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P12869

- CAPSD_BOOLV

UniProt

P12869 - CAPSD_BOOLV

Protein

Capsid protein alpha

Gene

alpha

Organism
Boolarra virus (BoV)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 81 (01 Oct 2014)
      Sequence version 1 (01 Oct 1989)
      Previous versions | rss
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    Functioni

    Capsid protein alpha self-assembles to form an icosahedral procapsid with a T=3 symmetry, about 30 nm in diameter, and consisting of 60 capsid proteins trimers. The capsid encapsulates the two genomic RNAs. Capsid maturation occurs via autoproteolytic cleavage of capsid protein alpha generating capsid protein beta and the membrane-active peptide gamma By similarity.By similarity
    Peptide gamma: membrane-permeabilizing peptide produced by virus maturation, thereby creating the infectious virion. After endocytosis into the host cell, peptide gamma is probably exposed in endosomes, where it permeabilizes the endosomal membrane, facilitating translocation of viral capsid or RNA into the cytoplasm By similarity.By similarity

    Catalytic activityi

    Hydrolysis of an asparaginyl bond involved in the maturation of the structural protein of the virus, typically -Asn-|-Ala- or -Asn-|-Phe-.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei61 – 611By similarity

    GO - Molecular functioni

    1. aspartic-type endopeptidase activity Source: UniProtKB-KW

    GO - Biological processi

    1. permeabilization of host organelle membrane involved in viral entry into host cell Source: UniProtKB-KW

    Keywords - Molecular functioni

    Aspartyl protease, Hydrolase, Protease

    Keywords - Biological processi

    Viral penetration into host cytoplasm, Viral penetration via permeabilization of host membrane, Virus entry into host cell

    Protein family/group databases

    MEROPSiN01.001.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Capsid protein alpha
    Cleaved into the following 2 chains:
    Alternative name(s):
    Coat protein beta
    Nodavirus endopeptidase
    Alternative name(s):
    Coat protein gamma
    Gene namesi
    Name:alpha
    OrganismiBoolarra virus (BoV)
    Taxonomic identifieri12286 [NCBI]
    Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stageNodaviridaeAlphanodavirus
    Virus hostiHepialidae (ghost moths) [TaxID: 41021]

    Subcellular locationi

    Chain Peptide gamma : Virion Curated
    Note: Located inside the capsid and probably externalized in early endosomes.Curated

    GO - Cellular componenti

    1. T=3 icosahedral viral capsid Source: UniProtKB-KW

    Keywords - Cellular componenti

    Capsid protein, T=3 icosahedral capsid protein, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 403403Capsid protein alphaPRO_0000402387Add
    BLAST
    Chaini1 – 359359Capsid protein betaPRO_0000039192Add
    BLAST
    Chaini360 – 40344Peptide gammaPRO_0000039193Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi55 ↔ 314By similarity

    Post-translational modificationi

    Capsid protein alpha autocatalytically maturates into capsid protein beta and peptide gamma.By similarity

    Keywords - PTMi

    Disulfide bond

    Structurei

    3D structure databases

    ProteinModelPortaliP12869.
    SMRiP12869. Positions 42-359.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi25 – 3410Poly-Arg

    Sequence similaritiesi

    Belongs to the peptidase A6 family.Curated

    Family and domain databases

    Gene3Di2.60.120.20. 1 hit.
    InterProiIPR000696. Peptidase_A6.
    IPR029053. Viral_coat.
    [Graphical view]
    PfamiPF01829. Peptidase_A6. 1 hit.
    [Graphical view]
    PRINTSiPR00863. NODAVIRPTASE.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P12869-1 [UniParc]FASTAAdd to Basket

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    MTPRRQQRPK GQLAKAKQAK QPLARSRRPR RRRRAAITQN NLMMLSEPGL    50
    SFLKCAFASP DSNTDPGKGI PDNFEGKVLS QKNVYTETGV NFSGATTQNV 100
    DTYIIVLPTP GVAFWRCIKT ATAPAQPAAL TTTDVFTAVP FPDFTSLFGT 150
    TATNRADQVA AFRYASMNFG LYPTCNSTQY NGGISVWKGA VQMSTTQYPL 200
    DTTPESSQLV HAITGLESAL KVGDENYSES FIDGVFTQSI NGNAEFPFYP 250
    ILEGVQTLPG QNVTVAQAGM PFSLDAGAAT VAGFTGIGGM DAIFIKVTAA 300
    AGSVNTATIK TWACIEYRPN TNTALYKYAH DSPAEDIIAL QQYRKVYKSL 350
    PVAVRAKLNA NMWERVKRLL KAGLVAASYV PGPVGGIATG VQHIGDLIAE 400
    LSF 403
    Length:403
    Mass (Da):43,357
    Last modified:October 1, 1989 - v1
    Checksum:i73C3533D238B1EE9
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X15960 Genomic RNA. Translation: CAA34082.1.
    PIRiA34011. VCBBBL.
    RefSeqiNP_689443.1. NC_004145.1.

    Genome annotation databases

    GeneIDi956658.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X15960 Genomic RNA. Translation: CAA34082.1 .
    PIRi A34011. VCBBBL.
    RefSeqi NP_689443.1. NC_004145.1.

    3D structure databases

    ProteinModelPortali P12869.
    SMRi P12869. Positions 42-359.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi N01.001.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 956658.

    Family and domain databases

    Gene3Di 2.60.120.20. 1 hit.
    InterProi IPR000696. Peptidase_A6.
    IPR029053. Viral_coat.
    [Graphical view ]
    Pfami PF01829. Peptidase_A6. 1 hit.
    [Graphical view ]
    PRINTSi PR00863. NODAVIRPTASE.
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequences of three Nodavirus RNA2's: the messengers for their coat protein precursors."
      Dasgupta R., Sgro J.-Y.
      Nucleic Acids Res. 17:7525-7526(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    2. "Structural homology among four nodaviruses as deduced by sequencing and X-ray crystallography."
      Kaesberg P., Dasgupta R., Sgro J.-Y., Wery J.-P., Selling B.H., Hosur M.V., Johnson J.E.
      J. Mol. Biol. 214:423-435(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: SIMILARITY TO OTHER NODAVIRUSES.

    Entry informationi

    Entry nameiCAPSD_BOOLV
    AccessioniPrimary (citable) accession number: P12869
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1989
    Last sequence update: October 1, 1989
    Last modified: October 1, 2014
    This is version 81 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3