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P12866

- STE6_YEAST

UniProt

P12866 - STE6_YEAST

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Protein
Alpha-factor-transporting ATPase
Gene
STE6, YKL209C
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

STE6 is required in yeast MATA cells for production of A-factor pheromone. STE6 is involved in the transport of the farnesyl-derivation of the A-factor pheromone.

Catalytic activityi

ATP + H2O + alpha-factor(In) = ADP + phosphate + alpha-factor(Out).

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi392 – 3998ATP 1 Reviewed prediction
Nucleotide bindingi1087 – 10948ATP 2 Reviewed prediction

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. peptide-transporting ATPase activity Source: SGD
  3. protein binding Source: IntAct

GO - Biological processi

  1. peptide pheromone export Source: SGD
  2. response to pheromone Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Pheromone response, Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31968-MONOMER.
BRENDAi3.6.3.48. 984.

Protein family/group databases

TCDBi3.A.1.206.1. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-factor-transporting ATPase (EC:3.6.3.48)
Alternative name(s):
Mating factor A secretion protein STE6
Multiple drug resistance protein homolog
P-glycoprotein
Gene namesi
Name:STE6
Ordered Locus Names:YKL209C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XI

Organism-specific databases

CYGDiYKL209c.
SGDiS000001692. STE6.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2525Cytoplasmic Inferred
Add
BLAST
Transmembranei26 – 4621Helical; Inferred
Add
BLAST
Topological domaini47 – 7529Extracellular Inferred
Add
BLAST
Transmembranei76 – 9621Helical; Inferred
Add
BLAST
Topological domaini97 – 15054Cytoplasmic Inferred
Add
BLAST
Transmembranei151 – 17121Helical; Inferred
Add
BLAST
Topological domaini172 – 1732Extracellular Inferred
Transmembranei174 – 19421Helical; Inferred
Add
BLAST
Topological domaini195 – 26268Cytoplasmic Inferred
Add
BLAST
Transmembranei263 – 28321Helical; Inferred
Add
BLAST
Topological domaini284 – 29613Extracellular Inferred
Add
BLAST
Transmembranei297 – 31721Helical; Inferred
Add
BLAST
Topological domaini318 – 715398Cytoplasmic Inferred
Add
BLAST
Transmembranei716 – 73621Helical; Inferred
Add
BLAST
Topological domaini737 – 76327Extracellular Inferred
Add
BLAST
Transmembranei764 – 78421Helical; Inferred
Add
BLAST
Topological domaini785 – 83854Cytoplasmic Inferred
Add
BLAST
Transmembranei839 – 85921Helical; Inferred
Add
BLAST
Topological domaini860 – 8656Extracellular Inferred
Transmembranei866 – 88621Helical; Inferred
Add
BLAST
Topological domaini887 – 94559Cytoplasmic Inferred
Add
BLAST
Transmembranei946 – 96621Helical; Inferred
Add
BLAST
Topological domaini967 – 98115Extracellular Inferred
Add
BLAST
Transmembranei982 – 100221Helical; Inferred
Add
BLAST
Topological domaini1003 – 1290288Cytoplasmic Inferred
Add
BLAST

GO - Cellular componenti

  1. Golgi apparatus Source: SGD
  2. integral component of membrane Source: UniProtKB-KW
  3. mating projection tip Source: SGD
  4. plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi392 – 3921G → V: 0.8% mating activity.
Mutagenesisi398 – 3981K → A: 25% mating activity.
Mutagenesisi398 – 3981K → R: 1% mating activity.
Mutagenesisi509 – 5091G → D: 0.5% mating activity.
Mutagenesisi1087 – 10871G → V: 0.3% mating activity.
Mutagenesisi1093 – 10931K → A: 26% mating activity.
Mutagenesisi1093 – 10931K → R: 15% mating activity.
Mutagenesisi1193 – 11931G → D: 6% mating activity.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12901290Alpha-factor-transporting ATPase
PRO_0000093370Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi61 – 611N-linked (GlcNAc...) Reviewed prediction
Cross-linki1022 – 1022Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication

Post-translational modificationi

Degraded via the ubiquitin system.

Keywords - PTMi

Glycoprotein, Isopeptide bond, Ubl conjugation

Proteomic databases

MaxQBiP12866.
PaxDbiP12866.
PeptideAtlasiP12866.

Expressioni

Gene expression databases

GenevestigatoriP12866.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
SSM4P403182EBI-18383,EBI-18208

Protein-protein interaction databases

BioGridi33956. 24 interactions.
DIPiDIP-2594N.
IntActiP12866. 9 interactions.
MINTiMINT-427396.
STRINGi4932.YKL209C.

Structurei

3D structure databases

ProteinModelPortaliP12866.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini27 – 319293ABC transmembrane type-1 1
Add
BLAST
Domaini357 – 603247ABC transporter 1
Add
BLAST
Domaini717 – 1007291ABC transmembrane type-1 2
Add
BLAST
Domaini1052 – 1287236ABC transporter 2
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1132.
GeneTreeiENSGT00530000062896.
HOGENOMiHOG000093959.
KOiK05658.
OMAiMAWITHI.
OrthoDBiEOG70KGXV.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR001140. ABC_transptr_TM_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00664. ABC_membrane. 2 hits.
PF00005. ABC_tran. 2 hits.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF90123. SSF90123. 2 hits.
PROSITEiPS50929. ABC_TM1F. 2 hits.
PS00211. ABC_TRANSPORTER_1. 2 hits.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P12866-1 [UniParc]FASTAAdd to Basket

« Hide

MNFLSFKTTK HYHIFRYVNI RNDYRLLMIM IIGTVATGLV PAITSILTGR     50
VFDLLSVFVA NGSHQGLYSQ LVQRSMAVMA LGAASVPVMW LSLTSWMHIG 100
ERQGFRIRSQ ILEAYLEEKP MEWYDNNEKL LGDFTQINRC VEELRSSSAE 150
ASAITFQNLV AICALLGTSF YYSWSLTLII LCSSPIITFF AVVFSRMIHV 200
YSEKENSETS KAAQLLTWSM NAAQLVRLYC TQRLERKKFK EIILNCNTFF 250
IKSCFFVAAN AGILRFLTLT MFVQGFWFGS AMIKKGKLNI NDVITCFHSC 300
IMLGSTLNNT LHQIVVLQKG GVAMEKIMTL LKDGSKRNPL NKTVAHQFPL 350
DYATSDLTFA NVSFSYPSRP SEAVLKNVSL NFSAGQFTFI VGKSGSGKST 400
LSNLLLRFYD GYNGSISING HNIQTIDQKL LIENITVVEQ RCTLFNDTLR 450
KNILLGSTDS VRNADCSTNE NRHLIKDACQ MALLDRFILD LPDGLETLIG 500
TGGVTLSGGQ QQRVAIARAF IRDTPILFLD EAVSALDIVH RNLLMKAIRH 550
WRKGKTTIIL THELSQIESD DYLYLMKEGE VVESGTQSEL LADPTTTFST 600
WYHLQNDYSD AKTIVDTETE EKSIHTVESF NSQLETPKLG SCLSNLGYDE 650
TDQLSFYEAI YQKRSNVRTR RVKVEEENIG YALKQQKNTE SSTGPQLLSI 700
IQIIKRMIKS IRYKKILILG LLCSLIAGAT NPVFSYTFSF LLEGIVPSTD 750
GKTGSSHYLA KWSLLVLGVA AADGIFNFAK GFLLDCCSEY WVMDLRNEVM 800
EKLTRKNMDW FSGENNKASE ISALVLNDLR DLRSLVSEFL SAMTSFVTVS 850
TIGLIWALVS GWKLSLVCIS MFPLIIIFSA IYGGILQKCE TDYKTSVAQL 900
ENCLYQIVTN IKTIKCLQAE FHFQLTYHDL KIKMQQIASK RAIATGFGIS 950
MTNMIVMCIQ AIIYYYGLKL VMIHEYTSKE MFTTFTLLLF TIMSCTSLVS 1000
QIPDISRGQR AASWIYRILD EKHNTLEVEN NNARTVGIAG HTYHGKEKKP 1050
IVSIQNLTFA YPSAPTAFVY KNMNFDMFCG QTLGIIGESG TGKSTLVLLL 1100
TKLYNCEVGK IKIDGTDVND WNLTSLRKEI SVVEQKPLLF NGTIRDNLTY 1150
GLQDEILEIE MYDALKYVGI HDFVISSPQG LDTRIDTTLL SGGQAQRLCI 1200
ARALLRKSKI LILDECTSAL DSVSSSIINE IVKKGPPALL TMVITHSEQM 1250
MRSCNSIAVL KDGKVVERGN FDTLYNNRGE LFQIVSNQSS 1290
Length:1,290
Mass (Da):144,766
Last modified:October 1, 1989 - v1
Checksum:iB240B7E51D9680D5
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X15428 Genomic DNA. Translation: CAA33467.1.
M26376 Genomic DNA. Translation: AAA35116.1.
Z28209 Genomic DNA. Translation: CAA82054.1.
M12842 Genomic DNA. Translation: AAA35117.1.
BK006944 Genomic DNA. Translation: DAA08960.1.
PIRiS05789. DVBYS6.
RefSeqiNP_012713.1. NM_001179774.1.

Genome annotation databases

EnsemblFungiiYKL209C; YKL209C; YKL209C.
GeneIDi853671.
KEGGisce:YKL209C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X15428 Genomic DNA. Translation: CAA33467.1 .
M26376 Genomic DNA. Translation: AAA35116.1 .
Z28209 Genomic DNA. Translation: CAA82054.1 .
M12842 Genomic DNA. Translation: AAA35117.1 .
BK006944 Genomic DNA. Translation: DAA08960.1 .
PIRi S05789. DVBYS6.
RefSeqi NP_012713.1. NM_001179774.1.

3D structure databases

ProteinModelPortali P12866.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33956. 24 interactions.
DIPi DIP-2594N.
IntActi P12866. 9 interactions.
MINTi MINT-427396.
STRINGi 4932.YKL209C.

Protein family/group databases

TCDBi 3.A.1.206.1. the atp-binding cassette (abc) superfamily.

Proteomic databases

MaxQBi P12866.
PaxDbi P12866.
PeptideAtlasi P12866.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YKL209C ; YKL209C ; YKL209C .
GeneIDi 853671.
KEGGi sce:YKL209C.

Organism-specific databases

CYGDi YKL209c.
SGDi S000001692. STE6.

Phylogenomic databases

eggNOGi COG1132.
GeneTreei ENSGT00530000062896.
HOGENOMi HOG000093959.
KOi K05658.
OMAi MAWITHI.
OrthoDBi EOG70KGXV.

Enzyme and pathway databases

BioCyci YEAST:G3O-31968-MONOMER.
BRENDAi 3.6.3.48. 984.

Miscellaneous databases

NextBioi 974617.

Gene expression databases

Genevestigatori P12866.

Family and domain databases

Gene3Di 3.40.50.300. 2 hits.
InterProi IPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR001140. ABC_transptr_TM_dom.
IPR027417. P-loop_NTPase.
[Graphical view ]
Pfami PF00664. ABC_membrane. 2 hits.
PF00005. ABC_tran. 2 hits.
[Graphical view ]
SMARTi SM00382. AAA. 2 hits.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 2 hits.
SSF90123. SSF90123. 2 hits.
PROSITEi PS50929. ABC_TM1F. 2 hits.
PS00211. ABC_TRANSPORTER_1. 2 hits.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The yeast STE6 gene encodes a homologue of the mammalian multidrug resistance P-glycoprotein."
    McGrath J.P., Varshavsky A.
    Nature 340:400-404(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "Saccharomyces cerevisiae STE6 gene product: a novel pathway for protein export in eukaryotic cells."
    Kuchler K., Sterne R.E., Thorner J.W.
    EMBO J. 8:3973-3984(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Complete DNA sequence of yeast chromosome XI."
    Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
    , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
    Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "Sequences upstream of the STE6 gene required for its expression and regulation by the mating type locus in Saccharomyces cerevisiae."
    Wilson K.L., Herskowitz I.
    Proc. Natl. Acad. Sci. U.S.A. 83:2536-2540(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-41.
  6. "Mutational analysis of the yeast a-factor transporter STE6, a member of the ATP binding cassette (ABC) protein superfamily."
    Berkover C., Michaelis S.
    EMBO J. 10:3777-3785(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS.
  7. "The ABC-transporter Ste6 accumulates in the plasma membrane in a ubiquitinated form in endocytosis mutants."
    Koelling R., Hollenberg C.
    EMBO J. 13:3261-3271(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEGRADATION BY UBIQUITINATION.
  8. "Comparative topology studies in Saccharomyces cerevisiae and in Escherichia coli. The N-terminal half of the yeast ABC protein Ste6."
    Geller D., Taglicht D., Edgar R., Tam A., Pines O., Michaelis S., Bibi E.
    J. Biol. Chem. 271:13746-13753(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY.
  9. Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-1022.
    Strain: SUB592.
  10. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.

Entry informationi

Entry nameiSTE6_YEAST
AccessioniPrimary (citable) accession number: P12866
Secondary accession number(s): D6VWZ4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 1, 1989
Last modified: June 11, 2014
This is version 146 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

External Data

Dasty 3

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