Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Insulin-like growth factor-binding protein 2

Gene

Igfbp2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Inhibits IGF-mediated growth and developmental rates (By similarity). IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.By similarity

GO - Molecular functioni

  1. insulin-like growth factor I binding Source: UniProtKB
  2. insulin-like growth factor II binding Source: UniProtKB

GO - Biological processi

  1. aging Source: RGD
  2. cellular response to hormone stimulus Source: RGD
  3. female pregnancy Source: RGD
  4. multicellular organismal development Source: UniProtKB-KW
  5. positive regulation of activated T cell proliferation Source: UniProtKB
  6. regulation of cell growth Source: InterPro
  7. regulation of insulin-like growth factor receptor signaling pathway Source: UniProtKB
  8. response to drug Source: RGD
  9. response to estradiol Source: RGD
  10. response to estrogen Source: RGD
  11. response to glucocorticoid Source: RGD
  12. response to lithium ion Source: RGD
  13. response to mechanical stimulus Source: RGD
  14. response to nutrient Source: RGD
  15. response to retinoic acid Source: RGD
  16. response to steroid hormone Source: RGD
  17. signal transduction Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Growth regulation

Keywords - Ligandi

Growth factor binding

Enzyme and pathway databases

ReactomeiREACT_277593. Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs).

Protein family/group databases

MEROPSiI31.953.

Names & Taxonomyi

Protein namesi
Recommended name:
Insulin-like growth factor-binding protein 2
Short name:
IBP-2
Short name:
IGF-binding protein 2
Short name:
IGFBP-2
Alternative name(s):
BRL-BP
Gene namesi
Name:Igfbp2
Synonyms:Igfbp-2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 9

Organism-specific databases

RGDi2873. Igfbp2.

Subcellular locationi

Secreted 3 Publications

GO - Cellular componenti

  1. apical plasma membrane Source: RGD
  2. cytoplasmic vesicle Source: RGD
  3. extracellular region Source: UniProtKB
  4. extracellular space Source: RGD
  5. extracellular vesicular exosome Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 34341 PublicationAdd
BLAST
Chaini35 – 304270Insulin-like growth factor-binding protein 2PRO_0000014373Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi206 ↔ 240PROSITE-ProRule annotation
Disulfide bondi251 ↔ 262PROSITE-ProRule annotation
Disulfide bondi264 ↔ 285PROSITE-ProRule annotation

Post-translational modificationi

O-glycosylated.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiP12843.

PTM databases

PhosphoSiteiP12843.

Expressioni

Tissue specificityi

In adults, expressed in brain, testes, ovaries, and kidney. Expression in the adult liver is barely detectable.1 Publication

Developmental stagei

Predominantly expressed at fetal stages with highest expression in fetal liver. Also expressed in fetal kidney, intestine and lung, as well as muscle, heart and stomach.2 Publications

Gene expression databases

ExpressionAtlasiP12843. baseline and differential.
GenevestigatoriP12843.

Interactioni

Subunit structurei

Binds IGF2 more than IGF1.

Structurei

3D structure databases

ProteinModelPortaliP12843.
SMRiP12843. Positions 199-304.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini36 – 11883IGFBP N-terminalPROSITE-ProRule annotationAdd
BLAST
Domaini203 – 28583Thyroglobulin type-1PROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi280 – 2823Cell attachment site

Domaini

The C-terminus is required for IGF-binding and growth inhibition.1 Publication

Sequence similaritiesi

Contains 1 IGFBP N-terminal domain.PROSITE-ProRule annotation
Contains 1 thyroglobulin type-1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG47500.
GeneTreeiENSGT00550000074457.
HOGENOMiHOG000253012.
HOVERGENiHBG002631.
InParanoidiP12843.
OMAiGAGTCEK.
OrthoDBiEOG74N5HG.
PhylomeDBiP12843.
TreeFamiTF331211.

Family and domain databases

Gene3Di4.10.40.20. 1 hit.
4.10.800.10. 1 hit.
InterProiIPR009030. Growth_fac_rcpt_N_dom.
IPR012210. IGFBP-2.
IPR000867. IGFBP-like.
IPR009168. IGFBP1-6.
IPR022321. IGFBP_1-6_chordata.
IPR017891. Insulin_GF-bd_Cys-rich_CS.
IPR000716. Thyroglobulin_1.
[Graphical view]
PANTHERiPTHR11551. PTHR11551. 1 hit.
PTHR11551:SF5. PTHR11551:SF5. 1 hit.
PfamiPF00219. IGFBP. 1 hit.
PF00086. Thyroglobulin_1. 1 hit.
[Graphical view]
PRINTSiPR01976. IGFBPFAMILY.
PR01978. IGFBPFAMILY2.
SMARTiSM00121. IB. 1 hit.
SM00211. TY. 1 hit.
[Graphical view]
SUPFAMiSSF57184. SSF57184. 1 hit.
SSF57610. SSF57610. 1 hit.
PROSITEiPS00222. IGFBP_N_1. 1 hit.
PS51323. IGFBP_N_2. 1 hit.
PS00484. THYROGLOBULIN_1_1. 1 hit.
PS51162. THYROGLOBULIN_1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P12843-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLPRLGGPAL PLLLPSLLLL LLLGAGGCGP GVRAEVLFRC PPCTPERLAA
60 70 80 90 100
CGPPPDAPCA ELVREPGCGC CSVCARQEGE ACGVYIPRCA QTLRCYPNPG
110 120 130 140 150
SELPLKALVT GAGTCEKRRV GATPQQVADS EDDHSEGGLV ENHVDGTMNM
160 170 180 190 200
LGGSSAGRKP PKSGMKELAV FREKVNEQHR QMGKGAKHLS LEEPKKLRPP
210 220 230 240 250
PARTPCQQEL DQVLERISTM RLPDDRGPLE HLYSLHIPNC DKHGLYNLKQ
260 270 280 290 300
CKMSLNGQRG ECWCVNPNTG KPIQGAPTIR GDPECHLFYN EQQENDGAHA

