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P12866

- STE6_YEAST

UniProt

P12866 - STE6_YEAST

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Protein

Alpha-factor-transporting ATPase

Gene

STE6

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

STE6 is required in yeast MATA cells for production of A-factor pheromone. STE6 is involved in the transport of the farnesyl-derivation of the A-factor pheromone.

Catalytic activityi

ATP + H2O + alpha-factor(In) = ADP + phosphate + alpha-factor(Out).

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi392 – 3998ATP 1PROSITE-ProRule annotation
Nucleotide bindingi1087 – 10948ATP 2PROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. peptide-transporting ATPase activity Source: SGD

GO - Biological processi

  1. peptide pheromone export Source: SGD
  2. response to pheromone Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Pheromone response, Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31968-MONOMER.
BRENDAi3.6.3.48. 984.
ReactomeiREACT_188984. PPARA activates gene expression.
REACT_221494. Abacavir transmembrane transport.
REACT_232789. ABC-family proteins mediated transport.
REACT_257005. Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol.
REACT_269909. Recycling of bile acids and salts.

Protein family/group databases

TCDBi3.A.1.206.1. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-factor-transporting ATPase (EC:3.6.3.48)
Alternative name(s):
Mating factor A secretion protein STE6
Multiple drug resistance protein homolog
P-glycoprotein
Gene namesi
Name:STE6
Ordered Locus Names:YKL209C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XI

Organism-specific databases

CYGDiYKL209c.
SGDiS000001692. STE6.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2525CytoplasmicCuratedAdd
BLAST
Transmembranei26 – 4621HelicalCuratedAdd
BLAST
Topological domaini47 – 7529ExtracellularCuratedAdd
BLAST
Transmembranei76 – 9621HelicalCuratedAdd
BLAST
Topological domaini97 – 15054CytoplasmicCuratedAdd
BLAST
Transmembranei151 – 17121HelicalCuratedAdd
BLAST
Topological domaini172 – 1732ExtracellularCurated
Transmembranei174 – 19421HelicalCuratedAdd
BLAST
Topological domaini195 – 26268CytoplasmicCuratedAdd
BLAST
Transmembranei263 – 28321HelicalCuratedAdd
BLAST
Topological domaini284 – 29613ExtracellularCuratedAdd
BLAST
Transmembranei297 – 31721HelicalCuratedAdd
BLAST
Topological domaini318 – 715398CytoplasmicCuratedAdd
BLAST
Transmembranei716 – 73621HelicalCuratedAdd
BLAST
Topological domaini737 – 76327ExtracellularCuratedAdd
BLAST
Transmembranei764 – 78421HelicalCuratedAdd
BLAST
Topological domaini785 – 83854CytoplasmicCuratedAdd
BLAST
Transmembranei839 – 85921HelicalCuratedAdd
BLAST
Topological domaini860 – 8656ExtracellularCurated
Transmembranei866 – 88621HelicalCuratedAdd
BLAST
Topological domaini887 – 94559CytoplasmicCuratedAdd
BLAST
Transmembranei946 – 96621HelicalCuratedAdd
BLAST
Topological domaini967 – 98115ExtracellularCuratedAdd
BLAST
Transmembranei982 – 100221HelicalCuratedAdd
BLAST
Topological domaini1003 – 1290288CytoplasmicCuratedAdd
BLAST

GO - Cellular componenti

  1. Golgi apparatus Source: SGD
  2. integral component of membrane Source: UniProtKB-KW
  3. mating projection tip Source: SGD
  4. plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi392 – 3921G → V: 0.8% mating activity. 1 Publication
Mutagenesisi398 – 3981K → A: 25% mating activity. 1 Publication
Mutagenesisi398 – 3981K → R: 1% mating activity. 1 Publication
Mutagenesisi509 – 5091G → D: 0.5% mating activity. 1 Publication
Mutagenesisi1087 – 10871G → V: 0.3% mating activity. 1 Publication
Mutagenesisi1093 – 10931K → A: 26% mating activity. 1 Publication
Mutagenesisi1093 – 10931K → R: 15% mating activity. 1 Publication
Mutagenesisi1193 – 11931G → D: 6% mating activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12901290Alpha-factor-transporting ATPasePRO_0000093370Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi61 – 611N-linked (GlcNAc...)Sequence Analysis
Cross-linki1022 – 1022Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication

Post-translational modificationi

Degraded via the ubiquitin system.1 Publication

Keywords - PTMi

Glycoprotein, Isopeptide bond, Ubl conjugation

Proteomic databases

MaxQBiP12866.
PaxDbiP12866.
PeptideAtlasiP12866.

Expressioni

Gene expression databases

GenevestigatoriP12866.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
SSM4P403182EBI-18383,EBI-18208

Protein-protein interaction databases

BioGridi33956. 24 interactions.
DIPiDIP-2594N.
IntActiP12866. 9 interactions.
MINTiMINT-427396.
STRINGi4932.YKL209C.

