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P12791

- CP2BA_MOUSE

UniProt

P12791 - CP2BA_MOUSE

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Protein

Cytochrome P450 2B10

Gene

Cyp2b10

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.

Catalytic activityi

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactori

Heme group.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi445 – 4451Iron (heme axial ligand)

GO - Molecular functioni

  1. aromatase activity Source: UniProtKB-EC
  2. heme binding Source: InterPro
  3. iron ion binding Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 2B10 (EC:1.14.14.1)
Alternative name(s):
CYPIIB10
CYPIIB20
Cytochrome P450 2B20
Cytochrome P450 clone PF3/46
Cytochrome P450-16-alpha
P24
Testosterone 16-alpha hydroxylase
Gene namesi
Name:Cyp2b10
Synonyms:Cyp2b-10, Cyp2b20
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Unplaced

Organism-specific databases

MGIiMGI:88598. Cyp2b10.

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProtKB-KW
  2. membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 500500Cytochrome P450 2B10PRO_0000051685Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei128 – 1281Phosphoserine; by PKABy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP12791.
PaxDbiP12791.
PRIDEiP12791.

Expressioni

Inductioni

Up-regulated by cannabidiol.1 Publication

Gene expression databases

GenevestigatoriP12791.

Structurei

3D structure databases

ProteinModelPortaliP12791.
SMRiP12791. Positions 28-500.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

eggNOGiCOG2124.
HOGENOMiHOG000036992.
HOVERGENiHBG015789.
InParanoidiP12791.
PhylomeDBiP12791.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR002401. Cyt_P450_E_grp-I.
IPR008068. Cyt_P450_E_grp-I_CYP2B-like.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR01685. EP450ICYP2B.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P12791-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MEPSVLLLLA LLVGFLLLLA RGHPKSRGNF PPGPRPLPLL GNLLQMDRGG
60 70 80 90 100
LLKSLIQLRE KYGDVFTVHL GPRPVVMLCG TDTIREALVG QAEAFSGRGT
110 120 130 140 150
VAVVEPTFKE YGVIFANGER WKTLRRFSLA TMRDFGMGKR SVEERIQEEA
160 170 180 190 200
QCLVEELRKS QGAPLDPTFL FQCITANVIC SIVFGERFEY TDRQFLRLLE
210 220 230 240 250
LFYQTFSLIS SFSSQMFELF SGFLKYFPGA HRQISKNLQE LLDYIGHSVE
260 270 280 290 300
RHKATLDPSV PRDFIDIYLL RMEKEKSNQN AEFHHQNLMM SVLSLFFVGT
310 320 330 340 350
ETSSTTLHYG FLLMLKYPHV TEKVQKEIDQ VIGSHRLPTL DDRTKMPYSD
360 370 380 390 400
AVIHEIQRFS DLIPIGVPHR VTKDTLFRGY LLPKNTEVYP ILSSALHDPQ
410 420 430 440 450
YFEQPDSFNP DQFLDANGAL KKSEAFLPFS TGQIFDQKSV GKRICLGESI
460 470 480 490 500
ARSELFLFFT SILQNFSVAS HVAPKDIDLT PKESGIGKIP PTYQICFLAR
Length:500
Mass (Da):56,744
Last modified:October 1, 1989 - v1
Checksum:iF660A00D8D0FBA94
GO
Isoform 2 (identifier: P12791-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     433-441: Missing.

Show »
Length:491
Mass (Da):55,740
Checksum:iB4C7AAE1435FC7C9
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti55 – 551L → F in CAA68051. (PubMed:8831708)Curated
Sequence conflicti178 – 1781V → I in CAA68051. (PubMed:8831708)Curated
Sequence conflicti251 – 2533RHK → KHR in CAA68051. (PubMed:8831708)Curated
Sequence conflicti280 – 2812NA → HT in CAA68051. (PubMed:8831708)Curated
Sequence conflicti298 – 2981V → A in CAA68051. (PubMed:8831708)Curated
Sequence conflicti308 – 3081H → R in CAA68051. (PubMed:8831708)Curated
Sequence conflicti321 – 3211T → A in CAA68051. (PubMed:8831708)Curated
Sequence conflicti349 – 3491S → T in CAA68051. (PubMed:8831708)Curated
Sequence conflicti376 – 3761L → M in CAA68051. (PubMed:8831708)Curated
Sequence conflicti412 – 4121Q → H in CAA68051. (PubMed:8831708)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei433 – 4419Missing in isoform 2. 1 PublicationVSP_022500

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M21856 mRNA. Translation: AAA40425.1.
X99715 mRNA. Translation: CAA68051.1. Sequence problems.
CCDSiCCDS20999.1. [P12791-2]
PIRiB31047.
UniGeneiMm.218749.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M21856 mRNA. Translation: AAA40425.1 .
X99715 mRNA. Translation: CAA68051.1 . Sequence problems.
CCDSi CCDS20999.1. [P12791-2 ]
PIRi B31047.
UniGenei Mm.218749.

3D structure databases

ProteinModelPortali P12791.
SMRi P12791. Positions 28-500.
ModBasei Search...
MobiDBi Search...

Chemistry

ChEMBLi CHEMBL1907983.

Proteomic databases

MaxQBi P12791.
PaxDbi P12791.
PRIDEi P12791.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Organism-specific databases

MGIi MGI:88598. Cyp2b10.

Phylogenomic databases

eggNOGi COG2124.
HOGENOMi HOG000036992.
HOVERGENi HBG015789.
InParanoidi P12791.
PhylomeDBi P12791.

Miscellaneous databases

ChiTaRSi Cyp2b10. mouse.
PROi P12791.
SOURCEi Search...

Gene expression databases

Genevestigatori P12791.

Family and domain databases

Gene3Di 1.10.630.10. 1 hit.
InterProi IPR001128. Cyt_P450.
IPR002401. Cyt_P450_E_grp-I.
IPR008068. Cyt_P450_E_grp-I_CYP2B-like.
[Graphical view ]
Pfami PF00067. p450. 1 hit.
[Graphical view ]
PRINTSi PR00463. EP450I.
PR01685. EP450ICYP2B.
PR00385. P450.
SUPFAMi SSF48264. SSF48264. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Rip locus: regulation of female-specific isozyme (I-P-450(16 alpha) of testosterone 16 alpha-hydroxylase in mouse liver, chromosome localization, and cloning of P-450 cDNA."
    Noshiro M., Lakso M., Kawajiri K., Negishi M.
    Biochemistry 27:6434-6443(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. "Isolation of a new mouse cDNA clone: hybrid form of cytochrome P450 2b10 and NADPH-cytochrome P450 oxidoreductase."
    Damon M., Fautrel A., Marc N., Guillouzo A., Corcos L.
    Biochem. Biophys. Res. Commun. 226:900-905(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-452 (ISOFORM 2).
    Strain: BALB/c.
    Tissue: Liver.
  3. "Purification and characterization of a mouse liver cytochrome P-450 induced by cannabidiol."
    Bornheim L.M., Correia M.A.
    Mol. Pharmacol. 36:377-383(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-15, INDUCTION.

Entry informationi

Entry nameiCP2BA_MOUSE
AccessioniPrimary (citable) accession number: P12791
Secondary accession number(s): Q62397
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 1, 1989
Last modified: October 29, 2014
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3