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Protein

Glyceraldehyde-3-phosphate dehydrogenase B, chloroplastic

Gene

GAPB

Organism
Pisum sativum (Garden pea)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Miscellaneous

Plants contain two types of GAPDH: cytosolic forms which participate in glycolysis and chloroplast forms which participate in photosynthesis. All the forms are encoded by distinct genes.

Catalytic activityi

D-glyceraldehyde 3-phosphate + phosphate + NADP+ = 3-phospho-D-glyceroyl phosphate + NADPH.

Pathwayi: Calvin cycle

This protein is involved in the pathway Calvin cycle, which is part of Carbohydrate biosynthesis.
View all proteins of this organism that are known to be involved in the pathway Calvin cycle and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei119NADPBy similarity1
Binding sitei164NADP; via carbonyl oxygenBy similarity1
Active sitei239NucleophilePROSITE-ProRule annotation1
Sitei266Activates thiol group during catalysisBy similarity1
Binding sitei269Glyceraldehyde 3-phosphateBy similarity1
Binding sitei284Glyceraldehyde 3-phosphateBy similarity1
Binding sitei320Glyceraldehyde 3-phosphateBy similarity1
Binding sitei403NADPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi95 – 96NADPBy similarity2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processCalvin cycle
LigandNADP

Enzyme and pathway databases

UniPathwayiUPA00116.

Names & Taxonomyi

Protein namesi
Recommended name:
Glyceraldehyde-3-phosphate dehydrogenase B, chloroplastic (EC:1.2.1.13)
Alternative name(s):
NADP-dependent glyceraldehydephosphate dehydrogenase subunit B
Gene namesi
Name:GAPB
Synonyms:GPB1
OrganismiPisum sativum (Garden pea)
Taxonomic identifieri3888 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeFabeaePisum

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 84ChloroplastAdd BLAST84
ChainiPRO_000001042485 – 451Glyceraldehyde-3-phosphate dehydrogenase B, chloroplasticAdd BLAST367

Proteomic databases

PRIDEiP12859.

Interactioni

Subunit structurei

Tetramer of either four A chains (GAPDH 2) or two A and two B chains (GAPDH 1).By similarity

Protein-protein interaction databases

DIPiDIP-29837N.

Structurei

3D structure databases

ProteinModelPortaliP12859.
SMRiP12859.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni238 – 240Glyceraldehyde 3-phosphate bindingBy similarity3
Regioni297 – 298Glyceraldehyde 3-phosphate bindingBy similarity2

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Family and domain databases

InterProiView protein in InterPro
IPR003823. DUF_CP12.
IPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006424. Glyceraldehyde-3-P_DH_1.
IPR016040. NAD(P)-bd_dom.
PfamiView protein in Pfam
PF02672. CP12. 1 hit.
PF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
PRINTSiPR00078. G3PDHDRGNASE.
SMARTiView protein in SMART
SM00846. Gp_dh_N. 1 hit.
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01534. GAPDH-I. 1 hit.
PROSITEiView protein in PROSITE
PS00071. GAPDH. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P12859-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATHAALAST RIPTNTRFPS KTSHSFPSQC ASKRLEVGEF SGLKSTSCIS
60 70 80 90 100
YVHSARDSSF YDVVAAQLTS KANGSTAVKG VTVAKLKVAI NGFGRIGRNF
110 120 130 140 150
LRCWHGRKDS PLEVIVVNDS GGVKNASHLL KYDSMLGTFK AEVKILNNET
160 170 180 190 200
ITVDGKPIKV VSSRDPLKLP WAELGIDIVI EGTGVFVDGP GAGKHIQAGA
210 220 230 240 250
KKVIITAPAK GADIPTYVIG VNEQDYGHEV ADIISNASCT TNCLAPFAKV
260 270 280 290 300
LDEEFGIVKG TMTTTHSYTG DQRLLDASHR DLRRARAAAL NIVPTSTGAA
310 320 330 340 350
KAVSLVLPQL KGKLNGIALR VPTPNVSVVD LVVNVAKKGI SAEDVNAAFR
360 370 380 390 400
KAAEGPLKGI LDVCDVPLVS VDFRCSDVST TIDSSLTMVM GDDMVKVVAW
410 420 430 440 450
YDNEWGYSQR VVDLAHLVAN KWPGTPKVGS GDPLEDFCET NPADEECKVY

E
Length:451
Mass (Da):48,097
Last modified:May 1, 1992 - v2
Checksum:iC5D63CE37B19CE57
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti255F → S in CAA33262 (PubMed:2562762).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M55147 Genomic DNA. Translation: AAA84543.1.
X15188 mRNA. Translation: CAA33262.1.
PIRiS16507. DEPMNB.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M55147 Genomic DNA. Translation: AAA84543.1.
X15188 mRNA. Translation: CAA33262.1.
PIRiS16507. DEPMNB.

3D structure databases

ProteinModelPortaliP12859.
SMRiP12859.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-29837N.

Proteomic databases

PRIDEiP12859.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00116.

Family and domain databases

InterProiView protein in InterPro
IPR003823. DUF_CP12.
IPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006424. Glyceraldehyde-3-P_DH_1.
IPR016040. NAD(P)-bd_dom.
PfamiView protein in Pfam
PF02672. CP12. 1 hit.
PF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
PRINTSiPR00078. G3PDHDRGNASE.
SMARTiView protein in SMART
SM00846. Gp_dh_N. 1 hit.
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01534. GAPDH-I. 1 hit.
PROSITEiView protein in PROSITE
PS00071. GAPDH. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiG3PB_PEA
AccessioniPrimary (citable) accession number: P12859
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: May 1, 1992
Last modified: May 10, 2017
This is version 114 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.