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P12868

- PEP5_YEAST

UniProt

P12868 - PEP5_YEAST

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Protein
Vacuolar membrane protein PEP5
Gene
PEP5, END1, VAM1, VPL9, VPS11, VPT11, YMR231W, YM9959.13
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Required for vacuolar biogenesis and for trafficking of hydrolase precursors to the vacuole. Mediates transport at the vacuolar membrane where it may be responsible for tethering transport vesicles on the target membranes. It is required for gluconeogenic growth of yeast. Acts as component of the HOPS complex that acts during the docking stage of vacuole fusion. HOPS is an effector for the vacuolar Rab GTPase YPT7 and is required for vacuolar SNARE complex assembly. It remains bound to SNARE complexes after vacuole fusion.2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri928 – 97346RING-type
Add
BLAST

GO - Molecular functioni

  1. protein binding Source: IntAct
  2. ubiquitin-protein transferase activity Source: SGD
  3. zinc ion binding Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. Golgi to endosome transport Source: SGD
  2. histone catabolic process Source: SGD
  3. histone ubiquitination Source: SGD
  4. intracellular protein transport Source: InterPro
  5. late endosome to vacuole transport Source: SGD
  6. regulation of SNARE complex assembly Source: SGD
  7. regulation of vacuole fusion, non-autophagic Source: SGD
  8. vacuole fusion, non-autophagic Source: SGD
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-32912-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar membrane protein PEP5
Alternative name(s):
Carboxypeptidase Y-deficient protein 5
Vacuolar biogenesis protein END1
Vacuolar morphogenesis protein 1
Vacuolar protein sorting-associated protein 11
Vacuolar protein-targeting protein 11
Gene namesi
Name:PEP5
Synonyms:END1, VAM1, VPL9, VPS11, VPT11
Ordered Locus Names:YMR231W
ORF Names:YM9959.13
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIII

Organism-specific databases

CYGDiYMR231w.
SGDiS000004844. PEP5.

Subcellular locationi

GO - Cellular componenti

  1. CORVET complex Source: SGD
  2. HOPS complex Source: SGD
  3. fungal-type vacuole membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10291029Vacuolar membrane protein PEP5
PRO_0000055995Add
BLAST

Proteomic databases

MaxQBiP12868.
PaxDbiP12868.

Expressioni

Gene expression databases

GenevestigatoriP12868.

Interactioni

Subunit structurei

Component of the HOPS complex which is composed of PEP5, VPS16, PEP3, VPS33, VPS39 and VPS41. HOPS associates with phosphoinositides and the PX domain of VAM7. Interacts with PEP3, PEP7 and VAM7.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
PEP3P278017EBI-6450,EBI-13130
VPS33P207955EBI-6450,EBI-20395

Protein-protein interaction databases

BioGridi35409. 63 interactions.
DIPiDIP-4409N.
IntActiP12868. 13 interactions.
MINTiMINT-545862.
STRINGi4932.YMR231W.

Structurei

3D structure databases

ProteinModelPortaliP12868.
SMRiP12868. Positions 927-977.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati406 – 583178CHCR
Add
BLAST

Sequence similaritiesi

Belongs to the VPS11 family.

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiNOG294563.
GeneTreeiENSGT00390000015058.
HOGENOMiHOG000074719.
OMAiGMHEDVL.
OrthoDBiEOG7GN2W1.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
3.30.40.10. 1 hit.
InterProiIPR000547. Clathrin_H-chain/VPS_repeat.
IPR016528. VPS11.
IPR024763. VPS11_C.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR017986. WD40_repeat_dom.
IPR001965. Znf_PHD.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view]
PfamiPF12451. VPS11_C. 1 hit.
[Graphical view]
PIRSFiPIRSF007860. VPS11. 1 hit.
SMARTiSM00249. PHD. 1 hit.
SM00184. RING. 1 hit.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS50236. CHCR. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P12868-1 [UniParc]FASTAAdd to Basket

« Hide

MSLSSWRQFQ LFENIPIRDP NFGGDSLLYS DPTLCAATIV DPQTLIIAVN     50
SNIIKVVKLN QSQVIHEFQS FPHDFQITFL KVINGEFLVA LAESIGKPSL 100
IRVYKLEKLP NREQLYHSQV ELKNGNNTYP ISVVSISNDL SCIVVGFING 150
KIILIRGDIS RDRGSQQRII YEDPSKEPIT ALFLNNDATA CFAATTSRIL 200
LFNTTGRNRG RPSLVLNSKN GLDLNCGSFN PATNEFICCL SNFIEFFSSS 250
GKKHQFAFDL SLRKRIFCVD KDHILIVTEE TGVPTTSISV NELSPTIINR 300
IFIIDAKNKI ISLNFVVSSA IIDIFSTSQS GKNITYLLTS EGVMHRITPK 350
SLENQINIII QKELYPFALQ LAKQHSLSPL DVQEIHKKYG DYLFKKGLRK 400
EATDQYIQCL DVVETSEIIS KFGVKEVPDP ESMRNLADYL WSLIKNSISQ 450
RDHVTLLLIV LIKLKDVEGI DTFIQHFDRK GIWNEGVVMD DMDDVTFFYS 500
DNDFFDLDLI LELMKESDFK RLSYRLAKKY SKDSLIIVDI LLNLLHNPVK 550
AIKYIKSLPI DETLRCLVTY SKKLLEESPN ETNALLIEVF TGKFKPSTFE 600
VDLDRRDTTG DFSENIRTVF YSYKTFFNYM NSNGTSDAMS ESSEASHEHE 650
EPTYHPPKPS IVFSSFVTKP FEFVVFLEAC LACYQQYEGF DEDRQVILTT 700
LYDLYLNLAQ NDVPERIDDW RSRATGVLRE SNKLVYSAAS NNTSKRVDNS 750
IMLLISHMDQ SSASAKDKTK IDIASFANDN PEMDLLSTFR AMTLNEEPST 800
CLKFLEKYGT EEPKLLQVAL SYFVSNKLIF KEMGGNEVLK EKVLRPIIEG 850
ERMPLLDIIK ALSRTNVAHF GLIQDIIIDH VKTEDTEIKR NEKLIESYDK 900
ELKEKNKKLK NTINSDQPLH VPLKNQTCFM CRLTLDIPVV FFKCGHIYHQ 950
HCLNEEEDTL ESERKLFKCP KCLVDLETSN KLFEAQHEVV EKNDLLNFAL 1000
NSEEGSRDRF KVITEFLGRG AISYSDITI 1029
Length:1,029
Mass (Da):117,478
Last modified:August 1, 1991 - v2
Checksum:iE5216AD3C285CF34
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti37 – 371A → R in CAA33413. 1 Publication
Sequence conflicti619 – 6257VFYSYKT → MFFTVTKH in CAA33413. 1 Publication
Sequence conflicti769 – 7702TK → KQ in CAA33413. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X54466 Genomic DNA. Translation: CAA38348.1.
X15355 Genomic DNA. Translation: CAA33413.1.
Z49939 Genomic DNA. Translation: CAA90202.1.
BK006946 Genomic DNA. Translation: DAA10131.1.
PIRiS57598.
RefSeqiNP_013958.1. NM_001182738.1.

Genome annotation databases

EnsemblFungiiYMR231W; YMR231W; YMR231W.
GeneIDi855271.
KEGGisce:YMR231W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X54466 Genomic DNA. Translation: CAA38348.1 .
X15355 Genomic DNA. Translation: CAA33413.1 .
Z49939 Genomic DNA. Translation: CAA90202.1 .
BK006946 Genomic DNA. Translation: DAA10131.1 .
PIRi S57598.
RefSeqi NP_013958.1. NM_001182738.1.

3D structure databases

ProteinModelPortali P12868.
SMRi P12868. Positions 927-977.
ModBasei Search...

Protein-protein interaction databases

BioGridi 35409. 63 interactions.
DIPi DIP-4409N.
IntActi P12868. 13 interactions.
MINTi MINT-545862.
STRINGi 4932.YMR231W.

Proteomic databases

MaxQBi P12868.
PaxDbi P12868.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YMR231W ; YMR231W ; YMR231W .
GeneIDi 855271.
KEGGi sce:YMR231W.

Organism-specific databases

CYGDi YMR231w.
SGDi S000004844. PEP5.

Phylogenomic databases

eggNOGi NOG294563.
GeneTreei ENSGT00390000015058.
HOGENOMi HOG000074719.
OMAi GMHEDVL.
OrthoDBi EOG7GN2W1.

Enzyme and pathway databases

BioCyci YEAST:G3O-32912-MONOMER.

Miscellaneous databases

NextBioi 978885.
PROi P12868.

Gene expression databases

Genevestigatori P12868.

Family and domain databases

Gene3Di 2.130.10.10. 1 hit.
3.30.40.10. 1 hit.
InterProi IPR000547. Clathrin_H-chain/VPS_repeat.
IPR016528. VPS11.
IPR024763. VPS11_C.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR017986. WD40_repeat_dom.
IPR001965. Znf_PHD.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
[Graphical view ]
Pfami PF12451. VPS11_C. 1 hit.
[Graphical view ]
PIRSFi PIRSF007860. VPS11. 1 hit.
SMARTi SM00249. PHD. 1 hit.
SM00184. RING. 1 hit.
[Graphical view ]
SUPFAMi SSF50978. SSF50978. 1 hit.
PROSITEi PS50236. CHCR. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation and characterization of PEP5, a gene essential for vacuolar biogenesis in Saccharomyces cerevisiae."
    Woolford C.A., Dixon C.K., Manolson M.F., Wright R., Jones E.W.
    Genetics 125:739-752(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Characterization of the END1 gene required for vacuole biogenesis and gluconeogenic growth of budding yeast."
    Dulic V., Riezman H.
    EMBO J. 8:1349-1359(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "Pep3p/Pep5p complex: a putative docking factor at multiple steps of vesicular transport to the vacuole of Saccharomyces cerevisiae."
    Srivastava A., Woolford C.A., Jones E.W.
    Genetics 156:105-122(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH PEP3 AND PEP7.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "Purification of active HOPS complex reveals its affinities for phosphoinositides and the SNARE Vam7p."
    Stroupe C., Collins K.M., Fratti R.A., Wickner W.
    EMBO J. 25:1579-1589(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE HOPS COMPLEX, FUNCTION OF THE HOPS COMPLEX, INTERACTION WITH VAM7.
  8. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPEP5_YEAST
AccessioniPrimary (citable) accession number: P12868
Secondary accession number(s): D6W057
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: August 1, 1991
Last modified: September 3, 2014
This is version 137 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1200 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3

Similar proteinsi