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Protein

Myosin-3

Gene

Myh3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Muscle contraction.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi179 – 1868ATPSequence analysis

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Motor protein, Muscle protein, Myosin

Keywords - Ligandi

Actin-binding, ATP-binding, Calmodulin-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Myosin-3
Alternative name(s):
Myosin heavy chain 3
Myosin heavy chain, fast skeletal muscle, embryonic
Gene namesi
Name:Myh3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi3138. Myh3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Thick filament

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 19401940Myosin-3PRO_0000123396Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei130 – 1301N6,N6,N6-trimethyllysineSequence analysis

Keywords - PTMi

Methylation

Proteomic databases

PaxDbiP12847.
PRIDEiP12847.

PTM databases

iPTMnetiP12847.

Interactioni

Subunit structurei

Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2).

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000004147.

Structurei

3D structure databases

ProteinModelPortaliP12847.
SMRiP12847. Positions 839-964.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini86 – 779694Myosin motorAdd
BLAST
Domaini782 – 81130IQPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni656 – 67823Actin-bindingAdd
BLAST
Regioni758 – 77215Actin-bindingAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili840 – 19331094Sequence analysisAdd
BLAST

Domaini

The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
Limited proteolysis of myosin heavy chain produces 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). HMM can be further cleaved into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).Curated

Sequence similaritiesi

Contains 1 IQ domain.PROSITE-ProRule annotation
Contains 1 myosin motor domain.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0161. Eukaryota.
COG5022. LUCA.
HOGENOMiHOG000173959.
HOVERGENiHBG004704.
InParanoidiP12847.
KOiK10352.
PhylomeDBiP12847.

Family and domain databases

Gene3Di4.10.270.10. 1 hit.
InterProiIPR000048. IQ_motif_EF-hand-BS.
IPR027401. Myosin-like_IQ_dom.
IPR001609. Myosin_head_motor_dom.
IPR004009. Myosin_N.
IPR002928. Myosin_tail.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00063. Myosin_head. 1 hit.
PF02736. Myosin_N. 1 hit.
PF01576. Myosin_tail_1. 1 hit.
[Graphical view]
PRINTSiPR00193. MYOSINHEAVY.
SMARTiSM00015. IQ. 2 hits.
SM00242. MYSc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS50096. IQ. 1 hit.
PS51456. MYOSIN_MOTOR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P12847-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSDTEMEVF GIAAPFLRKS EKERIEAQNQ PFDAKTYCFV VDSKEEYAKG
60 70 80 90 100
KIKSSQDGKV TVETEDNRTL VVKPEDVYAM NPPKFDKIED MAMLTHLNEP
110 120 130 140 150
AVLYNLKDRY TSWMIYTYSG LFCVTVNPYK WLPVYTPEVV DGYRGKKRQE
160 170 180 190 200
APPHIFSISD NAYQFMLTDR ENQSILITGE SGAGKTVNTK RVIQYFATIA
210 220 230 240 250
ATGDLAKKKD SKMKGTLEDQ IISANPLLEA FGNAKTVRND NSSRFGKFIR
260 270 280 290 300
IHFGTTGKLA SADIETYLLE KSRVTFQLKA ERSYHIFYQI LSNKKPELIE
310 320 330 340 350
LLLITTNPYD YPFISQGEIL VASIDDREEL LATDSAIDIL GFTPEEKSGL
360 370 380 390 400
YKLTGAVMHY GNMKFKQKQR EEQAEPDGTE VADKTAYLMG LNSSDLLKAL
410 420 430 440 450
CFPRVKVGNE YVTKGQTVDQ VHHAVNALSK SVYEKLFLWM VTRINQQLDT
460 470 480 490 500
KLPRQHFIGV LDIAGFEIFE YNSLEQLCIN FTNEKLQQFF NHHMFVLEQE
510 520 530 540 550
EYKKEGIEWT FIDFGMDLAA CIELIEKPMG IFSILEEECM FPKATDTSFK
560 570 580 590 600
NKLYDQHLGK SNNFQKPKVV KGKAEAHFSL IHYAGTVDYS VSGWLEKNKD
610 620 630 640 650
PLNETVVGLY QKSSNRLLAH LYATFATTDA DGGKKKVAKK KGSSFQTVSA
660 670 680 690 700
LFRENLNKLM SNLRTTHPHF VRCIIPNETK TPGAMEHSLV LHQLRCNGVL
710 720 730 740 750
EGIRICRKGF PNRILYGDFK QRYRVLNASA IPEGQFIDSK KACEKLLASI
760 770 780 790 800
DIDHTQYKFG HTKVFFKAGL LGTLEEMRDE RLAKLITRTQ AVCRGFLMRV
810 820 830 840 850
EFQKMMQRRE SIFCIQYNIR AFMNVKHWPW MKLFFKIKPL LKSAETEKEM
860 870 880 890 900
ATMKEEFQKT KDELAKSEAK RKELEEKLVT LVQEKNDLQL QVQAESENLL
910 920 930 940 950
DAEERCDQLI KAKFQLEAKI KEVTERAEDE EEINAELTAK KRKLEDECSE
960 970 980 990 1000
LKKDIDDLEL TLAKVEKEKH ATENKVKNLT EELAGLDETI AKLTREKKAL
1010 1020 1030 1040 1050
QEAHQQTLDD LQAEEDKVNS LSKLKSKLEQ QVDDLESSLE QEKKLRVDLE
1060 1070 1080 1090 1100
RNKRKLEGDL KLAQESILDL ENDKQQLDER LKKKDFEYSQ LQSKVEDEQT
1110 1120 1130 1140 1150
LSLQLQKKIK ELQARIEELE EEIEAERATR AKTEKQRSDY ARELEELSER
1160 1170 1180 1190 1200
LEEAGGVTST QIELNKKREA EFLKLRRDLE EATLQHEATV ATLRKKHADS
1210 1220 1230 1240 1250
AAELAEQIDN LQRVKQKLEK EKSEFKLEID DLSSSVESVS KSKANLEKIC
1260 1270 1280 1290 1300
RTLEDQLSEA RGKNEETQRS LSELTTQKSR LQTEAGELSR QLEEKESIVS
1310 1320 1330 1340 1350
QLSRSKQAFT QQIEELKRQL EEENKAKNAL AHALQSSRHD CDLLREQYEE
1360 1370 1380 1390 1400
EQEGKAELQR ALSKANSEVA QWRTKYETDA IQRTEELEEA KKKLAQRLQD
1410 1420 1430 1440 1450
SEEQVEAVNA KCASLEKTKQ RLQGEVEDLM VDVERANSLA AALDKKQRNF
1460 1470 1480 1490 1500
DKVLAEWKTK CEESQAELEA ALKESRSLST ELFKLKNAYE EALDQLETVK
1510 1520 1530 1540 1550
RENKNLEQEI ADLTEQIAEN GKSIHELEKS RKQMELEKAD IQMALEEAEA
1560 1570 1580 1590 1600
ALEHEEAKIL RIQLELTQVK SEIDRKIAEK DEEIEQLKRN YQRTVETMQG
1610 1620 1630 1640 1650
ALDAEVRSRN EAIRLKKKME GDLNEIEIQL SHANRQAAET IKHLRSVQGQ
1660 1670 1680 1690 1700
LKDTQLHLDD ALRGQEDLKE QLAIVERRAN LLQAEVEELR ATLEQTERAR
1710 1720 1730 1740 1750
KLAEQELLDS NERVQLLHTQ NTSLIHTKKK LETDLTQLQS EVEDASRDAR
1760 1770 1780 1790 1800
NAEEKAKKAI TDAAMMAEEL KKEQDTSAHL ERMKKNLEQT VKDLQHRLDE
1810 1820 1830 1840 1850
AEQLALKGGK KQIQKLETRI RELEFELEGE QKRNTESVKG LRKYERRVKE
1860 1870 1880 1890 1900
LTYQSEEDRK NVLRLQDLVD KLQVKVKSYK RQAEEADEQA NVHLTKFRKA
1910 1920 1930 1940
QHELEEAEER ADIAESQVNK LRAKTRDFTS SRMVVHESEE
Length:1,940
Mass (Da):223,858
Last modified:October 1, 1989 - v1
Checksum:iB5D546A596E5A696
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04267 Genomic DNA. Translation: CAA27817.1.
PIRiA24922.
RefSeqiNP_036736.1. NM_012604.1.
UniGeneiRn.98847.

