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P12866

- STE6_YEAST

UniProt

P12866 - STE6_YEAST

Protein

Alpha-factor-transporting ATPase

Gene

STE6

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 147 (01 Oct 2014)
      Sequence version 1 (01 Oct 1989)
      Previous versions | rss
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    Functioni

    STE6 is required in yeast MATA cells for production of A-factor pheromone. STE6 is involved in the transport of the farnesyl-derivation of the A-factor pheromone.

    Catalytic activityi

    ATP + H2O + alpha-factor(In) = ADP + phosphate + alpha-factor(Out).

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi392 – 3998ATP 1PROSITE-ProRule annotation
    Nucleotide bindingi1087 – 10948ATP 2PROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. peptide-transporting ATPase activity Source: SGD
    3. protein binding Source: IntAct

    GO - Biological processi

    1. peptide pheromone export Source: SGD
    2. response to pheromone Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Pheromone response, Transport

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31968-MONOMER.
    BRENDAi3.6.3.48. 984.

    Protein family/group databases

    TCDBi3.A.1.206.1. the atp-binding cassette (abc) superfamily.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alpha-factor-transporting ATPase (EC:3.6.3.48)
    Alternative name(s):
    Mating factor A secretion protein STE6
    Multiple drug resistance protein homolog
    P-glycoprotein
    Gene namesi
    Name:STE6
    Ordered Locus Names:YKL209C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XI

    Organism-specific databases

    CYGDiYKL209c.
    SGDiS000001692. STE6.

    Subcellular locationi

    GO - Cellular componenti

    1. Golgi apparatus Source: SGD
    2. integral component of membrane Source: UniProtKB-KW
    3. mating projection tip Source: SGD
    4. plasma membrane Source: SGD

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi392 – 3921G → V: 0.8% mating activity. 1 Publication
    Mutagenesisi398 – 3981K → A: 25% mating activity. 1 Publication
    Mutagenesisi398 – 3981K → R: 1% mating activity. 1 Publication
    Mutagenesisi509 – 5091G → D: 0.5% mating activity. 1 Publication
    Mutagenesisi1087 – 10871G → V: 0.3% mating activity. 1 Publication
    Mutagenesisi1093 – 10931K → A: 26% mating activity. 1 Publication
    Mutagenesisi1093 – 10931K → R: 15% mating activity. 1 Publication
    Mutagenesisi1193 – 11931G → D: 6% mating activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 12901290Alpha-factor-transporting ATPasePRO_0000093370Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi61 – 611N-linked (GlcNAc...)Sequence Analysis
    Cross-linki1022 – 1022Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication

    Post-translational modificationi

    Degraded via the ubiquitin system.1 Publication

    Keywords - PTMi

    Glycoprotein, Isopeptide bond, Ubl conjugation

    Proteomic databases

    MaxQBiP12866.
    PaxDbiP12866.
    PeptideAtlasiP12866.

    Expressioni

    Gene expression databases

    GenevestigatoriP12866.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    SSM4P403182EBI-18383,EBI-18208

    Protein-protein interaction databases

    BioGridi33956. 24 interactions.
    DIPiDIP-2594N.
    IntActiP12866. 9 interactions.
    MINTiMINT-427396.
    STRINGi4932.YKL209C.

    Structurei

    3D structure databases

    ProteinModelPortaliP12866.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 2525CytoplasmicCuratedAdd
    BLAST
    Topological domaini47 – 7529ExtracellularCuratedAdd
    BLAST
    Topological domaini97 – 15054CytoplasmicCuratedAdd
    BLAST
    Topological domaini172 – 1732ExtracellularCurated
    Topological domaini195 – 26268CytoplasmicCuratedAdd
    BLAST
    Topological domaini284 – 29613ExtracellularCuratedAdd
    BLAST
    Topological domaini318 – 715398CytoplasmicCuratedAdd
    BLAST
    Topological domaini737 – 76327ExtracellularCuratedAdd
    BLAST
    Topological domaini785 – 83854CytoplasmicCuratedAdd
    BLAST
    Topological domaini860 – 8656ExtracellularCurated
    Topological domaini887 – 94559CytoplasmicCuratedAdd
    BLAST
    Topological domaini967 – 98115ExtracellularCuratedAdd
    BLAST
    Topological domaini1003 – 1290288CytoplasmicCuratedAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei26 – 4621HelicalCuratedAdd
    BLAST
    Transmembranei76 – 9621HelicalCuratedAdd
    BLAST
    Transmembranei151 – 17121HelicalCuratedAdd
    BLAST
    Transmembranei174 – 19421HelicalCuratedAdd
    BLAST
    Transmembranei263 – 28321HelicalCuratedAdd
    BLAST
    Transmembranei297 – 31721HelicalCuratedAdd
    BLAST
    Transmembranei716 – 73621HelicalCuratedAdd
    BLAST
    Transmembranei764 – 78421HelicalCuratedAdd
    BLAST
    Transmembranei839 – 85921HelicalCuratedAdd
    BLAST
    Transmembranei866 – 88621HelicalCuratedAdd
    BLAST
    Transmembranei946 – 96621HelicalCuratedAdd
    BLAST
    Transmembranei982 – 100221HelicalCuratedAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini27 – 319293ABC transmembrane type-1 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini357 – 603247ABC transporter 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini717 – 1007291ABC transmembrane type-1 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini1052 – 1287236ABC transporter 2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 2 ABC transmembrane type-1 domains.PROSITE-ProRule annotation
    Contains 2 ABC transporter domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1132.
    GeneTreeiENSGT00530000062896.
    HOGENOMiHOG000093959.
    KOiK05658.
    OMAiMAWITHI.
    OrthoDBiEOG70KGXV.

    Family and domain databases

    Gene3Di3.40.50.300. 2 hits.
    InterProiIPR003593. AAA+_ATPase.
    IPR011527. ABC1_TM_dom.
    IPR003439. ABC_transporter-like.
    IPR017871. ABC_transporter_CS.
    IPR001140. ABC_transptr_TM_dom.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PfamiPF00664. ABC_membrane. 2 hits.
    PF00005. ABC_tran. 2 hits.
    [Graphical view]
    SMARTiSM00382. AAA. 2 hits.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 2 hits.
    SSF90123. SSF90123. 2 hits.
    PROSITEiPS50929. ABC_TM1F. 2 hits.
    PS00211. ABC_TRANSPORTER_1. 2 hits.
    PS50893. ABC_TRANSPORTER_2. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P12866-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNFLSFKTTK HYHIFRYVNI RNDYRLLMIM IIGTVATGLV PAITSILTGR     50
    VFDLLSVFVA NGSHQGLYSQ LVQRSMAVMA LGAASVPVMW LSLTSWMHIG 100
    ERQGFRIRSQ ILEAYLEEKP MEWYDNNEKL LGDFTQINRC VEELRSSSAE 150
    ASAITFQNLV AICALLGTSF YYSWSLTLII LCSSPIITFF AVVFSRMIHV 200
    YSEKENSETS KAAQLLTWSM NAAQLVRLYC TQRLERKKFK EIILNCNTFF 250
    IKSCFFVAAN AGILRFLTLT MFVQGFWFGS AMIKKGKLNI NDVITCFHSC 300
    IMLGSTLNNT LHQIVVLQKG GVAMEKIMTL LKDGSKRNPL NKTVAHQFPL 350
    DYATSDLTFA NVSFSYPSRP SEAVLKNVSL NFSAGQFTFI VGKSGSGKST 400
    LSNLLLRFYD GYNGSISING HNIQTIDQKL LIENITVVEQ RCTLFNDTLR 450
    KNILLGSTDS VRNADCSTNE NRHLIKDACQ MALLDRFILD LPDGLETLIG 500
    TGGVTLSGGQ QQRVAIARAF IRDTPILFLD EAVSALDIVH RNLLMKAIRH 550
    WRKGKTTIIL THELSQIESD DYLYLMKEGE VVESGTQSEL LADPTTTFST 600
    WYHLQNDYSD AKTIVDTETE EKSIHTVESF NSQLETPKLG SCLSNLGYDE 650
    TDQLSFYEAI YQKRSNVRTR RVKVEEENIG YALKQQKNTE SSTGPQLLSI 700
    IQIIKRMIKS IRYKKILILG LLCSLIAGAT NPVFSYTFSF LLEGIVPSTD 750
    GKTGSSHYLA KWSLLVLGVA AADGIFNFAK GFLLDCCSEY WVMDLRNEVM 800
    EKLTRKNMDW FSGENNKASE ISALVLNDLR DLRSLVSEFL SAMTSFVTVS 850
    TIGLIWALVS GWKLSLVCIS MFPLIIIFSA IYGGILQKCE TDYKTSVAQL 900
    ENCLYQIVTN IKTIKCLQAE FHFQLTYHDL KIKMQQIASK RAIATGFGIS 950
    MTNMIVMCIQ AIIYYYGLKL VMIHEYTSKE MFTTFTLLLF TIMSCTSLVS 1000
    QIPDISRGQR AASWIYRILD EKHNTLEVEN NNARTVGIAG HTYHGKEKKP 1050
    IVSIQNLTFA YPSAPTAFVY KNMNFDMFCG QTLGIIGESG TGKSTLVLLL 1100
    TKLYNCEVGK IKIDGTDVND WNLTSLRKEI SVVEQKPLLF NGTIRDNLTY 1150
    GLQDEILEIE MYDALKYVGI HDFVISSPQG LDTRIDTTLL SGGQAQRLCI 1200
    ARALLRKSKI LILDECTSAL DSVSSSIINE IVKKGPPALL TMVITHSEQM 1250
    MRSCNSIAVL KDGKVVERGN FDTLYNNRGE LFQIVSNQSS 1290
    Length:1,290
    Mass (Da):144,766
    Last modified:October 1, 1989 - v1
    Checksum:iB240B7E51D9680D5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X15428 Genomic DNA. Translation: CAA33467.1.
    M26376 Genomic DNA. Translation: AAA35116.1.
    Z28209 Genomic DNA. Translation: CAA82054.1.
    M12842 Genomic DNA. Translation: AAA35117.1.
    BK006944 Genomic DNA. Translation: DAA08960.1.
    PIRiS05789. DVBYS6.
    RefSeqiNP_012713.1. NM_001179774.1.

    Genome annotation databases

    EnsemblFungiiYKL209C; YKL209C; YKL209C.
    GeneIDi853671.
    KEGGisce:YKL209C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X15428 Genomic DNA. Translation: CAA33467.1 .
    M26376 Genomic DNA. Translation: AAA35116.1 .
    Z28209 Genomic DNA. Translation: CAA82054.1 .
    M12842 Genomic DNA. Translation: AAA35117.1 .
    BK006944 Genomic DNA. Translation: DAA08960.1 .
    PIRi S05789. DVBYS6.
    RefSeqi NP_012713.1. NM_001179774.1.

    3D structure databases

    ProteinModelPortali P12866.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33956. 24 interactions.
    DIPi DIP-2594N.
    IntActi P12866. 9 interactions.
    MINTi MINT-427396.
    STRINGi 4932.YKL209C.

    Protein family/group databases

    TCDBi 3.A.1.206.1. the atp-binding cassette (abc) superfamily.

    Proteomic databases

    MaxQBi P12866.
    PaxDbi P12866.
    PeptideAtlasi P12866.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YKL209C ; YKL209C ; YKL209C .
    GeneIDi 853671.
    KEGGi sce:YKL209C.

    Organism-specific databases

    CYGDi YKL209c.
    SGDi S000001692. STE6.

    Phylogenomic databases

    eggNOGi COG1132.
    GeneTreei ENSGT00530000062896.
    HOGENOMi HOG000093959.
    KOi K05658.
    OMAi MAWITHI.
    OrthoDBi EOG70KGXV.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31968-MONOMER.
    BRENDAi 3.6.3.48. 984.

    Miscellaneous databases

    NextBioi 974617.

    Gene expression databases

    Genevestigatori P12866.

    Family and domain databases

    Gene3Di 3.40.50.300. 2 hits.
    InterProi IPR003593. AAA+_ATPase.
    IPR011527. ABC1_TM_dom.
    IPR003439. ABC_transporter-like.
    IPR017871. ABC_transporter_CS.
    IPR001140. ABC_transptr_TM_dom.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    Pfami PF00664. ABC_membrane. 2 hits.
    PF00005. ABC_tran. 2 hits.
    [Graphical view ]
    SMARTi SM00382. AAA. 2 hits.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 2 hits.
    SSF90123. SSF90123. 2 hits.
    PROSITEi PS50929. ABC_TM1F. 2 hits.
    PS00211. ABC_TRANSPORTER_1. 2 hits.
    PS50893. ABC_TRANSPORTER_2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The yeast STE6 gene encodes a homologue of the mammalian multidrug resistance P-glycoprotein."
      McGrath J.P., Varshavsky A.
      Nature 340:400-404(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. "Saccharomyces cerevisiae STE6 gene product: a novel pathway for protein export in eukaryotic cells."
      Kuchler K., Sterne R.E., Thorner J.W.
      EMBO J. 8:3973-3984(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Complete DNA sequence of yeast chromosome XI."
      Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V., Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P., Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L., Daignan-Fornier B., del Rey F., Dion C.
      , Domdey H., Duesterhoeft A., Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H., Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L., Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M., Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H., Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J., Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H., Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J., Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S., Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F., Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R., Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W., Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M., Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C., Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H., Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L., van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S., von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M., Becker I., Mewes H.-W.
      Nature 369:371-378(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "Sequences upstream of the STE6 gene required for its expression and regulation by the mating type locus in Saccharomyces cerevisiae."
      Wilson K.L., Herskowitz I.
      Proc. Natl. Acad. Sci. U.S.A. 83:2536-2540(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-41.
    6. "Mutational analysis of the yeast a-factor transporter STE6, a member of the ATP binding cassette (ABC) protein superfamily."
      Berkover C., Michaelis S.
      EMBO J. 10:3777-3785(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS.
    7. "The ABC-transporter Ste6 accumulates in the plasma membrane in a ubiquitinated form in endocytosis mutants."
      Koelling R., Hollenberg C.
      EMBO J. 13:3261-3271(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: DEGRADATION BY UBIQUITINATION.
    8. "Comparative topology studies in Saccharomyces cerevisiae and in Escherichia coli. The N-terminal half of the yeast ABC protein Ste6."
      Geller D., Taglicht D., Edgar R., Tam A., Pines O., Michaelis S., Bibi E.
      J. Biol. Chem. 271:13746-13753(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: TOPOLOGY.
    9. Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-1022.
      Strain: SUB592.
    10. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
      Kim H., Melen K., Oesterberg M., von Heijne G.
      Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
      Strain: ATCC 208353 / W303-1A.

    Entry informationi

    Entry nameiSTE6_YEAST
    AccessioniPrimary (citable) accession number: P12866
    Secondary accession number(s): D6VWZ4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1989
    Last sequence update: October 1, 1989
    Last modified: October 1, 2014
    This is version 147 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XI
      Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names

    External Data

    Dasty 3