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P12849

- KAP1_MOUSE

UniProt

P12849 - KAP1_MOUSE

Protein

cAMP-dependent protein kinase type I-beta regulatory subunit

Gene

Prkar1b

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 126 (01 Oct 2014)
      Sequence version 2 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei202 – 2021cAMP 1
    Binding sitei211 – 2111cAMP 1
    Binding sitei326 – 3261cAMP 2
    Binding sitei335 – 3351cAMP 2

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi137 – 254118cAMP 1Add
    BLAST
    Nucleotide bindingi255 – 381127cAMP 2Add
    BLAST

    GO - Molecular functioni

    1. cAMP binding Source: UniProtKB-KW
    2. cAMP-dependent protein kinase inhibitor activity Source: Ensembl
    3. cAMP-dependent protein kinase regulator activity Source: MGI

    GO - Biological processi

    1. cell proliferation Source: MGI
    2. learning or memory Source: MGI
    3. negative regulation of cAMP-dependent protein kinase activity Source: Ensembl
    4. organ morphogenesis Source: MGI
    5. protein phosphorylation Source: MGI

    Keywords - Ligandi

    cAMP, cAMP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_198649. Factors involved in megakaryocyte development and platelet production.
    REACT_203795. DARPP-32 events.
    REACT_213947. Regulation of water balance by renal Aquaporins.
    REACT_220108. PKA activation.
    REACT_220758. PKA activation in glucagon signalling.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    cAMP-dependent protein kinase type I-beta regulatory subunit
    Gene namesi
    Name:Prkar1b
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 5

    Organism-specific databases

    MGIiMGI:97759. Prkar1b.

    Subcellular locationi

    Cell membrane By similarity

    GO - Cellular componenti

    1. cAMP-dependent protein kinase complex Source: InterPro
    2. cytoplasm Source: MGI
    3. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed
    Chaini2 – 381380cAMP-dependent protein kinase type I-beta regulatory subunitPRO_0000205382Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanineCurated
    Disulfide bondi18 – 18Interchain (with C-39)By similarity
    Modified residuei21 – 211Nitrated tyrosineBy similarity
    Disulfide bondi39 – 39Interchain (with C-18)By similarity
    Modified residuei77 – 771PhosphoserineBy similarity
    Modified residuei83 – 831PhosphoserineBy similarity

    Post-translational modificationi

    The pseudophosphorylation site binds to the substrate-binding region of the catalytic chain, resulting in the inhibition of its activity.

    Keywords - PTMi

    Acetylation, Disulfide bond, Nitration, Phosphoprotein

    Proteomic databases

    MaxQBiP12849.
    PaxDbiP12849.
    PRIDEiP12849.

    PTM databases

    PhosphoSiteiP12849.

    Expressioni

    Tissue specificityi

    Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.

    Gene expression databases

    ArrayExpressiP12849.
    BgeeiP12849.
    GenevestigatoriP12849.

    Interactioni

    Subunit structurei

    The inactive holoenzyme is composed of two regulatory chains and two catalytic chains. Activation by cAMP releases the two active catalytic monomers and the regulatory dimer. Interacts with PRKX; regulates this cAMP-dependent protein kinase By similarity. Interacts with smAKAP; this interaction may target PRKAR1B to the plasma membrane By similarity.By similarity

    Protein-protein interaction databases

    IntActiP12849. 5 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliP12849.
    SMRiP12849. Positions 14-380.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni2 – 136135Dimerization and phosphorylationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi96 – 1005Pseudophosphorylation motif

    Sequence similaritiesi

    Contains 2 cyclic nucleotide-binding domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG0664.
    GeneTreeiENSGT00530000062947.
    HOGENOMiHOG000196669.
    HOVERGENiHBG002025.
    InParanoidiP12849.
    KOiK04739.
    OMAiCKQIMAR.
    OrthoDBiEOG72JWG6.
    PhylomeDBiP12849.
    TreeFamiTF314920.

    Family and domain databases

    Gene3Di2.60.120.10. 2 hits.
    InterProiIPR002373. cAMP/cGMP_kin.
    IPR012198. cAMP_dep_PK_reg_su.
    IPR003117. cAMP_dep_PK_reg_su_I/II_a/b.
    IPR018490. cNMP-bd-like.
    IPR018488. cNMP-bd_CS.
    IPR000595. cNMP-bd_dom.
    IPR014710. RmlC-like_jellyroll.
    [Graphical view]
    PfamiPF00027. cNMP_binding. 2 hits.
    PF02197. RIIa. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000548. PK_regulatory. 1 hit.
    PRINTSiPR00103. CAMPKINASE.
    SMARTiSM00100. cNMP. 2 hits.
    SM00394. RIIa. 1 hit.
    [Graphical view]
    SUPFAMiSSF47391. SSF47391. 1 hit.
    SSF51206. SSF51206. 2 hits.
    PROSITEiPS00888. CNMP_BINDING_1. 2 hits.
    PS00889. CNMP_BINDING_2. 2 hits.
    PS50042. CNMP_BINDING_3. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P12849-1 [UniParc]FASTAAdd to Basket

