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P12866 (STE6_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 146. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Alpha-factor-transporting ATPase

EC=3.6.3.48
Alternative name(s):
Mating factor A secretion protein STE6
Multiple drug resistance protein homolog
P-glycoprotein
Gene names
Name:STE6
Ordered Locus Names:YKL209C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length1290 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

STE6 is required in yeast MATA cells for production of A-factor pheromone. STE6 is involved in the transport of the farnesyl-derivation of the A-factor pheromone.

Catalytic activity

ATP + H2O + alpha-factor(In) = ADP + phosphate + alpha-factor(Out).

Subcellular location

Membrane; Multi-pass membrane protein.

Post-translational modification

Degraded via the ubiquitin system.

Sequence similarities

Belongs to the ABC transporter superfamily. Alpha-factor sex pheromone exporter (TC 3.A.1.206) family. [View classification]

Contains 2 ABC transmembrane type-1 domains.

Contains 2 ABC transporter domains.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

SSM4P403182EBI-18383,EBI-18208

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 12901290Alpha-factor-transporting ATPase
PRO_0000093370

Regions

Topological domain1 – 2525Cytoplasmic Probable
Transmembrane26 – 4621Helical; Probable
Topological domain47 – 7529Extracellular Probable
Transmembrane76 – 9621Helical; Probable
Topological domain97 – 15054Cytoplasmic Probable
Transmembrane151 – 17121Helical; Probable
Topological domain172 – 1732Extracellular Probable
Transmembrane174 – 19421Helical; Probable
Topological domain195 – 26268Cytoplasmic Probable
Transmembrane263 – 28321Helical; Probable
Topological domain284 – 29613Extracellular Probable
Transmembrane297 – 31721Helical; Probable
Topological domain318 – 715398Cytoplasmic Probable
Transmembrane716 – 73621Helical; Probable
Topological domain737 – 76327Extracellular Probable
Transmembrane764 – 78421Helical; Probable
Topological domain785 – 83854Cytoplasmic Probable
Transmembrane839 – 85921Helical; Probable
Topological domain860 – 8656Extracellular Probable
Transmembrane866 – 88621Helical; Probable
Topological domain887 – 94559Cytoplasmic Probable
Transmembrane946 – 96621Helical; Probable
Topological domain967 – 98115Extracellular Probable
Transmembrane982 – 100221Helical; Probable
Topological domain1003 – 1290288Cytoplasmic Probable
Domain27 – 319293ABC transmembrane type-1 1
Domain357 – 603247ABC transporter 1
Domain717 – 1007291ABC transmembrane type-1 2
Domain1052 – 1287236ABC transporter 2
Nucleotide binding392 – 3998ATP 1 Potential
Nucleotide binding1087 – 10948ATP 2 Potential

Amino acid modifications

Glycosylation611N-linked (GlcNAc...) Potential
Cross-link1022Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Ref.9

Experimental info

Mutagenesis3921G → V: 0.8% mating activity.
Mutagenesis3981K → A: 25% mating activity.
Mutagenesis3981K → R: 1% mating activity.
Mutagenesis5091G → D: 0.5% mating activity.
Mutagenesis10871G → V: 0.3% mating activity.
Mutagenesis10931K → A: 26% mating activity.
Mutagenesis10931K → R: 15% mating activity.
Mutagenesis11931G → D: 6% mating activity.

Sequences

Sequence LengthMass (Da)Tools
P12866 [UniParc].

