Reviewed, UniProtKB/Swiss-Prot P12858 (G3PA_PEA)

Names and origin

Protein names

Recommended name:
    Glyceraldehyde-3-phosphate dehydrogenase A, chloroplastic
    EC=1.2.1.13
Alternative name(s):
    NADP-dependent glyceraldehydephosphate dehydrogenase subunit A

Gene names

Name:	GAPA
Synonyms:	GPA1

Organism

Pisum sativum (Garden pea)

Taxonomic identifier

3888 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Fabales › Fabaceae › Papilionoideae › Fabeae › Pisum

Protein attributes

Sequence length	405 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate + phosphate + NADP(+) = 3-phospho-D-glyceroyl phosphate + NADPH.
Pathway	Carbohydrate biosynthesis; Calvin cycle.
Subunit structure	Tetramer of either four A chains (GAPDH 2) or two A and two B chains (GAPDH 1).
Subcellular location	Plastid › chloroplast.
Miscellaneous	Plants contain three forms of GAPDH: a cytosolic form which participates in glycolysis and two chloroplast forms which participates in photosynthesis. These three forms are encoded by distinct genes.
Sequence similarities	Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

Ontologies

Keywords
Biological process	Calvin cycle
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	glucose metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW reductive pentose-phosphate cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NAD binding Inferred from electronic annotation. Source: InterPro glyceraldehyde-3-phosphate dehydrogenase (NADP+) (phosphorylating) activity Inferred from electronic annotation. Source: EC glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 68	68	Chloroplast
	Chain	69 – 405	337	Glyceraldehyde-3-phosphate dehydrogenase A, chloroplastic		PRO_0000010423
Regions
	Nucleotide binding	80 – 81	2	NADP By similarity
	Region	221 – 223	3	Glyceraldehyde 3-phosphate binding By similarity
	Region	280 – 281	2	Glyceraldehyde 3-phosphate binding By similarity
Sites
	Active site	222	1	Nucleophile By similarity
	Binding site	104	1	NADP By similarity
	Binding site	149	1	NADP; via carbonyl oxygen By similarity
	Binding site	252	1	Glyceraldehyde 3-phosphate By similarity
	Binding site	267	1	Glyceraldehyde 3-phosphate By similarity
	Binding site	303	1	Glyceraldehyde 3-phosphate By similarity
	Binding site	385	1	NADP By similarity
	Site	249	1	Activates thiol group during catalysis By similarity
Experimental info
	Sequence conflict	194	1	G → R in CAA33264. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P12858-1 [UniParc]. Last modified May 1, 1992. Version 2. Checksum: 667FD75FD3EA6430	FASTA	405	43,338
	10 20 30 40 50 60 MASATFSVAK PAIKANGKGF SEFSGLRNSS RHLPFSRKSS DDFHSLVTFQ TNAVGSSGGH 70 80 90 100 110 120 KKSLVVEAKQ LKVAINGFGR IGRNFLRCWH GRKDSPLDVI AINDTGGVKQ ASHLLKYDST 130 140 150 160 170 180 LGIFDADVKP VGTDGISVDG KVIKVVSDRN PANLPWKELG IDLVIEGTGV FVDREGAGRH 190 200 210 220 230 240 ITAGAKKVLI TAPGKGDIPT YVVGVNADAY THADDIISNA SCTTNCLAPF VKVLDQKFGI 250 260 270 280 290 300 IKGTMTTTHS YTGDQRLLDA SHRDLRRARA AALNIVPTST GAAKAVALVL PTLKGKLNGI 310 320 330 340 350 360 ALRVPTPNVS VVDLVVQVSK KTFAEEVNEA FRESAAKELT GILSVCDEPL VSVDFRCTDV 370 380 390 400 SSTVDSSLTM VMGDDLVKVI AWYDNEWGYS QRVVDLADIV ANNWK

References

[1]	"Differential intron loss and endosymbiotic transfer of chloroplast glyceraldehyde-3-phosphate dehydrogenase genes to the nucleus." Liaud M.-F., Zhang D.-X., Cerff R. Proc. Natl. Acad. Sci. U.S.A. 87:8918-8922(1990) [PubMed: 2247465] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Rosakrone. Tissue: Seedling.
[2]	"Cloning and sequence analysis of cDNAs encoding the cytosolic precursors of subunits GapA and GapB of chloroplast glyceraldehyde-3-phosphate dehydrogenase from pea and spinach." Brinkmann H., Cerff R., Salomon M., Soll J. Plant Mol. Biol. 13:81-94(1989) [PubMed: 2562762] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Rosakrone. Tissue: Seedling.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X52148 Genomic DNA. Translation: CAA36396.1. X15190 mRNA. Translation: CAA33264.1.
PIR	DEPMNA. S14243.
3D structure databases
HSSP	HSSP built from PDB template 1NBO based on UniProtKB P19866.
SMR	P12858. Positions 70-405.
ModBase	Search...
Family and domain databases
InterPro	IPR000173. GlycerAld_3-P_DHase. IPR006424. Glyceraldehyde-3-P_DHase_1. [Graphical view]
PANTHER	PTHR10836. GAP_DH. 1 hit.
Pfam	PF02800. Gp_dh_C. 1 hit. PF00044. Gp_dh_N. 1 hit. [Graphical view]
PIRSF	PIRSF000149. GAP_DH. 1 hit.
PRINTS	PR00078. G3PDHDRGNASE.
TIGRFAMs	TIGR01534. GAPDH-I. 1 hit.
PROSITE	PS00071. GAPDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G3PA_PEA

Accession

Primary (citable) accession number: P12858

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	May 1, 1992
Last modified:	November 25, 2008
This is version 70 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)