P12859 (G3PB_PEA) Reviewed, UniProtKB/Swiss-Prot
Last modified
January 25, 2012.
Version 93.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Glyceraldehyde-3-phosphate dehydrogenase B, chloroplastic EC=1.2.1.13 Alternative name(s): NADP-dependent glyceraldehydephosphate dehydrogenase subunit B | ||||
| Gene names |
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| Organism | Pisum sativum (Garden pea) | ||||
| Taxonomic identifier | 3888 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Fabeae › Pisum |
Protein attributes
| Sequence length | 451 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at transcript level |
General annotation (Comments)
| Catalytic activity | D-glyceraldehyde 3-phosphate + phosphate + NADP+ = 3-phospho-D-glyceroyl phosphate + NADPH. |
| Pathway | |
| Subunit structure | Tetramer of either four A chains (GAPDH 2) or two A and two B chains (GAPDH 1). |
| Subcellular location | |
| Miscellaneous | Plants contain three forms of GAPDH: a cytosolic form which participates in glycolysis and two chloroplast forms which participates in photosynthesis. These three forms are encoded by distinct genes. |
| Sequence similarities | Belongs to the glyceraldehyde-3-phosphate dehydrogenase family. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Calvin cycle |
| Cellular component | Chloroplast Plastid |
| Domain | Transit peptide |
| Ligand | NADP |
| Molecular function | Oxidoreductase |
| Gene Ontology (GO) | |
| Biological process | glucose metabolic process Inferred from electronic annotation. Source: InterPro reductive pentose-phosphate cycleInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | NAD binding Inferred from electronic annotation. Source: InterPro NADP bindingInferred from electronic annotation. Source: InterPro glyceraldehyde-3-phosphate dehydrogenase (NADP+) (phosphorylating) activityInferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Transit peptide | 1 – 84 | 84 | Chloroplast | ||||||
| Chain | 85 – 451 | 367 | Glyceraldehyde-3-phosphate dehydrogenase B, chloroplastic | PRO_0000010424 | |||||
Regions | |||||||||
| Nucleotide binding | 95 – 96 | 2 | NADP By similarity | ||||||
| Region | 238 – 240 | 3 | Glyceraldehyde 3-phosphate binding By similarity | ||||||
| Region | 297 – 298 | 2 | Glyceraldehyde 3-phosphate binding By similarity | ||||||
Sites | |||||||||
| Active site | 239 | 1 | Nucleophile By similarity | ||||||
| Binding site | 119 | 1 | NADP By similarity | ||||||
| Binding site | 164 | 1 | NADP; via carbonyl oxygen By similarity | ||||||
| Binding site | 269 | 1 | Glyceraldehyde 3-phosphate By similarity | ||||||
| Binding site | 284 | 1 | Glyceraldehyde 3-phosphate By similarity | ||||||
| Binding site | 320 | 1 | Glyceraldehyde 3-phosphate By similarity | ||||||
| Binding site | 403 | 1 | NADP By similarity | ||||||
| Site | 266 | 1 | Activates thiol group during catalysis By similarity | ||||||
Experimental info | |||||||||
| Sequence conflict | 255 | 1 | F → S in CAA33262. Ref.2 | ||||||
Sequences
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References
| [1] | "Differential intron loss and endosymbiotic transfer of chloroplast glyceraldehyde-3-phosphate dehydrogenase genes to the nucleus." Liaud M.-F., Zhang D.-X., Cerff R. Proc. Natl. Acad. Sci. U.S.A. 87:8918-8922(1990) [PubMed: 2247465] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: cv. Rosakrone. Tissue: Seedling. |
| [2] | "Cloning and sequence analysis of cDNAs encoding the cytosolic precursors of subunits GapA and GapB of chloroplast glyceraldehyde-3-phosphate dehydrogenase from pea and spinach." Brinkmann H., Cerff R., Salomon M., Soll J. Plant Mol. Biol. 13:81-94(1989) [PubMed: 2562762] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 5-451. Strain: cv. Rosakrone. Tissue: Seedling. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | M55147 Genomic DNA. Translation: AAA84543.1. X15188 mRNA. Translation: CAA33262.1. |
| PIR | DEPMNB. S16507. |
3D structure databases | |
| ProteinModelPortal | P12859. |
| SMR | P12859. Positions 85-424. |
| ModBase | Search... |
Protein-protein interaction databases | |
| DIP | DIP-29837N. |
Proteomic databases | |
| PRIDE | P12859. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Family and domain databases | |
| InterPro | IPR003823. DUF_CP12. IPR020831. GlycerAld/Erythrose_P_DH. IPR020830. GlycerAld_3-P_DH_AS. IPR020829. GlycerAld_3-P_DH_cat. IPR020828. GlycerAld_3-P_DH_NAD(P)-bd. IPR006424. Glyceraldehyde-3-P_DH_1. IPR016040. NAD(P)-bd_dom. [Graphical view] |
| Gene3D | G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. |
| PANTHER | PTHR10836. GAP_DH. 1 hit. |
| Pfam | PF02672. CP12. 1 hit. PF02800. Gp_dh_C. 1 hit. PF00044. Gp_dh_N. 1 hit. [Graphical view] |
| PRINTS | PR00078. G3PDHDRGNASE. |
| SMART | SM00846. Gp_dh_N. 1 hit. [Graphical view] |
| TIGRFAMs | TIGR01534. GAPDH-I. 1 hit. |
| PROSITE | PS00071. GAPDH. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | G3PB_PEA | ||||||||
| Accession | Primary (citable) accession number: P12859 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Plant Protein Annotation Program | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with