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Protein

Myosin light chain 4

Gene

MYL4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulatory light chain of myosin. Does not bind calcium.

GO - Molecular functioni

  • actin filament binding Source: BHF-UCL
  • actin monomer binding Source: BHF-UCL
  • calcium ion binding Source: InterPro
  • myosin II heavy chain binding Source: BHF-UCL

GO - Biological processi

  • cardiac muscle contraction Source: BHF-UCL
  • muscle filament sliding Source: Reactome
  • positive regulation of ATPase activity Source: BHF-UCL
  • regulation of the force of heart contraction Source: BHF-UCL
Complete GO annotation...

Keywords - Molecular functioni

Motor protein, Muscle protein, Myosin

Enzyme and pathway databases

ReactomeiR-HSA-390522. Striated Muscle Contraction.
SIGNORiP12829.

Names & Taxonomyi

Protein namesi
Recommended name:
Myosin light chain 4
Alternative name(s):
Myosin light chain 1, embryonic muscle/atrial isoform
Myosin light chain alkali GT-1 isoform
Gene namesi
Name:MYL4
Synonyms:MLC1
ORF Names:PRO1957
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:7585. MYL4.

Subcellular locationi

GO - Cellular componenti

  • A band Source: BHF-UCL
  • cytosol Source: Reactome
  • myosin complex Source: UniProtKB-KW
Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

DisGeNETi4635.
OpenTargetsiENSG00000198336.
PharmGKBiPA31382.

Polymorphism and mutation databases

BioMutaiMYL4.
DMDMi127138.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001986992 – 197Myosin light chain 4Add BLAST196

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N,N,N-trimethylalanineBy similarity1
Modified residuei74PhosphothreonineBy similarity1
Modified residuei90PhosphothreonineBy similarity1

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

EPDiP12829.
MaxQBiP12829.
PaxDbiP12829.
PeptideAtlasiP12829.
PRIDEiP12829.

2D gel databases

UCD-2DPAGEP12829.

PTM databases

iPTMnetiP12829.
PhosphoSitePlusiP12829.

Expressioni

Gene expression databases

BgeeiENSG00000198336.
CleanExiHS_MLC1.
HS_MYL4.
ExpressionAtlasiP12829. baseline and differential.
GenevisibleiP12829. HS.

Organism-specific databases

HPAiHPA046859.
HPA051884.

Interactioni

Subunit structurei

Myosin is a hexamer of 2 heavy chains and 4 light chains.

GO - Molecular functioni

  • actin filament binding Source: BHF-UCL
  • actin monomer binding Source: BHF-UCL
  • myosin II heavy chain binding Source: BHF-UCL

Protein-protein interaction databases

BioGridi110719. 5 interactors.
IntActiP12829. 4 interactors.
STRINGi9606.ENSP00000347055.

Structurei

3D structure databases

ProteinModelPortaliP12829.
SMRiP12829.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini51 – 88EF-hand 1PROSITE-ProRule annotationAdd BLAST38
Domaini130 – 165EF-hand 2PROSITE-ProRule annotationAdd BLAST36

Sequence similaritiesi

Contains 2 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0030. Eukaryota.
COG5126. LUCA.
GeneTreeiENSGT00590000082921.
HOGENOMiHOG000233018.
HOVERGENiHBG012180.
InParanoidiP12829.
KOiK12750.
OMAiPREVDYK.
OrthoDBiEOG091G0TQL.
PhylomeDBiP12829.
TreeFamiTF351553.

Family and domain databases

CDDicd00051. EFh. 1 hit.
Gene3Di1.10.238.10. 2 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR002048. EF_hand_dom.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS50222. EF_HAND_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P12829-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPKKPEPKK EAAKPAPAPA PAPAPAPAPA PEAPKEPAFD PKSVKIDFTA
60 70 80 90 100
DQIEEFKEAF SLFDRTPTGE MKITYGQCGD VLRALGQNPT NAEVLRVLGK
110 120 130 140 150
PKPEEMNVKM LDFETFLPIL QHISRNKEQG TYEDFVEGLR VFDKESNGTV
160 170 180 190
MGAELRHVLA TLGEKMTEAE VEQLLAGQED ANGCINYEAF VKHIMSG
Length:197
Mass (Da):21,565
Last modified:January 23, 2007 - v3
Checksum:iFB147B49E8506E6C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti51D → E in AAA59891 (PubMed:3417683).Curated1
Sequence conflicti56F → L in AAA59891 (PubMed:3417683).Curated1
Sequence conflicti119I → V in AAA59856 (PubMed:2458299).Curated1
Sequence conflicti143D → E in AAA59891 (PubMed:3417683).Curated1
Sequence conflicti150V → D in AAA59891 (PubMed:3417683).Curated1
Sequence conflicti181A → V in AAA59856 (PubMed:2458299).Curated1
Sequence conflicti185I → V in AAA59856 (PubMed:2458299).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_050458186N → Y.Corresponds to variant rs16941677dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M36172 mRNA. Translation: AAA36319.1.
X13955 mRNA. Translation: CAA32137.1.
M24121 mRNA. Translation: AAA59891.1.
M37074
, M37069, M37070, M37071, M37072, M37073 Genomic DNA. Translation: AAA59858.1.
X58851
, X58852, X58853, X58854 Genomic DNA. Translation: CAA41655.1.
AF116676 mRNA. Translation: AAF71096.1.
CH471231 Genomic DNA. Translation: EAW57684.1.
CH471231 Genomic DNA. Translation: EAW57685.1.
BC030228 mRNA. Translation: AAH30228.1.
M20641 mRNA. Translation: AAA59856.1.
CCDSiCCDS11510.1.
PIRiA32730. MOHU4E.
RefSeqiNP_001002841.1. NM_001002841.1.
NP_002467.1. NM_002476.2.
XP_005257448.1. XM_005257391.4.
UniGeneiHs.463300.

