Alpha-factor-transporting ATPase - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P12866 (STE6_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 134. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Alpha-factor-transporting ATPase
EC=3.6.3.48

Alternative name(s):
Mating factor A secretion protein STE6
Multiple drug resistance protein homolog
P-glycoprotein

Gene names

Name:	STE6
Ordered Locus Names:	YKL209C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	1290 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	STE6 is required in yeast MATA cells for production of A-factor pheromone. STE6 is involved in the transport of the farnesyl-derivation of the A-factor pheromone.
Catalytic activity	ATP + H₂O + alpha-factor(In) = ADP + phosphate + alpha-factor(Out).
Subcellular location	Membrane; Multi-pass membrane protein.
Post-translational modification	Degraded via the ubiquitin system.
Sequence similarities	Belongs to the ABC transporter superfamily. Alpha-factor sex pheromone exporter (TC 3.A.1.206) family. [View classification] Contains 2 ABC transmembrane type-1 domains. Contains 2 ABC transporter domains.

Ontologies

Keywords
Biological process	Pheromone response Transport
Cellular component	Membrane
Domain	Repeat Transmembrane Transmembrane helix
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase
PTM	Glycoprotein Isopeptide bond Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	peptide pheromone export Inferred from mutant phenotype Ref.1. Source: SGD response to pheromone Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	Golgi apparatus Inferred from direct assay Ref.7. Source: SGD integral to membrane Inferred from sequence model PubMed 12192589. Source: SGD mating projection tip Inferred from direct assay PubMed 12374868 PubMed 7679674. Source: SGD plasma membrane Inferred from direct assay PubMed 7679674. Source: SGD
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW peptide-transporting ATPase activity Inferred from direct assay PubMed 11389139 PubMed 8626301. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
SSM4	P40318	2	EBI-18383,EBI-18208

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1290

1290

Alpha-factor-transporting ATPase

PRO_0000093370

Regions

Topological domain

1 – 25

Cytoplasmic Probable

Transmembrane

26 – 46

Helical; Probable

Topological domain

47 – 75

Extracellular Probable

Transmembrane

76 – 96

Helical; Probable

Topological domain

97 – 150

Cytoplasmic Probable

Transmembrane

151 – 171

Helical; Probable

Topological domain

172 – 173

Extracellular Probable

Transmembrane

174 – 194

Helical; Probable

Topological domain

195 – 262

Cytoplasmic Probable

Transmembrane

263 – 283

Helical; Probable

Topological domain

284 – 296

Extracellular Probable

Transmembrane

297 – 317

Helical; Probable

Topological domain

318 – 715

398

Cytoplasmic Probable

Transmembrane

716 – 736

Helical; Probable

Topological domain

737 – 763

Extracellular Probable

Transmembrane

764 – 784

Helical; Probable

Topological domain

785 – 838

Cytoplasmic Probable

Transmembrane

839 – 859

Helical; Probable

Topological domain

860 – 865

Extracellular Probable

Transmembrane

866 – 886

Helical; Probable

Topological domain

887 – 945

Cytoplasmic Probable

Transmembrane

946 – 966

Helical; Probable

Topological domain

967 – 981

Extracellular Probable

Transmembrane

982 – 1002

Helical; Probable

Topological domain

1003 – 1290

288

Cytoplasmic Probable

Domain

27 – 319

293

ABC transmembrane type-1 1

Domain

357 – 603

247

ABC transporter 1

Domain

717 – 1007

291

ABC transmembrane type-1 2

Domain

1052 – 1287

236

ABC transporter 2

Nucleotide binding

392 – 399

ATP 1 Potential

Nucleotide binding

1087 – 1094

ATP 2 Potential

Amino acid modifications

Glycosylation

N-linked (GlcNAc...) Potential

Cross-link

1022

Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Ref.9

Experimental info

Mutagenesis

392

G → V: 0.8% mating activity.

Mutagenesis

398

K → A: 25% mating activity.

Mutagenesis

398

K → R: 1% mating activity.

Mutagenesis

509

G → D: 0.5% mating activity.

Mutagenesis

1087

G → V: 0.3% mating activity.

Mutagenesis

1093

K → A: 26% mating activity.

Mutagenesis

1093

K → R: 15% mating activity.

Mutagenesis

1193

G → D: 6% mating activity.

Sequences

Sequence

Length

Mass (Da)

Tools

P12866 [UniParc].