Last modified October 1, 1989. Version 1.
Checksum: B240B7E51D9680D5

FASTA1,290144,766
        10         20         30         40         50         60 
MNFLSFKTTK HYHIFRYVNI RNDYRLLMIM IIGTVATGLV PAITSILTGR VFDLLSVFVA 

        70         80         90        100        110        120 
NGSHQGLYSQ LVQRSMAVMA LGAASVPVMW LSLTSWMHIG ERQGFRIRSQ ILEAYLEEKP 

       130        140        150        160        170        180 
MEWYDNNEKL LGDFTQINRC VEELRSSSAE ASAITFQNLV AICALLGTSF YYSWSLTLII 

       190        200        210        220        230        240 
LCSSPIITFF AVVFSRMIHV YSEKENSETS KAAQLLTWSM NAAQLVRLYC TQRLERKKFK 

       250        260        270        280        290        300 
EIILNCNTFF IKSCFFVAAN AGILRFLTLT MFVQGFWFGS AMIKKGKLNI NDVITCFHSC 

       310        320        330        340        350        360 
IMLGSTLNNT LHQIVVLQKG GVAMEKIMTL LKDGSKRNPL NKTVAHQFPL DYATSDLTFA 

       370        380        390        400        410        420 
NVSFSYPSRP SEAVLKNVSL NFSAGQFTFI VGKSGSGKST LSNLLLRFYD GYNGSISING 

       430        440        450        460        470        480 
HNIQTIDQKL LIENITVVEQ RCTLFNDTLR KNILLGSTDS VRNADCSTNE NRHLIKDACQ 

       490        500        510        520        530        540 
MALLDRFILD LPDGLETLIG TGGVTLSGGQ QQRVAIARAF IRDTPILFLD EAVSALDIVH 

       550        560        570        580        590        600 
RNLLMKAIRH WRKGKTTIIL THELSQIESD DYLYLMKEGE VVESGTQSEL LADPTTTFST 

       610        620        630        640        650        660 
WYHLQNDYSD AKTIVDTETE EKSIHTVESF NSQLETPKLG SCLSNLGYDE TDQLSFYEAI 

       670        680        690        700        710        720 
YQKRSNVRTR RVKVEEENIG YALKQQKNTE SSTGPQLLSI IQIIKRMIKS IRYKKILILG 

       730        740        750        760        770        780 
LLCSLIAGAT NPVFSYTFSF LLEGIVPSTD GKTGSSHYLA KWSLLVLGVA AADGIFNFAK 

       790        800        810        820        830        840 
GFLLDCCSEY WVMDLRNEVM EKLTRKNMDW FSGENNKASE ISALVLNDLR DLRSLVSEFL 

       850        860        870        880        890        900 
SAMTSFVTVS TIGLIWALVS GWKLSLVCIS MFPLIIIFSA IYGGILQKCE TDYKTSVAQL 

       910        920        930        940        950        960 
ENCLYQIVTN IKTIKCLQAE FHFQLTYHDL KIKMQQIASK RAIATGFGIS MTNMIVMCIQ 

       970        980        990       1000       1010       1020 
AIIYYYGLKL VMIHEYTSKE MFTTFTLLLF TIMSCTSLVS QIPDISRGQR AASWIYRILD 

      1030       1040       1050       1060       1070       1080 
EKHNTLEVEN NNARTVGIAG HTYHGKEKKP IVSIQNLTFA YPSAPTAFVY KNMNFDMFCG 

      1090       1100       1110       1120       1130       1140 
QTLGIIGESG TGKSTLVLLL TKLYNCEVGK IKIDGTDVND WNLTSLRKEI SVVEQKPLLF 

      1150       1160       1170       1180       1190       1200 
NGTIRDNLTY GLQDEILEIE MYDALKYVGI HDFVISSPQG LDTRIDTTLL SGGQAQRLCI 

      1210       1220       1230       1240       1250       1260 
ARALLRKSKI LILDECTSAL DSVSSSIINE IVKKGPPALL TMVITHSEQM MRSCNSIAVL 

      1270       1280       1290 
KDGKVVERGN FDTLYNNRGE LFQIVSNQSS 

« Hide

References

« Hide 'large scale' references
[1]"The yeast STE6 gene encodes a homologue of the mammalian multidrug resistance P-glycoprotein."
McGrath J.P., Varshavsky A.
Nature 340:400-404(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"Saccharomyces cerevisiae STE6 gene product: a novel pathway for protein export in eukaryotic cells."
Kuchler K., Sterne R.E., Thorner J.W.
EMBO J. 8:3973-3984(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Complete DNA sequence of yeast chromosome XI."
Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. expand/collapse author list , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Sequences upstream of the STE6 gene required for its expression and regulation by the mating type locus in Saccharomyces cerevisiae."
Wilson K.L., Herskowitz I.
Proc. Natl. Acad. Sci. U.S.A. 83:2536-2540(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-41.
[6]"Mutational analysis of the yeast a-factor transporter STE6, a member of the ATP binding cassette (ABC) protein superfamily."
Berkover C., Michaelis S.
EMBO J. 10:3777-3785(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS.
[7]"The ABC-transporter Ste6 accumulates in the plasma membrane in a ubiquitinated form in endocytosis mutants."
Koelling R., Hollenberg C.
EMBO J. 13:3261-3271(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: DEGRADATION BY UBIQUITINATION.
[8]"Comparative topology studies in Saccharomyces cerevisiae and in Escherichia coli. The N-terminal half of the yeast ABC protein Ste6."
Geller D., Taglicht D., Edgar R., Tam A., Pines O., Michaelis S., Bibi E.
J. Biol. Chem. 271:13746-13753(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: TOPOLOGY.
[9]"A proteomics approach to understanding protein ubiquitination."
Peng J., Schwartz D., Elias J.E., Thoreen C.C., Cheng D., Marsischky G., Roelofs J., Finley D., Gygi S.P.
Nat. Biotechnol. 21:921-926(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-1022.
Strain: SUB592.
[10]"A global topology map of the Saccharomyces cerevisiae membrane proteome."
Kim H., Melen K., Oesterberg M., von Heijne G.
Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
Strain: ATCC 208353 / W303-1A.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X15428 Genomic DNA. Translation: CAA33467.1.
M26376 Genomic DNA. Translation: AAA35116.1.
Z28209 Genomic DNA. Translation: CAA82054.1.
M12842 Genomic DNA. Translation: AAA35117.1.
BK006944 Genomic DNA. Translation: DAA08960.1.
PIRDVBYS6. S05789.
RefSeqNP_012713.1. NM_001179774.1.

3D structure databases

ProteinModelPortalP12866.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33956. 24 interactions.
DIPDIP-2594N.
IntActP12866. 9 interactions.
MINTMINT-427396.
STRING4932.YKL209C.

Protein family/group databases

TCDB3.A.1.206.1. the atp-binding cassette (abc) superfamily.

Proteomic databases

MaxQBP12866.
PaxDbP12866.
PeptideAtlasP12866.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYKL209C; YKL209C; YKL209C.
GeneID853671.
KEGGsce:YKL209C.

Organism-specific databases

CYGDYKL209c.
SGDS000001692. STE6.

Phylogenomic databases

eggNOGCOG1132.
GeneTreeENSGT00530000062896.
HOGENOMHOG000093959.
KOK05658.
OMAMAWITHI.
OrthoDBEOG70KGXV.

Enzyme and pathway databases

BioCycYEAST:G3O-31968-MONOMER.
BRENDA3.6.3.48. 984.

Gene expression databases

GenevestigatorP12866.

Family and domain databases

Gene3D3.40.50.300. 2 hits.
InterProIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR001140. ABC_transptr_TM_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamPF00664. ABC_membrane. 2 hits.
PF00005. ABC_tran. 2 hits.
[Graphical view]
SMARTSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMSSF52540. SSF52540. 2 hits.
SSF90123. SSF90123. 2 hits.
PROSITEPS50929. ABC_TM1F. 2 hits.
PS00211. ABC_TRANSPORTER_1. 2 hits.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio974617.

Entry information

Entry nameSTE6_YEAST
AccessionPrimary (citable) accession number: P12866
Secondary accession number(s): D6VWZ4
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 1, 1989
Last modified: June 11, 2014
This is version 146 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XI

Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families