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P12843

- IBP2_RAT

UniProt

P12843 - IBP2_RAT

Protein

Insulin-like growth factor-binding protein 2

Gene

Igfbp2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 122 (01 Oct 2014)
      Sequence version 3 (01 May 2007)
      Previous versions | rss
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    Functioni

    Inhibits IGF-mediated growth and developmental rates By similarity. IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.By similarity

    GO - Molecular functioni

    1. insulin-like growth factor I binding Source: UniProtKB
    2. insulin-like growth factor II binding Source: UniProtKB

    GO - Biological processi

    1. aging Source: RGD
    2. cellular response to hormone stimulus Source: RGD
    3. female pregnancy Source: RGD
    4. multicellular organismal development Source: UniProtKB-KW
    5. positive regulation of activated T cell proliferation Source: UniProtKB
    6. regulation of cell growth Source: InterPro
    7. regulation of insulin-like growth factor receptor signaling pathway Source: UniProtKB
    8. response to drug Source: RGD
    9. response to estradiol Source: RGD
    10. response to estrogen Source: RGD
    11. response to glucocorticoid Source: RGD
    12. response to lithium ion Source: RGD
    13. response to mechanical stimulus Source: RGD
    14. response to nutrient Source: RGD
    15. response to retinoic acid Source: RGD
    16. response to steroid hormone Source: RGD
    17. signal transduction Source: RGD

    Keywords - Molecular functioni

    Developmental protein

    Keywords - Biological processi

    Growth regulation

    Keywords - Ligandi

    Growth factor binding

    Protein family/group databases

    MEROPSiI31.953.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Insulin-like growth factor-binding protein 2
    Short name:
    IBP-2
    Short name:
    IGF-binding protein 2
    Short name:
    IGFBP-2
    Alternative name(s):
    BRL-BP
    Gene namesi
    Name:Igfbp2
    Synonyms:Igfbp-2
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 9

    Organism-specific databases

    RGDi2873. Igfbp2.

    Subcellular locationi

    Secreted 3 Publications

    GO - Cellular componenti

    1. apical plasma membrane Source: RGD
    2. cytoplasmic vesicle Source: RGD
    3. extracellular region Source: UniProtKB
    4. extracellular space Source: RGD

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 34341 PublicationAdd
    BLAST
    Chaini35 – 304270Insulin-like growth factor-binding protein 2PRO_0000014373Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi206 ↔ 240PROSITE-ProRule annotation
    Disulfide bondi251 ↔ 262PROSITE-ProRule annotation
    Disulfide bondi264 ↔ 285PROSITE-ProRule annotation

    Post-translational modificationi

    O-glycosylated.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PRIDEiP12843.

    PTM databases

    PhosphoSiteiP12843.

    Expressioni

    Tissue specificityi

    In adults, expressed in brain, testes, ovaries, and kidney. Expression in the adult liver is barely detectable.1 Publication

    Developmental stagei

    Predominantly expressed at fetal stages with highest expression in fetal liver. Also expressed in fetal kidney, intestine and lung, as well as muscle, heart and stomach.2 Publications

    Gene expression databases

    ArrayExpressiP12843.
    GenevestigatoriP12843.

    Interactioni

    Subunit structurei

    Binds IGF2 more than IGF1.

    Structurei

    3D structure databases

    ProteinModelPortaliP12843.
    SMRiP12843. Positions 199-304.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini36 – 11883IGFBP N-terminalPROSITE-ProRule annotationAdd
    BLAST
    Domaini203 – 28583Thyroglobulin type-1PROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi280 – 2823Cell attachment site

    Domaini

    The C-terminus is required for IGF-binding and growth inhibition.1 Publication

    Sequence similaritiesi

    Contains 1 IGFBP N-terminal domain.PROSITE-ProRule annotation
    Contains 1 thyroglobulin type-1 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG47500.
    GeneTreeiENSGT00550000074457.
    HOGENOMiHOG000253012.
    HOVERGENiHBG002631.
    InParanoidiP12843.
    OMAiGAGTCEK.
    OrthoDBiEOG74N5HG.
    PhylomeDBiP12843.
    TreeFamiTF331211.

