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P12798

- KPBB_RABIT

UniProt

P12798 - KPBB_RABIT

Protein

Phosphorylase b kinase regulatory subunit beta

Gene

PHKB

Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 113 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Phosphorylase b kinase catalyzes the phosphorylation of serine in certain substrates, including troponin I. The beta chain acts as a regulatory unit and modulates the activity of the holoenzyme in response to phosphorylation.

    Enzyme regulationi

    By phosphorylation of various serine residues.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei1090 – 10901Not methylated

    GO - Molecular functioni

    1. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro

    GO - Biological processi

    1. glycogen metabolic process Source: UniProtKB-UniPathway

    Keywords - Biological processi

    Carbohydrate metabolism, Glycogen metabolism

    Keywords - Ligandi

    Calmodulin-binding

    Enzyme and pathway databases

    UniPathwayiUPA00163.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphorylase b kinase regulatory subunit beta
    Short name:
    Phosphorylase kinase subunit beta
    Gene namesi
    Name:PHKB
    OrganismiOryctolagus cuniculus (Rabbit)
    Taxonomic identifieri9986 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
    ProteomesiUP000001811: Unplaced

    Subcellular locationi

    Cell membrane Curated; Lipid-anchor Curated; Cytoplasmic side Curated

    GO - Cellular componenti

    1. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 10931092Phosphorylase b kinase regulatory subunit betaPRO_0000057738Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanine1 Publication
    Modified residuei12 – 121Phosphoserine; by autocatalysis2 Publications
    Modified residuei27 – 271Phosphoserine; by PKA2 Publications
    Modified residuei701 – 7011Phosphoserine; by PKA2 Publications
    Lipidationi1090 – 10901S-farnesyl cysteine1 Publication

    Post-translational modificationi

    Cys-1090 is farnesylated, but the terminal tripeptide is not removed and the cysteine carboxyl is not methylated.

    Keywords - PTMi

    Acetylation, Lipoprotein, Phosphoprotein, Prenylation

    Interactioni

    Subunit structurei

    Hexadecamer of 4 heterotetramers, each composed of alpha, beta, gamma, and delta subunits. Alpha (PHKA1 or PHKA2) and beta (PHKB) are regulatory subunits, gamma (PHKG1 or PHKG2) is the catalytic subunit, and delta is calmodulin.

    Protein-protein interaction databases

    DIPiDIP-48333N.
    IntActiP12798. 1 interaction.
    MINTiMINT-8146705.
    STRINGi9986.ENSOCUP00000013584.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni7 – 2923Calmodulin-bindingSequence AnalysisAdd
    BLAST
    Regioni768 – 79528Calmodulin-bindingSequence AnalysisAdd
    BLAST
    Regioni920 – 95132Calmodulin-bindingSequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiNOG82518.
    HOGENOMiHOG000231477.
    HOVERGENiHBG097309.

    Family and domain databases

    InterProiIPR008928. 6-hairpin_glycosidase-like.
    IPR011613. Glyco_hydro_15.
    IPR008734. PHK_A/B_su.
    [Graphical view]
    PANTHERiPTHR10749. PTHR10749. 1 hit.
    PfamiPF00723. Glyco_hydro_15. 1 hit.
    [Graphical view]
    SUPFAMiSSF48208. SSF48208. 1 hit.

    Sequences (4)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 4 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P12798-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAGATGLMAE VSWKVLERRA RTKRSGSVYE PLKSINLPRP DNETLWDKLD     50
    YYYKIVKSTL LLYQSPTTGL FPTKTCGGDQ TAKIHDSLYC AAGAWALALA 100
    YRRIDDDKGR THELEHSAIK CMRGILYCYM RQADKVQQFK QDPRPTTCLH 150
    SLFNVHTGDE LLSYEEYGHL QINAVSLYLL YLVEMISSGL QIIYNTDEVS 200
    FIQNLVFCVE RVYRVPDFGV WERGSKYNNG STELHSSSVG LAKAALEAIN 250
    GFNLFGNQGC SWSVIFVDLD AHNRNRQTLC SLLPRESRSH NTDAALLPCI 300
    SYPAFALDDD VLYNQTLDKV IRKLKGKYGF KRFLRDGYRT SLEDPKRRYY 350
    KPAEIKLFDG IECEFPIFFL YMMIDGVFRG NPKQVKEYQD LLTPVLHQTT 400
    EGYPVVPKYY YVPADFVEYE KRNPGSQKRF PSNCGRDGKL FLWGQALYII 450
    AKLLADELIS PKDIDPVQRY VPLQNQRNVS MRYSNQGPLE NDLVVHVALV 500
    AESQRLQVFL NTYGIQTQTP QQVEPIQIWP QQELVKAYFH LGINEKLGLS 550
    GRPDRPIGCL GTSKIYRILG KTVVCYPIIF DLSDFYMSQD VLLLIDDIKN 600
    ALQFIKQYWK MHGRPLFLVL IREDNIRGSR FNPMLDMLAA LKNGMIGGVK 650
    VHVDRLQTLI SGAVVEQLDF LRISDTEELP EFKSFEELEP PKHSKVKRQS 700
    STSNAPELEQ QPEVSVTEWR NKPTHEILQK LNDCSCLASQ TILLGILLKR 750
    EGPNFITQEG TVSDHIERLY RRAGSKKLWL AVRYGAAFTQ KFSSSIAPHI 800
    TTFLVHGKQV TLGAFGHEEE VISNPLSPRV IKNIIYYKCN THDEREAVIQ 850
    QELVIHIGWI ISNNPELFSG MLKIRIGWII HAMEYELQIR SGDKPAKDLY 900
    QLSPSEVKQL LLDILQPQQN GRCWLNKRQI DGSLNRTPTG FYDRVWQILE 950
    RTPNGIIVAG KHLPQQPTLS DMTMYEMNFS LLVEDMLGNI DQPKYRQIVV 1000
    ELLMVVSIVL ERNPELEFQD KVDLDKLVKE AFHEFQKDES RLKEIEKQDD 1050
    MTSFYNTPPL GKRGTCSYLT KVVMNLLLEG EVKPSNEDSC LVS 1093
    Length:1,093
    Mass (Da):125,295
    Last modified:January 23, 2007 - v3
    Checksum:i5954A72A50CD6F3C
    GO
    Isoform 2 (identifier: P12798-2) [UniParc]FASTAAdd to Basket

    Also known as: Brain

    The sequence of this isoform differs from the canonical sequence as follows:
         1-23: MAGATGLMAEVSWKVLERRARTK → MASSADAVVSSPPAFL

    Show »
    Length:1,086
    Mass (Da):124,283
    Checksum:i5163CA948AF0479B
    GO
    Isoform 3 (identifier: P12798-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         780-806: LAVRYGAAFTQKFSSSIAPHITTFLVH → SVVRRAASLLNKVVDSLAPSITNVLVQ

    Show »
    Length:1,093
    Mass (Da):125,182
    Checksum:i07F26602E742B485
    GO
    Isoform 4 (identifier: P12798-4) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-23: MAGATGLMAEVSWKVLERRARTK → MASSADAVVSSPPAFL
         780-806: LAVRYGAAFTQKFSSSIAPHITTFLVH → SVVRRAASLLNKVVDSLAPSITNVLVQ

    Show »
    Length:1,086
    Mass (Da):124,170
    Checksum:i0FC50BBC3D7F9C22
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti24 – 241R → Q AA sequence (PubMed:164350)Curated
    Sequence conflicti30 – 301Missing AA sequence (PubMed:164350)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti28 – 281V → I.

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 2323MAGAT…RARTK → MASSADAVVSSPPAFL in isoform 2 and isoform 4. CuratedVSP_004702Add
    BLAST
    Alternative sequencei780 – 80627LAVRY…TFLVH → SVVRRAASLLNKVVDSLAPS ITNVLVQ in isoform 3 and isoform 4. CuratedVSP_004703Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J04120 mRNA. Translation: AAA31447.1.
    M64657 mRNA. Translation: AAA31450.1.
    M64658 mRNA. Translation: AAA31448.1.
    PIRiA31758.
    B40793.
    RefSeqiNP_001075770.1. NM_001082301.1. [P12798-4]
    UniGeneiOcu.1949.

