P12843 (IBP2_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Insulin-like growth factor-binding protein 2
Short name=IBP-2
Short name=IGF-binding protein 2
Short name=IGFBP-2

Alternative name(s):
BRL-BP

Gene names

Name:	Igfbp2
Synonyms:	Igfbp-2

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	304 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Inhibits IGF-mediated growth and developmental rates By similarity. IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
Subunit structure	Binds IGF2 more than IGF1.
Subcellular location	Secreted Ref.1 Ref.4 Ref.5.
Tissue specificity	In adults, expressed in brain, testes, ovaries, and kidney. Expression in the adult liver is barely detectable. Ref.2
Developmental stage	Predominantly expressed at fetal stages with highest expression in fetal liver. Also expressed in fetal kidney, intestine and lung, as well as muscle, heart and stomach. Ref.1 Ref.2
Domain	The C-terminus is required for IGF-binding and growth inhibition. Ref.6
Post-translational modification	O-glycosylated By similarity.
Sequence similarities	Contains 1 IGFBP N-terminal domain. Contains 1 thyroglobulin type-1 domain.

Ontologies

Keywords
Biological process	Growth regulation
Cellular component	Secreted
Domain	Signal
Ligand	Growth factor binding
Molecular function	Developmental protein
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	aging Inferred from expression pattern PubMed 10191834. Source: RGD cellular response to hormone stimulus Inferred from expression pattern PubMed 16720626. Source: RGD female pregnancy Inferred from expression pattern PubMed 17123939. Source: RGD multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW positive regulation of activated T cell proliferation Inferred from sequence or structural similarity. Source: UniProtKB regulation of cell growth Inferred from electronic annotation. Source: InterPro regulation of insulin-like growth factor receptor signaling pathway Inferred from sequence or structural similarity. Source: UniProtKB response to drug Inferred from expression pattern PubMed 15613074. Source: RGD response to estradiol stimulus Inferred from expression pattern PubMed 10842239. Source: RGD response to glucocorticoid stimulus Inferred from expression pattern PubMed 15705658. Source: RGD response to lithium ion Inferred from expression pattern PubMed 16738484. Source: RGD response to mechanical stimulus Inferred from expression pattern PubMed 17308996. Source: RGD response to nutrient Inferred from expression pattern PubMed 15576465. Source: RGD response to retinoic acid Inferred from expression pattern PubMed 11880314. Source: RGD response to stress Inferred from expression pattern PubMed 16848927. Source: RGD signal transduction Inferred from expression pattern PubMed 11914028. Source: RGD
Cellular_component	apical plasma membrane Inferred from direct assay PubMed 10842239. Source: RGD cytoplasmic vesicle Inferred from direct assay PubMed 15705658. Source: RGD extracellular space Inferred from direct assay Ref.1PubMed 9396554. Source: RGD
Molecular_function	insulin-like growth factor I binding Inferred from direct assay Ref.1. Source: UniProtKB insulin-like growth factor II binding Inferred from direct assay PubMed 1709938Ref.1. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 34	34	Ref.4
	Chain	35 – 304	270	Insulin-like growth factor-binding protein 2		PRO_0000014373
Regions
	Domain	36 – 118	83	IGFBP N-terminal
	Domain	203 – 285	83	Thyroglobulin type-1
	Motif	280 – 282	3	Cell attachment site
Amino acid modifications
	Disulfide bond	206 ↔ 240		By similarity
	Disulfide bond	251 ↔ 262		By similarity
	Disulfide bond	264 ↔ 285		By similarity
Experimental info
	Sequence conflict	298	1	A → V in AAA40829. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P12843 [UniParc]. Last modified May 1, 2007. Version 3. Checksum: 8558B7E2C9152B9F	FASTA	304	32,855
