P12877 (RL5_BACSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
50S ribosomal protein L5

Alternative name(s):
BL6

Gene names

Name:	rplE
Ordered Locus Names:	BSU01280

Organism

Bacillus subtilis (strain 168) [Reference proteome] [HAMAP]

Taxonomic identifier

224308 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › Bacillus subtilis group › Bacillus subtilis › Bacillus subtilis subsp. subtilis

Protein attributes

Sequence length	179 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This is 1 of the proteins that binds and probably mediates the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance. In the 70S ribosome it contacts protein S13 of the 30S subunit (bridge B1b), connecting the 2 subunits; this bridge is implicated in subunit movement. Contacts the P site tRNA; the 5S rRNA and some of its associated proteins might help stabilize positioning of ribosome-bound tRNAs By similarity. HAMAP-Rule MF_01333
Subunit structure	Part of the 50S ribosomal subunit; part of the 5S rRNA/L5/L18/L25 subcomplex. Contacts the 5S rRNA and the P site tRNA. Forms a bridge to the 30S subunit in the 70S ribosome By similarity.
Sequence similarities	Belongs to the ribosomal protein L5P family.

Ontologies

Keywords
Ligand	RNA-binding rRNA-binding tRNA-binding
Molecular function	Ribonucleoprotein Ribosomal protein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	translation Inferred from electronic annotation. Source: HAMAP
Cellular_component	ribosome Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	rRNA binding Inferred from electronic annotation. Source: HAMAP structural constituent of ribosome Inferred from electronic annotation. Source: InterPro tRNA binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 179	179	50S ribosomal protein L5 HAMAP-Rule MF_01333		PRO_0000124892

Sequences

	Sequence		Length	Mass (Da)
	P12877 [UniParc]. Last modified October 1, 1989. Version 1. Checksum: 57576598B0A7979B	FASTA	179	20,148
	10 20 30 40 50 60 MNRLKEKYNK EIAPALMTKF NYDSVMQVPK IEKIVINMGV GDAVQNAKAI DSAVEELTFI 70 80 90 100 110 120 AGQKPVVTRA KKSIAGFRLR EGMPIGAKVT LRGERMYDFL DKLISVSLPR VRDFRGVSKK 130 140 150 160 170 SFDGRGNYTL GIKEQLIFPE IDYDKVTKVR GMDIVIVTTA NTDEEARELL TQVGMPFQK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and analysis of the spc ribosomal protein operon of Bacillus subtilis: comparison with the spc operon of Escherichia coli." Henkin T.M., Moon S.H., Mattheakis L.C., Nomura M. Nucleic Acids Res. 17:7469-7486(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168.
[2]	"Genetic and transcriptional organization of the Bacillus subtilis spc-alpha region." Suh J.-W., Boylan S.A., Oh S.H., Price C.W. Gene 169:17-23(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB 3610 / VKM B-501.
[3]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X15664 Genomic DNA. Translation: CAA33703.1. L47971 Genomic DNA. Translation: AAB06811.1. AL009126 Genomic DNA. Translation: CAB11904.1.
PIR	R5BS5. S05994.
RefSeq	NP_388009.1. NC_000964.3.
3D structure databases
ProteinModelPortal	P12877.
SMR	P12877. Positions 1-179.
ModBase	Search...
Protein-protein interaction databases
STRING	224308.BSU01280.
Proteomic databases
PaxDb	P12877.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAB11904; CAB11904; BSU01280.
GeneID	936981.
KEGG	bsu:BSU01280.
PATRIC	18971765. VBIBacSub10457_0131.
Organism-specific databases
GenoList	BSU01280. [Micado]
Phylogenomic databases
eggNOG	COG0094.
HOGENOM	HOG000231311.
KO	K02931.
OMA	EQVMFHE.
ProtClustDB	PRK00010.
Enzyme and pathway databases
BioCyc	BSUB:BSU01280-MONOMER.
Family and domain databases
Gene3D	3.30.1440.10. 1 hit.
HAMAP	MF_01333_B. Ribosomal_L5_B.
InterPro	IPR002132. Ribosomal_L5. IPR020930. Ribosomal_L5_bac-type. IPR020929. Ribosomal_L5_CS. IPR022803. Ribosomal_L5_domain. [Graphical view]
PANTHER	PTHR11994. PTHR11994. 1 hit.
Pfam	PF00281. Ribosomal_L5. 1 hit. PF00673. Ribosomal_L5_C. 1 hit. [Graphical view]
PIRSF	PIRSF002161. Ribosomal_L5. 1 hit.
SUPFAM	SSF55282. Ribosomal_L5. 1 hit.
PROSITE	PS00358. RIBOSOMAL_L5. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RL5_BACSU

Accession

Primary (citable) accession number: P12877

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	October 1, 1989
Last modified:	May 1, 2013
This is version 100 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program