Reviewed, UniProtKB/Swiss-Prot P12859 (G3PB_PEA)

Names and origin

Protein names

Recommended name:
    Glyceraldehyde-3-phosphate dehydrogenase B, chloroplastic
    EC=1.2.1.13
Alternative name(s):
    NADP-dependent glyceraldehydephosphate dehydrogenase subunit B

Gene names

Name:	GAPB
Synonyms:	GPB1

Organism

Pisum sativum (Garden pea)

Taxonomic identifier

3888 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Fabales › Fabaceae › Papilionoideae › Fabeae › Pisum

Protein attributes

Sequence length	451 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate + phosphate + NADP(+) = 3-phospho-D-glyceroyl phosphate + NADPH.
Pathway	Carbohydrate biosynthesis; Calvin cycle.
Subunit structure	Tetramer of either four A chains (GAPDH 2) or two A and two B chains (GAPDH 1).
Subcellular location	Plastid › chloroplast.
Miscellaneous	Plants contain three forms of GAPDH: a cytosolic form which participates in glycolysis and two chloroplast forms which participates in photosynthesis. These three forms are encoded by distinct genes.
Sequence similarities	Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

Ontologies

Keywords
Biological process	Calvin cycle
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	glucose metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW reductive pentose-phosphate cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NAD binding Inferred from electronic annotation. Source: InterPro glyceraldehyde-3-phosphate dehydrogenase (NADP+) (phosphorylating) activity Inferred from electronic annotation. Source: EC glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 84	84	Chloroplast
	Chain	85 – 451	367	Glyceraldehyde-3-phosphate dehydrogenase B, chloroplastic		PRO_0000010424
Regions
	Nucleotide binding	95 – 96	2	NADP By similarity
	Region	238 – 240	3	Glyceraldehyde 3-phosphate binding By similarity
	Region	297 – 298	2	Glyceraldehyde 3-phosphate binding By similarity
Sites
	Active site	239	1	Nucleophile By similarity
	Binding site	119	1	NADP By similarity
	Binding site	164	1	NADP; via carbonyl oxygen By similarity
	Binding site	269	1	Glyceraldehyde 3-phosphate By similarity
	Binding site	284	1	Glyceraldehyde 3-phosphate By similarity
	Binding site	320	1	Glyceraldehyde 3-phosphate By similarity
	Binding site	403	1	NADP By similarity
	Site	266	1	Activates thiol group during catalysis By similarity
Experimental info
	Sequence conflict	255	1	F → S in CAA33262. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P12859-1 [UniParc]. Last modified May 1, 1992. Version 2. Checksum: C5D63CE37B19CE57	FASTA	451	48,097
	10 20 30 40 50 60 MATHAALAST RIPTNTRFPS KTSHSFPSQC ASKRLEVGEF SGLKSTSCIS YVHSARDSSF 70 80 90 100 110 120 YDVVAAQLTS KANGSTAVKG VTVAKLKVAI NGFGRIGRNF LRCWHGRKDS PLEVIVVNDS 130 140 150 160 170 180 GGVKNASHLL KYDSMLGTFK AEVKILNNET ITVDGKPIKV VSSRDPLKLP WAELGIDIVI 190 200 210 220 230 240 EGTGVFVDGP GAGKHIQAGA KKVIITAPAK GADIPTYVIG VNEQDYGHEV ADIISNASCT 250 260 270 280 290 300 TNCLAPFAKV LDEEFGIVKG TMTTTHSYTG DQRLLDASHR DLRRARAAAL NIVPTSTGAA 310 320 330 340 350 360 KAVSLVLPQL KGKLNGIALR VPTPNVSVVD LVVNVAKKGI SAEDVNAAFR KAAEGPLKGI 370 380 390 400 410 420 LDVCDVPLVS VDFRCSDVST TIDSSLTMVM GDDMVKVVAW YDNEWGYSQR VVDLAHLVAN 430 440 450 KWPGTPKVGS GDPLEDFCET NPADEECKVY E

References

[1]	"Differential intron loss and endosymbiotic transfer of chloroplast glyceraldehyde-3-phosphate dehydrogenase genes to the nucleus." Liaud M.-F., Zhang D.-X., Cerff R. Proc. Natl. Acad. Sci. U.S.A. 87:8918-8922(1990) [PubMed: 2247465] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: cv. Rosakrone. Tissue: Seedling.
[2]	"Cloning and sequence analysis of cDNAs encoding the cytosolic precursors of subunits GapA and GapB of chloroplast glyceraldehyde-3-phosphate dehydrogenase from pea and spinach." Brinkmann H., Cerff R., Salomon M., Soll J. Plant Mol. Biol. 13:81-94(1989) [PubMed: 2562762] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 5-451. Strain: cv. Rosakrone. Tissue: Seedling.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M55147 Genomic DNA. Translation: AAA84543.1. X15188 mRNA. Translation: CAA33262.1.
PIR	DEPMNB. S16507.
3D structure databases
HSSP	HSSP built from PDB template 1NBO based on UniProtKB P19866.
SMR	P12859. Positions 85-422.
ModBase	Search...
Family and domain databases
InterPro	IPR000173. GlycerAld_3-P_DHase. IPR006424. Glyceraldehyde-3-P_DHase_1. [Graphical view]
PANTHER	PTHR10836. GAP_DH. 1 hit.
Pfam	PF02800. Gp_dh_C. 1 hit. PF00044. Gp_dh_N. 1 hit. [Graphical view]
PRINTS	PR00078. G3PDHDRGNASE.
TIGRFAMs	TIGR01534. GAPDH-I. 1 hit.
PROSITE	PS00071. GAPDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G3PB_PEA

Accession

Primary (citable) accession number: P12859

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	May 1, 1992
Last modified:	November 25, 2008
This is version 72 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)