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Protein

Alpha-factor-transporting ATPase

Gene

STE6

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

STE6 is required in yeast MATA cells for production of A-factor pheromone. STE6 is involved in the transport of the farnesyl-derivation of the A-factor pheromone.

Catalytic activityi

ATP + H2O + alpha-factor(In) = ADP + phosphate + alpha-factor(Out).

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi392 – 399ATP 1PROSITE-ProRule annotation8
Nucleotide bindingi1087 – 1094ATP 2PROSITE-ProRule annotation8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • peptide-transporting ATPase activity Source: SGD

GO - Biological processi

  • peptide pheromone export Source: SGD
  • response to pheromone Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Pheromone response, Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31968-MONOMER.
BRENDAi3.6.3.48. 984.
ReactomeiR-SCE-159418. Recycling of bile acids and salts.
R-SCE-193368. Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol.
R-SCE-382556. ABC-family proteins mediated transport.

Protein family/group databases

TCDBi3.A.1.206.1. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-factor-transporting ATPase (EC:3.6.3.48)
Alternative name(s):
Mating factor A secretion protein STE6
Multiple drug resistance protein homolog
P-glycoprotein
Gene namesi
Name:STE6
Ordered Locus Names:YKL209C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XI

Organism-specific databases

EuPathDBiFungiDB:YKL209C.
SGDiS000001692. STE6.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 25CytoplasmicCuratedAdd BLAST25
Transmembranei26 – 46HelicalCuratedAdd BLAST21
Topological domaini47 – 75ExtracellularCuratedAdd BLAST29
Transmembranei76 – 96HelicalCuratedAdd BLAST21
Topological domaini97 – 150CytoplasmicCuratedAdd BLAST54
Transmembranei151 – 171HelicalCuratedAdd BLAST21
Topological domaini172 – 173ExtracellularCurated2
Transmembranei174 – 194HelicalCuratedAdd BLAST21
Topological domaini195 – 262CytoplasmicCuratedAdd BLAST68
Transmembranei263 – 283HelicalCuratedAdd BLAST21
Topological domaini284 – 296ExtracellularCuratedAdd BLAST13
Transmembranei297 – 317HelicalCuratedAdd BLAST21
Topological domaini318 – 715CytoplasmicCuratedAdd BLAST398
Transmembranei716 – 736HelicalCuratedAdd BLAST21
Topological domaini737 – 763ExtracellularCuratedAdd BLAST27
Transmembranei764 – 784HelicalCuratedAdd BLAST21
Topological domaini785 – 838CytoplasmicCuratedAdd BLAST54
Transmembranei839 – 859HelicalCuratedAdd BLAST21
Topological domaini860 – 865ExtracellularCurated6
Transmembranei866 – 886HelicalCuratedAdd BLAST21
Topological domaini887 – 945CytoplasmicCuratedAdd BLAST59
Transmembranei946 – 966HelicalCuratedAdd BLAST21
Topological domaini967 – 981ExtracellularCuratedAdd BLAST15
Transmembranei982 – 1002HelicalCuratedAdd BLAST21
Topological domaini1003 – 1290CytoplasmicCuratedAdd BLAST288

GO - Cellular componenti

  • Golgi apparatus Source: SGD
  • integral component of membrane Source: UniProtKB-KW
  • mating projection tip Source: SGD
  • plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi392G → V: 0.8% mating activity. 1 Publication1
Mutagenesisi398K → A: 25% mating activity. 1 Publication1
Mutagenesisi398K → R: 1% mating activity. 1 Publication1
Mutagenesisi509G → D: 0.5% mating activity. 1 Publication1
Mutagenesisi1087G → V: 0.3% mating activity. 1 Publication1
Mutagenesisi1093K → A: 26% mating activity. 1 Publication1
Mutagenesisi1093K → R: 15% mating activity. 1 Publication1
Mutagenesisi1193G → D: 6% mating activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000933701 – 1290Alpha-factor-transporting ATPaseAdd BLAST1290

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi61N-linked (GlcNAc...)Sequence analysis1
Cross-linki1022Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication

Post-translational modificationi

Degraded via the ubiquitin system.1 Publication

Keywords - PTMi

Glycoprotein, Isopeptide bond, Ubl conjugation

Proteomic databases

MaxQBiP12866.
PRIDEiP12866.

PTM databases

iPTMnetiP12866.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
SSM4P403182EBI-18383,EBI-18208

Protein-protein interaction databases

BioGridi33956. 25 interactors.
DIPiDIP-2594N.
IntActiP12866. 9 interactors.
MINTiMINT-427396.

Structurei

3D structure databases

ProteinModelPortaliP12866.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini27 – 319ABC transmembrane type-1 1PROSITE-ProRule annotationAdd BLAST293
Domaini357 – 603ABC transporter 1PROSITE-ProRule annotationAdd BLAST247
Domaini717 – 1007ABC transmembrane type-1 2PROSITE-ProRule annotationAdd BLAST291
Domaini1052 – 1287ABC transporter 2PROSITE-ProRule annotationAdd BLAST236

Sequence similaritiesi

Contains 2 ABC transmembrane type-1 domains.PROSITE-ProRule annotation
Contains 2 ABC transporter domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00530000062896.
HOGENOMiHOG000093959.
InParanoidiP12866.
KOiK05658.
OMAiNINWIRN.
OrthoDBiEOG092C02C4.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
IPR030246. Ste6/Hst6.
[Graphical view]
PANTHERiPTHR24223:SF183. PTHR24223:SF183. 2 hits.
PfamiPF00664. ABC_membrane. 2 hits.
PF00005. ABC_tran. 2 hits.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF90123. SSF90123. 2 hits.
PROSITEiPS50929. ABC_TM1F. 2 hits.
PS00211. ABC_TRANSPORTER_1. 2 hits.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P12866-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNFLSFKTTK HYHIFRYVNI RNDYRLLMIM IIGTVATGLV PAITSILTGR
60 70 80 90 100
VFDLLSVFVA NGSHQGLYSQ LVQRSMAVMA LGAASVPVMW LSLTSWMHIG
110 120 130 140 150
ERQGFRIRSQ ILEAYLEEKP MEWYDNNEKL LGDFTQINRC VEELRSSSAE
160 170 180 190 200
ASAITFQNLV AICALLGTSF YYSWSLTLII LCSSPIITFF AVVFSRMIHV
210 220 230 240 250
YSEKENSETS KAAQLLTWSM NAAQLVRLYC TQRLERKKFK EIILNCNTFF
260 270 280 290 300
IKSCFFVAAN AGILRFLTLT MFVQGFWFGS AMIKKGKLNI NDVITCFHSC
310 320 330 340 350
IMLGSTLNNT LHQIVVLQKG GVAMEKIMTL LKDGSKRNPL NKTVAHQFPL
360 370 380 390 400
DYATSDLTFA NVSFSYPSRP SEAVLKNVSL NFSAGQFTFI VGKSGSGKST
410 420 430 440 450
LSNLLLRFYD GYNGSISING HNIQTIDQKL LIENITVVEQ RCTLFNDTLR
460 470 480 490 500
KNILLGSTDS VRNADCSTNE NRHLIKDACQ MALLDRFILD LPDGLETLIG
510 520 530 540 550
TGGVTLSGGQ QQRVAIARAF IRDTPILFLD EAVSALDIVH RNLLMKAIRH
560 570 580 590 600
WRKGKTTIIL THELSQIESD DYLYLMKEGE VVESGTQSEL LADPTTTFST
610 620 630 640 650
WYHLQNDYSD AKTIVDTETE EKSIHTVESF NSQLETPKLG SCLSNLGYDE
660 670 680 690 700
TDQLSFYEAI YQKRSNVRTR RVKVEEENIG YALKQQKNTE SSTGPQLLSI
710 720 730 740 750
IQIIKRMIKS IRYKKILILG LLCSLIAGAT NPVFSYTFSF LLEGIVPSTD
760 770 780 790 800
GKTGSSHYLA KWSLLVLGVA AADGIFNFAK GFLLDCCSEY WVMDLRNEVM
810 820 830 840 850
EKLTRKNMDW FSGENNKASE ISALVLNDLR DLRSLVSEFL SAMTSFVTVS
860 870 880 890 900
TIGLIWALVS GWKLSLVCIS MFPLIIIFSA IYGGILQKCE TDYKTSVAQL
910 920 930 940 950
ENCLYQIVTN IKTIKCLQAE FHFQLTYHDL KIKMQQIASK RAIATGFGIS
960 970 980 990 1000
MTNMIVMCIQ AIIYYYGLKL VMIHEYTSKE MFTTFTLLLF TIMSCTSLVS
1010 1020 1030 1040 1050
QIPDISRGQR AASWIYRILD EKHNTLEVEN NNARTVGIAG HTYHGKEKKP
1060 1070 1080 1090 1100
IVSIQNLTFA YPSAPTAFVY KNMNFDMFCG QTLGIIGESG TGKSTLVLLL
1110 1120 1130 1140 1150
TKLYNCEVGK IKIDGTDVND WNLTSLRKEI SVVEQKPLLF NGTIRDNLTY
1160 1170 1180 1190 1200
GLQDEILEIE MYDALKYVGI HDFVISSPQG LDTRIDTTLL SGGQAQRLCI
1210 1220 1230 1240 1250
ARALLRKSKI LILDECTSAL DSVSSSIINE IVKKGPPALL TMVITHSEQM
1260 1270 1280 1290
MRSCNSIAVL KDGKVVERGN FDTLYNNRGE LFQIVSNQSS
Length:1,290
Mass (Da):144,766
Last modified:October 1, 1989 - v1
Checksum:iB240B7E51D9680D5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15428 Genomic DNA. Translation: CAA33467.1.
M26376 Genomic DNA. Translation: AAA35116.1.
Z28209 Genomic DNA. Translation: CAA82054.1.
M12842 Genomic DNA. Translation: AAA35117.1.
BK006944 Genomic DNA. Translation: DAA08960.1.
PIRiS05789. DVBYS6.
RefSeqiNP_012713.1. NM_001179774.1.

Genome annotation databases

EnsemblFungiiYKL209C; YKL209C; YKL209C.
GeneIDi853671.
KEGGisce:YKL209C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15428 Genomic DNA. Translation: CAA33467.1.
M26376 Genomic DNA. Translation: AAA35116.1.
Z28209 Genomic DNA. Translation: CAA82054.1.
M12842 Genomic DNA. Translation: AAA35117.1.
BK006944 Genomic DNA. Translation: DAA08960.1.
PIRiS05789. DVBYS6.
RefSeqiNP_012713.1. NM_001179774.1.

3D structure databases

ProteinModelPortaliP12866.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33956. 25 interactors.
DIPiDIP-2594N.
IntActiP12866. 9 interactors.
MINTiMINT-427396.

Protein family/group databases

TCDBi3.A.1.206.1. the atp-binding cassette (abc) superfamily.

PTM databases

iPTMnetiP12866.

Proteomic databases

MaxQBiP12866.
PRIDEiP12866.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYKL209C; YKL209C; YKL209C.
GeneIDi853671.
KEGGisce:YKL209C.

Organism-specific databases

EuPathDBiFungiDB:YKL209C.
SGDiS000001692. STE6.

Phylogenomic databases

GeneTreeiENSGT00530000062896.
HOGENOMiHOG000093959.
InParanoidiP12866.
KOiK05658.
OMAiNINWIRN.
OrthoDBiEOG092C02C4.

Enzyme and pathway databases

BioCyciYEAST:G3O-31968-MONOMER.
BRENDAi3.6.3.48. 984.
ReactomeiR-SCE-159418. Recycling of bile acids and salts.
R-SCE-193368. Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol.
R-SCE-382556. ABC-family proteins mediated transport.

Miscellaneous databases

PROiP12866.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
IPR030246. Ste6/Hst6.
[Graphical view]
PANTHERiPTHR24223:SF183. PTHR24223:SF183. 2 hits.
PfamiPF00664. ABC_membrane. 2 hits.
PF00005. ABC_tran. 2 hits.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF90123. SSF90123. 2 hits.
PROSITEiPS50929. ABC_TM1F. 2 hits.
PS00211. ABC_TRANSPORTER_1. 2 hits.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSTE6_YEAST
AccessioniPrimary (citable) accession number: P12866
Secondary accession number(s): D6VWZ4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 1, 1989
Last modified: November 2, 2016
This is version 167 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XI
    Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.