P12880 (CAPP_CORGL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoenolpyruvate carboxylase
Short name=PEPC
Short name=PEPCase
EC=4.1.1.31

Gene names

Name:	ppc
Ordered Locus Names:	Cgl1585, cg1787

Organism

Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025) [Reference proteome] [HAMAP]

Taxonomic identifier

196627 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium › Corynebacterium glutamicum

Protein attributes

Sequence length	919 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Forms oxaloacetate, a four-carbon dicarboxylic acid source for the tricarboxylic acid cycle. HAMAP-Rule MF_00595
Catalytic activity	Phosphate + oxaloacetate = H2O + phosphoenolpyruvate + HCO3-. HAMAP-Rule MF_00595
Cofactor	Magnesium By similarity.
Enzyme regulation	Activity not stimulated by acetyl-CoA in the absence of any allosteric inhibitor, while the corresponding protein from E.coli is strongly stimulated. HAMAP-Rule MF_00595
Sequence similarities	Belongs to the PEPCase type 1 family.

Ontologies

Keywords
Biological process	Carbon dioxide fixation
Ligand	Magnesium
Molecular function	Lyase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	carbon fixation Inferred from electronic annotation. Source: HAMAP oxaloacetate metabolic process Inferred from electronic annotation. Source: HAMAP tricarboxylic acid cycle Inferred from electronic annotation. Source: InterPro
Molecular_function	magnesium ion binding Inferred from electronic annotation. Source: HAMAP phosphoenolpyruvate carboxylase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.2
	Chain	2 – 919	918	Phosphoenolpyruvate carboxylase HAMAP-Rule MF_00595		PRO_0000166591
Sites
	Active site	138	1	By similarity
	Active site	579	1	By similarity
Experimental info
	Sequence conflict	607 – 608	2	KL → NV in CAA32450. Ref.1
	Sequence conflict	800 – 801	2	FT → LP in CAA32450. Ref.1
	Sequence conflict	915	1	L → V in CAA32450. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P12880 [UniParc]. Last modified January 23, 2007. Version 4. Checksum: 676C45D0EAAF3F54	FASTA	919	103,198
	10 20 30 40 50 60 MTDFLRDDIR FLGQILGEVI AEQEGQEVYE LVEQARLTSF DIAKGNAEMD SLVQVFDGIT 70 80 90 100 110 120 PAKATPIARA FSHFALLANL AEDLYDEELR EQALDAGDTP PDSTLDATWL KLNEGNVGAE 130 140 150 160 170 180 AVADVLRNAE VAPVLTAHPT ETRRRTVFDA QKWITTHMRE RHALQSAEPT ARTQSKLDEI 190 200 210 220 230 240 EKNIRRRITI LWQTALIRVA RPRIEDEIEV GLRYYKLSLL EEIPRINRDV AVELRERFGE 250 260 270 280 290 300 GVPLKPVVKP GSWIGGDHDG NPYVTAETVE YSTHRAAETV LKYYARQLHS LEHELSLSDR 310 320 330 340 350 360 MNKVTPQLLA LADAGHNDVP SRVDEPYRRA VHGVRGRILA TTAELIGEDA VEGVWFKVFT 370 380 390 400 410 420 PYASPEEFLN DALTIDHSLR ESKDVLIADD RLSVLISAIE SFGFNLYALD LRQNSESYED 430 440 450 460 470 480 VLTELFERAQ VTANYRELSE AEKLEVLLKE LRSPRPLIPH GSDEYSEVTD RELGIFRTAS 490 500 510 520 530 540 EAVKKFGPRM VPHCIISMAS SVTDVLEPMV LLKEFGLIAA NGDNPRGTVD VIPLFETIED 550 560 570 580 590 600 LQAGAGILDE LWKIDLYRNY LLQRDNVQEV MLGYSDSNKD GGYFSANWAL YDAELQLVEL 610 620 630 640 650 660 CRSAGVKLRL FHGRGGTVGR GGGPSYDAIL AQPRGAVQGS VRITEQGEII SAKYGNPETA 670 680 690 700 710 720 RRNLEALVSA TLEASLLDVS ELTDHQRAYD IMSEISELSL KKYASLVHED QGFIDYFTQS 730 740 750 760 770 780 TPLQEIGSLN IGSRPSSRKQ TSSVEDLRAI PWVLSWSQSR VMLPGWFGVG TALEQWIGEG 790 800 810 820 830 840 EQATQRIAEL QTLNESWPFF TSVLDNMAQV MSKAELRLAK LYADLIPDTE VAERVYSVIR 850 860 870 880 890 900 EEYFLTKKMF CVITGSDDLL DDNPLLARSV QRRYPYLLPL NVIQVEMMRR YRKGDQSEQV 910 SRNIQLTMNG LSTALRNSG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The phosphoenolpyruvate carboxylase gene of Corynebacterium glutamicum: molecular cloning, nucleotide sequence, and expression." Eikmanns B.J., Follettie M.T., Griot M.U., Sinskey A.J. Mol. Gen. Genet. 218:330-339(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 13059 / LMG 3658 / NCIB 10332 / AS019 / 613.
[2]	"Cloning and nucleotide sequence of the phosphoenolpyruvate carboxylase-coding gene of Corynebacterium glutamicum ATCC13032." O'Regan M., Thierbach G., Bachmann B., Villeval D., Lepage P., Viret J.F., Lemoine Y. Gene 77:237-251(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-16. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
[3]	"The Corynebacterium glutamicum genome: features and impacts on biotechnological processes." Ikeda M., Nakagawa S. Appl. Microbiol. Biotechnol. 62:99-109(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
[4]	"The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins." Kalinowski J., Bathe B., Bartels D., Bischoff N., Bott M., Burkovski A., Dusch N., Eggeling L., Eikmanns B.J., Gaigalat L., Goesmann A., Hartmann M., Huthmacher K., Kraemer R., Linke B., McHardy A.C., Meyer F., Moeckel B. , Pfefferle W., Puehler A., Rey D.A., Rueckert C., Rupp O., Sahm H., Wendisch V.F., Wiegraebe I., Tauch A. J. Biotechnol. 104:5-25(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X14234 Genomic DNA. Translation: CAA32450.1. M25819 Genomic DNA. Translation: AAA83537.1. BA000036 Genomic DNA. Translation: BAB98978.1. BX927152 Genomic DNA. Translation: CAF21593.1.
PIR	QYFKG. S05512.
RefSeq	NP_600799.1. NC_003450.3. YP_225869.1. NC_006958.1.
3D structure databases
ProteinModelPortal	P12880.
ModBase	Search...
Protein-protein interaction databases
STRING	196627.cg1787.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAB98978; BAB98978; BAB98978. CAF21593; CAF21593; cg1787.
GeneID	1019553. 3343481.
KEGG	cgb:cg1787. cgl:NCgl1523.
PATRIC	21495205. VBICorGlu203724_1546.
Phylogenomic databases
eggNOG	COG2352.
HOGENOM	HOG000238647.
KO	K01595.
OMA	AIPWVFG.
ProtClustDB	PRK00009.
Enzyme and pathway databases
BioCyc	CGLU196627:GJDM-1568-MONOMER.
Family and domain databases
HAMAP	MF_00595. PEPcase_type1.
InterPro	IPR021135. PEP_COase. IPR018129. PEP_COase_AS. IPR022805. PEP_COase_bac/pln-type. IPR015813. Pyrv/PenolPyrv_Kinase. [Graphical view]
Pfam	PF00311. PEPcase. 1 hit. [Graphical view]
PRINTS	PR00150. PEPCARBXLASE.
SUPFAM	SSF51621. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
PROSITE	PS00781. PEPCASE_1. 1 hit. PS00393. PEPCASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CAPP_CORGL

Accession

Primary (citable) accession number: P12880

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 101 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program