QRVQ
Length:304
Mass (Da):32,855
Last modified:April 30, 2007 - v3
Checksum:i8558B7E2C9152B9F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti298 – 2981A → V in AAA40829 (PubMed:2538475).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04486 mRNA. Translation: AAA40829.1.
M31672 mRNA. Translation: AAA41381.1.
BC092570 mRNA. Translation: AAH92570.1.
PIRiA33274.
RefSeqiNP_037254.2. NM_013122.2.
UniGeneiRn.6813.

Genome annotation databases

EnsembliENSRNOT00000023068; ENSRNOP00000023068; ENSRNOG00000016957.
GeneIDi25662.
KEGGirno:25662.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04486 mRNA. Translation: AAA40829.1.
M31672 mRNA. Translation: AAA41381.1.
BC092570 mRNA. Translation: AAH92570.1.
PIRiA33274.
RefSeqiNP_037254.2. NM_013122.2.
UniGeneiRn.6813.

3D structure databases

ProteinModelPortaliP12843.
SMRiP12843. Positions 199-304.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiI31.953.

PTM databases

PhosphoSiteiP12843.

Proteomic databases

PRIDEiP12843.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000023068; ENSRNOP00000023068; ENSRNOG00000016957.
GeneIDi25662.
KEGGirno:25662.

Organism-specific databases

CTDi3485.
RGDi2873. Igfbp2.

Phylogenomic databases

eggNOGiNOG47500.
GeneTreeiENSGT00550000074457.
HOGENOMiHOG000253012.
HOVERGENiHBG002631.
InParanoidiP12843.
OMAiGAGTCEK.
OrthoDBiEOG74N5HG.
PhylomeDBiP12843.
TreeFamiTF331211.

Enzyme and pathway databases

ReactomeiREACT_277593. Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs).

Miscellaneous databases

NextBioi607569.
PROiP12843.

Gene expression databases

ExpressionAtlasiP12843. baseline and differential.
GenevestigatoriP12843.

Family and domain databases

Gene3Di4.10.40.20. 1 hit.
4.10.800.10. 1 hit.
InterProiIPR009030. Growth_fac_rcpt_N_dom.
IPR012210. IGFBP-2.
IPR000867. IGFBP-like.
IPR009168. IGFBP1-6.
IPR022321. IGFBP_1-6_chordata.
IPR017891. Insulin_GF-bd_Cys-rich_CS.
IPR000716. Thyroglobulin_1.
[Graphical view]
PANTHERiPTHR11551. PTHR11551. 1 hit.
PTHR11551:SF5. PTHR11551:SF5. 1 hit.
PfamiPF00219. IGFBP. 1 hit.
PF00086. Thyroglobulin_1. 1 hit.
[Graphical view]
PRINTSiPR01976. IGFBPFAMILY.
PR01978. IGFBPFAMILY2.
SMARTiSM00121. IB. 1 hit.
SM00211. TY. 1 hit.
[Graphical view]
SUPFAMiSSF57184. SSF57184. 1 hit.
SSF57610. SSF57610. 1 hit.
PROSITEiPS00222. IGFBP_N_1. 1 hit.
PS51323. IGFBP_N_2. 1 hit.
PS00484. THYROGLOBULIN_1_1. 1 hit.
PS51162. THYROGLOBULIN_1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence and expression of a cDNA clone encoding a fetal rat binding protein for insulin-like growth factors."
    Brown A.L., Chiariotti L., Orlowski C.C., Mehlman T., Burgers W.H., Ackerman E.J., Bruni C.B., Rechler M.M.
    J. Biol. Chem. 264:5148-5154(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, INTERACTION WITH IGF1 AND IGF2, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.
  2. "A low molecular weight insulin-like growth factor binding protein from rat: cDNA cloning and tissue distribution of its messenger RNA."
    Margot J.B., Binkert C., Mary J.-L., Landwehr J., Heinrich G., Schwander J.
    Mol. Endocrinol. 3:1053-1060(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Tissue: Liver.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Ovary.
  4. "Identification of a novel binding protein for insulin-like growth factors in adult rat serum."
    Shimonaka M., Schroeder R., Shimasaki S., Ling N.
    Biochem. Biophys. Res. Commun. 165:189-195(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 35-64, SUBCELLULAR LOCATION.
    Tissue: Serum.
  5. "Purification and amino-terminal sequence of an insulin-like growth factor-binding protein secreted by rat liver BRL-3A cells."
    Mottola C., Macdonald R.G., Brackett J.L., Mole J.E., Anderson J.K., Czech M.P.
    J. Biol. Chem. 261:11180-11188(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 38-68, SUBCELLULAR LOCATION.
  6. "Isolation of a biologically active fragment from the carboxy terminus of the fetal rat binding protein for insulin-like growth factors."
    Wang J.F., Hampton B., Mehlman T., Burgess W.H., Rechler M.M.
    Biochem. Biophys. Res. Commun. 157:718-726(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 178-204, INTERACTION WITH IGF2, DOMAIN.

Entry informationi

Entry nameiIBP2_RAT
AccessioniPrimary (citable) accession number: P12843
Secondary accession number(s): Q569C7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 30, 1989
Last sequence update: April 30, 2007
Last modified: March 31, 2015
This is version 126 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.