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P12880

- CAPP_CORGL

UniProt

P12880 - CAPP_CORGL

Protein

Phosphoenolpyruvate carboxylase

Gene

ppc

Organism
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 109 (01 Oct 2014)
      Sequence version 4 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Forms oxaloacetate, a four-carbon dicarboxylic acid source for the tricarboxylic acid cycle.

    Catalytic activityi

    Phosphate + oxaloacetate = H2O + phosphoenolpyruvate + HCO3-.

    Cofactori

    Magnesium.By similarity

    Enzyme regulationi

    Activity not stimulated by acetyl-CoA in the absence of any allosteric inhibitor, while the corresponding protein from E.coli is strongly stimulated.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei138 – 1381By similarity
    Active sitei579 – 5791By similarity

    GO - Molecular functioni

    1. magnesium ion binding Source: UniProtKB-HAMAP
    2. phosphoenolpyruvate carboxylase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. carbon fixation Source: UniProtKB-HAMAP
    2. oxaloacetate metabolic process Source: UniProtKB-HAMAP
    3. tricarboxylic acid cycle Source: InterPro

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Carbon dioxide fixation

    Keywords - Ligandi

    Magnesium

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphoenolpyruvate carboxylase (EC:4.1.1.31)
    Short name:
    PEPC
    Short name:
    PEPCase
    Gene namesi
    Name:ppc
    Ordered Locus Names:Cgl1585, cg1787
    OrganismiCorynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
    Taxonomic identifieri196627 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium
    ProteomesiUP000000582: Chromosome, UP000001009: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 919918Phosphoenolpyruvate carboxylasePRO_0000166591Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi196627.cg1787.

    Structurei

    3D structure databases

    ProteinModelPortaliP12880.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the PEPCase type 1 family.Curated

    Phylogenomic databases

    eggNOGiCOG2352.
    HOGENOMiHOG000238647.
    KOiK01595.
    OMAiWYGAGSA.
    OrthoDBiEOG6TJ7T8.

    Family and domain databases

    HAMAPiMF_00595. PEPcase_type1.
    InterProiIPR021135. PEP_COase.
    IPR018129. PEP_COase_AS.
    IPR022805. PEP_COase_bac/pln-type.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    [Graphical view]
    PfamiPF00311. PEPcase. 1 hit.
    [Graphical view]
    PRINTSiPR00150. PEPCARBXLASE.
    SUPFAMiSSF51621. SSF51621. 1 hit.
    PROSITEiPS00781. PEPCASE_1. 1 hit.
    PS00393. PEPCASE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P12880-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTDFLRDDIR FLGQILGEVI AEQEGQEVYE LVEQARLTSF DIAKGNAEMD    50
    SLVQVFDGIT PAKATPIARA FSHFALLANL AEDLYDEELR EQALDAGDTP 100
    PDSTLDATWL KLNEGNVGAE AVADVLRNAE VAPVLTAHPT ETRRRTVFDA 150
    QKWITTHMRE RHALQSAEPT ARTQSKLDEI EKNIRRRITI LWQTALIRVA 200
    RPRIEDEIEV GLRYYKLSLL EEIPRINRDV AVELRERFGE GVPLKPVVKP 250
    GSWIGGDHDG NPYVTAETVE YSTHRAAETV LKYYARQLHS LEHELSLSDR 300
    MNKVTPQLLA LADAGHNDVP SRVDEPYRRA VHGVRGRILA TTAELIGEDA 350
    VEGVWFKVFT PYASPEEFLN DALTIDHSLR ESKDVLIADD RLSVLISAIE 400
    SFGFNLYALD LRQNSESYED VLTELFERAQ VTANYRELSE AEKLEVLLKE 450
    LRSPRPLIPH GSDEYSEVTD RELGIFRTAS EAVKKFGPRM VPHCIISMAS 500
    SVTDVLEPMV LLKEFGLIAA NGDNPRGTVD VIPLFETIED LQAGAGILDE 550
    LWKIDLYRNY LLQRDNVQEV MLGYSDSNKD GGYFSANWAL YDAELQLVEL 600
    CRSAGVKLRL FHGRGGTVGR GGGPSYDAIL AQPRGAVQGS VRITEQGEII 650
    SAKYGNPETA RRNLEALVSA TLEASLLDVS ELTDHQRAYD IMSEISELSL 700
    KKYASLVHED QGFIDYFTQS TPLQEIGSLN IGSRPSSRKQ TSSVEDLRAI 750
    PWVLSWSQSR VMLPGWFGVG TALEQWIGEG EQATQRIAEL QTLNESWPFF 800
    TSVLDNMAQV MSKAELRLAK LYADLIPDTE VAERVYSVIR EEYFLTKKMF 850
    CVITGSDDLL DDNPLLARSV QRRYPYLLPL NVIQVEMMRR YRKGDQSEQV 900
    SRNIQLTMNG LSTALRNSG 919
    Length:919
    Mass (Da):103,198
    Last modified:January 23, 2007 - v4
    Checksum:i676C45D0EAAF3F54
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti607 – 6082KL → NV in CAA32450. (PubMed:2779518)Curated
    Sequence conflicti800 – 8012FT → LP in CAA32450. (PubMed:2779518)Curated
    Sequence conflicti915 – 9151L → V in CAA32450. (PubMed:2779518)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X14234 Genomic DNA. Translation: CAA32450.1.
    M25819 Genomic DNA. Translation: AAA83537.1.
    BA000036 Genomic DNA. Translation: BAB98978.1.
    BX927152 Genomic DNA. Translation: CAF21593.1.
    PIRiS05512. QYFKG.
    RefSeqiNP_600799.1. NC_003450.3.
    WP_011014465.1. NC_006958.1.
    YP_225869.1. NC_006958.1.