    « Hide

    MASPSCFHSE DEDSLKGCEM YVQKHGIQQV LKECIVHLCV AKPDRPLRFL    50
    REHFEKLEKE ENRQILARQK SNSQCDSHDE EISPTPPNPV VKARRRRGGV 100
    SAEVYTEEDA VSYVRKVIPK DYKTMTALAK AISKNVLFSH LDDNERSDIF 150
    DAMFPVTHIG GETVIQQGNE GDNFYVIDQG EVDVYVNGEW VTNISEGGSF 200
    GELALIYGTP RAATVKAKTD LKLWGIDRDS YRRILMGSTL RKRKMYEEFL 250
    SKVSILESLE KWERLTVADA LEPVQFEDGE KIVVQGEPGD DFYIITEGTA 300
    SVLQRRSPNE EYVEVGRLGP SDYFGEIALL LNRPRAATVV ARGPLKCVKL 350
    DRPRFERVLG PCSEILKRNI QRYNSFISLT V 381
    Length:381
    Mass (Da):43,224
    Last modified:January 23, 2007 - v2
    Checksum:i42287E553FA16023
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M20473 mRNA. Translation: AAA39935.1.
    CCDSiCCDS19803.1.
    PIRiA30205. OKMSR1.
    RefSeqiNP_001240819.1. NM_001253890.1.
    NP_032949.3. NM_008923.3.
    XP_006504723.1. XM_006504660.1.
    XP_006504724.1. XM_006504661.1.
    XP_006504725.1. XM_006504662.1.
    XP_006504726.1. XM_006504663.1.
    UniGeneiMm.306163.

    Genome annotation databases

    EnsembliENSMUST00000026973; ENSMUSP00000026973; ENSMUSG00000025855.
    ENSMUST00000110890; ENSMUSP00000106515; ENSMUSG00000025855.
    GeneIDi19085.
    KEGGimmu:19085.
    UCSCiuc009afy.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M20473 mRNA. Translation: AAA39935.1 .
    CCDSi CCDS19803.1.
    PIRi A30205. OKMSR1.
    RefSeqi NP_001240819.1. NM_001253890.1.
    NP_032949.3. NM_008923.3.
    XP_006504723.1. XM_006504660.1.
    XP_006504724.1. XM_006504661.1.
    XP_006504725.1. XM_006504662.1.
    XP_006504726.1. XM_006504663.1.
    UniGenei Mm.306163.

    3D structure databases

    ProteinModelPortali P12849.
    SMRi P12849. Positions 14-380.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P12849. 5 interactions.

    PTM databases

    PhosphoSitei P12849.

    Proteomic databases

    MaxQBi P12849.
    PaxDbi P12849.
    PRIDEi P12849.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000026973 ; ENSMUSP00000026973 ; ENSMUSG00000025855 .
    ENSMUST00000110890 ; ENSMUSP00000106515 ; ENSMUSG00000025855 .
    GeneIDi 19085.
    KEGGi mmu:19085.
    UCSCi uc009afy.2. mouse.

    Organism-specific databases

    CTDi 5575.
    MGIi MGI:97759. Prkar1b.

    Phylogenomic databases

    eggNOGi COG0664.
    GeneTreei ENSGT00530000062947.
    HOGENOMi HOG000196669.
    HOVERGENi HBG002025.
    InParanoidi P12849.
    KOi K04739.
    OMAi CKQIMAR.
    OrthoDBi EOG72JWG6.
    PhylomeDBi P12849.
    TreeFami TF314920.

    Enzyme and pathway databases

    Reactomei REACT_198649. Factors involved in megakaryocyte development and platelet production.
    REACT_203795. DARPP-32 events.
    REACT_213947. Regulation of water balance by renal Aquaporins.
    REACT_220108. PKA activation.
    REACT_220758. PKA activation in glucagon signalling.

    Miscellaneous databases

    NextBioi 295626.
    PROi P12849.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P12849.
    Bgeei P12849.
    Genevestigatori P12849.

    Family and domain databases

    Gene3Di 2.60.120.10. 2 hits.
    InterProi IPR002373. cAMP/cGMP_kin.
    IPR012198. cAMP_dep_PK_reg_su.
    IPR003117. cAMP_dep_PK_reg_su_I/II_a/b.
    IPR018490. cNMP-bd-like.
    IPR018488. cNMP-bd_CS.
    IPR000595. cNMP-bd_dom.
    IPR014710. RmlC-like_jellyroll.
    [Graphical view ]
    Pfami PF00027. cNMP_binding. 2 hits.
    PF02197. RIIa. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000548. PK_regulatory. 1 hit.
    PRINTSi PR00103. CAMPKINASE.
    SMARTi SM00100. cNMP. 2 hits.
    SM00394. RIIa. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47391. SSF47391. 1 hit.
    SSF51206. SSF51206. 2 hits.
    PROSITEi PS00888. CNMP_BINDING_1. 2 hits.
    PS00889. CNMP_BINDING_2. 2 hits.
    PS50042. CNMP_BINDING_3. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genetic characterization of a brain-specific form of the type I regulatory subunit of cAMP-dependent protein kinase."
      Clegg C.H., Cadd G.G., McKnight G.S.
      Proc. Natl. Acad. Sci. U.S.A. 85:3703-3707(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Brain.

    Entry informationi

    Entry nameiKAP1_MOUSE
    AccessioniPrimary (citable) accession number: P12849
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1989
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 126 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3