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Protein

Neutrophil defensin 4

Gene

DEFA4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Has antimicrobial activity against Gram-negative bacteria, and to a lesser extent also against Gram-positive bacteria and fungi. Protects blood cells against infection with HIV-1 (in vitro). Inhibits corticotropin (ACTH)-stimulated corticosterone production.2 Publications

GO - Biological processi

  • antibacterial humoral response Source: UniProtKB
  • antimicrobial humoral response Source: UniProtKB
  • defense response to fungus Source: UniProtKB-KW
  • defense response to Gram-negative bacterium Source: UniProtKB
  • defense response to Gram-positive bacterium Source: UniProtKB
  • innate immune response Source: UniProtKB
  • killing of cells of other organism Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Antibiotic, Antimicrobial, Defensin, Fungicide

Enzyme and pathway databases

ReactomeiR-HSA-1461973. Defensins.
R-HSA-1462054. Alpha-defensins.

Names & Taxonomyi

Protein namesi
Recommended name:
Neutrophil defensin 4
Alternative name(s):
Defensin, alpha 4
HNP-4
Short name:
HP-4
Gene namesi
Name:DEFA4
Synonyms:DEF4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 8

Organism-specific databases

HGNCiHGNC:2763. DEFA4.

Subcellular locationi

GO - Cellular componenti

  • azurophil granule Source: UniProtKB
  • extracellular region Source: Reactome
  • extracellular space Source: InterPro
  • Golgi lumen Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA27240.

Polymorphism and mutation databases

BioMutaiDEFA4.
DMDMi399352.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Sequence analysisAdd
BLAST
Propeptidei20 – 6344PRO_0000006781Add
BLAST
Peptidei64 – 9633Neutrophil defensin 4PRO_0000006782Add
BLAST
Propeptidei97 – 971PRO_0000006783

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi65 ↔ 931 Publication
Disulfide bondi67 ↔ 821 Publication
Disulfide bondi72 ↔ 921 Publication

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP12838.
PeptideAtlasiP12838.
PRIDEiP12838.

Expressioni

Gene expression databases

BgeeiENSG00000164821.
CleanExiHS_DEFA4.
GenevisibleiP12838. HS.

Organism-specific databases

HPAiHPA051266.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

IntActiP12838. 1 interaction.
STRINGi9606.ENSP00000297435.

Structurei

Secondary structure

1
97
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi65 – 706Combined sources
Beta strandi77 – 848Combined sources
Beta strandi87 – 937Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ZMMX-ray1.60A/B/C/D64-96[»]
ProteinModelPortaliP12838.
SMRiP12838. Positions 64-96.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP12838.

Family & Domainsi

Sequence similaritiesi

Belongs to the alpha-defensin family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410JK0K. Eukaryota.
ENOG41116GP. LUCA.
GeneTreeiENSGT00730000111183.
HOGENOMiHOG000233351.
HOVERGENiHBG011703.
InParanoidiP12838.
KOiK05230.
OMAiSFTYCCT.
OrthoDBiEOG091G12GE.
PhylomeDBiP12838.
TreeFamiTF338414.

Family and domain databases

InterProiIPR006081. Alpha-defensin.
IPR016327. Alpha-defensin_pro.
IPR006080. Defensin_beta/alpha.
IPR002366. Defensin_propep.
[Graphical view]
PANTHERiPTHR11876. PTHR11876. 1 hit.
PfamiPF00323. Defensin_1. 1 hit.
PF00879. Defensin_propep. 1 hit.
[Graphical view]
PIRSFiPIRSF001875. Alpha-defensin. 1 hit.
SMARTiSM00048. DEFSN. 1 hit.
[Graphical view]
PROSITEiPS00269. DEFENSIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P12838-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRIIALLAAI LLVALQVRAG PLQARGDEAP GQEQRGPEDQ DISISFAWDK
60 70 80 90
SSALQVSGST RGMVCSCRLV FCRRTELRVG NCLIGGVSFT YCCTRVD
Length:97
Mass (Da):10,504
Last modified:July 1, 1993 - v2
Checksum:iFE14334631EC2FD3
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti8 – 81A → P.
Corresponds to variant rs28661751 [ dbSNP | Ensembl ].
VAR_048861
Natural varianti8 – 81A → V.
Corresponds to variant rs28488529 [ dbSNP | Ensembl ].
VAR_061132
Natural varianti74 – 741R → Q in a colorectal cancer sample; somatic mutation. 1 Publication
Corresponds to variant rs762433303 [ dbSNP | Ensembl ].
VAR_036315

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18745 Genomic DNA. Translation: AAA64488.1.
AF200455 Genomic DNA. Translation: AAT68877.1.
CH471153 Genomic DNA. Translation: EAW80486.1.
BC093959 mRNA. Translation: AAH93959.1.
BC106747 mRNA. Translation: AAI06748.1.
BC112091 mRNA. Translation: AAI12092.1.
X65977 mRNA. Translation: CAA46792.1.
CCDSiCCDS5961.1.
PIRiA47365.
RefSeqiNP_001916.1. NM_001925.2.
UniGeneiHs.591391.

Genome annotation databases

EnsembliENST00000297435; ENSP00000297435; ENSG00000164821.
GeneIDi1669.
KEGGihsa:1669.
UCSCiuc003wqu.2. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18745 Genomic DNA. Translation: AAA64488.1.
AF200455 Genomic DNA. Translation: AAT68877.1.
CH471153 Genomic DNA. Translation: EAW80486.1.
BC093959 mRNA. Translation: AAH93959.1.
BC106747 mRNA. Translation: AAI06748.1.
BC112091 mRNA. Translation: AAI12092.1.
X65977 mRNA. Translation: CAA46792.1.
CCDSiCCDS5961.1.
PIRiA47365.
RefSeqiNP_001916.1. NM_001925.2.
UniGeneiHs.591391.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ZMMX-ray1.60A/B/C/D64-96[»]
ProteinModelPortaliP12838.
SMRiP12838. Positions 64-96.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP12838. 1 interaction.
STRINGi9606.ENSP00000297435.

Polymorphism and mutation databases

BioMutaiDEFA4.
DMDMi399352.

Proteomic databases

PaxDbiP12838.
PeptideAtlasiP12838.
PRIDEiP12838.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000297435; ENSP00000297435; ENSG00000164821.
GeneIDi1669.
KEGGihsa:1669.
UCSCiuc003wqu.2. human.

Organism-specific databases

CTDi1669.
GeneCardsiDEFA4.
HGNCiHGNC:2763. DEFA4.
HPAiHPA051266.
MIMi601157. gene.
neXtProtiNX_P12838.
PharmGKBiPA27240.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410JK0K. Eukaryota.
ENOG41116GP. LUCA.
GeneTreeiENSGT00730000111183.
HOGENOMiHOG000233351.
HOVERGENiHBG011703.
InParanoidiP12838.
KOiK05230.
OMAiSFTYCCT.
OrthoDBiEOG091G12GE.
PhylomeDBiP12838.
TreeFamiTF338414.

Enzyme and pathway databases

ReactomeiR-HSA-1461973. Defensins.
R-HSA-1462054. Alpha-defensins.

Miscellaneous databases

EvolutionaryTraceiP12838.
GeneWikiiDEFA4.
GenomeRNAii1669.
PROiP12838.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000164821.
CleanExiHS_DEFA4.
GenevisibleiP12838. HS.

Family and domain databases

InterProiIPR006081. Alpha-defensin.
IPR016327. Alpha-defensin_pro.
IPR006080. Defensin_beta/alpha.
IPR002366. Defensin_propep.
[Graphical view]
PANTHERiPTHR11876. PTHR11876. 1 hit.
PfamiPF00323. Defensin_1. 1 hit.
PF00879. Defensin_propep. 1 hit.
[Graphical view]
PIRSFiPIRSF001875. Alpha-defensin. 1 hit.
SMARTiSM00048. DEFSN. 1 hit.
[Graphical view]
PROSITEiPS00269. DEFENSIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDEF4_HUMAN
AccessioniPrimary (citable) accession number: P12838
Secondary accession number(s): Q6EZF8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: July 1, 1993
Last modified: September 7, 2016
This is version 139 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 8
    Human chromosome 8: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.