Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P12813 (NR4A1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 142. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nuclear receptor subfamily 4 group A member 1
Alternative name(s):
Nuclear hormone receptor NUR/77
Nuclear protein N10
Orphan nuclear receptor HMR
Gene names
Name:Nr4a1
Synonyms:Gfrp, Hmr, N10, Nur77
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length601 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Orphan nuclear receptor. May act concomitantly with NURR1 in regulating the expression of delayed-early genes during liver regeneration. Binds the NGFI-B response element (NBRE) 5'-AAAAGGTCA-3'. May inhibit NF-kappa-B transactivation of IL2. Participates in energy homeostasis by sequestrating the kinase STK11 in the nucleus, thereby attenuating cytoplasmic AMPK activation By similarity.

Subunit structure

Interacts with GADD45GIP1. Interacts with STK11. Binds DNA as a monomer By similarity.

Subcellular location

Cytoplasm By similarity. Nucleus.

Tissue specificity

Highest levels occur in the testis followed by brain and muscle tissue.

Developmental stage

Highest levels are found in the adult with barely detectable levels in pre- and early postnatal tissue.

Induction

By serum growth factors and during liver regeneration.

Post-translational modification

Phosphorylated at Ser-354 by RPS6KA1 and RPS6KA3 in response to mitogenic or stress stimuli By similarity.

Acetylated by p300/CBP, acetylation increases stability. Deacetylated by HDAC1 By similarity.

Sequence similarities

Belongs to the nuclear hormone receptor family. NR4 subfamily.

Contains 1 nuclear receptor DNA-binding domain.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentCytoplasm
Nucleus
   DomainZinc-finger
   LigandDNA-binding
Metal-binding
Zinc
   Molecular functionReceptor
   PTMAcetylation
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processapoptotic process

Inferred from mutant phenotype PubMed 17434920. Source: MGI

cell migration involved in sprouting angiogenesis

Inferred from electronic annotation. Source: Ensembl

cellular response to fibroblast growth factor stimulus

Inferred from electronic annotation. Source: Ensembl

cellular response to organic substance

Inferred from direct assay PubMed 21357543. Source: MGI

cellular response to vascular endothelial growth factor stimulus

Inferred from electronic annotation. Source: Ensembl

endothelial cell chemotaxis

Inferred from electronic annotation. Source: Ensembl

negative regulation of cysteine-type endopeptidase activity involved in apoptotic process

Inferred from direct assay PubMed 12815108. Source: MGI

positive regulation of apoptotic process

Inferred from direct assay PubMed 14657025. Source: MGI

positive regulation of endothelial cell proliferation

Inferred from electronic annotation. Source: Ensembl

positive regulation of transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 15155786PubMed 8961274PubMed 9155013. Source: MGI

positive regulation of transcription, DNA-templated

Inferred from direct assay PubMed 14657025PubMed 8961274. Source: MGI

response to inorganic substance

Inferred from sequence alignment PubMed 1491694. Source: MGI

skeletal muscle cell differentiation

Inferred from mutant phenotype PubMed 22147266. Source: MGI

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

nucleus

Inferred from direct assay PubMed 18292087. Source: BHF-UCL

transcription factor complex

Inferred from direct assay PubMed 9155013. Source: MGI

   Molecular_functionDNA binding

Inferred from direct assay PubMed 18292087. Source: BHF-UCL

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription

Inferred from direct assay PubMed 9155013. Source: MGI

ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity

Inferred from electronic annotation. Source: InterPro

protein binding

Inferred from physical interaction PubMed 15155786PubMed 15701640. Source: MGI

protein heterodimerization activity

Inferred from physical interaction PubMed 8961274. Source: MGI

protein homodimerization activity

Inferred from direct assay PubMed 18388149. Source: MGI

sequence-specific DNA binding

Inferred from direct assay PubMed 18388149PubMed 8961274. Source: MGI

steroid hormone receptor activity

Inferred from electronic annotation. Source: InterPro

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 601601Nuclear receptor subfamily 4 group A member 1
PRO_0000053716

Regions

DNA binding267 – 34276Nuclear receptor
Zinc finger270 – 29021NR C4-type
Zinc finger306 – 33025NR C4-type
Region412 – 46251Ligand-binding Potential
Compositional bias83 – 9412Poly-Ser
Compositional bias186 – 1905Poly-Pro
Compositional bias586 – 5894Poly-Pro

Amino acid modifications

Modified residue3441Phosphoserine; by PKA By similarity
Modified residue3541Phosphoserine; by PKA, RPS6KA1 and RPS6KA3 By similarity

Experimental info

Sequence conflict451A → T in CAA42718. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P12813 [UniParc].

Last modified October 1, 1989. Version 1.
Checksum: 8CFF45B58253887B

FASTA60164,738
        10         20         30         40         50         60 
MPCIQAQYGT PATSPGPRDH LTGDPLALEF GKPTMDLASP ETAPAAPATL PSFSTFMDGY 

        70         80         90        100        110        120 
TGEFDTFLYQ LPGTTQPCSS ACSSASSTSS SSSSATSPAS ASFKFEDFQV YGCYPGTLSG 

       130        140        150        160        170        180 
PLDETLSSSG SEYYGSPCSA PSPSTPNFQP SQLSPWDGSF GHFSPSQTYE GLWAWTEQLP 

       190        200        210        220        230        240 
KASSGPPPPP TFFSFSPPTG PSPSLAQSSL KLFPPPATHQ LGEGESYSMP AAFPGLAPTS 

       250        260        270        280        290        300 
PNRDTSGILD APVTSTKSRS GASGGSEGRC AVCGDNASCQ HYGVRTCEGC KGFFKRTVQK 

       310        320        330        340        350        360 
SAKYICLANK DCPVDKRRRN RCQFCRFQKC LAVGMVKEVV RTDSLKGRRG RLPSKPKQPP 

       370        380        390        400        410        420 
DASPTNLLTS LIRAHLDSGP STAKLDYSKF QELVLPRFGK EDAGDVQQFY DLLSGSLDVI 

       430        440        450        460        470        480 
RKWAEKIPGF IELCPGDQDL LLESAFLELF ILRLAYRSKP GEGKLIFCSG LVLHQLQCAR 

       490        500        510        520        530        540 
GFGDWIDNIL AFSRSLHSLG VDVPAFACLS ALVLITDRHG LQDPRRVEEL QNRIASCLKE 

       550        560        570        580        590        600 
HMATVAGDPQ PASCLSRLLG KLPELRTLCT QGLQRIFCLK LEDLVPPPPI VDKIFMDTLS 


F 

« Hide

References

« Hide 'large scale' references
[1]"A gene inducible by serum growth factors encodes a member of the steroid and thyroid hormone receptor superfamily."
Hazel T.G., Nathans D., Lau L.F.
Proc. Natl. Acad. Sci. U.S.A. 85:8444-8448(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Structure, mapping and expression of a growth factor inducible gene encoding a putative nuclear hormonal binding receptor."
Ryseck R.P., Macdonald-Bravo H., Mattei M.-G., Ruppert S., Bravo R.
EMBO J. 8:3327-3335(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Tissue: Kidney and Liver.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Mammary gland.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
J04113 mRNA. Translation: AAA39843.1.
X16995 Genomic DNA. Translation: CAA34862.1.
X60132 mRNA. Translation: CAA42718.1.
BC004770 mRNA. Translation: AAH04770.1.
CCDSCCDS27848.1.
PIRQRMSN1. S06953.
RefSeqNP_034574.1. NM_010444.2.
XP_006520519.1. XM_006520456.1.
XP_006520520.1. XM_006520457.1.
XP_006520521.1. XM_006520458.1.
XP_006520522.1. XM_006520459.1.
UniGeneMm.119.

3D structure databases

ProteinModelPortalP12813.
SMRP12813. Positions 268-599.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid200346. 4 interactions.
STRING10090.ENSMUSP00000023779.

PTM databases

PhosphoSiteP12813.

Proteomic databases

PRIDEP12813.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000023779; ENSMUSP00000023779; ENSMUSG00000023034.
GeneID15370.
KEGGmmu:15370.
UCSCuc007xsv.2. mouse.

Organism-specific databases

CTD3164.
MGIMGI:1352454. Nr4a1.

Phylogenomic databases

eggNOGNOG245120.
HOGENOMHOG000230925.
HOVERGENHBG052663.
InParanoidP12813.
KOK04465.
OMAYSKFQEL.
OrthoDBEOG75XGKJ.
PhylomeDBP12813.
TreeFamTF315430.

Gene expression databases

ArrayExpressP12813.
BgeeP12813.
CleanExMM_NR4A1.
GenevestigatorP12813.

Family and domain databases

Gene3D1.10.565.10. 1 hit.
3.30.50.10. 1 hit.
InterProIPR003071. Nuc_orp_HMR_rcpt.
IPR003070. Nuc_orph_rcpt.
IPR008946. Nucl_hormone_rcpt_ligand-bd.
IPR000536. Nucl_hrmn_rcpt_lig-bd_core.
IPR001723. Str_hrmn_rcpt.
IPR001628. Znf_hrmn_rcpt.
IPR013088. Znf_NHR/GATA.
[Graphical view]
PfamPF00104. Hormone_recep. 1 hit.
PF00105. zf-C4. 1 hit.
[Graphical view]
PRINTSPR01285. HMRNUCRECPTR.
PR01284. NUCLEARECPTR.
PR00398. STRDHORMONER.
PR00047. STROIDFINGER.
SMARTSM00430. HOLI. 1 hit.
SM00399. ZnF_C4. 1 hit.
[Graphical view]
SUPFAMSSF48508. SSF48508. 1 hit.
PROSITEPS00031. NUCLEAR_REC_DBD_1. 1 hit.
PS51030. NUCLEAR_REC_DBD_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio288006.
PROP12813.
SOURCESearch...

Entry information

Entry nameNR4A1_MOUSE
AccessionPrimary (citable) accession number: P12813
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 1, 1989
Last modified: July 9, 2014
This is version 142 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot