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P12868

- PEP5_YEAST

UniProt

P12868 - PEP5_YEAST

Protein

Vacuolar membrane protein PEP5

Gene

PEP5

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 138 (01 Oct 2014)
      Sequence version 2 (01 Aug 1991)
      Previous versions | rss
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    Functioni

    Required for vacuolar biogenesis and for trafficking of hydrolase precursors to the vacuole. Mediates transport at the vacuolar membrane where it may be responsible for tethering transport vesicles on the target membranes. It is required for gluconeogenic growth of yeast. Acts as component of the HOPS complex that acts during the docking stage of vacuole fusion. HOPS is an effector for the vacuolar Rab GTPase YPT7 and is required for vacuolar SNARE complex assembly. It remains bound to SNARE complexes after vacuole fusion.2 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri928 – 97346RING-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. ubiquitin-protein transferase activity Source: SGD
    3. zinc ion binding Source: InterPro

    GO - Biological processi

    1. Golgi to endosome transport Source: SGD
    2. histone catabolic process Source: SGD
    3. histone ubiquitination Source: SGD
    4. intracellular protein transport Source: InterPro
    5. late endosome to vacuole transport Source: SGD
    6. regulation of SNARE complex assembly Source: SGD
    7. regulation of vacuole fusion, non-autophagic Source: SGD
    8. vacuole fusion, non-autophagic Source: SGD

    Keywords - Biological processi

    Protein transport, Transport

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32912-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Vacuolar membrane protein PEP5
    Alternative name(s):
    Carboxypeptidase Y-deficient protein 5
    Vacuolar biogenesis protein END1
    Vacuolar morphogenesis protein 1
    Vacuolar protein sorting-associated protein 11
    Vacuolar protein-targeting protein 11
    Gene namesi
    Name:PEP5
    Synonyms:END1, VAM1, VPL9, VPS11, VPT11
    Ordered Locus Names:YMR231W
    ORF Names:YM9959.13
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIII

    Organism-specific databases

    CYGDiYMR231w.
    SGDiS000004844. PEP5.

    Subcellular locationi

    GO - Cellular componenti

    1. CORVET complex Source: SGD
    2. fungal-type vacuole membrane Source: SGD
    3. HOPS complex Source: SGD

    Keywords - Cellular componenti

    Membrane, Vacuole

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10291029Vacuolar membrane protein PEP5PRO_0000055995Add
    BLAST

    Proteomic databases

    MaxQBiP12868.
    PaxDbiP12868.

    Expressioni

    Gene expression databases

    GenevestigatoriP12868.

    Interactioni

    Subunit structurei

    Component of the HOPS complex which is composed of PEP5, VPS16, PEP3, VPS33, VPS39 and VPS41. HOPS associates with phosphoinositides and the PX domain of VAM7. Interacts with PEP3, PEP7 and VAM7.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    PEP3P278017EBI-6450,EBI-13130
    VPS33P207955EBI-6450,EBI-20395

    Protein-protein interaction databases

    BioGridi35409. 63 interactions.
    DIPiDIP-4409N.
    IntActiP12868. 13 interactions.
    MINTiMINT-545862.
    STRINGi4932.YMR231W.

    Structurei

    3D structure databases

    ProteinModelPortaliP12868.
    SMRiP12868. Positions 927-977.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati406 – 583178CHCRAdd
    BLAST

    Sequence similaritiesi

    Belongs to the VPS11 family.Curated
    Contains 1 CHCR (clathrin heavy-chain) repeat.PROSITE-ProRule annotation
    Contains 1 RING-type zinc finger.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri928 – 97346RING-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiNOG294563.
    GeneTreeiENSGT00390000015058.
    HOGENOMiHOG000074719.
    OMAiGMHEDVL.
    OrthoDBiEOG7GN2W1.

    Family and domain databases

    Gene3Di2.130.10.10. 1 hit.
    3.30.40.10. 1 hit.
    InterProiIPR000547. Clathrin_H-chain/VPS_repeat.
    IPR016528. VPS11.
    IPR024763. VPS11_C.
    IPR015943. WD40/YVTN_repeat-like_dom.
    IPR017986. WD40_repeat_dom.
    IPR001965. Znf_PHD.
    IPR001841. Znf_RING.
    IPR013083. Znf_RING/FYVE/PHD.
    [Graphical view]
    PfamiPF12451. VPS11_C. 1 hit.
    [Graphical view]
    PIRSFiPIRSF007860. VPS11. 1 hit.
    SMARTiSM00249. PHD. 1 hit.
    SM00184. RING. 1 hit.
    [Graphical view]
    SUPFAMiSSF50978. SSF50978. 1 hit.
    PROSITEiPS50236. CHCR. 1 hit.
    PS50089. ZF_RING_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P12868-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSLSSWRQFQ LFENIPIRDP NFGGDSLLYS DPTLCAATIV DPQTLIIAVN     50
    SNIIKVVKLN QSQVIHEFQS FPHDFQITFL KVINGEFLVA LAESIGKPSL 100
    IRVYKLEKLP NREQLYHSQV ELKNGNNTYP ISVVSISNDL SCIVVGFING 150
    KIILIRGDIS RDRGSQQRII YEDPSKEPIT ALFLNNDATA CFAATTSRIL 200
    LFNTTGRNRG RPSLVLNSKN GLDLNCGSFN PATNEFICCL SNFIEFFSSS 250
    GKKHQFAFDL SLRKRIFCVD KDHILIVTEE TGVPTTSISV NELSPTIINR 300
    IFIIDAKNKI ISLNFVVSSA IIDIFSTSQS GKNITYLLTS EGVMHRITPK 350
    SLENQINIII QKELYPFALQ LAKQHSLSPL DVQEIHKKYG DYLFKKGLRK 400
    EATDQYIQCL DVVETSEIIS KFGVKEVPDP ESMRNLADYL WSLIKNSISQ 450
    RDHVTLLLIV LIKLKDVEGI DTFIQHFDRK GIWNEGVVMD DMDDVTFFYS 500
    DNDFFDLDLI LELMKESDFK RLSYRLAKKY SKDSLIIVDI LLNLLHNPVK 550
    AIKYIKSLPI DETLRCLVTY SKKLLEESPN ETNALLIEVF TGKFKPSTFE 600
    VDLDRRDTTG DFSENIRTVF YSYKTFFNYM NSNGTSDAMS ESSEASHEHE 650
    EPTYHPPKPS IVFSSFVTKP FEFVVFLEAC LACYQQYEGF DEDRQVILTT 700
    LYDLYLNLAQ NDVPERIDDW RSRATGVLRE SNKLVYSAAS NNTSKRVDNS 750
    IMLLISHMDQ SSASAKDKTK IDIASFANDN PEMDLLSTFR AMTLNEEPST 800
    CLKFLEKYGT EEPKLLQVAL SYFVSNKLIF KEMGGNEVLK EKVLRPIIEG 850
    ERMPLLDIIK ALSRTNVAHF GLIQDIIIDH VKTEDTEIKR NEKLIESYDK 900
    ELKEKNKKLK NTINSDQPLH VPLKNQTCFM CRLTLDIPVV FFKCGHIYHQ 950
    HCLNEEEDTL ESERKLFKCP KCLVDLETSN KLFEAQHEVV EKNDLLNFAL 1000
    NSEEGSRDRF KVITEFLGRG AISYSDITI 1029
    Length:1,029
    Mass (Da):117,478
    Last modified:August 1, 1991 - v2
    Checksum:iE5216AD3C285CF34
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti37 – 371A → R in CAA33413. (PubMed:2670552)Curated
    Sequence conflicti619 – 6257VFYSYKT → MFFTVTKH in CAA33413. (PubMed:2670552)Curated
    Sequence conflicti769 – 7702TK → KQ in CAA33413. (PubMed:2670552)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X54466 Genomic DNA. Translation: CAA38348.1.
    X15355 Genomic DNA. Translation: CAA33413.1.
    Z49939 Genomic DNA. Translation: CAA90202.1.
    BK006946 Genomic DNA. Translation: DAA10131.1.
    PIRiS57598.
    RefSeqiNP_013958.1. NM_001182738.1.

    Genome annotation databases

    EnsemblFungiiYMR231W; YMR231W; YMR231W.
    GeneIDi855271.
    KEGGisce:YMR231W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X54466 Genomic DNA. Translation: CAA38348.1 .
    X15355 Genomic DNA. Translation: CAA33413.1 .
    Z49939 Genomic DNA. Translation: CAA90202.1 .
    BK006946 Genomic DNA. Translation: DAA10131.1 .
    PIRi S57598.
    RefSeqi NP_013958.1. NM_001182738.1.

    3D structure databases

    ProteinModelPortali P12868.
    SMRi P12868. Positions 927-977.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35409. 63 interactions.
    DIPi DIP-4409N.
    IntActi P12868. 13 interactions.
    MINTi MINT-545862.
    STRINGi 4932.YMR231W.

    Proteomic databases

    MaxQBi P12868.
    PaxDbi P12868.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YMR231W ; YMR231W ; YMR231W .
    GeneIDi 855271.
    KEGGi sce:YMR231W.

    Organism-specific databases

    CYGDi YMR231w.
    SGDi S000004844. PEP5.

    Phylogenomic databases

    eggNOGi NOG294563.
    GeneTreei ENSGT00390000015058.
    HOGENOMi HOG000074719.
    OMAi GMHEDVL.
    OrthoDBi EOG7GN2W1.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32912-MONOMER.

    Miscellaneous databases

    NextBioi 978885.
    PROi P12868.

    Gene expression databases

    Genevestigatori P12868.

    Family and domain databases

    Gene3Di 2.130.10.10. 1 hit.
    3.30.40.10. 1 hit.
    InterProi IPR000547. Clathrin_H-chain/VPS_repeat.
    IPR016528. VPS11.
    IPR024763. VPS11_C.
    IPR015943. WD40/YVTN_repeat-like_dom.
    IPR017986. WD40_repeat_dom.
    IPR001965. Znf_PHD.
    IPR001841. Znf_RING.
    IPR013083. Znf_RING/FYVE/PHD.
    [Graphical view ]
    Pfami PF12451. VPS11_C. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF007860. VPS11. 1 hit.
    SMARTi SM00249. PHD. 1 hit.
    SM00184. RING. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50978. SSF50978. 1 hit.
    PROSITEi PS50236. CHCR. 1 hit.
    PS50089. ZF_RING_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation and characterization of PEP5, a gene essential for vacuolar biogenesis in Saccharomyces cerevisiae."
      Woolford C.A., Dixon C.K., Manolson M.F., Wright R., Jones E.W.
      Genetics 125:739-752(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Characterization of the END1 gene required for vacuole biogenesis and gluconeogenic growth of budding yeast."
      Dulic V., Riezman H.
      EMBO J. 8:1349-1359(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "Pep3p/Pep5p complex: a putative docking factor at multiple steps of vesicular transport to the vacuole of Saccharomyces cerevisiae."
      Srivastava A., Woolford C.A., Jones E.W.
      Genetics 156:105-122(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH PEP3 AND PEP7.
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. "Purification of active HOPS complex reveals its affinities for phosphoinositides and the SNARE Vam7p."
      Stroupe C., Collins K.M., Fratti R.A., Wickner W.
      EMBO J. 25:1579-1589(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE HOPS COMPLEX, FUNCTION OF THE HOPS COMPLEX, INTERACTION WITH VAM7.
    8. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiPEP5_YEAST
    AccessioniPrimary (citable) accession number: P12868
    Secondary accession number(s): D6W057
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1989
    Last sequence update: August 1, 1991
    Last modified: October 1, 2014
    This is version 138 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1200 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XIII
      Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

    External Data

    Dasty 3