    Family and domain databases

    Gene3Di4.10.40.20. 1 hit.
    4.10.800.10. 1 hit.
    InterProiIPR009030. Growth_fac_rcpt_N_dom.
    IPR012210. IGFBP-2.
    IPR000867. IGFBP-like.
    IPR009168. IGFBP1-6.
    IPR022321. IGFBP_1-6_chordata.
    IPR017891. Insulin_GF-bd_Cys-rich_CS.
    IPR000716. Thyroglobulin_1.
    [Graphical view]
    PANTHERiPTHR11551. PTHR11551. 1 hit.
    PTHR11551:SF5. PTHR11551:SF5. 1 hit.
    PfamiPF00219. IGFBP. 1 hit.
    PF00086. Thyroglobulin_1. 1 hit.
    [Graphical view]
    PRINTSiPR01976. IGFBPFAMILY.
    PR01978. IGFBPFAMILY2.
    SMARTiSM00121. IB. 1 hit.
    SM00211. TY. 1 hit.
    [Graphical view]
    SUPFAMiSSF57184. SSF57184. 1 hit.
    SSF57610. SSF57610. 1 hit.
    PROSITEiPS00222. IGFBP_N_1. 1 hit.
    PS51323. IGFBP_N_2. 1 hit.
    PS00484. THYROGLOBULIN_1_1. 1 hit.
    PS51162. THYROGLOBULIN_1_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P12843-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLPRLGGPAL PLLLPSLLLL LLLGAGGCGP GVRAEVLFRC PPCTPERLAA    50
    CGPPPDAPCA ELVREPGCGC CSVCARQEGE ACGVYIPRCA QTLRCYPNPG 100
    SELPLKALVT GAGTCEKRRV GATPQQVADS EDDHSEGGLV ENHVDGTMNM 150
    LGGSSAGRKP PKSGMKELAV FREKVNEQHR QMGKGAKHLS LEEPKKLRPP 200
    PARTPCQQEL DQVLERISTM RLPDDRGPLE HLYSLHIPNC DKHGLYNLKQ 250
    CKMSLNGQRG ECWCVNPNTG KPIQGAPTIR GDPECHLFYN EQQENDGAHA 300
    QRVQ 304
    Length:304
    Mass (Da):32,855
    Last modified:May 1, 2007 - v3
    Checksum:i8558B7E2C9152B9F
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti298 – 2981A → V in AAA40829. (PubMed:2538475)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J04486 mRNA. Translation: AAA40829.1.
    M31672 mRNA. Translation: AAA41381.1.
    BC092570 mRNA. Translation: AAH92570.1.
    PIRiA33274.
    RefSeqiNP_037254.2. NM_013122.2.
    UniGeneiRn.6813.

    Genome annotation databases

    EnsembliENSRNOT00000023068; ENSRNOP00000023068; ENSRNOG00000016957.
    GeneIDi25662.
    KEGGirno:25662.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J04486 mRNA. Translation: AAA40829.1 .
    M31672 mRNA. Translation: AAA41381.1 .
    BC092570 mRNA. Translation: AAH92570.1 .
    PIRi A33274.
    RefSeqi NP_037254.2. NM_013122.2.
    UniGenei Rn.6813.

    3D structure databases

    ProteinModelPortali P12843.
    SMRi P12843. Positions 199-304.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi I31.953.

    PTM databases

    PhosphoSitei P12843.

    Proteomic databases

    PRIDEi P12843.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000023068 ; ENSRNOP00000023068 ; ENSRNOG00000016957 .
    GeneIDi 25662.
    KEGGi rno:25662.

    Organism-specific databases

    CTDi 3485.
    RGDi 2873. Igfbp2.

    Phylogenomic databases

    eggNOGi NOG47500.
    GeneTreei ENSGT00550000074457.
    HOGENOMi HOG000253012.
    HOVERGENi HBG002631.
    InParanoidi P12843.
    OMAi GAGTCEK.
    OrthoDBi EOG74N5HG.
    PhylomeDBi P12843.
    TreeFami TF331211.

    Miscellaneous databases

    NextBioi 607569.
    PROi P12843.

    Gene expression databases

    ArrayExpressi P12843.
    Genevestigatori P12843.

    Family and domain databases

    Gene3Di 4.10.40.20. 1 hit.
    4.10.800.10. 1 hit.
    InterProi IPR009030. Growth_fac_rcpt_N_dom.
    IPR012210. IGFBP-2.
    IPR000867. IGFBP-like.
    IPR009168. IGFBP1-6.
    IPR022321. IGFBP_1-6_chordata.
    IPR017891. Insulin_GF-bd_Cys-rich_CS.
    IPR000716. Thyroglobulin_1.
    [Graphical view ]
    PANTHERi PTHR11551. PTHR11551. 1 hit.
    PTHR11551:SF5. PTHR11551:SF5. 1 hit.
    Pfami PF00219. IGFBP. 1 hit.
    PF00086. Thyroglobulin_1. 1 hit.
    [Graphical view ]
    PRINTSi PR01976. IGFBPFAMILY.
    PR01978. IGFBPFAMILY2.
    SMARTi SM00121. IB. 1 hit.
    SM00211. TY. 1 hit.
    [Graphical view ]
    SUPFAMi SSF57184. SSF57184. 1 hit.
    SSF57610. SSF57610. 1 hit.
    PROSITEi PS00222. IGFBP_N_1. 1 hit.
    PS51323. IGFBP_N_2. 1 hit.
    PS00484. THYROGLOBULIN_1_1. 1 hit.
    PS51162. THYROGLOBULIN_1_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence and expression of a cDNA clone encoding a fetal rat binding protein for insulin-like growth factors."
      Brown A.L., Chiariotti L., Orlowski C.C., Mehlman T., Burgers W.H., Ackerman E.J., Bruni C.B., Rechler M.M.
      J. Biol. Chem. 264:5148-5154(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, INTERACTION WITH IGF1 AND IGF2, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.
    2. "A low molecular weight insulin-like growth factor binding protein from rat: cDNA cloning and tissue distribution of its messenger RNA."
      Margot J.B., Binkert C., Mary J.-L., Landwehr J., Heinrich G., Schwander J.
      Mol. Endocrinol. 3:1053-1060(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
      Tissue: Liver.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Ovary.
    4. "Identification of a novel binding protein for insulin-like growth factors in adult rat serum."
      Shimonaka M., Schroeder R., Shimasaki S., Ling N.
      Biochem. Biophys. Res. Commun. 165:189-195(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 35-64, SUBCELLULAR LOCATION.
      Tissue: Serum.
    5. "Purification and amino-terminal sequence of an insulin-like growth factor-binding protein secreted by rat liver BRL-3A cells."
      Mottola C., Macdonald R.G., Brackett J.L., Mole J.E., Anderson J.K., Czech M.P.
      J. Biol. Chem. 261:11180-11188(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 38-68, SUBCELLULAR LOCATION.
    6. "Isolation of a biologically active fragment from the carboxy terminus of the fetal rat binding protein for insulin-like growth factors."
      Wang J.F., Hampton B., Mehlman T., Burgess W.H., Rechler M.M.
      Biochem. Biophys. Res. Commun. 157:718-726(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 178-204, INTERACTION WITH IGF2, DOMAIN.

    Entry informationi

    Entry nameiIBP2_RAT
    AccessioniPrimary (citable) accession number: P12843
    Secondary accession number(s): Q569C7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1989
    Last sequence update: May 1, 2007
    Last modified: October 1, 2014
    This is version 122 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3