P12829 (MYL4_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Myosin light chain 4

Alternative name(s):
Myosin light chain 1, embryonic muscle/atrial isoform
Myosin light chain alkali GT-1 isoform

Gene names

Name:	MYL4
Synonyms:	MLC1
ORF Names:	PRO1957

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	197 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Regulatory light chain of myosin. Does not bind calcium.
Subunit structure	Myosin is a hexamer of 2 heavy chains and 4 light chains.
Sequence similarities	Contains 3 EF-hand domains.

Ontologies

Keywords
Coding sequence diversity	Polymorphism
Domain	Repeat
Molecular function	Motor protein Muscle protein Myosin
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cardiac muscle contraction Inferred from mutant phenotype PubMed 14985854PubMed 16675844. Source: BHF-UCL muscle filament sliding Traceable author statement. Source: Reactome muscle organ development Non-traceable author statement. Source: UniProtKB positive regulation of ATPase activity Inferred from mutant phenotype PubMed 9738905. Source: BHF-UCL regulation of the force of heart contraction Inferred from mutant phenotype PubMed 16675844. Source: BHF-UCL
Cellular_component	A band Inferred from mutant phenotype PubMed 14985854. Source: BHF-UCL cytosol Traceable author statement. Source: Reactome muscle myosin complex Non-traceable author statement. Source: UniProtKB
Molecular_function	actin filament binding Inferred from mutant phenotype PubMed 9738905. Source: BHF-UCL actin monomer binding Inferred from direct assay PubMed 9180271. Source: BHF-UCL calcium ion binding Inferred from electronic annotation. Source: InterPro myosin II heavy chain binding Non-traceable author statement PubMed 16675844. Source: BHF-UCL structural constituent of muscle Non-traceable author statement. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 197	196	Myosin light chain 4		PRO_0000198699
Regions
	Domain	51 – 88	38	EF-hand 1
	Domain	130 – 165	36	EF-hand 2
	Domain	165 – 197	33	EF-hand 3
Natural variations
	Natural variant	186	1	N → Y. Corresponds to variant rs16941677 [ dbSNP | Ensembl ].		VAR_050458
Experimental info
	Sequence conflict	51	1	D → E in AAA59891. Ref.2
	Sequence conflict	56	1	F → L in AAA59891. Ref.2
	Sequence conflict	119	1	I → V in AAA59856. Ref.9
	Sequence conflict	143	1	D → E in AAA59891. Ref.2
	Sequence conflict	150	1	V → D in AAA59891. Ref.2
	Sequence conflict	181	1	A → V in AAA59856. Ref.9
	Sequence conflict	185	1	I → V in AAA59856. Ref.9

Sequences

	Sequence		Length	Mass (Da)
	P12829 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: FB147B49E8506E6C	FASTA	197	21,565
	10 20 30 40 50 60 MAPKKPEPKK EAAKPAPAPA PAPAPAPAPA PEAPKEPAFD PKSVKIDFTA DQIEEFKEAF 70 80 90 100 110 120 SLFDRTPTGE MKITYGQCGD VLRALGQNPT NAEVLRVLGK PKPEEMNVKM LDFETFLPIL 130 140 150 160 170 180 QHISRNKEQG TYEDFVEGLR VFDKESNGTV MGAELRHVLA TLGEKMTEAE VEQLLAGQED 190 ANGCINYEAF VKHIMSG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel human myosin alkali light chain is developmentally regulated. Expression in fetal cardiac and skeletal muscle and in adult atria." Arnold H.H., Lohse P., Seidel U., Bober E. Eur. J. Biochem. 178:53-60(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Skeletal muscle.
[2]	"Molecular cloning and characterization of human atrial and ventricular myosin alkali light chain cDNA clones." Kurabayashi M., Komuro I., Tsuchimochi H., Takaku F., Yazaki Y. J. Biol. Chem. 263:13930-13936(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Human embryonic/atrial myosin alkali light chain gene: characterization, sequence, and chromosomal location." Seharaseyon J., Bober E., Hsieh C.L., Fodor W.L., Francke U., Arnold H.H., Vanin E.F. Genomics 7:289-293(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"Heterogenic mRNAs with an identical protein-coding region of the human embryonic myosin alkali light chain in skeletal muscle cells." Zimmermann K., Kautz S., Hajdu G., Winter C., Whalen R.G., Starzinski-Powitz A. J. Mol. Biol. 211:505-513(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[5]	"The human embryonic myosin alkali light chain gene: use of alternative promoters and 3' non-coding regions." Rotter M., Zimmerman K., Poustka A., Starzinski-Powitz A. Nucleic Acids Res. 19:1497-1504(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Blood.
[6]	"Functional prediction of the coding sequences of 121 new genes deduced by analysis of cDNA clones from human fetal liver." Zhang C., Yu Y., Zhang S., Wei H., Zhou G., Ouyang S., Luo L., Bi J., Liu M., He F. Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Fetal liver.
[7]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Pancreas and Spleen.
[9]	"Alkali myosin light chains in man are encoded by a multigene family that includes the adult skeletal muscle, the embryonic or atrial, and nonsarcomeric isoforms." Seidel U., Bober E., Winter B., Lenz S., Lohse P., Goedde H., Grzeschik K., Arnold H.H. Gene 66:135-146(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 104-197.
[10]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M36172 mRNA. Translation: AAA36319.1. X13955 mRNA. Translation: CAA32137.1. M24121 mRNA. Translation: AAA59891.1. M37074 , M37069, M37070, M37071, M37072, M37073 Genomic DNA. Translation: AAA59858.1. X58851 , X58852, X58853, X58854 Genomic DNA. Translation: CAA41655.1. AF116676 mRNA. Translation: AAF71096.1. CH471231 Genomic DNA. Translation: EAW57684.1. CH471231 Genomic DNA. Translation: EAW57685.1. BC030228 mRNA. Translation: AAH30228.1. M20641 mRNA. Translation: AAA59856.1.
IPI	IPI00384992.
PIR	MOHU4E. A32730.
RefSeq	NP_001002841.1. NM_001002841.1. NP_002467.1. NM_002476.2.
UniGene	Hs.463300.
3D structure databases
ProteinModelPortal	P12829.
ModBase	Search...
Protein-protein interaction databases
IntAct	P12829. 4 interactions.
STRING	9606.ENSP00000347055.
PTM databases
PhosphoSite	P12829.
Polymorphism databases
DMDM	127138.
2D gel databases
UCD-2DPAGE	P12829.
Proteomic databases
PaxDb	P12829.
PeptideAtlas	P12829.
PRIDE	P12829.
Protocols and materials databases
DNASU	4635.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000354968; ENSP00000347055; ENSG00000198336. ENST00000393450; ENSP00000377096; ENSG00000198336. ENST00000536623; ENSP00000442375; ENSG00000198336. ENST00000572316; ENSP00000461570; ENSG00000198336.
GeneID	4635.
KEGG	hsa:4635.
UCSC	uc002ilg.3. human.
Organism-specific databases
CTD	4635.
GeneCards	GC17P045286.
HGNC	HGNC:7585. MYL4.
HPA	HPA051884.
MIM	160770. gene.
neXtProt	NX_P12829.
PharmGKB	PA31382.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG5126.
HOGENOM	HOG000233018.
HOVERGEN	HBG012180.
InParanoid	P12829.
KO	K12750.
OMA	HISRNKD.
OrthoDB	EOG483D5S.
Enzyme and pathway databases
Reactome	REACT_17044. Muscle contraction.
Gene expression databases
ArrayExpress	P12829.
Bgee	P12829.
CleanEx	HS_MLC1. HS_MYL4.
Genevestigator	P12829.
GermOnline	ENSG00000198336. Homo sapiens.
Family and domain databases
Gene3D	1.10.238.10. 2 hits.
InterPro	IPR011992. EF-hand-like_dom. IPR002048. EF_hand_dom. [Graphical view]
Pfam	PF13405. EF_hand_4. 1 hit. [Graphical view]
PROSITE	PS50222. EF_HAND_2. 2 hits. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	4635.
NextBio	17844.
SOURCE	Search...

Entry information

Entry name

MYL4_HUMAN

Accession

Primary (citable) accession number: P12829
Secondary accession number(s): D3DXJ7, P11783

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 126 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.