Structurei

3D structure databases

ProteinModelPortaliP12866.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini27 – 319293ABC transmembrane type-1 1PROSITE-ProRule annotationAdd
BLAST
Domaini357 – 603247ABC transporter 1PROSITE-ProRule annotationAdd
BLAST
Domaini717 – 1007291ABC transmembrane type-1 2PROSITE-ProRule annotationAdd
BLAST
Domaini1052 – 1287236ABC transporter 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 2 ABC transmembrane type-1 domains.PROSITE-ProRule annotation
Contains 2 ABC transporter domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1132.
GeneTreeiENSGT00530000062896.
HOGENOMiHOG000093959.
InParanoidiP12866.
KOiK05658.
OMAiMAWITHI.
OrthoDBiEOG70KGXV.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR001140. ABC_transptr_TM_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00664. ABC_membrane. 2 hits.
PF00005. ABC_tran. 2 hits.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF90123. SSF90123. 2 hits.
PROSITEiPS50929. ABC_TM1F. 2 hits.
PS00211. ABC_TRANSPORTER_1. 2 hits.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P12866-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNFLSFKTTK HYHIFRYVNI RNDYRLLMIM IIGTVATGLV PAITSILTGR
60 70 80 90 100
VFDLLSVFVA NGSHQGLYSQ LVQRSMAVMA LGAASVPVMW LSLTSWMHIG
110 120 130 140 150
ERQGFRIRSQ ILEAYLEEKP MEWYDNNEKL LGDFTQINRC VEELRSSSAE
160 170 180 190 200
ASAITFQNLV AICALLGTSF YYSWSLTLII LCSSPIITFF AVVFSRMIHV
210 220 230 240 250
YSEKENSETS KAAQLLTWSM NAAQLVRLYC TQRLERKKFK EIILNCNTFF
260 270 280 290 300
IKSCFFVAAN AGILRFLTLT MFVQGFWFGS AMIKKGKLNI NDVITCFHSC
310 320 330 340 350
IMLGSTLNNT LHQIVVLQKG GVAMEKIMTL LKDGSKRNPL NKTVAHQFPL
360 370 380 390 400
DYATSDLTFA NVSFSYPSRP SEAVLKNVSL NFSAGQFTFI VGKSGSGKST
410 420 430 440 450
LSNLLLRFYD GYNGSISING HNIQTIDQKL LIENITVVEQ RCTLFNDTLR
460 470 480 490 500
KNILLGSTDS VRNADCSTNE NRHLIKDACQ MALLDRFILD LPDGLETLIG
510 520 530 540 550
TGGVTLSGGQ QQRVAIARAF IRDTPILFLD EAVSALDIVH RNLLMKAIRH
560 570 580 590 600
WRKGKTTIIL THELSQIESD DYLYLMKEGE VVESGTQSEL LADPTTTFST
610 620 630 640 650
WYHLQNDYSD AKTIVDTETE EKSIHTVESF NSQLETPKLG SCLSNLGYDE
660 670 680 690 700
TDQLSFYEAI YQKRSNVRTR RVKVEEENIG YALKQQKNTE SSTGPQLLSI
710 720 730 740 750
IQIIKRMIKS IRYKKILILG LLCSLIAGAT NPVFSYTFSF LLEGIVPSTD
760 770 780 790 800
GKTGSSHYLA KWSLLVLGVA AADGIFNFAK GFLLDCCSEY WVMDLRNEVM
810 820 830 840 850
EKLTRKNMDW FSGENNKASE ISALVLNDLR DLRSLVSEFL SAMTSFVTVS
860 870 880 890 900
TIGLIWALVS GWKLSLVCIS MFPLIIIFSA IYGGILQKCE TDYKTSVAQL
910 920 930 940 950
ENCLYQIVTN IKTIKCLQAE FHFQLTYHDL KIKMQQIASK RAIATGFGIS
960 970 980 990 1000
MTNMIVMCIQ AIIYYYGLKL VMIHEYTSKE MFTTFTLLLF TIMSCTSLVS
1010 1020 1030 1040 1050
QIPDISRGQR AASWIYRILD EKHNTLEVEN NNARTVGIAG HTYHGKEKKP
1060 1070 1080 1090 1100
IVSIQNLTFA YPSAPTAFVY KNMNFDMFCG QTLGIIGESG TGKSTLVLLL
1110 1120 1130 1140 1150
TKLYNCEVGK IKIDGTDVND WNLTSLRKEI SVVEQKPLLF NGTIRDNLTY
1160 1170 1180 1190 1200
GLQDEILEIE MYDALKYVGI HDFVISSPQG LDTRIDTTLL SGGQAQRLCI
1210 1220 1230 1240 1250
ARALLRKSKI LILDECTSAL DSVSSSIINE IVKKGPPALL TMVITHSEQM
1260 1270 1280 1290
MRSCNSIAVL KDGKVVERGN FDTLYNNRGE LFQIVSNQSS
Length:1,290
Mass (Da):144,766
Last modified:October 1, 1989 - v1
Checksum:iB240B7E51D9680D5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15428 Genomic DNA. Translation: CAA33467.1.
M26376 Genomic DNA. Translation: AAA35116.1.
Z28209 Genomic DNA. Translation: CAA82054.1.
M12842 Genomic DNA. Translation: AAA35117.1.
BK006944 Genomic DNA. Translation: DAA08960.1.
PIRiS05789. DVBYS6.
RefSeqiNP_012713.1. NM_001179774.1.

