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P12833

- DYR3_SALTM

UniProt

P12833 - DYR3_SALTM

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Protein

Dihydrofolate reductase type 3

Gene

dhfrIII

Organism
Salmonella typhimurium
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis (By similarity).By similarity

Catalytic activityi

5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.PROSITE-ProRule annotation

Pathwayi

GO - Molecular functioni

  1. dihydrofolate reductase activity Source: UniProtKB-EC
  2. NADP binding Source: InterPro

GO - Biological processi

  1. glycine biosynthetic process Source: InterPro
  2. nucleotide biosynthetic process Source: InterPro
  3. one-carbon metabolic process Source: UniProtKB-KW
  4. response to antibiotic Source: UniProtKB-KW
  5. response to methotrexate Source: UniProtKB-KW
  6. tetrahydrofolate biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Antibiotic resistance, Methotrexate resistance, One-carbon metabolism, Trimethoprim resistance

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciRETL1328306-WGS:GSTH-2839-MONOMER.
UniPathwayiUPA00077; UER00158.

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydrofolate reductase type 3 (EC:1.5.1.3)
Alternative name(s):
Dihydrofolate reductase type III
Gene namesi
Name:dhfrIII
Encoded oniPlasmid pAZ10 Publication
OrganismiSalmonella typhimurium
Taxonomic identifieri90371 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSalmonella

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 162162Dihydrofolate reductase type 3PRO_0000186421Add
BLAST

Interactioni

Subunit structurei

Monomer.

Structurei

3D structure databases

ProteinModelPortaliP12833.
SMRiP12833. Positions 2-161.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 160159DHFRPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the dihydrofolate reductase family.Curated
Contains 1 DHFR (dihydrofolate reductase) domain.PROSITE-ProRule annotation

Family and domain databases

Gene3Di3.40.430.10. 1 hit.
InterProiIPR012259. DHFR.
IPR024072. DHFR-like_dom.
IPR017925. DHFR_CS.
IPR001796. DHFR_dom.
[Graphical view]
PfamiPF00186. DHFR_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000194. DHFR. 1 hit.
PRINTSiPR00070. DHFR.
SUPFAMiSSF53597. SSF53597. 1 hit.
PROSITEiPS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P12833-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLISLIAALA HNNLIGKDNL IPWHLPADLR HFKAVTLGKP VVMGRRTFES
60 70 80 90 100
IGRPLPGRRN VVVSRNPQWQ AEGVEVAPSL DAALALLTDC EEAMIIGGGQ
110 120 130 140 150
LYAEALPRAD RLYLTYIDAQ LNGDTHFPDY LSLGWQELER STHPADDKNS
160
YACEFVTLSR QR
Length:162
Mass (Da):18,033
Last modified:October 1, 1989 - v1
Checksum:i199343AE8675FDED
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti8 – 81A → S AA sequence (PubMed:6371010)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J03306 Genomic DNA. Translation: AAA25550.1.
PIRiB22241.
JT0266. RDEBDT.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J03306 Genomic DNA. Translation: AAA25550.1 .
PIRi B22241.
JT0266. RDEBDT.

3D structure databases

ProteinModelPortali P12833.
SMRi P12833. Positions 2-161.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00077 ; UER00158 .
BioCyci RETL1328306-WGS:GSTH-2839-MONOMER.

Family and domain databases

Gene3Di 3.40.430.10. 1 hit.
InterProi IPR012259. DHFR.
IPR024072. DHFR-like_dom.
IPR017925. DHFR_CS.
IPR001796. DHFR_dom.
[Graphical view ]
Pfami PF00186. DHFR_1. 1 hit.
[Graphical view ]
PIRSFi PIRSF000194. DHFR. 1 hit.
PRINTSi PR00070. DHFR.
SUPFAMi SSF53597. SSF53597. 1 hit.
PROSITEi PS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Characterization of plasmid pAZ1 and the type III dihydrofolate reductase gene."
    Fling M.E., Kopf J., Richards C.
    Plasmid 19:30-38(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Characterization of an R-plasmid dihydrofolate reductase with a monomeric structure."
    Joyner S.S., Fling M.E., Stone D., Baccanari D.P.
    J. Biol. Chem. 259:5851-5856(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-21.

Entry informationi

Entry nameiDYR3_SALTM
AccessioniPrimary (citable) accession number: P12833
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 1, 1989
Last modified: October 1, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The plasmid pAZ1 determines trimethoprim and sulphonamide resistance.

Keywords - Technical termi

Direct protein sequencing, Plasmid

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3