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P12851

- XYLA_ACTM4

UniProt

P12851 - XYLA_ACTM4

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Protein

Xylose isomerase

Gene
xylA, XI, AMIS_10350
Organism
Actinoplanes missouriensis (strain ATCC 14538 / DSM 43046 / CBS 188.64 / JCM 3121 / NCIMB 12654 / NBRC 102363 / 431)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

D-xylopyranose = D-xylulose.UniRule annotation

Cofactori

Binds 2 magnesium ions per subunit.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei54 – 541
Active sitei57 – 571 By similarity
Metal bindingi181 – 1811Magnesium 1
Metal bindingi217 – 2171Magnesium 1
Metal bindingi217 – 2171Magnesium 2
Metal bindingi220 – 2201Magnesium 2
Metal bindingi245 – 2451Magnesium 1
Metal bindingi255 – 2551Magnesium 2
Metal bindingi257 – 2571Magnesium 2
Metal bindingi292 – 2921Magnesium 1

GO - Molecular functioni

  1. magnesium ion binding Source: UniProtKB-HAMAP
  2. xylose isomerase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. D-xylose metabolic process Source: UniProtKB-HAMAP
  2. pentose-phosphate shunt Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Carbohydrate metabolism, Pentose shunt, Xylose metabolism

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciAMIS512565:GL7J-1062-MONOMER.
SABIO-RKP12851.

Names & Taxonomyi

Protein namesi
Recommended name:
Xylose isomerase (EC:5.3.1.5)
Gene namesi
Name:xylA
Synonyms:XI
Ordered Locus Names:AMIS_10350
OrganismiActinoplanes missouriensis (strain ATCC 14538 / DSM 43046 / CBS 188.64 / JCM 3121 / NCIMB 12654 / NBRC 102363 / 431)
Taxonomic identifieri512565 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesMicromonosporineaeMicromonosporaceaeActinoplanes
ProteomesiUP000007882: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedUniRule annotation
Chaini2 – 394393Xylose isomeraseUniRule annotationPRO_0000195757Add
BLAST

Interactioni

Subunit structurei

Homotetramer.

Structurei

Secondary structure

1
394
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi7 – 93
Beta strandi11 – 144
Helixi15 – 184
Helixi36 – 4611
Beta strandi49 – 524
Helixi55 – 584
Helixi65 – 8218
Beta strandi88 – 903
Beta strandi94 – 963
Helixi97 – 993
Helixi109 – 12921
Beta strandi133 – 1375
Beta strandi141 – 1455
Helixi146 – 1483
Helixi151 – 17222
Beta strandi177 – 1815
Beta strandi184 – 19310
Helixi196 – 2038
Beta strandi206 – 2083
Helixi209 – 2113
Beta strandi212 – 2143
Helixi218 – 2225
Turni223 – 2253
Helixi228 – 23811
Beta strandi244 – 2463
Beta strandi251 – 2544
Beta strandi262 – 2643
Helixi265 – 27612
Helixi279 – 2813
Beta strandi289 – 2913
Helixi301 – 32727
Helixi329 – 3379
Helixi341 – 3444
Helixi354 – 3596
Helixi361 – 3633
Turni364 – 3663
Helixi369 – 3735
Helixi379 – 39012

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1BHWX-ray4.10A/B/C/D2-394[»]
1XIMX-ray2.20A/B/C/D2-394[»]
1XINX-ray2.40A/B/C/D2-394[»]
2XIMX-ray2.30A/B/C/D2-394[»]
2XINX-ray2.30A/B/C/D2-394[»]
3XIMX-ray2.30A/B/C/D2-394[»]
3XINX-ray2.30A/B/C/D2-394[»]
4XIMX-ray2.30A/B/C/D2-394[»]
5XIMX-ray2.60A/B/C/D2-394[»]
5XINX-ray2.30A/B/C/D2-394[»]
6XIMX-ray2.50A/B/C/D2-394[»]
7XIMX-ray2.40A/B/C/D2-394[»]
8XIMX-ray2.40A/B/C/D2-394[»]
9XIMX-ray2.40A/B/C/D2-394[»]
ProteinModelPortaliP12851.
SMRiP12851. Positions 3-394.

Miscellaneous databases

EvolutionaryTraceiP12851.

Family & Domainsi

Sequence similaritiesi

Belongs to the xylose isomerase family.

Phylogenomic databases

KOiK01805.
OMAiDLIPIDA.

Family and domain databases

Gene3Di3.20.20.150. 1 hit.
HAMAPiMF_00455. Xylose_isom_A.
InterProiIPR013022. Xyl_isomerase-like_TIM-brl.
IPR013453. XylA_actinobac.
IPR001998. Xylose_isomerase.
[Graphical view]
PfamiPF01261. AP_endonuc_2. 1 hit.
[Graphical view]
PRINTSiPR00688. XYLOSISMRASE.
SUPFAMiSSF51658. SSF51658. 1 hit.
TIGRFAMsiTIGR02631. xylA_Arthro. 1 hit.
PROSITEiPS51415. XYLOSE_ISOMERASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P12851-1 [UniParc]FASTAAdd to Basket

« Hide

MSVQATREDK FSFGLWTVGW QARDAFGDAT RTALDPVEAV HKLAEIGAYG    50
ITFHDDDLVP FGSDAQTRDG IIAGFKKALD ETGLIVPMVT TNLFTHPVFK 100
DGGFTSNDRS VRRYAIRKVL RQMDLGAELG AKTLVLWGGR EGAEYDSAKD 150
VSAALDRYRE ALNLLAQYSE DRGYGLRFAI EPKPNEPRGD ILLPTAGHAI 200
AFVQELERPE LFGINPETGH EQMSNLNFTQ GIAQALWHKK LFHIDLNGQH 250
GPKFDQDLVF GHGDLLNAFS LVDLLENGPD GAPAYDGPRH FDYKPSRTED 300
YDGVWESAKA NIRMYLLLKE RAKAFRADPE VQEALAASKV AELKTPTLNP 350
GEGYAELLAD RSAFEDYDAD AVGAKGFGFV KLNQLAIEHL LGAR 394
Length:394
Mass (Da):43,499
Last modified:January 23, 2007 - v3
Checksum:i764B1FC35AE7A780
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X16042 Genomic DNA. Translation: CAA34164.1.
AP012319 Genomic DNA. Translation: BAL86255.1.
PIRiS05998. ISMAXM.
RefSeqiWP_014441152.1. NC_017093.1.
YP_005460771.1. NC_017093.1.

Genome annotation databases

EnsemblBacteriaiBAL86255; BAL86255; AMIS_10350.
GeneIDi12051265.
KEGGiams:AMIS_10350.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X16042 Genomic DNA. Translation: CAA34164.1 .
AP012319 Genomic DNA. Translation: BAL86255.1 .
PIRi S05998. ISMAXM.
RefSeqi WP_014441152.1. NC_017093.1.
YP_005460771.1. NC_017093.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1BHW X-ray 4.10 A/B/C/D 2-394 [» ]
1XIM X-ray 2.20 A/B/C/D 2-394 [» ]
1XIN X-ray 2.40 A/B/C/D 2-394 [» ]
2XIM X-ray 2.30 A/B/C/D 2-394 [» ]
2XIN X-ray 2.30 A/B/C/D 2-394 [» ]
3XIM X-ray 2.30 A/B/C/D 2-394 [» ]
3XIN X-ray 2.30 A/B/C/D 2-394 [» ]
4XIM X-ray 2.30 A/B/C/D 2-394 [» ]
5XIM X-ray 2.60 A/B/C/D 2-394 [» ]
5XIN X-ray 2.30 A/B/C/D 2-394 [» ]
6XIM X-ray 2.50 A/B/C/D 2-394 [» ]
7XIM X-ray 2.40 A/B/C/D 2-394 [» ]
8XIM X-ray 2.40 A/B/C/D 2-394 [» ]
9XIM X-ray 2.40 A/B/C/D 2-394 [» ]
ProteinModelPortali P12851.
SMRi P12851. Positions 3-394.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAL86255 ; BAL86255 ; AMIS_10350 .
GeneIDi 12051265.
KEGGi ams:AMIS_10350.