    Genome annotation databases

    EnsemblBacteriaiBAB98978; BAB98978; BAB98978.
    CAF21593; CAF21593; cg1787.
    GeneIDi1019553.
    KEGGicgb:cg1787.
    cgl:NCgl1523.
    PATRICi21495205. VBICorGlu203724_1546.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X14234 Genomic DNA. Translation: CAA32450.1 .
    M25819 Genomic DNA. Translation: AAA83537.1 .
    BA000036 Genomic DNA. Translation: BAB98978.1 .
    BX927152 Genomic DNA. Translation: CAF21593.1 .
    PIRi S05512. QYFKG.
    RefSeqi NP_600799.1. NC_003450.3.
    WP_011014465.1. NC_006958.1.
    YP_225869.1. NC_006958.1.

    3D structure databases

    ProteinModelPortali P12880.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 196627.cg1787.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAB98978 ; BAB98978 ; BAB98978 .
    CAF21593 ; CAF21593 ; cg1787 .
    GeneIDi 1019553.
    KEGGi cgb:cg1787.
    cgl:NCgl1523.
    PATRICi 21495205. VBICorGlu203724_1546.

    Phylogenomic databases

    eggNOGi COG2352.
    HOGENOMi HOG000238647.
    KOi K01595.
    OMAi WYGAGSA.
    OrthoDBi EOG6TJ7T8.

    Family and domain databases

    HAMAPi MF_00595. PEPcase_type1.
    InterProi IPR021135. PEP_COase.
    IPR018129. PEP_COase_AS.
    IPR022805. PEP_COase_bac/pln-type.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    [Graphical view ]
    Pfami PF00311. PEPcase. 1 hit.
    [Graphical view ]
    PRINTSi PR00150. PEPCARBXLASE.
    SUPFAMi SSF51621. SSF51621. 1 hit.
    PROSITEi PS00781. PEPCASE_1. 1 hit.
    PS00393. PEPCASE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The phosphoenolpyruvate carboxylase gene of Corynebacterium glutamicum: molecular cloning, nucleotide sequence, and expression."
      Eikmanns B.J., Follettie M.T., Griot M.U., Sinskey A.J.
      Mol. Gen. Genet. 218:330-339(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 13059 / LMG 3658 / NCIB 10332 / AS019 / 613.
    2. "Cloning and nucleotide sequence of the phosphoenolpyruvate carboxylase-coding gene of Corynebacterium glutamicum ATCC13032."
      O'Regan M., Thierbach G., Bachmann B., Villeval D., Lepage P., Viret J.F., Lemoine Y.
      Gene 77:237-251(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-16.
      Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
    3. "The Corynebacterium glutamicum genome: features and impacts on biotechnological processes."
      Ikeda M., Nakagawa S.
      Appl. Microbiol. Biotechnol. 62:99-109(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.

    Entry informationi

    Entry nameiCAPP_CORGL
    AccessioniPrimary (citable) accession number: P12880
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1989
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 109 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3