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P12851

- XYLA_ACTM4

UniProt

P12851 - XYLA_ACTM4

Protein

Xylose isomerase

Gene

xylA

Organism
Actinoplanes missouriensis (strain ATCC 14538 / DSM 43046 / CBS 188.64 / JCM 3121 / NCIMB 12654 / NBRC 102363 / 431)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 109 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    D-xylopyranose = D-xylulose.

    Cofactori

    Binds 2 magnesium ions per subunit.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei54 – 541
    Active sitei57 – 571By similarity
    Metal bindingi181 – 1811Magnesium 1
    Metal bindingi217 – 2171Magnesium 1
    Metal bindingi217 – 2171Magnesium 2
    Metal bindingi220 – 2201Magnesium 2
    Metal bindingi245 – 2451Magnesium 1
    Metal bindingi255 – 2551Magnesium 2
    Metal bindingi257 – 2571Magnesium 2
    Metal bindingi292 – 2921Magnesium 1

    GO - Molecular functioni

    1. magnesium ion binding Source: UniProtKB-HAMAP
    2. xylose isomerase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. D-xylose metabolic process Source: UniProtKB-HAMAP
    2. pentose-phosphate shunt Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Isomerase

    Keywords - Biological processi

    Carbohydrate metabolism, Pentose shunt, Xylose metabolism

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciAMIS512565:GL7J-1062-MONOMER.
    SABIO-RKP12851.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Xylose isomerase (EC:5.3.1.5)
    Gene namesi
    Name:xylA
    Synonyms:XI
    Ordered Locus Names:AMIS_10350
    OrganismiActinoplanes missouriensis (strain ATCC 14538 / DSM 43046 / CBS 188.64 / JCM 3121 / NCIMB 12654 / NBRC 102363 / 431)
    Taxonomic identifieri512565 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesMicromonosporineaeMicromonosporaceaeActinoplanes
    ProteomesiUP000007882: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed
    Chaini2 – 394393Xylose isomerasePRO_0000195757Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.

    Structurei

    Secondary structure

    1
    394
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi7 – 93
    Beta strandi11 – 144
    Helixi15 – 184
    Helixi36 – 4611
    Beta strandi49 – 524
    Helixi55 – 584
    Helixi65 – 8218
    Beta strandi88 – 903
    Beta strandi94 – 963
    Helixi97 – 993
    Helixi109 – 12921
    Beta strandi133 – 1375
    Beta strandi141 – 1455
    Helixi146 – 1483
    Helixi151 – 17222
    Beta strandi177 – 1815
    Beta strandi184 – 19310
    Helixi196 – 2038
    Beta strandi206 – 2083
    Helixi209 – 2113
    Beta strandi212 – 2143
    Helixi218 – 2225
    Turni223 – 2253
    Helixi228 – 23811
    Beta strandi244 – 2463
    Beta strandi251 – 2544
    Beta strandi262 – 2643
    Helixi265 – 27612
    Helixi279 – 2813
    Beta strandi289 – 2913
    Helixi301 – 32727
    Helixi329 – 3379
    Helixi341 – 3444
    Helixi354 – 3596
    Helixi361 – 3633
    Turni364 – 3663
    Helixi369 – 3735
    Helixi379 – 39012

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1BHWX-ray4.10A/B/C/D2-394[»]
    1XIMX-ray2.20A/B/C/D2-394[»]
    1XINX-ray2.40A/B/C/D2-394[»]
    2XIMX-ray2.30A/B/C/D2-394[»]
    2XINX-ray2.30A/B/C/D2-394[»]
    3XIMX-ray2.30A/B/C/D2-394[»]
    3XINX-ray2.30A/B/C/D2-394[»]
    4XIMX-ray2.30A/B/C/D2-394[»]
    5XIMX-ray2.60A/B/C/D2-394[»]
    5XINX-ray2.30A/B/C/D2-394[»]
    6XIMX-ray2.50A/B/C/D2-394[»]
    7XIMX-ray2.40A/B/C/D2-394[»]
    8XIMX-ray2.40A/B/C/D2-394[»]
    9XIMX-ray2.40A/B/C/D2-394[»]
    ProteinModelPortaliP12851.
    SMRiP12851. Positions 3-394.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP12851.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the xylose isomerase family.Curated

    Phylogenomic databases

    KOiK01805.
    OMAiDLIPIDA.

    Family and domain databases

    Gene3Di3.20.20.150. 1 hit.
    HAMAPiMF_00455. Xylose_isom_A.
    InterProiIPR013022. Xyl_isomerase-like_TIM-brl.
    IPR013453. XylA_actinobac.
    IPR001998. Xylose_isomerase.
    [Graphical view]
    PfamiPF01261. AP_endonuc_2. 1 hit.
    [Graphical view]
    PRINTSiPR00688. XYLOSISMRASE.
    SUPFAMiSSF51658. SSF51658. 1 hit.
    TIGRFAMsiTIGR02631. xylA_Arthro. 1 hit.
    PROSITEiPS51415. XYLOSE_ISOMERASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P12851-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSVQATREDK FSFGLWTVGW QARDAFGDAT RTALDPVEAV HKLAEIGAYG    50
    ITFHDDDLVP FGSDAQTRDG IIAGFKKALD ETGLIVPMVT TNLFTHPVFK 100
    DGGFTSNDRS VRRYAIRKVL RQMDLGAELG AKTLVLWGGR EGAEYDSAKD 150
    VSAALDRYRE ALNLLAQYSE DRGYGLRFAI EPKPNEPRGD ILLPTAGHAI 200
    AFVQELERPE LFGINPETGH EQMSNLNFTQ GIAQALWHKK LFHIDLNGQH 250
    GPKFDQDLVF GHGDLLNAFS LVDLLENGPD GAPAYDGPRH FDYKPSRTED 300
    YDGVWESAKA NIRMYLLLKE RAKAFRADPE VQEALAASKV AELKTPTLNP 350
    GEGYAELLAD RSAFEDYDAD AVGAKGFGFV KLNQLAIEHL LGAR 394
    Length:394
    Mass (Da):43,499
    Last modified:January 23, 2007 - v3
    Checksum:i764B1FC35AE7A780
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X16042 Genomic DNA. Translation: CAA34164.1.
    AP012319 Genomic DNA. Translation: BAL86255.1.
    PIRiS05998. ISMAXM.
    RefSeqiWP_014441152.1. NC_017093.1.
    YP_005460771.1. NC_017093.1.

    Genome annotation databases

    EnsemblBacteriaiBAL86255; BAL86255; AMIS_10350.
    GeneIDi12051265.
    KEGGiams:AMIS_10350.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X16042 Genomic DNA. Translation: CAA34164.1 .
    AP012319 Genomic DNA. Translation: BAL86255.1 .
    PIRi S05998. ISMAXM.
    RefSeqi WP_014441152.1. NC_017093.1.
    YP_005460771.1. NC_017093.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1BHW X-ray 4.10 A/B/C/D 2-394 [» ]
    1XIM X-ray 2.20 A/B/C/D 2-394 [» ]
    1XIN X-ray 2.40 A/B/C/D 2-394 [» ]
    2XIM X-ray 2.30 A/B/C/D 2-394 [» ]
    2XIN X-ray 2.30 A/B/C/D 2-394 [» ]
    3XIM X-ray 2.30 A/B/C/D 2-394 [» ]
    3XIN X-ray 2.30 A/B/C/D 2-394 [» ]
    4XIM X-ray 2.30 A/B/C/D 2-394 [» ]
    5XIM X-ray 2.60 A/B/C/D 2-394 [» ]
    5XIN X-ray 2.30 A/B/C/D 2-394 [» ]
    6XIM X-ray 2.50 A/B/C/D 2-394 [» ]
    7XIM X-ray 2.40 A/B/C/D 2-394 [» ]
    8XIM X-ray 2.40 A/B/C/D 2-394 [» ]
    9XIM X-ray 2.40 A/B/C/D 2-394 [» ]
    ProteinModelPortali P12851.
    SMRi P12851. Positions 3-394.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAL86255 ; BAL86255 ; AMIS_10350 .
    GeneIDi 12051265.
    KEGGi ams:AMIS_10350.