Phylogenomic databases

KOi K01805.
OMAi DLIPIDA.

Enzyme and pathway databases

BioCyci AMIS512565:GL7J-1062-MONOMER.
SABIO-RK P12851.

Miscellaneous databases

EvolutionaryTracei P12851.

Family and domain databases

Gene3Di 3.20.20.150. 1 hit.
HAMAPi MF_00455. Xylose_isom_A.
InterProi IPR013022. Xyl_isomerase-like_TIM-brl.
IPR013453. XylA_actinobac.
IPR001998. Xylose_isomerase.
[Graphical view ]
Pfami PF01261. AP_endonuc_2. 1 hit.
[Graphical view ]
PRINTSi PR00688. XYLOSISMRASE.
SUPFAMi SSF51658. SSF51658. 1 hit.
TIGRFAMsi TIGR02631. xylA_Arthro. 1 hit.
PROSITEi PS51415. XYLOSE_ISOMERASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Xylose isomerase from Actinoplanes missouriensis: primary structure of the gene and the protein."
    Amore R., Hollenberg C.P.
    Nucleic Acids Res. 17:7515-7515(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 14538 / DSM 43046 / CBS 188.64 / JCM 3121 / NCIMB 12654 / NBRC 102363 / 431.
  2. "Complete genome sequence of Actinoplanes missouriensis 431 (= NBRC 102363)."
    Ohnishi Y., Ishikawa J., Sekine M., Hosoyama A., Harada T., Narita H., Hata T., Konno Y., Tutikane K., Fujita N., Horinouchi S., Hayakawa M.
    Submitted (FEB-2012) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 14538 / DSM 43046 / CBS 188.64 / JCM 3121 / NCIMB 12654 / NBRC 102363 / 431.
  3. "Structural analysis of the 2.8 A model of Xylose isomerase from Actinoplanes missouriensis."
    Rey F., Jenkins J., Janin J., Lasters I., Alard P., Claessens M., Matthyssens G., Wodak S.J.
    Proteins 4:165-172(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS).
  4. "Protein engineering of xylose (glucose) isomerase from Actinoplanes missouriensis. 1. Crystallography and site-directed mutagenesis of metal binding sites."
    Jenkins J., Janin J., Rey F., Chiadmi M., van Tilbeurgh H., Lasters I., de Maeyer M., van Belle D., Wodak S.J., Lauwereys M., Stanssens P., Mrabet N.T., Snauwaert J., Matthyssens G., Lambeir A.-M.
    Biochemistry 31:5449-5458(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS).
  5. "Multiwavelength anomalous solvent contrast (MASC): derivation of envelope structure-factor amplitudes and comparison with model values."
    Ramin M., Shepard W., Fourme R., Kahn R.
    Acta Crystallogr. D 55:157-167(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (4.1 ANGSTROMS).
  6. "Protein engineering of xylose (glucose) isomerase from Actinoplanes missouriensis. 2. Site-directed mutagenesis of the xylose binding site."
    Lambeir A.-M., Lauwereys M., Stanssens P., Mrabet N.T., Snauwaert J., van Tilbeurgh H., Matthyssens G., Lasters I., de Maeyer M., Wodak S.J., Jenkins J., Chiadmi M., Janin J.
    Biochemistry 31:5459-5466(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS.
  7. "Protein engineering of xylose (glucose) isomerase from Actinoplanes missouriensis. 3. Changing metal specificity and the pH profile by site-directed mutagenesis."
    van Tilbeurgh H., Jenkins J., Chiadmi M., Janin J., Wodak S.J., Mrabet N.T., Lambeir A.-M.
    Biochemistry 31:5467-5472(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS.

Entry informationi

Entry nameiXYLA_ACTM4
AccessioniPrimary (citable) accession number: P12851
Secondary accession number(s): I0GZR8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: January 23, 2007
Last modified: September 3, 2014
This is version 108 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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