETVE YSTHRAAETV LKYYARQLHS LEHELSLSDR 

       310        320        330        340        350        360 
MNKVTPQLLA LADAGHNDVP SRVDEPYRRA VHGVRGRILA TTAELIGEDA VEGVWFKVFT 

       370        380        390        400        410        420 
PYASPEEFLN DALTIDHSLR ESKDVLIADD RLSVLISAIE SFGFNLYALD LRQNSESYED 

       430        440        450        460        470        480 
VLTELFERAQ VTANYRELSE AEKLEVLLKE LRSPRPLIPH GSDEYSEVTD RELGIFRTAS 

       490        500        510        520        530        540 
EAVKKFGPRM VPHCIISMAS SVTDVLEPMV LLKEFGLIAA NGDNPRGTVD VIPLFETIED 

       550        560        570        580        590        600 
LQAGAGILDE LWKIDLYRNY LLQRDNVQEV MLGYSDSNKD GGYFSANWAL YDAELQLVEL 

       610        620        630        640        650        660 
CRSAGVKLRL FHGRGGTVGR GGGPSYDAIL AQPRGAVQGS VRITEQGEII SAKYGNPETA 

       670        680        690        700        710        720 
RRNLEALVSA TLEASLLDVS ELTDHQRAYD IMSEISELSL KKYASLVHED QGFIDYFTQS 

       730        740        750        760        770        780 
TPLQEIGSLN IGSRPSSRKQ TSSVEDLRAI PWVLSWSQSR VMLPGWFGVG TALEQWIGEG 

       790        800        810        820        830        840 
EQATQRIAEL QTLNESWPFF TSVLDNMAQV MSKAELRLAK LYADLIPDTE VAERVYSVIR 

       850        860        870        880        890        900 
EEYFLTKKMF CVITGSDDLL DDNPLLARSV QRRYPYLLPL NVIQVEMMRR YRKGDQSEQV 

       910 
SRNIQLTMNG LSTALRNSG 

« Hide

References

« Hide 'large scale' references
[1]"The phosphoenolpyruvate carboxylase gene of Corynebacterium glutamicum: molecular cloning, nucleotide sequence, and expression."
Eikmanns B.J., Follettie M.T., Griot M.U., Sinskey A.J.
Mol. Gen. Genet. 218:330-339(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 13059 / LMG 3658 / NCIB 10332 / AS019 / 613.
[2]"Cloning and nucleotide sequence of the phosphoenolpyruvate carboxylase-coding gene of Corynebacterium glutamicum ATCC13032."
O'Regan M., Thierbach G., Bachmann B., Villeval D., Lepage P., Viret J.F., Lemoine Y.
Gene 77:237-251(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-16.
Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
[3]"The Corynebacterium glutamicum genome: features and impacts on biotechnological processes."
Ikeda M., Nakagawa S.
Appl. Microbiol. Biotechnol. 62:99-109(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
[4]"The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins."
Kalinowski J., Bathe B., Bartels D., Bischoff N., Bott M., Burkovski A., Dusch N., Eggeling L., Eikmanns B.J., Gaigalat L., Goesmann A., Hartmann M., Huthmacher K., Kraemer R., Linke B., McHardy A.C., Meyer F., Moeckel B. expand/collapse author list , Pfefferle W., Puehler A., Rey D.A., Rueckert C., Rupp O., Sahm H., Wendisch V.F., Wiegraebe I., Tauch A.
J. Biotechnol. 104:5-25(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X14234 Genomic DNA. Translation: CAA32450.1.
M25819 Genomic DNA. Translation: AAA83537.1.
BA000036 Genomic DNA. Translation: BAB98978.1.
BX927152 Genomic DNA. Translation: CAF21593.1.
PIRQYFKG. S05512.
RefSeqNP_600799.1. NC_003450.3.
YP_225869.1. NC_006958.1.

3D structure databases

ProteinModelPortalP12880.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING196627.cg1787.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAB98978; BAB98978; BAB98978.
CAF21593; CAF21593; cg1787.
GeneID1019553.
KEGGcgb:cg1787.
cgl:NCgl1523.
PATRIC21495205. VBICorGlu203724_1546.

Phylogenomic databases

eggNOGCOG2352.
HOGENOMHOG000238647.
KOK01595.
OMAWYGAGSA.
OrthoDBEOG6TJ7T8.

Family and domain databases

HAMAPMF_00595. PEPcase_type1.
InterProIPR021135. PEP_COase.
IPR018129. PEP_COase_AS.
IPR022805. PEP_COase_bac/pln-type.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view]
PfamPF00311. PEPcase. 1 hit.
[Graphical view]
PRINTSPR00150. PEPCARBXLASE.
SUPFAMSSF51621. SSF51621. 1 hit.
PROSITEPS00781. PEPCASE_1. 1 hit.
PS00393. PEPCASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCAPP_CORGL
AccessionPrimary (citable) accession number: P12880
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: January 23, 2007
Last modified: July 9, 2014
This is version 107 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families