Genome annotation databases

GeneIDi24583.
KEGGirno:24583.
UCSCiRGD:3138. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04267 Genomic DNA. Translation: CAA27817.1.
PIRiA24922.
RefSeqiNP_036736.1. NM_012604.1.
UniGeneiRn.98847.

3D structure databases

ProteinModelPortaliP12847.
SMRiP12847. Positions 839-964.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000004147.

PTM databases

iPTMnetiP12847.

Proteomic databases

PaxDbiP12847.
PRIDEiP12847.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi24583.
KEGGirno:24583.
UCSCiRGD:3138. rat.

Organism-specific databases

CTDi4621.
RGDi3138. Myh3.

Phylogenomic databases

eggNOGiKOG0161. Eukaryota.
COG5022. LUCA.
HOGENOMiHOG000173959.
HOVERGENiHBG004704.
InParanoidiP12847.
KOiK10352.
PhylomeDBiP12847.

Miscellaneous databases

PROiP12847.

Family and domain databases

Gene3Di4.10.270.10. 1 hit.
InterProiIPR000048. IQ_motif_EF-hand-BS.
IPR027401. Myosin-like_IQ_dom.
IPR001609. Myosin_head_motor_dom.
IPR004009. Myosin_N.
IPR002928. Myosin_tail.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00063. Myosin_head. 1 hit.
PF02736. Myosin_N. 1 hit.
PF01576. Myosin_tail_1. 1 hit.
[Graphical view]
PRINTSiPR00193. MYOSINHEAVY.
SMARTiSM00015. IQ. 2 hits.
SM00242. MYSc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS50096. IQ. 1 hit.
PS51456. MYOSIN_MOTOR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete nucleotide and encoded amino acid sequence of a mammalian myosin heavy chain gene. Evidence against intron-dependent evolution of the rod."
    Strehler E.E., Strehler-Page M.-A., Perriard J.-C., Periasamy M., Nadal-Ginard B.
    J. Mol. Biol. 190:291-317(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Intron positions are conserved in the 5' end region of myosin heavy-chain genes."
    Strehler E.E., Mahdavi V., Periasamy M., Nadal-Ginard B.
    J. Biol. Chem. 260:468-471(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-168.
  3. "Characterization of cDNA and genomic sequences corresponding to an embryonic myosin heavy chain."
    Periasamy M., Wydro R.M., Strehler-Page M.-A., Strehler E.E., Nadal-Ginard B.
    J. Biol. Chem. 260:15856-15862(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1453-1940.

Entry informationi

Entry nameiMYH3_RAT
AccessioniPrimary (citable) accession number: P12847
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 1, 1989
Last modified: June 8, 2016
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

Represents a conventional myosin. This protein should not be confused with the unconventional myosin-3 (MYO3).Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.