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P12869 (CAPSD_BOOLV) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Capsid protein alpha

Cleaved into the following 2 chains:

  1. Capsid protein beta
    EC=3.4.23.44
    Alternative name(s):
    Coat protein beta
    Nodavirus endopeptidase
  2. Peptide gamma
    Alternative name(s):
    Coat protein gamma
Gene names
Name:alpha
OrganismBoolarra virus (BoV)
Taxonomic identifier12286 [NCBI]
Taxonomic lineageVirusesssRNA positive-strand viruses, no DNA stageNodaviridaeAlphanodavirus
Virus hostHepialidae (ghost moths) [TaxID: 41021]

Protein attributes

Sequence length403 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Capsid protein alpha self-assembles to form an icosahedral procapsid with a T=3 symmetry, about 30 nm in diameter, and consisting of 60 capsid proteins trimers. The capsid encapsulates the two genomic RNAs. Capsid maturation occurs via autoproteolytic cleavage of capsid protein alpha generating capsid protein beta and the membrane-active peptide gamma By similarity.

Peptide gamma: membrane-permeabilizing peptide produced by virus maturation, thereby creating the infectious virion. After endocytosis into the host cell, peptide gamma is probably exposed in endosomes, where it permeabilizes the endosomal membrane, facilitating translocation of viral capsid or RNA into the cytoplasm By similarity.

Catalytic activity

Hydrolysis of an asparaginyl bond involved in the maturation of the structural protein of the virus, typically -Asn-|-Ala- or -Asn-|-Phe-.

Subcellular location

Capsid protein beta: Virion Potential.

Peptide gamma: Virion Potential. Note: Located inside the capsid and probably externalized in early endosomes Potential.

Post-translational modification

Capsid protein alpha autocatalytically maturates into capsid protein beta and peptide gamma By similarity.

Sequence similarities

Belongs to the peptidase A6 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 403403Capsid protein alpha
PRO_0000402387
Chain1 – 359359Capsid protein beta
PRO_0000039192
Chain360 – 40344Peptide gamma
PRO_0000039193

Regions

Compositional bias25 – 3410Poly-Arg

Sites

Active site611 By similarity

Amino acid modifications

Disulfide bond55 ↔ 314 By similarity

Sequences

Sequence LengthMass (Da)Tools
P12869 [UniParc].

Last modified October 1, 1989. Version 1.
Checksum: 73C3533D238B1EE9

FASTA40343,357
        10         20         30         40         50         60 
MTPRRQQRPK GQLAKAKQAK QPLARSRRPR RRRRAAITQN NLMMLSEPGL SFLKCAFASP 

        70         80         90        100        110        120 
DSNTDPGKGI PDNFEGKVLS QKNVYTETGV NFSGATTQNV DTYIIVLPTP GVAFWRCIKT 

       130        140        150        160        170        180 
ATAPAQPAAL TTTDVFTAVP FPDFTSLFGT TATNRADQVA AFRYASMNFG LYPTCNSTQY 

       190        200        210        220        230        240 
NGGISVWKGA VQMSTTQYPL DTTPESSQLV HAITGLESAL KVGDENYSES FIDGVFTQSI 

       250        260        270        280        290        300 
NGNAEFPFYP ILEGVQTLPG QNVTVAQAGM PFSLDAGAAT VAGFTGIGGM DAIFIKVTAA 

       310        320        330        340        350        360 
AGSVNTATIK TWACIEYRPN TNTALYKYAH DSPAEDIIAL QQYRKVYKSL PVAVRAKLNA 

       370        380        390        400 
NMWERVKRLL KAGLVAASYV PGPVGGIATG VQHIGDLIAE LSF 

« Hide

References

[1]"Nucleotide sequences of three Nodavirus RNA2's: the messengers for their coat protein precursors."
Dasgupta R., Sgro J.-Y.
Nucleic Acids Res. 17:7525-7526(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]"Structural homology among four nodaviruses as deduced by sequencing and X-ray crystallography."
Kaesberg P., Dasgupta R., Sgro J.-Y., Wery J.-P., Selling B.H., Hosur M.V., Johnson J.E.
J. Mol. Biol. 214:423-435(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: SIMILARITY TO OTHER NODAVIRUSES.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X15960 Genomic RNA. Translation: CAA34082.1.
PIRVCBBBL. A34011.
RefSeqNP_689443.1. NC_004145.1.

3D structure databases

ProteinModelPortalP12869.
SMRP12869. Positions 42-359.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSN01.001.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID956658.

Family and domain databases

InterProIPR000696. Peptidase_A6.
[Graphical view]
PfamPF01829. Peptidase_A6. 1 hit.
[Graphical view]
PRINTSPR00863. NODAVIRPTASE.
ProtoNetSearch...

Entry information

Entry nameCAPSD_BOOLV
AccessionPrimary (citable) accession number: P12869
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 1, 1989
Last modified: February 19, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries