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P12813

- NR4A1_MOUSE

UniProt

P12813 - NR4A1_MOUSE

Protein

Nuclear receptor subfamily 4 group A member 1

Gene

Nr4a1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 144 (01 Oct 2014)
      Sequence version 1 (01 Oct 1989)
      Previous versions | rss
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    Functioni

    Orphan nuclear receptor. May act concomitantly with NURR1 in regulating the expression of delayed-early genes during liver regeneration. Binds the NGFI-B response element (NBRE) 5'-AAAAGGTCA-3'. May inhibit NF-kappa-B transactivation of IL2. Participates in energy homeostasis by sequestrating the kinase STK11 in the nucleus, thereby attenuating cytoplasmic AMPK activation By similarity.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi267 – 34276Nuclear receptorPROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri270 – 29021NR C4-typePROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri306 – 33025NR C4-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: BHF-UCL
    2. ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity Source: InterPro
    3. protein binding Source: MGI
    4. protein heterodimerization activity Source: MGI
    5. protein homodimerization activity Source: MGI
    6. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: MGI
    7. sequence-specific DNA binding Source: MGI
    8. steroid hormone receptor activity Source: InterPro
    9. zinc ion binding Source: InterPro

    GO - Biological processi

    1. apoptotic process Source: MGI
    2. cell migration involved in sprouting angiogenesis Source: Ensembl
    3. cellular response to fibroblast growth factor stimulus Source: Ensembl
    4. cellular response to organic substance Source: MGI
    5. cellular response to vascular endothelial growth factor stimulus Source: Ensembl
    6. endothelial cell chemotaxis Source: Ensembl
    7. negative regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: MGI
    8. positive regulation of apoptotic process Source: MGI
    9. positive regulation of endothelial cell proliferation Source: Ensembl
    10. positive regulation of transcription, DNA-templated Source: MGI
    11. positive regulation of transcription from RNA polymerase II promoter Source: MGI
    12. response to inorganic substance Source: MGI
    13. skeletal muscle cell differentiation Source: MGI

    Keywords - Molecular functioni

    Receptor

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding, Metal-binding, Zinc

    Enzyme and pathway databases

    ReactomeiREACT_196588. Constitutive PI3K/AKT Signaling in Cancer.
    REACT_218211. AKT phosphorylates targets in the nucleus.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nuclear receptor subfamily 4 group A member 1
    Alternative name(s):
    Nuclear hormone receptor NUR/77
    Nuclear protein N10
    Orphan nuclear receptor HMR
    Gene namesi
    Name:Nr4a1
    Synonyms:Gfrp, Hmr, N10, Nur77
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 15

    Organism-specific databases

    MGIiMGI:1352454. Nr4a1.

    Subcellular locationi

    Cytoplasm By similarity. Nucleus

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nucleus Source: BHF-UCL
    3. transcription factor complex Source: MGI

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 601601Nuclear receptor subfamily 4 group A member 1PRO_0000053716Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei344 – 3441Phosphoserine; by PKABy similarity
    Modified residuei354 – 3541Phosphoserine; by PKA, RPS6KA1 and RPS6KA3By similarity

    Post-translational modificationi

    Phosphorylated at Ser-354 by RPS6KA1 and RPS6KA3 in response to mitogenic or stress stimuli.By similarity
    Acetylated by p300/CBP, acetylation increases stability. Deacetylated by HDAC1 By similarity.By similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    PRIDEiP12813.

    PTM databases

    PhosphoSiteiP12813.

    Expressioni

    Tissue specificityi

    Highest levels occur in the testis followed by brain and muscle tissue.

    Developmental stagei

    Highest levels are found in the adult with barely detectable levels in pre- and early postnatal tissue.

    Inductioni

    By serum growth factors and during liver regeneration.

    Gene expression databases

    ArrayExpressiP12813.
    BgeeiP12813.
    CleanExiMM_NR4A1.
    GenevestigatoriP12813.

    Interactioni

    Subunit structurei

    Interacts with GADD45GIP1. Interacts with STK11. Binds DNA as a monomer By similarity.By similarity

    Protein-protein interaction databases

    BioGridi200346. 4 interactions.
    STRINGi10090.ENSMUSP00000023779.