Last modified October 1, 1989. Version 1.
Checksum: B240B7E51D9680D5
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FASTA

1,290

144,766

        10         20         30         40         50         60 
MNFLSFKTTK HYHIFRYVNI RNDYRLLMIM IIGTVATGLV PAITSILTGR VFDLLSVFVA 

        70         80         90        100        110        120 
NGSHQGLYSQ LVQRSMAVMA LGAASVPVMW LSLTSWMHIG ERQGFRIRSQ ILEAYLEEKP 

       130        140        150        160        170        180 
MEWYDNNEKL LGDFTQINRC VEELRSSSAE ASAITFQNLV AICALLGTSF YYSWSLTLII 

       190        200        210        220        230        240 
LCSSPIITFF AVVFSRMIHV YSEKENSETS KAAQLLTWSM NAAQLVRLYC TQRLERKKFK 

       250        260        270        280        290        300 
EIILNCNTFF IKSCFFVAAN AGILRFLTLT MFVQGFWFGS AMIKKGKLNI NDVITCFHSC 

       310        320        330        340        350        360 
IMLGSTLNNT LHQIVVLQKG GVAMEKIMTL LKDGSKRNPL NKTVAHQFPL DYATSDLTFA 

       370        380        390        400        410        420 
NVSFSYPSRP SEAVLKNVSL NFSAGQFTFI VGKSGSGKST LSNLLLRFYD GYNGSISING 

       430        440        450        460        470        480 
HNIQTIDQKL LIENITVVEQ RCTLFNDTLR KNILLGSTDS VRNADCSTNE NRHLIKDACQ 

       490        500        510        520        530        540 
MALLDRFILD LPDGLETLIG TGGVTLSGGQ QQRVAIARAF IRDTPILFLD EAVSALDIVH 

       550        560        570        580        590        600 
RNLLMKAIRH WRKGKTTIIL THELSQIESD DYLYLMKEGE VVESGTQSEL LADPTTTFST 

       610        620        630        640        650        660 
WYHLQNDYSD AKTIVDTETE EKSIHTVESF NSQLETPKLG SCLSNLGYDE TDQLSFYEAI 

       670        680        690        700        710        720 
YQKRSNVRTR RVKVEEENIG YALKQQKNTE SSTGPQLLSI IQIIKRMIKS IRYKKILILG 

       730        740        750        760        770        780 
LLCSLIAGAT NPVFSYTFSF LLEGIVPSTD GKTGSSHYLA KWSLLVLGVA AADGIFNFAK 

       790        800        810        820        830        840 
GFLLDCCSEY WVMDLRNEVM EKLTRKNMDW FSGENNKASE ISALVLNDLR DLRSLVSEFL 

       850        860        870        880        890        900 
SAMTSFVTVS TIGLIWALVS GWKLSLVCIS MFPLIIIFSA IYGGILQKCE TDYKTSVAQL 

       910        920        930        940        950        960 
ENCLYQIVTN IKTIKCLQAE FHFQLTYHDL KIKMQQIASK RAIATGFGIS MTNMIVMCIQ 

       970        980        990       1000       1010       1020 
AIIYYYGLKL VMIHEYTSKE MFTTFTLLLF TIMSCTSLVS QIPDISRGQR AASWIYRILD 

      1030       1040       1050       1060       1070       1080 
EKHNTLEVEN NNARTVGIAG HTYHGKEKKP IVSIQNLTFA YPSAPTAFVY KNMNFDMFCG 

      1090       1100       1110       1120       1130       1140 
QTLGIIGESG TGKSTLVLLL TKLYNCEVGK IKIDGTDVND WNLTSLRKEI SVVEQKPLLF 

      1150       1160       1170       1180       1190       1200 
NGTIRDNLTY GLQDEILEIE MYDALKYVGI HDFVISSPQG LDTRIDTTLL SGGQAQRLCI 

      1210       1220       1230       1240       1250       1260 
ARALLRKSKI LILDECTSAL DSVSSSIINE IVKKGPPALL TMVITHSEQM MRSCNSIAVL 