Genome annotation databases

EnsembliENST00000354968; ENSP00000347055; ENSG00000198336.
ENST00000393450; ENSP00000377096; ENSG00000198336.
ENST00000572316; ENSP00000461570; ENSG00000198336.
GeneIDi4635.
KEGGihsa:4635.
UCSCiuc002ilg.4. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M36172 mRNA. Translation: AAA36319.1.
X13955 mRNA. Translation: CAA32137.1.
M24121 mRNA. Translation: AAA59891.1.
M37074
, M37069, M37070, M37071, M37072, M37073 Genomic DNA. Translation: AAA59858.1.
X58851
, X58852, X58853, X58854 Genomic DNA. Translation: CAA41655.1.
AF116676 mRNA. Translation: AAF71096.1.
CH471231 Genomic DNA. Translation: EAW57684.1.
CH471231 Genomic DNA. Translation: EAW57685.1.
BC030228 mRNA. Translation: AAH30228.1.
M20641 mRNA. Translation: AAA59856.1.
CCDSiCCDS11510.1.
PIRiA32730. MOHU4E.
RefSeqiNP_001002841.1. NM_001002841.1.
NP_002467.1. NM_002476.2.
XP_005257448.1. XM_005257391.4.
UniGeneiHs.463300.

3D structure databases

ProteinModelPortaliP12829.
SMRiP12829.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi110719. 5 interactors.
IntActiP12829. 4 interactors.
STRINGi9606.ENSP00000347055.

PTM databases

iPTMnetiP12829.
PhosphoSitePlusiP12829.

Polymorphism and mutation databases

BioMutaiMYL4.
DMDMi127138.

2D gel databases

UCD-2DPAGEP12829.

Proteomic databases

EPDiP12829.
MaxQBiP12829.
PaxDbiP12829.
PeptideAtlasiP12829.
PRIDEiP12829.

Protocols and materials databases

DNASUi4635.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000354968; ENSP00000347055; ENSG00000198336.
ENST00000393450; ENSP00000377096; ENSG00000198336.
ENST00000572316; ENSP00000461570; ENSG00000198336.
GeneIDi4635.
KEGGihsa:4635.
UCSCiuc002ilg.4. human.

Organism-specific databases

CTDi4635.
DisGeNETi4635.
GeneCardsiMYL4.
HGNCiHGNC:7585. MYL4.
HPAiHPA046859.
HPA051884.
MIMi160770. gene.
neXtProtiNX_P12829.
OpenTargetsiENSG00000198336.
PharmGKBiPA31382.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0030. Eukaryota.
COG5126. LUCA.
GeneTreeiENSGT00590000082921.
HOGENOMiHOG000233018.
HOVERGENiHBG012180.
InParanoidiP12829.
KOiK12750.
OMAiPREVDYK.
OrthoDBiEOG091G0TQL.
PhylomeDBiP12829.
TreeFamiTF351553.

Enzyme and pathway databases

ReactomeiR-HSA-390522. Striated Muscle Contraction.
SIGNORiP12829.

Miscellaneous databases

GeneWikiiMYL4.
GenomeRNAii4635.
PROiP12829.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000198336.
CleanExiHS_MLC1.
HS_MYL4.
ExpressionAtlasiP12829. baseline and differential.
GenevisibleiP12829. HS.

Family and domain databases

CDDicd00051. EFh. 1 hit.
Gene3Di1.10.238.10. 2 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR002048. EF_hand_dom.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS50222. EF_HAND_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMYL4_HUMAN
AccessioniPrimary (citable) accession number: P12829
Secondary accession number(s): D3DXJ7, P11783
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 159 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.