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P12859 (G3PB_PEA) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Glyceraldehyde-3-phosphate dehydrogenase B, chloroplastic
EC=1.2.1.13
Alternative name(s):
NADP-dependent glyceraldehydephosphate dehydrogenase subunit B
Gene names
Name:GAPB
Synonyms:GPB1
OrganismPisum sativum (Garden pea)
Taxonomic identifier3888 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Fabeae › Pisum

Protein attributes

Sequence length451 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

D-glyceraldehyde 3-phosphate + phosphate + NADP+ = 3-phospho-D-glyceroyl phosphate + NADPH.

Pathway

Carbohydrate biosynthesis; Calvin cycle.

Subunit structure

Tetramer of either four A chains (GAPDH 2) or two A and two B chains (GAPDH 1).

Subcellular location

Plastid › chloroplast.

Miscellaneous

Plants contain three forms of GAPDH: a cytosolic form which participates in glycolysis and two chloroplast forms which participates in photosynthesis. These three forms are encoded by distinct genes.

Sequence similarities

Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

Ontologies

Keywords
   Biological processCalvin cycle
   Cellular componentChloroplast
Plastid
   DomainTransit peptide
   LigandNADP
   Molecular functionOxidoreductase
Gene Ontology (GO)
   Biological processglucose metabolic process

Inferred from electronic annotation. Source: InterPro

reductive pentose-phosphate cycle

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentchloroplast

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionNAD binding

Inferred from electronic annotation. Source: InterPro

NADP binding

Inferred from electronic annotation. Source: InterPro

glyceraldehyde-3-phosphate dehydrogenase (NADP+) (phosphorylating) activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 8484Chloroplast
Chain85 – 451367Glyceraldehyde-3-phosphate dehydrogenase B, chloroplastic
PRO_0000010424

Regions

Nucleotide binding95 – 962NADP By similarity
Region238 – 2403Glyceraldehyde 3-phosphate binding By similarity
Region297 – 2982Glyceraldehyde 3-phosphate binding By similarity

Sites

Active site2391Nucleophile By similarity
Binding site1191NADP By similarity
Binding site1641NADP; via carbonyl oxygen By similarity
Binding site2691Glyceraldehyde 3-phosphate By similarity
Binding site2841Glyceraldehyde 3-phosphate By similarity
Binding site3201Glyceraldehyde 3-phosphate By similarity
Binding site4031NADP By similarity
Site2661Activates thiol group during catalysis By similarity

Experimental info

Sequence conflict2551F → S in CAA33262. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P12859 [UniParc].

Last modified May 1, 1992. Version 2.
Checksum: C5D63CE37B19CE57

FASTA45148,097
        10         20         30         40         50         60 
MATHAALAST RIPTNTRFPS KTSHSFPSQC ASKRLEVGEF SGLKSTSCIS YVHSARDSSF 

        70         80         90        100        110        120 
YDVVAAQLTS KANGSTAVKG VTVAKLKVAI NGFGRIGRNF LRCWHGRKDS PLEVIVVNDS 

       130        140        150        160        170        180 
GGVKNASHLL KYDSMLGTFK AEVKILNNET ITVDGKPIKV VSSRDPLKLP WAELGIDIVI 

       190        200        210        220        230        240 
EGTGVFVDGP GAGKHIQAGA KKVIITAPAK GADIPTYVIG VNEQDYGHEV ADIISNASCT 

       250        260        270        280        290        300 
TNCLAPFAKV LDEEFGIVKG TMTTTHSYTG DQRLLDASHR DLRRARAAAL NIVPTSTGAA 

       310        320        330        340        350        360 
KAVSLVLPQL KGKLNGIALR VPTPNVSVVD LVVNVAKKGI SAEDVNAAFR KAAEGPLKGI 

       370        380        390        400        410        420 
LDVCDVPLVS VDFRCSDVST TIDSSLTMVM GDDMVKVVAW YDNEWGYSQR VVDLAHLVAN 

       430        440        450 
KWPGTPKVGS GDPLEDFCET NPADEECKVY E

« Hide

References

[1]"Differential intron loss and endosymbiotic transfer of chloroplast glyceraldehyde-3-phosphate dehydrogenase genes to the nucleus."
Liaud M.-F., Zhang D.-X., Cerff R.
Proc. Natl. Acad. Sci. U.S.A. 87:8918-8922(1990) [PubMed: 2247465] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Strain: cv. Rosakrone.
Tissue: Seedling.
[2]"Cloning and sequence analysis of cDNAs encoding the cytosolic precursors of subunits GapA and GapB of chloroplast glyceraldehyde-3-phosphate dehydrogenase from pea and spinach."
Brinkmann H., Cerff R., Salomon M., Soll J.
Plant Mol. Biol. 13:81-94(1989) [PubMed: 2562762] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 5-451.
Strain: cv. Rosakrone.
Tissue: Seedling.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		M55147 Genomic DNA. Translation: AAA84543.1.
X15188 mRNA. Translation: CAA33262.1.
PIRDEPMNB. S16507.

3D structure databases

ProteinModelPortalP12859.
SMRP12859. Positions 85-424.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-29837N.

Proteomic databases

PRIDEP12859.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR003823. DUF_CP12.
IPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006424. Glyceraldehyde-3-P_DH_1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
Gene3DG3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHERPTHR10836. GAP_DH. 1 hit.
PfamPF02672. CP12. 1 hit.
PF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
[Graphical view]
PRINTSPR00078. G3PDHDRGNASE.
SMARTSM00846. Gp_dh_N. 1 hit.
[Graphical view]
TIGRFAMsTIGR01534. GAPDH-I. 1 hit.
PROSITEPS00071. GAPDH. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameG3PB_PEA
AccessionPrimary (citable) accession number: P12859
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: May 1, 1992
Last modified: January 25, 2012
This is version 93 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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