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Protein

cAMP-dependent protein kinase type I-beta regulatory subunit

Gene

Prkar1b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei202 – 2021cAMP 1
Binding sitei211 – 2111cAMP 1
Binding sitei326 – 3261cAMP 2
Binding sitei335 – 3351cAMP 2

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi137 – 254118cAMP 1Add
BLAST
Nucleotide bindingi255 – 381127cAMP 2Add
BLAST

GO - Molecular functioni

GO - Biological processi

  • cell proliferation Source: MGI
  • learning or memory Source: MGI
  • negative regulation of cAMP-dependent protein kinase activity Source: MGI
  • organ morphogenesis Source: MGI
  • protein phosphorylation Source: MGI
Complete GO annotation...

Keywords - Ligandi

cAMP, cAMP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_278699. Hedgehog 'off' state.
REACT_280010. Factors involved in megakaryocyte development and platelet production.
REACT_286837. Vasopressin regulates renal water homeostasis via Aquaporins.
REACT_295338. PKA activation in glucagon signalling.
REACT_310977. PKA activation.
REACT_325290. DARPP-32 events.

Names & Taxonomyi

Protein namesi
Recommended name:
cAMP-dependent protein kinase type I-beta regulatory subunit
Gene namesi
Name:Prkar1b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:97759. Prkar1b.

Subcellular locationi

GO - Cellular componenti

  • cAMP-dependent protein kinase complex Source: InterPro
  • cytoplasm Source: MGI
  • plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed
Chaini2 – 381380cAMP-dependent protein kinase type I-beta regulatory subunitPRO_0000205382Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineCurated
Disulfide bondi18 – 18Interchain (with C-39)By similarity
Modified residuei21 – 211Nitrated tyrosineBy similarity
Disulfide bondi39 – 39Interchain (with C-18)By similarity
Modified residuei77 – 771PhosphoserineBy similarity
Modified residuei83 – 831PhosphoserineBy similarity

Post-translational modificationi

The pseudophosphorylation site binds to the substrate-binding region of the catalytic chain, resulting in the inhibition of its activity.

Keywords - PTMi

Acetylation, Disulfide bond, Nitration, Phosphoprotein

Proteomic databases

MaxQBiP12849.
PaxDbiP12849.
PRIDEiP12849.

PTM databases

PhosphoSiteiP12849.

Expressioni

Tissue specificityi

Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.

Gene expression databases

BgeeiP12849.
ExpressionAtlasiP12849. baseline and differential.
GenevisibleiP12849. MM.

Interactioni

Subunit structurei

The inactive holoenzyme is composed of two regulatory chains and two catalytic chains. Activation by cAMP releases the two active catalytic monomers and the regulatory dimer. Interacts with PRKX; regulates this cAMP-dependent protein kinase (By similarity). Interacts with smAKAP; this interaction may target PRKAR1B to the plasma membrane (By similarity).By similarity

Protein-protein interaction databases

IntActiP12849. 5 interactions.
STRINGi10090.ENSMUSP00000026973.

Structurei

3D structure databases

ProteinModelPortaliP12849.
SMRiP12849. Positions 14-380.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2 – 136135Dimerization and phosphorylationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi96 – 1005Pseudophosphorylation motif

Sequence similaritiesi

Contains 2 cyclic nucleotide-binding domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG0664.
GeneTreeiENSGT00530000062947.
HOGENOMiHOG000196669.
HOVERGENiHBG002025.
InParanoidiP12849.
KOiK04739.
OMAiRAEYSAK.
OrthoDBiEOG72JWG6.
PhylomeDBiP12849.
TreeFamiTF314920.

Family and domain databases

Gene3Di2.60.120.10. 2 hits.
InterProiIPR002373. cAMP/cGMP_kin.
IPR012198. cAMP_dep_PK_reg_su.
IPR003117. cAMP_dep_PK_reg_su_I/II_a/b.
IPR018490. cNMP-bd-like.
IPR018488. cNMP-bd_CS.
IPR000595. cNMP-bd_dom.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PfamiPF00027. cNMP_binding. 2 hits.
PF02197. RIIa. 1 hit.
[Graphical view]
PIRSFiPIRSF000548. PK_regulatory. 1 hit.
PRINTSiPR00103. CAMPKINASE.
SMARTiSM00100. cNMP. 2 hits.
SM00394. RIIa. 1 hit.
[Graphical view]
SUPFAMiSSF47391. SSF47391. 1 hit.
SSF51206. SSF51206. 2 hits.
PROSITEiPS00888. CNMP_BINDING_1. 2 hits.
PS00889. CNMP_BINDING_2. 2 hits.
PS50042. CNMP_BINDING_3. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P12849-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASPSCFHSE DEDSLKGCEM YVQKHGIQQV LKECIVHLCV AKPDRPLRFL
60 70 80 90 100
REHFEKLEKE ENRQILARQK SNSQCDSHDE EISPTPPNPV VKARRRRGGV
110 120 130 140 150
SAEVYTEEDA VSYVRKVIPK DYKTMTALAK AISKNVLFSH LDDNERSDIF
160 170 180 190 200
DAMFPVTHIG GETVIQQGNE GDNFYVIDQG EVDVYVNGEW VTNISEGGSF
210 220 230 240 250
GELALIYGTP RAATVKAKTD LKLWGIDRDS YRRILMGSTL RKRKMYEEFL
260 270 280 290 300
SKVSILESLE KWERLTVADA LEPVQFEDGE KIVVQGEPGD DFYIITEGTA
310 320 330 340 350
SVLQRRSPNE EYVEVGRLGP SDYFGEIALL LNRPRAATVV ARGPLKCVKL
360 370 380
DRPRFERVLG PCSEILKRNI QRYNSFISLT V
Length:381
Mass (Da):43,224
Last modified:January 23, 2007 - v2
Checksum:i42287E553FA16023
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M20473 mRNA. Translation: AAA39935.1.
CCDSiCCDS19803.1.
PIRiA30205. OKMSR1.
RefSeqiNP_001240819.1. NM_001253890.1.
NP_032949.3. NM_008923.3.
XP_006504723.1. XM_006504660.2.
XP_006504724.1. XM_006504661.2.
XP_006504726.1. XM_006504663.1.
UniGeneiMm.306163.