    Phylogenomic databases

    KOi K01805.
    OMAi DLIPIDA.

    Enzyme and pathway databases

    BioCyci AMIS512565:GL7J-1062-MONOMER.
    SABIO-RK P12851.

    Miscellaneous databases

    EvolutionaryTracei P12851.

    Family and domain databases

    Gene3Di 3.20.20.150. 1 hit.
    HAMAPi MF_00455. Xylose_isom_A.
    InterProi IPR013022. Xyl_isomerase-like_TIM-brl.
    IPR013453. XylA_actinobac.
    IPR001998. Xylose_isomerase.
    [Graphical view ]
    Pfami PF01261. AP_endonuc_2. 1 hit.
    [Graphical view ]
    PRINTSi PR00688. XYLOSISMRASE.
    SUPFAMi SSF51658. SSF51658. 1 hit.
    TIGRFAMsi TIGR02631. xylA_Arthro. 1 hit.
    PROSITEi PS51415. XYLOSE_ISOMERASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Xylose isomerase from Actinoplanes missouriensis: primary structure of the gene and the protein."
      Amore R., Hollenberg C.P.
      Nucleic Acids Res. 17:7515-7515(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 14538 / DSM 43046 / CBS 188.64 / JCM 3121 / NCIMB 12654 / NBRC 102363 / 431.
    2. "Complete genome sequence of Actinoplanes missouriensis 431 (= NBRC 102363)."
      Ohnishi Y., Ishikawa J., Sekine M., Hosoyama A., Harada T., Narita H., Hata T., Konno Y., Tutikane K., Fujita N., Horinouchi S., Hayakawa M.
      Submitted (FEB-2012) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 14538 / DSM 43046 / CBS 188.64 / JCM 3121 / NCIMB 12654 / NBRC 102363 / 431.
    3. "Structural analysis of the 2.8 A model of Xylose isomerase from Actinoplanes missouriensis."
      Rey F., Jenkins J., Janin J., Lasters I., Alard P., Claessens M., Matthyssens G., Wodak S.J.
      Proteins 4:165-172(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS).
    4. "Protein engineering of xylose (glucose) isomerase from Actinoplanes missouriensis. 1. Crystallography and site-directed mutagenesis of metal binding sites."
      Jenkins J., Janin J., Rey F., Chiadmi M., van Tilbeurgh H., Lasters I., de Maeyer M., van Belle D., Wodak S.J., Lauwereys M., Stanssens P., Mrabet N.T., Snauwaert J., Matthyssens G., Lambeir A.-M.
      Biochemistry 31:5449-5458(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS).
    5. "Multiwavelength anomalous solvent contrast (MASC): derivation of envelope structure-factor amplitudes and comparison with model values."
      Ramin M., Shepard W., Fourme R., Kahn R.
      Acta Crystallogr. D 55:157-167(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (4.1 ANGSTROMS).
    6. "Protein engineering of xylose (glucose) isomerase from Actinoplanes missouriensis. 2. Site-directed mutagenesis of the xylose binding site."
      Lambeir A.-M., Lauwereys M., Stanssens P., Mrabet N.T., Snauwaert J., van Tilbeurgh H., Matthyssens G., Lasters I., de Maeyer M., Wodak S.J., Jenkins J., Chiadmi M., Janin J.
      Biochemistry 31:5459-5466(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS.
    7. "Protein engineering of xylose (glucose) isomerase from Actinoplanes missouriensis. 3. Changing metal specificity and the pH profile by site-directed mutagenesis."
      van Tilbeurgh H., Jenkins J., Chiadmi M., Janin J., Wodak S.J., Mrabet N.T., Lambeir A.-M.
      Biochemistry 31:5467-5472(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS.

    Entry informationi

    Entry nameiXYLA_ACTM4
    AccessioniPrimary (citable) accession number: P12851
    Secondary accession number(s): I0GZR8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1989
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 109 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3