    Structurei

    3D structure databases

    ProteinModelPortaliP12813.
    SMRiP12813. Positions 268-599.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni412 – 46251Ligand-bindingSequence AnalysisAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi83 – 9412Poly-SerAdd
    BLAST
    Compositional biasi186 – 1905Poly-Pro
    Compositional biasi586 – 5894Poly-Pro

    Sequence similaritiesi

    Contains 1 nuclear receptor DNA-binding domain.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri270 – 29021NR C4-typePROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri306 – 33025NR C4-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiNOG245120.
    HOGENOMiHOG000230925.
    HOVERGENiHBG052663.
    InParanoidiP12813.
    KOiK04465.
    OMAiYSKFQEL.
    OrthoDBiEOG75XGKJ.
    PhylomeDBiP12813.
    TreeFamiTF315430.

    Family and domain databases

    Gene3Di1.10.565.10. 1 hit.
    3.30.50.10. 1 hit.
    InterProiIPR003071. Nuc_orp_HMR_rcpt.
    IPR003070. Nuc_orph_rcpt.
    IPR008946. Nucl_hormone_rcpt_ligand-bd.
    IPR000536. Nucl_hrmn_rcpt_lig-bd_core.
    IPR001723. Str_hrmn_rcpt.
    IPR001628. Znf_hrmn_rcpt.
    IPR013088. Znf_NHR/GATA.
    [Graphical view]
    PfamiPF00104. Hormone_recep. 1 hit.
    PF00105. zf-C4. 1 hit.
    [Graphical view]
    PRINTSiPR01285. HMRNUCRECPTR.
    PR01284. NUCLEARECPTR.
    PR00398. STRDHORMONER.
    PR00047. STROIDFINGER.
    SMARTiSM00430. HOLI. 1 hit.
    SM00399. ZnF_C4. 1 hit.
    [Graphical view]
    SUPFAMiSSF48508. SSF48508. 1 hit.
    PROSITEiPS00031. NUCLEAR_REC_DBD_1. 1 hit.
    PS51030. NUCLEAR_REC_DBD_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P12813-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPCIQAQYGT PATSPGPRDH LTGDPLALEF GKPTMDLASP ETAPAAPATL    50
    PSFSTFMDGY TGEFDTFLYQ LPGTTQPCSS ACSSASSTSS SSSSATSPAS 100
    ASFKFEDFQV YGCYPGTLSG PLDETLSSSG SEYYGSPCSA PSPSTPNFQP 150
    SQLSPWDGSF GHFSPSQTYE GLWAWTEQLP KASSGPPPPP TFFSFSPPTG 200
    PSPSLAQSSL KLFPPPATHQ LGEGESYSMP AAFPGLAPTS PNRDTSGILD 250
    APVTSTKSRS GASGGSEGRC AVCGDNASCQ HYGVRTCEGC KGFFKRTVQK 300
    SAKYICLANK DCPVDKRRRN RCQFCRFQKC LAVGMVKEVV RTDSLKGRRG 350
    RLPSKPKQPP DASPTNLLTS LIRAHLDSGP STAKLDYSKF QELVLPRFGK 400
    EDAGDVQQFY DLLSGSLDVI RKWAEKIPGF IELCPGDQDL LLESAFLELF 450
    ILRLAYRSKP GEGKLIFCSG LVLHQLQCAR GFGDWIDNIL AFSRSLHSLG 500
    VDVPAFACLS ALVLITDRHG LQDPRRVEEL QNRIASCLKE HMATVAGDPQ 550
    PASCLSRLLG KLPELRTLCT QGLQRIFCLK LEDLVPPPPI VDKIFMDTLS 600
    F 601
    Length:601
    Mass (Da):64,738
    Last modified:October 1, 1989 - v1
    Checksum:i8CFF45B58253887B
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti45 – 451A → T in CAA42718. (PubMed:2555161)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J04113 mRNA. Translation: AAA39843.1.
    X16995 Genomic DNA. Translation: CAA34862.1.
    X60132 mRNA. Translation: CAA42718.1.
    BC004770 mRNA. Translation: AAH04770.1.
    CCDSiCCDS27848.1.
    PIRiS06953. QRMSN1.
    RefSeqiNP_034574.1. NM_010444.2.
    XP_006520519.1. XM_006520456.1.
    XP_006520520.1. XM_006520457.1.
    XP_006520521.1. XM_006520458.1.
    XP_006520522.1. XM_006520459.1.
    UniGeneiMm.119.

    Genome annotation databases

    EnsembliENSMUST00000023779; ENSMUSP00000023779; ENSMUSG00000023034.
    GeneIDi15370.
    KEGGimmu:15370.
    UCSCiuc007xsv.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J04113 mRNA. Translation: AAA39843.1 .
    X16995 Genomic DNA. Translation: CAA34862.1 .
    X60132 mRNA. Translation: CAA42718.1 .
    BC004770 mRNA. Translation: AAH04770.1 .
    CCDSi CCDS27848.1.
    PIRi S06953. QRMSN1.
    RefSeqi NP_034574.1. NM_010444.2.
    XP_006520519.1. XM_006520456.1.
    XP_006520520.1. XM_006520457.1.
    XP_006520521.1. XM_006520458.1.
    XP_006520522.1. XM_006520459.1.
    UniGenei Mm.119.

    3D structure databases

    ProteinModelPortali P12813.
    SMRi P12813. Positions 268-599.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 200346. 4 interactions.
    STRINGi 10090.ENSMUSP00000023779.

    PTM databases

    PhosphoSitei P12813.

    Proteomic databases

    PRIDEi P12813.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000023779 ; ENSMUSP00000023779 ; ENSMUSG00000023034 .
    GeneIDi 15370.
    KEGGi mmu:15370.
    UCSCi uc007xsv.2. mouse.

    Organism-specific databases

    CTDi 3164.
    MGIi MGI:1352454. Nr4a1.

    Phylogenomic databases

    eggNOGi NOG245120.
    HOGENOMi HOG000230925.
    HOVERGENi HBG052663.
    InParanoidi P12813.
    KOi K04465.
    OMAi YSKFQEL.
    OrthoDBi EOG75XGKJ.
    PhylomeDBi P12813.
    TreeFami TF315430.

    Enzyme and pathway databases

    Reactomei REACT_196588. Constitutive PI3K/AKT Signaling in Cancer.
    REACT_218211. AKT phosphorylates targets in the nucleus.

    Miscellaneous databases

    NextBioi 288006.
    PROi P12813.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P12813.
    Bgeei P12813.
    CleanExi MM_NR4A1.
    Genevestigatori P12813.

    Family and domain databases

    Gene3Di 1.10.565.10. 1 hit.
    3.30.50.10. 1 hit.
    InterProi IPR003071. Nuc_orp_HMR_rcpt.
    IPR003070. Nuc_orph_rcpt.
    IPR008946. Nucl_hormone_rcpt_ligand-bd.
    IPR000536. Nucl_hrmn_rcpt_lig-bd_core.
    IPR001723. Str_hrmn_rcpt.
    IPR001628. Znf_hrmn_rcpt.
    IPR013088. Znf_NHR/GATA.
    [Graphical view ]
    Pfami PF00104. Hormone_recep. 1 hit.
    PF00105. zf-C4. 1 hit.
    [Graphical view ]
    PRINTSi PR01285. HMRNUCRECPTR.
    PR01284. NUCLEARECPTR.
    PR00398. STRDHORMONER.
    PR00047. STROIDFINGER.
    SMARTi SM00430. HOLI. 1 hit.
    SM00399. ZnF_C4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48508. SSF48508. 1 hit.
    PROSITEi PS00031. NUCLEAR_REC_DBD_1. 1 hit.
    PS51030. NUCLEAR_REC_DBD_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A gene inducible by serum growth factors encodes a member of the steroid and thyroid hormone receptor superfamily."
      Hazel T.G., Nathans D., Lau L.F.
      Proc. Natl. Acad. Sci. U.S.A. 85:8444-8448(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Structure, mapping and expression of a growth factor inducible gene encoding a putative nuclear hormonal binding receptor."
      Ryseck R.P., Macdonald-Bravo H., Mattei M.-G., Ruppert S., Bravo R.
      EMBO J. 8:3327-3335(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Tissue: Kidney and Liver.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Mammary gland.

    Entry informationi

    Entry nameiNR4A1_MOUSE
    AccessioniPrimary (citable) accession number: P12813
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1989
    Last sequence update: October 1, 1989
    Last modified: October 1, 2014
    This is version 144 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3