Neurofilament medium polypeptide - Rattus norvegicus (Rat)

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P12839 (NFM_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 115. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Neurofilament medium polypeptide
Short name=NF-M

Alternative name(s):
160 kDa neurofilament protein
Neurofilament 3
Neurofilament triplet M protein

Gene names

Name:	Nefm
Synonyms:	Nef3, Nfm

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	846 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function

Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber, AND MASS SPECTROMETRY.

Post-translational modification

There are a number of repeats of the tripeptide K-S-P, NFM is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFM results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber. Ref.4

Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincidentally with a change in the neurofilament function.

Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization By similarity. Ref.4

Sequence similarities

Belongs to the intermediate filament family.

Ontologies

Keywords
Cellular component	Intermediate filament
Domain	Coiled coil
PTM	Acetylation Glycoprotein Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Cellular_component	axon Inferred from direct assay PubMed 20118926. Source: BHF-UCL cytoskeleton Inferred from direct assay Ref.2. Source: UniProtKB intermediate filament Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	structural molecule activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

Removed

Chain

2 – 846

845

Neurofilament medium polypeptide

PRO_0000063798

Regions

Region

2 – 104

103

Head

Region

104 – 411

308

Rod

Region

104 – 135

Coil 1A

Region

136 – 148

Linker 1

Region

149 – 247

Coil 1B

Region

248 – 264

Linker 12

Region

265 – 286

Coil 2A

Region

287 – 290

Linker 2

Region

291 – 411

121

Coil 2B

Region

412 – 845

434

Tail

Amino acid modifications

Modified residue

N-acetylserine Ref.6

Modified residue

319

Phosphotyrosine By similarity

Modified residue

503

Phosphoserine Ref.4

Modified residue

507

Phosphoserine Ref.4

Modified residue

537

Phosphoserine Ref.4

Modified residue

545

Phosphoserine By similarity

Modified residue

551

Phosphoserine By similarity

Modified residue

604

Phosphoserine Ref.4

Modified residue

609

Phosphoserine Ref.4

Modified residue

643

Phosphoserine By similarity

Modified residue

667

Phosphoserine Ref.4

Modified residue

687

Phosphoserine By similarity

Modified residue

713

Phosphoserine By similarity

Modified residue

767

Phosphoserine By similarity

Glycosylation

O-linked (GlcNAc) Ref.5

CAR_000130

Glycosylation

431

O-linked (GlcNAc) Ref.5

CAR_000131

Experimental info

Sequence conflict

Missing in CAA78136. Ref.2

Sequence conflict

R → P in CAA78136. Ref.2

Sequence conflict

205

V → L in CAA78136. Ref.2

Sequence conflict

501

Missing in AAA41696. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

P12839 [UniParc].

Last modified January 23, 2007. Version 4.
Checksum: 14DE91A1D1F68EC8
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FASTA

846

95,791

        10         20         30         40         50         60 
MSYTLDSLGN PSAYRRVPTE TRSSFSRVSG SPSSGFRSQS WSRGSPSTVS SSYKRSALAP 

        70         80         90        100        110        120 
RLAYSSAMLS SAESSLDFSQ SSSLLNGGSG GDYKLSRSNE KEQLQGLNDR FAGYIEKVHY 

       130        140        150        160        170        180 
LEQQNKEIEA EIHALRQKQA SHAQLGDAYD QEIRELRATL EMVNHEKAQV QLDSDHLEED 

       190        200        210        220        230        240 
IHRLKERFEE EARLRDDTEA AIRAVRKDIE ESSMVKVELD KKVQSLQDEV AFLRSNHEEE 

       250        260        270        280        290        300 
VADLLAQIQA SHITVERKDY LKTDISTALK EIRSQLECHS DQNMHQAEEW FKCRYAKLTE 

       310        320        330        340        350        360 
AAEQNKEAIR SAKEEIAEYR RQLQSKSIEL ESVRGTKESL ERQLSDIEER HNHDLSSYQD 

       370        380        390        400        410        420 
TIQQLENELR GTKWEMARHL REYQDLLNVK MALDIEIAAY RKLLEGEETR FSTFSGSITG 

       430        440        450        460        470        480 
PLYTHRQPSV TISSKIQKTK VEAPKLKVQH KFVEEIIEET KVEDEKSEME DALTVIAEEL 

       490        500        510        520        530        540 
AASAKEEKEE AEEKEEEPEV EKSPVKSPEA KEEEEGEKEE EEEGQEEEEE EDEGVKSDQA 

       550        560        570        580        590        600 
EEGGSEKEGS SEKDEGEQEE EGETEAEGEG EEAEAKEEKK TEGKVEEMAI KEEIKVEKPE 

       610        620        630        640        650        660 
KAKSPVPKSP VEEVKPKPEA KAGKDEQKEE EKVEEKKEVA KESPKEEKVE KKEEKPKDVP 

       670        680        690        700        710        720 
DKKKAESPVK EKAVEEMITI TKSVKVSLEK DTKEEKPQQQ EKVKEKAEEE GGSEEEVGDK 

       730        740        750        760        770        780 
SPQESKKEDI AINGEVEGKE EEEQETQEKG SGQEEEKGVV TNGLDVSPAE EKKGEDRSDD 

       790        800        810        820        830        840 
KVVVTKKVEK ITSEGGDGAT KYITKSVTVT QKVEEHEETF EEKLVSTKKV EKVTSHAIVK 


EVTQGD

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References

[1]	"Complete amino acid sequence and in vitro expression of rat NF-M, the middle molecular weight neurofilament protein." Napolitano E.W., Chin S.S.M., Colman D.R., Liem R.K.H. J. Neurosci. 7:2590-2599(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Schwann cells of the myelin-forming phenotype express neurofilament protein NF-M." Kelly B.M., Gillespie C.S., Sherman D.L., Brophy P.J. J. Cell Biol. 118:397-410(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Wistar.
[3]	Lubec G., Afjehi-Sadat L., Diao W., Kang S.U., Lubec S. Submitted (SEP-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 102-117; 139-154; 168-183; 207-216; 223-258; 352-371; 391-410; 412-427; 452-461 AND 686-693, MASS SPECTROMETRY. Strain: Sprague-Dawley. Tissue: Brain, Hippocampus and Spinal cord.
[4]	"Identification of six phosphorylation sites in the COOH-terminal tail region of the rat neurofilament protein M." Xu Z.-S., Liu W.-S., Willard M.B. J. Biol. Chem. 267:4467-4471(1992) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION AT SER-503; SER-507; SER-537; SER-604; SER-609 AND SER-667, SEQUENCE REVISION TO 500.
[5]	"Glycosylation of mammalian neurofilaments. Localization of multiple O-linked N-acetylglucosamine moieties on neurofilament polypeptides L and M." Dong D.L.-Y., Xu Z.-S., Chevrier M.R., Cotter R.J., Cleveland D.W., Hart G.W. J. Biol. Chem. 268:16679-16687(1993) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION AT THR-48 AND THR-431.
[6]	Lubec G., Chen W.-Q. Submitted (FEB-2007) to UniProtKB Cited for: ACETYLATION AT SER-2, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M18628 mRNA. Translation: AAA41696.1. Z12152 mRNA. Translation: CAA78136.1.
IPI	IPI00325609.
PIR	A45669.
RefSeq	NP_058725.1. NM_017029.2.
UniGene	Rn.10971.
3D structure databases
ProteinModelPortal	P12839.
SMR	P12839. Positions 98-135, 329-406.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-208N.
STRING	10116.ENSRNOP00000018637.
PTM databases
GlycoSuiteDB	P12839.
PhosphoSite	P12839.
2D gel databases
World-2DPAGE	0004:P12839.
Proteomic databases
PaxDb	P12839.
PRIDE	P12839.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	24588.
KEGG	rno:24588.
UCSC	RGD:3160. rat.
Organism-specific databases
CTD	4741.
RGD	3160. Nefm.
Phylogenomic databases
eggNOG	NOG264891.
HOGENOM	HOG000230977.
HOVERGEN	HBG013015.
InParanoid	P12839.
KO	K04573.
OrthoDB	EOG4VMFFD.
Gene expression databases
Genevestigator	P12839.
GermOnline	ENSRNOG00000013916. Rattus norvegicus.
Family and domain databases
InterPro	IPR016044. F. IPR001664. IF. IPR006821. Intermed_filament_DNA-bd. IPR018039. Intermediate_filament_CS. IPR002957. Keratin_I. [Graphical view]
PANTHER	PTHR23239. PTHR23239. 1 hit.
Pfam	PF00038. Filament. 1 hit. PF04732. Filament_head. 1 hit. [Graphical view]
PRINTS	PR01248. TYPE1KERATIN.
PROSITE	PS00226. IF. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	603770.

Entry information

Entry name

NFM_RAT

Accession

Primary (citable) accession number: P12839
Secondary accession number(s): Q63370

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 115 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

2D gel databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents