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Protein

Phosphorylase b kinase regulatory subunit beta

Gene

PHKB

Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Phosphorylase b kinase catalyzes the phosphorylation of serine in certain substrates, including troponin I. The beta chain acts as a regulatory unit and modulates the activity of the holoenzyme in response to phosphorylation.

Enzyme regulationi

By phosphorylation of various serine residues.

Pathwayi: glycogen metabolism

This protein is involved in the pathway glycogen metabolism, which is part of Glycan biosynthesis.
View all proteins of this organism that are known to be involved in the pathway glycogen metabolism and in Glycan biosynthesis.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionCalmodulin-binding
Biological processCarbohydrate metabolism, Glycogen metabolism

Enzyme and pathway databases

BRENDAi2.7.11.19 1749
UniPathwayiUPA00163

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphorylase b kinase regulatory subunit beta
Short name:
Phosphorylase kinase subunit beta
Gene namesi
Name:PHKB
OrganismiOryctolagus cuniculus (Rabbit)
Taxonomic identifieri9986 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
Proteomesi
  • UP000001811 Componenti: Unplaced

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00000577382 – 1093Phosphorylase b kinase regulatory subunit betaAdd BLAST1092

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanine1 Publication1
Modified residuei12Phosphoserine; by autocatalysis2 Publications1
Modified residuei27Phosphoserine; by PKA2 Publications1
Modified residuei701Phosphoserine; by PKA2 Publications1
Lipidationi1090S-farnesyl cysteine1 Publication1

Post-translational modificationi

Cys-1090 is farnesylated, but the C-terminal tripeptide is not removed and the cysteine carboxyl is not methylated.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei1090Not methylated1

Keywords - PTMi

Acetylation, Lipoprotein, Phosphoprotein, Prenylation

Proteomic databases

PRIDEiP12798

PTM databases

iPTMnetiP12798

Interactioni

Subunit structurei

Hexadecamer of 4 heterotetramers, each composed of alpha, beta, gamma, and delta subunits. Alpha (PHKA1 or PHKA2) and beta (PHKB) are regulatory subunits, gamma (PHKG1 or PHKG2) is the catalytic subunit, and delta is calmodulin.

GO - Molecular functioni

Protein-protein interaction databases

DIPiDIP-48333N
IntActiP12798, 2 interactors
MINTiP12798
STRINGi9986.ENSOCUP00000013584

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni7 – 29Calmodulin-bindingSequence analysisAdd BLAST23
Regioni768 – 795Calmodulin-bindingSequence analysisAdd BLAST28
Regioni920 – 951Calmodulin-bindingSequence analysisAdd BLAST32

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3635 Eukaryota
ENOG410XPJZ LUCA
HOGENOMiHOG000231477
HOVERGENiHBG097309
InParanoidiP12798
KOiK07190

Family and domain databases

InterProiView protein in InterPro
IPR008928 6-hairpin_glycosidase_sf
IPR011613 Glyco_hydro_15/PHK
IPR008734 PHK_A/B_su
PANTHERiPTHR10749 PTHR10749, 1 hit
PfamiView protein in Pfam
PF00723 Glyco_hydro_15, 1 hit
SUPFAMiSSF48208 SSF48208, 1 hit

Sequences (4)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P12798-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAGATGLMAE VSWKVLERRA RTKRSGSVYE PLKSINLPRP DNETLWDKLD
60 70 80 90 100
YYYKIVKSTL LLYQSPTTGL FPTKTCGGDQ TAKIHDSLYC AAGAWALALA
110 120 130 140 150
YRRIDDDKGR THELEHSAIK CMRGILYCYM RQADKVQQFK QDPRPTTCLH
160 170 180 190 200
SLFNVHTGDE LLSYEEYGHL QINAVSLYLL YLVEMISSGL QIIYNTDEVS
210 220 230 240 250
FIQNLVFCVE RVYRVPDFGV WERGSKYNNG STELHSSSVG LAKAALEAIN
260 270 280 290 300
GFNLFGNQGC SWSVIFVDLD AHNRNRQTLC SLLPRESRSH NTDAALLPCI
310 320 330 340 350
SYPAFALDDD VLYNQTLDKV IRKLKGKYGF KRFLRDGYRT SLEDPKRRYY
360 370 380 390 400
KPAEIKLFDG IECEFPIFFL YMMIDGVFRG NPKQVKEYQD LLTPVLHQTT
410 420 430 440 450
EGYPVVPKYY YVPADFVEYE KRNPGSQKRF PSNCGRDGKL FLWGQALYII
460 470 480 490 500
AKLLADELIS PKDIDPVQRY VPLQNQRNVS MRYSNQGPLE NDLVVHVALV
510 520 530 540 550
AESQRLQVFL NTYGIQTQTP QQVEPIQIWP QQELVKAYFH LGINEKLGLS
560 570 580 590 600
GRPDRPIGCL GTSKIYRILG KTVVCYPIIF DLSDFYMSQD VLLLIDDIKN
610 620 630 640 650
ALQFIKQYWK MHGRPLFLVL IREDNIRGSR FNPMLDMLAA LKNGMIGGVK
660 670 680 690 700
VHVDRLQTLI SGAVVEQLDF LRISDTEELP EFKSFEELEP PKHSKVKRQS
710 720 730 740 750
STSNAPELEQ QPEVSVTEWR NKPTHEILQK LNDCSCLASQ TILLGILLKR
760 770 780 790 800
EGPNFITQEG TVSDHIERLY RRAGSKKLWL AVRYGAAFTQ KFSSSIAPHI
810 820 830 840 850
TTFLVHGKQV TLGAFGHEEE VISNPLSPRV IKNIIYYKCN THDEREAVIQ
860 870 880 890 900
QELVIHIGWI ISNNPELFSG MLKIRIGWII HAMEYELQIR SGDKPAKDLY
910 920 930 940 950
QLSPSEVKQL LLDILQPQQN GRCWLNKRQI DGSLNRTPTG FYDRVWQILE
960 970 980 990 1000
RTPNGIIVAG KHLPQQPTLS DMTMYEMNFS LLVEDMLGNI DQPKYRQIVV
1010 1020 1030 1040 1050
ELLMVVSIVL ERNPELEFQD KVDLDKLVKE AFHEFQKDES RLKEIEKQDD
1060 1070 1080 1090
MTSFYNTPPL GKRGTCSYLT KVVMNLLLEG EVKPSNEDSC LVS
Length:1,093
Mass (Da):125,295
Last modified:January 23, 2007 - v3
Checksum:i5954A72A50CD6F3C
GO
Isoform 2 (identifier: P12798-2) [UniParc]FASTAAdd to basket
Also known as: Brain

The sequence of this isoform differs from the canonical sequence as follows:
     1-23: MAGATGLMAEVSWKVLERRARTK → MASSADAVVSSPPAFL

Show »
Length:1,086
Mass (Da):124,283
Checksum:i5163CA948AF0479B
GO
Isoform 3 (identifier: P12798-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     780-806: LAVRYGAAFTQKFSSSIAPHITTFLVH → SVVRRAASLLNKVVDSLAPSITNVLVQ

Show »
Length:1,093
Mass (Da):125,182
Checksum:i07F26602E742B485
GO
Isoform 4 (identifier: P12798-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-23: MAGATGLMAEVSWKVLERRARTK → MASSADAVVSSPPAFL
     780-806: LAVRYGAAFTQKFSSSIAPHITTFLVH → SVVRRAASLLNKVVDSLAPSITNVLVQ

Show »
Length:1,086
Mass (Da):124,170
Checksum:i0FC50BBC3D7F9C22
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti24R → Q AA sequence (PubMed:164350).Curated1
Sequence conflicti30Missing AA sequence (PubMed:164350).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti28V → I. 1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0047021 – 23MAGAT…RARTK → MASSADAVVSSPPAFL in isoform 2 and isoform 4. CuratedAdd BLAST23
Alternative sequenceiVSP_004703780 – 806LAVRY…TFLVH → SVVRRAASLLNKVVDSLAPS ITNVLVQ in isoform 3 and isoform 4. CuratedAdd BLAST27

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04120 mRNA Translation: AAA31447.1
M64657 mRNA Translation: AAA31450.1
M64658 mRNA Translation: AAA31448.1
PIRiA31758
B40793
RefSeqiNP_001075770.1, NM_001082301.1 [P12798-4]
UniGeneiOcu.1949

Genome annotation databases

GeneIDi100009137
KEGGiocu:100009137

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiKPBB_RABIT
AccessioniPrimary (citable) accession number: P12798
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: January 23, 2007
Last modified: March 28, 2018
This is version 127 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health