Genome annotation databases

EnsembliENSMUST00000026973; ENSMUSP00000026973; ENSMUSG00000025855.
ENSMUST00000110890; ENSMUSP00000106515; ENSMUSG00000025855.
GeneIDi19085.
KEGGimmu:19085.
UCSCiuc009afy.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M20473 mRNA. Translation: AAA39935.1.
CCDSiCCDS19803.1.
PIRiA30205. OKMSR1.
RefSeqiNP_001240819.1. NM_001253890.1.
NP_032949.3. NM_008923.3.
XP_006504723.1. XM_006504660.2.
XP_006504724.1. XM_006504661.2.
XP_006504726.1. XM_006504663.1.
UniGeneiMm.306163.

3D structure databases

ProteinModelPortaliP12849.
SMRiP12849. Positions 14-380.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP12849. 5 interactions.
STRINGi10090.ENSMUSP00000026973.

PTM databases

PhosphoSiteiP12849.

Proteomic databases

MaxQBiP12849.
PaxDbiP12849.
PRIDEiP12849.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000026973; ENSMUSP00000026973; ENSMUSG00000025855.
ENSMUST00000110890; ENSMUSP00000106515; ENSMUSG00000025855.
GeneIDi19085.
KEGGimmu:19085.
UCSCiuc009afy.2. mouse.

Organism-specific databases

CTDi5575.
MGIiMGI:97759. Prkar1b.

Phylogenomic databases

eggNOGiCOG0664.
GeneTreeiENSGT00530000062947.
HOGENOMiHOG000196669.
HOVERGENiHBG002025.
InParanoidiP12849.
KOiK04739.
OMAiRAEYSAK.
OrthoDBiEOG72JWG6.
PhylomeDBiP12849.
TreeFamiTF314920.

Enzyme and pathway databases

ReactomeiREACT_278699. Hedgehog 'off' state.
REACT_280010. Factors involved in megakaryocyte development and platelet production.
REACT_286837. Vasopressin regulates renal water homeostasis via Aquaporins.
REACT_295338. PKA activation in glucagon signalling.
REACT_310977. PKA activation.
REACT_325290. DARPP-32 events.

Miscellaneous databases

ChiTaRSiPrkar1b. mouse.
NextBioi295626.
PROiP12849.
SOURCEiSearch...

Gene expression databases

BgeeiP12849.
ExpressionAtlasiP12849. baseline and differential.
GenevisibleiP12849. MM.

Family and domain databases

Gene3Di2.60.120.10. 2 hits.
InterProiIPR002373. cAMP/cGMP_kin.
IPR012198. cAMP_dep_PK_reg_su.
IPR003117. cAMP_dep_PK_reg_su_I/II_a/b.
IPR018490. cNMP-bd-like.
IPR018488. cNMP-bd_CS.
IPR000595. cNMP-bd_dom.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PfamiPF00027. cNMP_binding. 2 hits.
PF02197. RIIa. 1 hit.
[Graphical view]
PIRSFiPIRSF000548. PK_regulatory. 1 hit.
PRINTSiPR00103. CAMPKINASE.
SMARTiSM00100. cNMP. 2 hits.
SM00394. RIIa. 1 hit.
[Graphical view]
SUPFAMiSSF47391. SSF47391. 1 hit.
SSF51206. SSF51206. 2 hits.
PROSITEiPS00888. CNMP_BINDING_1. 2 hits.
PS00889. CNMP_BINDING_2. 2 hits.
PS50042. CNMP_BINDING_3. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genetic characterization of a brain-specific form of the type I regulatory subunit of cAMP-dependent protein kinase."
    Clegg C.H., Cadd G.G., McKnight G.S.
    Proc. Natl. Acad. Sci. U.S.A. 85:3703-3707(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.

Entry informationi

Entry nameiKAP1_MOUSE
AccessioniPrimary (citable) accession number: P12849
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: January 23, 2007
Last modified: July 22, 2015
This is version 134 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.