    Genome annotation databases

    GeneIDi100009137.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J04120 mRNA. Translation: AAA31447.1 .
    M64657 mRNA. Translation: AAA31450.1 .
    M64658 mRNA. Translation: AAA31448.1 .
    PIRi A31758.
    B40793.
    RefSeqi NP_001075770.1. NM_001082301.1. [P12798-4 ]
    UniGenei Ocu.1949.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-48333N.
    IntActi P12798. 1 interaction.
    MINTi MINT-8146705.
    STRINGi 9986.ENSOCUP00000013584.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 100009137.

    Organism-specific databases

    CTDi 5257.

    Phylogenomic databases

    eggNOGi NOG82518.
    HOGENOMi HOG000231477.
    HOVERGENi HBG097309.

    Enzyme and pathway databases

    UniPathwayi UPA00163 .

    Family and domain databases

    InterProi IPR008928. 6-hairpin_glycosidase-like.
    IPR011613. Glyco_hydro_15.
    IPR008734. PHK_A/B_su.
    [Graphical view ]
    PANTHERi PTHR10749. PTHR10749. 1 hit.
    Pfami PF00723. Glyco_hydro_15. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48208. SSF48208. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The alpha and beta subunits of phosphorylase kinase are homologous: cDNA cloning and primary structure of the beta subunit."
      Kilimann M.W., Zander N.F., Kuhn C.C., Crabb J.W., Meyer H.E., Heilmeyer L.M.G. Jr.
      Proc. Natl. Acad. Sci. U.S.A. 85:9381-9385(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
      Tissue: Skeletal muscle.
    2. "Isoform diversity of phosphorylase kinase alpha and beta subunits generated by alternative RNA splicing."
      Harmann B., Zander N.F., Kilimann M.W.
      J. Biol. Chem. 266:15631-15637(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALTERNATIVE SPLICING.
    3. "Localization of phosphoserine residues in the alpha subunit of rabbit skeletal muscle phosphorylase kinase."
      Meyer H.E., Meyer G.F., Dirks H., Heilmeyer L.M.G. Jr.
      Eur. J. Biochem. 188:367-376(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-18; 25-33; 688-709 AND 1072-1093, PHOSPHORYLATION AT SER-12; SER-27 AND SER-701.
    4. "Electrophoretic purification of the alpha and beta subunits of phosphorylase kinase and evidence in support of the deduced amino acid sequences."
      Crabb J.W., Harris W.R., Johnson C.M., Sotiroudis T.G., Kuhn C.C., Heilmeyer L.M. Jr.
      Electrophoresis 11:133-140(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 34-1081.
    5. "The hormonal control of activity of skeletal muscle phosphorylase kinase. Amino-acid sequences at the two sites of action of adenosine-3':5'-monophosphate-dependent protein kinase."
      Cohen P., Watson D.C., Dixon G.H.
      Eur. J. Biochem. 51:79-92(1975) [PubMed] [Europe PMC] [Abstract]
      Cited for: PARTIAL PROTEIN SEQUENCE.
      Tissue: Skeletal muscle.
    6. "Farnesylcysteine, a constituent of the alpha and beta subunits of rabbit skeletal muscle phosphorylase kinase: localization by conversion to S-ethylcysteine and by tandem mass spectrometry."
      Heilmeyer L.M. Jr., Serwe M., Weber C., Metzger J., Hoffmann-Posorske E., Meyer H.E.
      Proc. Natl. Acad. Sci. U.S.A. 89:9554-9558(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1073-1093, ISOPRENYLATION AT CYS-1090, IDENTIFICATION BY MASS SPECTROMETRY.

    Entry informationi

    Entry nameiKPBB_RABIT
    AccessioniPrimary (citable) accession number: P12798
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1989
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 113 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3