Genome annotation databases

EnsemblFungiiYKL209C; YKL209C; YKL209C.
GeneIDi853671.
KEGGisce:YKL209C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15428 Genomic DNA. Translation: CAA33467.1 .
M26376 Genomic DNA. Translation: AAA35116.1 .
Z28209 Genomic DNA. Translation: CAA82054.1 .
M12842 Genomic DNA. Translation: AAA35117.1 .
BK006944 Genomic DNA. Translation: DAA08960.1 .
PIRi S05789. DVBYS6.
RefSeqi NP_012713.1. NM_001179774.1.

3D structure databases

ProteinModelPortali P12866.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33956. 24 interactions.
DIPi DIP-2594N.
IntActi P12866. 9 interactions.
MINTi MINT-427396.
STRINGi 4932.YKL209C.

Protein family/group databases

TCDBi 3.A.1.206.1. the atp-binding cassette (abc) superfamily.

Proteomic databases

MaxQBi P12866.
PaxDbi P12866.
PeptideAtlasi P12866.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YKL209C ; YKL209C ; YKL209C .
GeneIDi 853671.
KEGGi sce:YKL209C.

Organism-specific databases

CYGDi YKL209c.
SGDi S000001692. STE6.

Phylogenomic databases

eggNOGi COG1132.
GeneTreei ENSGT00530000062896.
HOGENOMi HOG000093959.
InParanoidi P12866.
KOi K05658.
OMAi MAWITHI.
OrthoDBi EOG70KGXV.

Enzyme and pathway databases

BioCyci YEAST:G3O-31968-MONOMER.
BRENDAi 3.6.3.48. 984.
Reactomei REACT_188984. PPARA activates gene expression.
REACT_221494. Abacavir transmembrane transport.
REACT_232789. ABC-family proteins mediated transport.
REACT_257005. Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol.
REACT_269909. Recycling of bile acids and salts.

Miscellaneous databases

NextBioi 974617.

Gene expression databases

Genevestigatori P12866.

Family and domain databases

Gene3Di 3.40.50.300. 2 hits.
InterProi IPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR001140. ABC_transptr_TM_dom.
IPR027417. P-loop_NTPase.
[Graphical view ]
Pfami PF00664. ABC_membrane. 2 hits.
PF00005. ABC_tran. 2 hits.
[Graphical view ]
SMARTi SM00382. AAA. 2 hits.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 2 hits.
SSF90123. SSF90123. 2 hits.
PROSITEi PS50929. ABC_TM1F. 2 hits.
PS00211. ABC_TRANSPORTER_1. 2 hits.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The yeast STE6 gene encodes a homologue of the mammalian multidrug resistance P-glycoprotein."
    McGrath J.P., Varshavsky A.
    Nature 340:400-404(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "Saccharomyces cerevisiae STE6 gene product: a novel pathway for protein export in eukaryotic cells."
    Kuchler K., Sterne R.E., Thorner J.W.
    EMBO J. 8:3973-3984(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Complete DNA sequence of yeast chromosome XI."
    Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
    , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
    Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "Sequences upstream of the STE6 gene required for its expression and regulation by the mating type locus in Saccharomyces cerevisiae."
    Wilson K.L., Herskowitz I.
    Proc. Natl. Acad. Sci. U.S.A. 83:2536-2540(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-41.
  6. "Mutational analysis of the yeast a-factor transporter STE6, a member of the ATP binding cassette (ABC) protein superfamily."
    Berkover C., Michaelis S.
    EMBO J. 10:3777-3785(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS.
  7. "The ABC-transporter Ste6 accumulates in the plasma membrane in a ubiquitinated form in endocytosis mutants."
    Koelling R., Hollenberg C.
    EMBO J. 13:3261-3271(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEGRADATION BY UBIQUITINATION.
  8. "Comparative topology studies in Saccharomyces cerevisiae and in Escherichia coli. The N-terminal half of the yeast ABC protein Ste6."
    Geller D., Taglicht D., Edgar R., Tam A., Pines O., Michaelis S., Bibi E.
    J. Biol. Chem. 271:13746-13753(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY.
  9. Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-1022.
    Strain: SUB592.
  10. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.

Entry informationi

Entry nameiSTE6_YEAST
AccessioniPrimary (citable) accession number: P12866
Secondary accession number(s): D6VWZ4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 1, 1989
Last modified: November 26, 2014
This is version 149 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

External Data

Dasty 3