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P12849 (KAP1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 124. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
cAMP-dependent protein kinase type I-beta regulatory subunit
Gene names
Name:Prkar1b
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length381 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells By similarity.

Subunit structure

The inactive holoenzyme is composed of two regulatory chains and two catalytic chains. Activation by cAMP releases the two active catalytic monomers and the regulatory dimer. Interacts with PRKX; regulates this cAMP-dependent protein kinase By similarity. Interacts with smAKAP; this interaction may target PRKAR1B to the plasma membrane By similarity.

Subcellular location

Cell membrane By similarity.

Tissue specificity

Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.

Post-translational modification

The pseudophosphorylation site binds to the substrate-binding region of the catalytic chain, resulting in the inhibition of its activity.

Sequence similarities

Belongs to the cAMP-dependent kinase regulatory chain family.

Contains 2 cyclic nucleotide-binding domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed
Chain2 – 381380cAMP-dependent protein kinase type I-beta regulatory subunit
PRO_0000205382

Regions

Nucleotide binding137 – 254118cAMP 1
Nucleotide binding255 – 381127cAMP 2
Region2 – 136135Dimerization and phosphorylation
Motif96 – 1005Pseudophosphorylation motif

Sites

Binding site2021cAMP 1
Binding site2111cAMP 1
Binding site3261cAMP 2
Binding site3351cAMP 2

Amino acid modifications

Modified residue21N-acetylalanine Probable
Modified residue211Nitrated tyrosine By similarity
Modified residue771Phosphoserine By similarity
Modified residue831Phosphoserine By similarity
Disulfide bond18Interchain (with C-39) By similarity
Disulfide bond39Interchain (with C-18) By similarity

Sequences

Sequence LengthMass (Da)Tools
P12849 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: 42287E553FA16023

FASTA38143,224
        10         20         30         40         50         60 
MASPSCFHSE DEDSLKGCEM YVQKHGIQQV LKECIVHLCV AKPDRPLRFL REHFEKLEKE 

        70         80         90        100        110        120 
ENRQILARQK SNSQCDSHDE EISPTPPNPV VKARRRRGGV SAEVYTEEDA VSYVRKVIPK 

       130        140        150        160        170        180 
DYKTMTALAK AISKNVLFSH LDDNERSDIF DAMFPVTHIG GETVIQQGNE GDNFYVIDQG 

       190        200        210        220        230        240 
EVDVYVNGEW VTNISEGGSF GELALIYGTP RAATVKAKTD LKLWGIDRDS YRRILMGSTL 

       250        260        270        280        290        300 
RKRKMYEEFL SKVSILESLE KWERLTVADA LEPVQFEDGE KIVVQGEPGD DFYIITEGTA 

       310        320        330        340        350        360 
SVLQRRSPNE EYVEVGRLGP SDYFGEIALL LNRPRAATVV ARGPLKCVKL DRPRFERVLG 

       370        380 
PCSEILKRNI QRYNSFISLT V 

« Hide

References

[1]"Genetic characterization of a brain-specific form of the type I regulatory subunit of cAMP-dependent protein kinase."
Clegg C.H., Cadd G.G., McKnight G.S.
Proc. Natl. Acad. Sci. U.S.A. 85:3703-3707(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M20473 mRNA. Translation: AAA39935.1.
CCDSCCDS19803.1.
PIROKMSR1. A30205.
RefSeqNP_001240819.1. NM_001253890.1.
NP_032949.3. NM_008923.3.
XP_006504723.1. XM_006504660.1.
XP_006504724.1. XM_006504661.1.
XP_006504725.1. XM_006504662.1.
XP_006504726.1. XM_006504663.1.
UniGeneMm.306163.

3D structure databases

ProteinModelPortalP12849.
SMRP12849. Positions 14-380.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP12849. 5 interactions.

PTM databases

PhosphoSiteP12849.

Proteomic databases

MaxQBP12849.
PaxDbP12849.
PRIDEP12849.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000026973; ENSMUSP00000026973; ENSMUSG00000025855.
ENSMUST00000110890; ENSMUSP00000106515; ENSMUSG00000025855.
GeneID19085.
KEGGmmu:19085.
UCSCuc009afy.2. mouse.

Organism-specific databases

CTD5575.
MGIMGI:97759. Prkar1b.

Phylogenomic databases

eggNOGCOG0664.
GeneTreeENSGT00530000062947.
HOGENOMHOG000196669.
HOVERGENHBG002025.
InParanoidP12849.
KOK04739.
OMACKQIMAR.
OrthoDBEOG72JWG6.
PhylomeDBP12849.
TreeFamTF314920.

Gene expression databases

ArrayExpressP12849.
BgeeP12849.
GenevestigatorP12849.

Family and domain databases

Gene3D2.60.120.10. 2 hits.
InterProIPR002373. cAMP/cGMP_kin.
IPR012198. cAMP_dep_PK_reg_su.
IPR003117. cAMP_dep_PK_reg_su_I/II_a/b.
IPR018490. cNMP-bd-like.
IPR018488. cNMP-bd_CS.
IPR000595. cNMP-bd_dom.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PfamPF00027. cNMP_binding. 2 hits.
PF02197. RIIa. 1 hit.
[Graphical view]
PIRSFPIRSF000548. PK_regulatory. 1 hit.
PRINTSPR00103. CAMPKINASE.
SMARTSM00100. cNMP. 2 hits.
SM00394. RIIa. 1 hit.
[Graphical view]
SUPFAMSSF47391. SSF47391. 1 hit.
SSF51206. SSF51206. 2 hits.
PROSITEPS00888. CNMP_BINDING_1. 2 hits.
PS00889. CNMP_BINDING_2. 2 hits.
PS50042. CNMP_BINDING_3. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio295626.
PROP12849.
SOURCESearch...

Entry information

Entry nameKAP1_MOUSE
AccessionPrimary (citable) accession number: P12849
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: January 23, 2007
Last modified: July 9, 2014
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot