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Protein

Glyceraldehyde-3-phosphate dehydrogenase A, chloroplastic

Gene

GAPA

Organism
Pisum sativum (Garden pea)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

D-glyceraldehyde 3-phosphate + phosphate + NADP+ = 3-phospho-D-glyceroyl phosphate + NADPH.

Pathwayi: Calvin cycle

This protein is involved in the pathway Calvin cycle, which is part of Carbohydrate biosynthesis.
View all proteins of this organism that are known to be involved in the pathway Calvin cycle and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei104NADPBy similarity1
Binding sitei149NADP; via carbonyl oxygenBy similarity1
Active sitei222NucleophilePROSITE-ProRule annotation1
Sitei249Activates thiol group during catalysisBy similarity1
Binding sitei252Glyceraldehyde 3-phosphateBy similarity1
Binding sitei267Glyceraldehyde 3-phosphateBy similarity1
Binding sitei303Glyceraldehyde 3-phosphateBy similarity1
Binding sitei385NADPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi80 – 81NADPBy similarity2

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Calvin cycle

Keywords - Ligandi

NADP

Enzyme and pathway databases

UniPathwayiUPA00116.

Names & Taxonomyi

Protein namesi
Recommended name:
Glyceraldehyde-3-phosphate dehydrogenase A, chloroplastic (EC:1.2.1.13)
Alternative name(s):
NADP-dependent glyceraldehydephosphate dehydrogenase subunit A
Gene namesi
Name:GAPA
Synonyms:GPA1
OrganismiPisum sativum (Garden pea)
Taxonomic identifieri3888 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeFabeaePisum

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 68ChloroplastAdd BLAST68
ChainiPRO_000001042369 – 405Glyceraldehyde-3-phosphate dehydrogenase A, chloroplasticAdd BLAST337

Proteomic databases

PRIDEiP12858.

Interactioni

Subunit structurei

Tetramer of either four A chains (GAPDH 2) or two A and two B chains (GAPDH 1).By similarity

Protein-protein interaction databases

DIPiDIP-29836N.

Structurei

3D structure databases

ProteinModelPortaliP12858.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni221 – 223Glyceraldehyde 3-phosphate bindingBy similarity3
Regioni280 – 281Glyceraldehyde 3-phosphate bindingBy similarity2

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006424. Glyceraldehyde-3-P_DH_1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR10836. PTHR10836. 1 hit.
PfamiPF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
[Graphical view]
PRINTSiPR00078. G3PDHDRGNASE.
SMARTiSM00846. Gp_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01534. GAPDH-I. 1 hit.
PROSITEiPS00071. GAPDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P12858-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASATFSVAK PAIKANGKGF SEFSGLRNSS RHLPFSRKSS DDFHSLVTFQ
60 70 80 90 100
TNAVGSSGGH KKSLVVEAKQ LKVAINGFGR IGRNFLRCWH GRKDSPLDVI
110 120 130 140 150
AINDTGGVKQ ASHLLKYDST LGIFDADVKP VGTDGISVDG KVIKVVSDRN
160 170 180 190 200
PANLPWKELG IDLVIEGTGV FVDREGAGRH ITAGAKKVLI TAPGKGDIPT
210 220 230 240 250
YVVGVNADAY THADDIISNA SCTTNCLAPF VKVLDQKFGI IKGTMTTTHS
260 270 280 290 300
YTGDQRLLDA SHRDLRRARA AALNIVPTST GAAKAVALVL PTLKGKLNGI
310 320 330 340 350
ALRVPTPNVS VVDLVVQVSK KTFAEEVNEA FRESAAKELT GILSVCDEPL
360 370 380 390 400
VSVDFRCTDV SSTVDSSLTM VMGDDLVKVI AWYDNEWGYS QRVVDLADIV

ANNWK
Length:405
Mass (Da):43,338
Last modified:May 1, 1992 - v2
Checksum:i667FD75FD3EA6430
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti194G → R in CAA33264 (PubMed:2562762).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52148 Genomic DNA. Translation: CAA36396.1.
X15190 mRNA. Translation: CAA33264.1.
PIRiS14243. DEPMNA.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52148 Genomic DNA. Translation: CAA36396.1.
X15190 mRNA. Translation: CAA33264.1.
PIRiS14243. DEPMNA.

3D structure databases

ProteinModelPortaliP12858.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-29836N.

Proteomic databases

PRIDEiP12858.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00116.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006424. Glyceraldehyde-3-P_DH_1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR10836. PTHR10836. 1 hit.
PfamiPF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
[Graphical view]
PRINTSiPR00078. G3PDHDRGNASE.
SMARTiSM00846. Gp_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01534. GAPDH-I. 1 hit.
PROSITEiPS00071. GAPDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiG3PA_PEA
AccessioniPrimary (citable) accession number: P12858
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: May 1, 1992
Last modified: October 5, 2016
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Plants contain two types of GAPDH: cytosolic forms which participate in glycolysis and chloroplast forms which participate in photosynthesis. All the forms are encoded by distinct genes.

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.