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Protein

Glyceraldehyde-3-phosphate dehydrogenase B, chloroplastic

Gene

GAPB

Organism
Pisum sativum (Garden pea)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

D-glyceraldehyde 3-phosphate + phosphate + NADP+ = 3-phospho-D-glyceroyl phosphate + NADPH.

Pathwayi: Calvin cycle

This protein is involved in the pathway Calvin cycle, which is part of Carbohydrate biosynthesis.
View all proteins of this organism that are known to be involved in the pathway Calvin cycle and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei119 – 1191NADPBy similarity
Binding sitei164 – 1641NADP; via carbonyl oxygenBy similarity
Active sitei239 – 2391NucleophilePROSITE-ProRule annotation
Sitei266 – 2661Activates thiol group during catalysisBy similarity
Binding sitei269 – 2691Glyceraldehyde 3-phosphateBy similarity
Binding sitei284 – 2841Glyceraldehyde 3-phosphateBy similarity
Binding sitei320 – 3201Glyceraldehyde 3-phosphateBy similarity
Binding sitei403 – 4031NADPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi95 – 962NADPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Calvin cycle

Keywords - Ligandi

NADP

Enzyme and pathway databases

UniPathwayiUPA00116.

Names & Taxonomyi

Protein namesi
Recommended name:
Glyceraldehyde-3-phosphate dehydrogenase B, chloroplastic (EC:1.2.1.13)
Alternative name(s):
NADP-dependent glyceraldehydephosphate dehydrogenase subunit B
Gene namesi
Name:GAPB
Synonyms:GPB1
OrganismiPisum sativum (Garden pea)
Taxonomic identifieri3888 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeFabeaePisum

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 8484ChloroplastAdd
BLAST
Chaini85 – 451367Glyceraldehyde-3-phosphate dehydrogenase B, chloroplasticPRO_0000010424Add
BLAST

Proteomic databases

PRIDEiP12859.

Interactioni

Subunit structurei

Tetramer of either four A chains (GAPDH 2) or two A and two B chains (GAPDH 1).By similarity

Protein-protein interaction databases

DIPiDIP-29837N.

Structurei

3D structure databases

ProteinModelPortaliP12859.
SMRiP12859. Positions 85-424.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni238 – 2403Glyceraldehyde 3-phosphate bindingBy similarity
Regioni297 – 2982Glyceraldehyde 3-phosphate bindingBy similarity

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR003823. DUF_CP12.
IPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006424. Glyceraldehyde-3-P_DH_1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR10836. PTHR10836. 1 hit.
PfamiPF02672. CP12. 1 hit.
PF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
[Graphical view]
PRINTSiPR00078. G3PDHDRGNASE.
SMARTiSM00846. Gp_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01534. GAPDH-I. 1 hit.
PROSITEiPS00071. GAPDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P12859-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATHAALAST RIPTNTRFPS KTSHSFPSQC ASKRLEVGEF SGLKSTSCIS
60 70 80 90 100
YVHSARDSSF YDVVAAQLTS KANGSTAVKG VTVAKLKVAI NGFGRIGRNF
110 120 130 140 150
LRCWHGRKDS PLEVIVVNDS GGVKNASHLL KYDSMLGTFK AEVKILNNET
160 170 180 190 200
ITVDGKPIKV VSSRDPLKLP WAELGIDIVI EGTGVFVDGP GAGKHIQAGA
210 220 230 240 250
KKVIITAPAK GADIPTYVIG VNEQDYGHEV ADIISNASCT TNCLAPFAKV
260 270 280 290 300
LDEEFGIVKG TMTTTHSYTG DQRLLDASHR DLRRARAAAL NIVPTSTGAA
310 320 330 340 350
KAVSLVLPQL KGKLNGIALR VPTPNVSVVD LVVNVAKKGI SAEDVNAAFR
360 370 380 390 400
KAAEGPLKGI LDVCDVPLVS VDFRCSDVST TIDSSLTMVM GDDMVKVVAW
410 420 430 440 450
YDNEWGYSQR VVDLAHLVAN KWPGTPKVGS GDPLEDFCET NPADEECKVY

E
Length:451
Mass (Da):48,097
Last modified:May 1, 1992 - v2
Checksum:iC5D63CE37B19CE57
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti255 – 2551F → S in CAA33262 (PubMed:2562762).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M55147 Genomic DNA. Translation: AAA84543.1.
X15188 mRNA. Translation: CAA33262.1.
PIRiS16507. DEPMNB.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M55147 Genomic DNA. Translation: AAA84543.1.
X15188 mRNA. Translation: CAA33262.1.
PIRiS16507. DEPMNB.

3D structure databases

ProteinModelPortaliP12859.
SMRiP12859. Positions 85-424.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-29837N.

Proteomic databases

PRIDEiP12859.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00116.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR003823. DUF_CP12.
IPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006424. Glyceraldehyde-3-P_DH_1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR10836. PTHR10836. 1 hit.
PfamiPF02672. CP12. 1 hit.
PF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
[Graphical view]
PRINTSiPR00078. G3PDHDRGNASE.
SMARTiSM00846. Gp_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01534. GAPDH-I. 1 hit.
PROSITEiPS00071. GAPDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Differential intron loss and endosymbiotic transfer of chloroplast glyceraldehyde-3-phosphate dehydrogenase genes to the nucleus."
    Liaud M.-F., Zhang D.-X., Cerff R.
    Proc. Natl. Acad. Sci. U.S.A. 87:8918-8922(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: cv. Rosakrone.
    Tissue: Seedling.
  2. "Cloning and sequence analysis of cDNAs encoding the cytosolic precursors of subunits GapA and GapB of chloroplast glyceraldehyde-3-phosphate dehydrogenase from pea and spinach."
    Brinkmann H., Cerff R., Salomon M., Soll J.
    Plant Mol. Biol. 13:81-94(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 5-451.
    Strain: cv. Rosakrone.
    Tissue: Seedling.

Entry informationi

Entry nameiG3PB_PEA
AccessioniPrimary (citable) accession number: P12859
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: May 1, 1992
Last modified: December 9, 2015
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Plants contain two types of GAPDH: cytosolic forms which participate in glycolysis and chloroplast forms which participate in photosynthesis. All the forms are encoded by distinct genes.

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.