P12820 (ACE_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Angiotensin-converting enzyme
Short name=ACE
EC=3.2.1.-
EC=3.4.15.1

Alternative name(s):
Dipeptidyl carboxypeptidase I
Kininase II
CD_antigen=CD143

Gene names

Name:	ACE
Synonyms:	DCP1

Organism

Bos taurus (Bovine) [Reference proteome]

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	100 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety By similarity.
Catalytic activity	Release of a C-terminal dipeptide, oligopeptide-|-Xaa-Yaa, when Xaa is not Pro, and Yaa is neither Asp nor Glu. Thus, conversion of angiotensin I to angiotensin II, with increase in vasoconstrictor activity, but no action on angiotensin II.
Cofactor	Binds 2 zinc ions per subunit By similarity. Binds 2 chloride ions per subunit By similarity.
Subcellular location	Secreted.
Sequence similarities	Belongs to the peptidase M2 family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Ligand	Zinc
Molecular function	Carboxypeptidase Hydrolase Metalloprotease Protease
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell membrane Inferred from electronic annotation. Source: InterPro
Molecular_function	carboxypeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW metallopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW peptidyl-dipeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 28	28	Ref.2 Ref.3
	Chain	29 – ›100	›72	Angiotensin-converting enzyme		PRO_0000078151
Regions
	Topological domain	29 – ›100	›72	Extracellular Potential
	Region	29 – ›100	›72	Peptidase M2 1
Experimental info
	Non-terminal residue	100	1

Sequences

	Sequence		Length	Mass (Da)
	P12820 [UniParc]. Last modified September 2, 2008. Version 2. Checksum: F83F87B94EAFEC59	FASTA	100	10,681
	10 20 30 40 50 60 MGAASGRRSP PLLLPLLLLL LPPPPVILEL DPALQPGNFP ADEAGAQIFA ASFNSSAEQV 70 80 90 100 LFQSTAASWA HDTNITEENA RLQEEAALLS QEFSEAWGQK

References

[1]	"Analysis of the angiotensin converting enzyme promoter from Bos taurus." Mungunsukh O., Day R.M. Submitted (NOV-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Bovine angiotensin-converting enzyme: amino-terminal sequence analysis and preliminary characterization of a hybridization-selected primary translation product." St Clair D.K., Presper K.A., Smith P.L., Stump D.C., Heath E.C. Biochem. Biophys. Res. Commun. 141:968-972(1986) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 29-50. Tissue: Lung.
[3]	"Partial protein sequence of mouse and bovine kidney angiotensin converting enzyme." Bernstein K.E., Martin B.M., Striker L., Striker G. Kidney Int. 33:652-655(1988) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 29-50.

Cross-references

Sequence databases
EMBL GenBank DDBJ	DQ885942 Genomic DNA. Translation: ABI35897.2.
IPI	IPI00924421.
PIR	A26376.
UniGene	Bt.62430.
3D structure databases
ProteinModelPortal	P12820.
SMR	P12820. Positions 30-100.
ModBase	Search...
Protein-protein interaction databases
STRING	9913.ENSBTAP00000053314.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
eggNOG	NOG71044.
HOGENOM	HOG000007838.
HOVERGEN	HBG103392.
OrthoDB	EOG495ZQZ.
Enzyme and pathway databases
BioCyc	CATTLE:614289-MONOMER. CATTLE:ENSBTAG00000024950-MONOMER.
Family and domain databases
InterPro	IPR001548. Peptidase_M2. [Graphical view]
PANTHER	PTHR10514. PTHR10514. 1 hit.
Pfam	PF01401. Peptidase_M2. 1 hit. [Graphical view]
PROSITE	PS00142. ZINC_PROTEASE. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACE_BOVIN

Accession

Primary (citable) accession number: P12820
Secondary accession number(s): Q0GA41

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	September 2, 2008
Last modified:	May 29, 2013
This is version 83 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program