	10 20 30 40 50 60 MLPRLGGPAL PLLLPSLLLL LLLGAGGCGP GVRAEVLFRC PPCTPERLAA CGPPPDAPCA 70 80 90 100 110 120 ELVREPGCGC CSVCARQEGE ACGVYIPRCA QTLRCYPNPG SELPLKALVT GAGTCEKRRV 130 140 150 160 170 180 GATPQQVADS EDDHSEGGLV ENHVDGTMNM LGGSSAGRKP PKSGMKELAV FREKVNEQHR 190 200 210 220 230 240 QMGKGAKHLS LEEPKKLRPP PARTPCQQEL DQVLERISTM RLPDDRGPLE HLYSLHIPNC 250 260 270 280 290 300 DKHGLYNLKQ CKMSLNGQRG ECWCVNPNTG KPIQGAPTIR GDPECHLFYN EQQENDGAHA QRVQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence and expression of a cDNA clone encoding a fetal rat binding protein for insulin-like growth factors." Brown A.L., Chiariotti L., Orlowski C.C., Mehlman T., Burgers W.H., Ackerman E.J., Bruni C.B., Rechler M.M. J. Biol. Chem. 264:5148-5154(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, INTERACTION WITH IGF1 AND IGF2, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.
[2]	"A low molecular weight insulin-like growth factor binding protein from rat: cDNA cloning and tissue distribution of its messenger RNA." Margot J.B., Binkert C., Mary J.-L., Landwehr J., Heinrich G., Schwander J. Mol. Endocrinol. 3:1053-1060(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE. Tissue: Liver.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Ovary.
[4]	"Identification of a novel binding protein for insulin-like growth factors in adult rat serum." Shimonaka M., Schroeder R., Shimasaki S., Ling N. Biochem. Biophys. Res. Commun. 165:189-195(1989) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 35-64, SUBCELLULAR LOCATION. Tissue: Serum.
[5]	"Purification and amino-terminal sequence of an insulin-like growth factor-binding protein secreted by rat liver BRL-3A cells." Mottola C., Macdonald R.G., Brackett J.L., Mole J.E., Anderson J.K., Czech M.P. J. Biol. Chem. 261:11180-11188(1986) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 38-68, SUBCELLULAR LOCATION.
[6]	"Isolation of a biologically active fragment from the carboxy terminus of the fetal rat binding protein for insulin-like growth factors." Wang J.F., Hampton B., Mehlman T., Burgess W.H., Rechler M.M. Biochem. Biophys. Res. Commun. 157:718-726(1988) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 178-204, INTERACTION WITH IGF2, DOMAIN.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	J04486 mRNA. Translation: AAA40829.1. M31672 mRNA. Translation: AAA41381.1. BC092570 mRNA. Translation: AAH92570.1.
IPI	IPI00201573.
PIR	A33274.
RefSeq	NP_037254.2. NM_013122.2.
UniGene	Rn.6813.
3D structure databases
ProteinModelPortal	P12843.
SMR	P12843. Positions 199-304.
ModBase	Search...
Protein family/group databases
MEROPS	I31.953.
PTM databases
PhosphoSite	P12843.
Proteomic databases
PRIDE	P12843.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000023068; ENSRNOP00000023068; ENSRNOG00000016957.
GeneID	25662.
KEGG	rno:25662.
Organism-specific databases
CTD	3485.
RGD	2873. Igfbp2.
Phylogenomic databases
eggNOG	NOG47500.
GeneTree	ENSGT00550000074457.
HOGENOM	HOG000253012.
HOVERGEN	HBG002631.
InParanoid	P12843.
OMA	GAGTCEK.
OrthoDB	EOG4TTGJD.
Gene expression databases
ArrayExpress	P12843.
Genevestigator	P12843.
GermOnline	ENSRNOG00000016957. Rattus norvegicus.
Family and domain databases
Gene3D	4.10.800.10. 1 hit.
InterPro	IPR009030. Growth_fac_rcpt. IPR012210. IGFBP-2. IPR000867. IGFBP-like. IPR009168. IGFBP1-6. IPR022321. IGFBP_1-6_chordata. IPR017891. Insulin_GF-bd_Cys-rich_CS. IPR000716. Thyroglobulin_1. [Graphical view]
PANTHER	PTHR11551. PTHR11551. 1 hit. PTHR11551:SF5. PTHR11551:SF5. 1 hit.
Pfam	PF00219. IGFBP. 1 hit. PF00086. Thyroglobulin_1. 1 hit. [Graphical view]
PRINTS	PR01976. IGFBPFAMILY. PR01978. IGFBPFAMILY2.
SMART	SM00121. IB. 1 hit. SM00211. TY. 1 hit. [Graphical view]
SUPFAM	SSF57184. Grow_fac_recept. 1 hit. SSF57610. Thyroglobulin_1. 1 hit.
PROSITE	PS00222. IGFBP_N_1. 1 hit. PS51323. IGFBP_N_2. 1 hit. PS00484. THYROGLOBULIN_1_1. 1 hit. PS51162. THYROGLOBULIN_1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	607569.

Entry information

Entry name

IBP2_RAT

Accession

Primary (citable) accession number: P12843
Secondary accession number(s): Q569C7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	May 1, 2007
Last modified:	April 3, 2013
This is version 113 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program