      1270       1280       1290 
KDGKVVERGN FDTLYNNRGE LFQIVSNQSS

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The yeast STE6 gene encodes a homologue of the mammalian multidrug resistance P-glycoprotein." McGrath J.P., Varshavsky A. Nature 340:400-404(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Saccharomyces cerevisiae STE6 gene product: a novel pathway for protein export in eukaryotic cells." Kuchler K., Sterne R.E., Thorner J.W. EMBO J. 8:3973-3984(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Complete DNA sequence of yeast chromosome XI." Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C. Mewes H.-W. Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Sequences upstream of the STE6 gene required for its expression and regulation by the mating type locus in Saccharomyces cerevisiae." Wilson K.L., Herskowitz I. Proc. Natl. Acad. Sci. U.S.A. 83:2536-2540(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-41.
[6]	"Mutational analysis of the yeast a-factor transporter STE6, a member of the ATP binding cassette (ABC) protein superfamily." Berkover C., Michaelis S. EMBO J. 10:3777-3785(1991) [PubMed] [Europe PMC] [Abstract] Cited for: MUTAGENESIS.
[7]	"The ABC-transporter Ste6 accumulates in the plasma membrane in a ubiquitinated form in endocytosis mutants." Koelling R., Hollenberg C. EMBO J. 13:3261-3271(1994) [PubMed] [Europe PMC] [Abstract] Cited for: DEGRADATION BY UBIQUITINATION.
[8]	"Comparative topology studies in Saccharomyces cerevisiae and in Escherichia coli. The N-terminal half of the yeast ABC protein Ste6." Geller D., Taglicht D., Edgar R., Tam A., Pines O., Michaelis S., Bibi E. J. Biol. Chem. 271:13746-13753(1996) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY.
[9]	"A proteomics approach to understanding protein ubiquitination." Peng J., Schwartz D., Elias J.E., Thoreen C.C., Cheng D., Marsischky G., Roelofs J., Finley D., Gygi S.P. Nat. Biotechnol. 21:921-926(2003) [PubMed] [Europe PMC] [Abstract] Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-1022, MASS SPECTROMETRY. Strain: SUB592.
[10]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: ATCC 208353 / W303-1A.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X15428 Genomic DNA. Translation: CAA33467.1. M26376 Genomic DNA. Translation: AAA35116.1. Z28209 Genomic DNA. Translation: CAA82054.1. M12842 Genomic DNA. Translation: AAA35117.1. BK006944 Genomic DNA. Translation: DAA08960.1.
PIR	DVBYS6. S05789.
RefSeq	NP_012713.1. NM_001179774.1.
3D structure databases
ProteinModelPortal	P12866.
SMR	P12866. Positions 24-637, 714-1289.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2594N.
IntAct	P12866. 7 interactions.
MINT	MINT-427396.
STRING	4932.YKL209C.
Protein family/group databases
TCDB	3.A.1.206.1. ATP-binding cassette (ABC) superfamily.
Proteomic databases
PaxDb	P12866.
PeptideAtlas	P12866.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YKL209C; YKL209C; YKL209C.
GeneID	853671.
KEGG	sce:YKL209C.
Organism-specific databases
CYGD	YKL209c.
SGD	S000001692. STE6.
Phylogenomic databases
eggNOG	COG1132.
GeneTree	ENSGT00530000062896.
HOGENOM	HOG000093959.
KO	K05658.
OMA	VENNNAR.
OrthoDB	EOG469V2X.
Enzyme and pathway databases
BRENDA	3.6.3.48. 984.
Gene expression databases
Genevestigator	P12866.
GermOnline	YKL209C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR003593. AAA+_ATPase. IPR003439. ABC_transporter-like. IPR017871. ABC_transporter_CS. IPR017940. ABC_transporter_type1. IPR001140. ABC_transptr_TM_dom. IPR011527. ABC_transptrTM_dom_typ1. [Graphical view]
Pfam	PF00664. ABC_membrane. 2 hits. PF00005. ABC_tran. 2 hits. [Graphical view]
SMART	SM00382. AAA. 2 hits. [Graphical view]
SUPFAM	SSF90123. ABC_TM_1. 2 hits.
PROSITE	PS50929. ABC_TM1F. 2 hits. PS00211. ABC_TRANSPORTER_1. 2 hits. PS50893. ABC_TRANSPORTER_2. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	974617.

Entry information

Entry name

STE6_YEAST

Accession

Primary (citable) accession number: P12866
Secondary accession number(s): D6VWZ4

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	October 1, 1989
Last modified:	May 1, 2013
This is version 134 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome XI

Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents