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Protein

Neutrophil defensin 4

Gene

DEFA4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Has antimicrobial activity against Gram-negative bacteria, and to a lesser extent also against Gram-positive bacteria and fungi. Protects blood cells against infection with HIV-1 (in vitro). Inhibits corticotropin (ACTH)-stimulated corticosterone production.2 Publications

GO - Molecular functioni

  • protein homodimerization activity Source: UniProtKB

GO - Biological processi

  • antibacterial humoral response Source: UniProtKB
  • antifungal humoral response Source: UniProtKB
  • antimicrobial humoral immune response mediated by antimicrobial peptide Source: UniProtKB
  • antimicrobial humoral response Source: UniProtKB
  • defense response to fungus Source: UniProtKB
  • defense response to Gram-negative bacterium Source: UniProtKB
  • defense response to Gram-positive bacterium Source: UniProtKB
  • innate immune response Source: UniProtKB
  • killing of cells of other organism Source: UniProtKB
  • neutrophil degranulation Source: Reactome
  • T cell chemotaxis Source: UniProtKB

Keywordsi

Molecular functionAntibiotic, Antimicrobial, Defensin, Fungicide

Enzyme and pathway databases

ReactomeiR-HSA-1461973. Defensins.
R-HSA-1462054. Alpha-defensins.
R-HSA-6798695. Neutrophil degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Neutrophil defensin 4
Alternative name(s):
Defensin, alpha 4
HNP-4
Short name:
HP-4
Gene namesi
Name:DEFA4
Synonyms:DEF4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 8

Organism-specific databases

EuPathDBiHostDB:ENSG00000164821.4.
HGNCiHGNC:2763. DEFA4.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

DisGeNETi1669.
OpenTargetsiENSG00000164821.
PharmGKBiPA27240.

Polymorphism and mutation databases

BioMutaiDEFA4.
DMDMi399352.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
PropeptideiPRO_000000678120 – 63Add BLAST44
PeptideiPRO_000000678264 – 96Neutrophil defensin 4Add BLAST33
PropeptideiPRO_0000006783971

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi65 ↔ 931 Publication
Disulfide bondi67 ↔ 821 Publication
Disulfide bondi72 ↔ 921 Publication

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP12838.
PeptideAtlasiP12838.
PRIDEiP12838.

Expressioni

Gene expression databases

BgeeiENSG00000164821.
CleanExiHS_DEFA4.
GenevisibleiP12838. HS.

Organism-specific databases

HPAiHPA051266.

Interactioni

Subunit structurei

Homodimer.1 Publication

GO - Molecular functioni

  • protein homodimerization activity Source: UniProtKB

Protein-protein interaction databases

IntActiP12838. 1 interactor.
STRINGi9606.ENSP00000297435.

Structurei

Secondary structure

197
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi65 – 70Combined sources6
Beta strandi77 – 84Combined sources8
Beta strandi87 – 93Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1ZMMX-ray1.60A/B/C/D64-96[»]
ProteinModelPortaliP12838.
SMRiP12838.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP12838.

Family & Domainsi

Sequence similaritiesi

Belongs to the alpha-defensin family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410JK0K. Eukaryota.
ENOG41116GP. LUCA.
GeneTreeiENSGT00730000111183.
HOGENOMiHOG000233351.
HOVERGENiHBG011703.
InParanoidiP12838.
KOiK05230.
OMAiSFTYCCT.
OrthoDBiEOG091G12GE.
PhylomeDBiP12838.
TreeFamiTF338414.

Family and domain databases

InterProiView protein in InterPro
IPR006081. Alpha-defensin.
IPR016327. Alpha-defensin_pro.
IPR006080. Defensin_beta/alpha.
IPR002366. Defensin_propep.
PANTHERiPTHR11876. PTHR11876. 1 hit.
PfamiView protein in Pfam
PF00323. Defensin_1. 1 hit.
PF00879. Defensin_propep. 1 hit.
PIRSFiPIRSF001875. Alpha-defensin. 1 hit.
SMARTiView protein in SMART
SM00048. DEFSN. 1 hit.
PROSITEiView protein in PROSITE
PS00269. DEFENSIN. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P12838-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRIIALLAAI LLVALQVRAG PLQARGDEAP GQEQRGPEDQ DISISFAWDK
60 70 80 90
SSALQVSGST RGMVCSCRLV FCRRTELRVG NCLIGGVSFT YCCTRVD
Length:97
Mass (Da):10,504
Last modified:July 1, 1993 - v2
Checksum:iFE14334631EC2FD3
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_0488618A → P. Corresponds to variant dbSNP:rs28661751Ensembl.1
Natural variantiVAR_0611328A → V. Corresponds to variant dbSNP:rs28488529Ensembl.1
Natural variantiVAR_03631574R → Q in a colorectal cancer sample; somatic mutation. 1 PublicationCorresponds to variant dbSNP:rs762433303Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18745 Genomic DNA. Translation: AAA64488.1.
AF200455 Genomic DNA. Translation: AAT68877.1.
CH471153 Genomic DNA. Translation: EAW80486.1.
BC093959 mRNA. Translation: AAH93959.1.
BC106747 mRNA. Translation: AAI06748.1.
BC112091 mRNA. Translation: AAI12092.1.
X65977 mRNA. Translation: CAA46792.1.
CCDSiCCDS5961.1.
PIRiA47365.
RefSeqiNP_001916.1. NM_001925.2.
UniGeneiHs.591391.

Genome annotation databases

EnsembliENST00000297435; ENSP00000297435; ENSG00000164821.
GeneIDi1669.
KEGGihsa:1669.
UCSCiuc003wqu.2. human.

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiDEF4_HUMAN
AccessioniPrimary (citable) accession number: P12838
Secondary accession number(s): Q6EZF8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: July 1, 1993
Last modified: September 27, 2017
This is version 147 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families