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P12791

- CP2BA_MOUSE

UniProt

P12791 - CP2BA_MOUSE

Protein

Cytochrome P450 2B10

Gene

Cyp2b10

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 117 (01 Oct 2014)
      Sequence version 1 (01 Oct 1989)
      Previous versions | rss
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    Functioni

    Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.

    Catalytic activityi

    RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

    Cofactori

    Heme group.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi445 – 4451Iron (heme axial ligand)

    GO - Molecular functioni

    1. aromatase activity Source: UniProtKB-EC
    2. heme binding Source: InterPro
    3. iron ion binding Source: InterPro

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase

    Keywords - Ligandi

    Heme, Iron, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytochrome P450 2B10 (EC:1.14.14.1)
    Alternative name(s):
    CYPIIB10
    CYPIIB20
    Cytochrome P450 2B20
    Cytochrome P450 clone PF3/46
    Cytochrome P450-16-alpha
    P24
    Testosterone 16-alpha hydroxylase
    Gene namesi
    Name:Cyp2b10
    Synonyms:Cyp2b-10, Cyp2b20
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Unplaced

    Organism-specific databases

    MGIiMGI:88598. Cyp2b10.

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Microsome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 500500Cytochrome P450 2B10PRO_0000051685Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei128 – 1281Phosphoserine; by PKABy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP12791.
    PaxDbiP12791.
    PRIDEiP12791.

    Expressioni

    Inductioni

    Up-regulated by cannabidiol.1 Publication

    Gene expression databases

    GenevestigatoriP12791.

    Structurei

    3D structure databases

    ProteinModelPortaliP12791.
    SMRiP12791. Positions 28-500.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytochrome P450 family.Curated

    Phylogenomic databases

    eggNOGiCOG2124.
    HOGENOMiHOG000036992.
    HOVERGENiHBG015789.
    InParanoidiP12791.
    PhylomeDBiP12791.

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiIPR001128. Cyt_P450.
    IPR002401. Cyt_P450_E_grp-I.
    IPR008068. Cyt_P450_E_grp-I_CYP2B-like.
    [Graphical view]
    PfamiPF00067. p450. 1 hit.
    [Graphical view]
    PRINTSiPR00463. EP450I.
    PR01685. EP450ICYP2B.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P12791-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MEPSVLLLLA LLVGFLLLLA RGHPKSRGNF PPGPRPLPLL GNLLQMDRGG    50
    LLKSLIQLRE KYGDVFTVHL GPRPVVMLCG TDTIREALVG QAEAFSGRGT 100
    VAVVEPTFKE YGVIFANGER WKTLRRFSLA TMRDFGMGKR SVEERIQEEA 150
    QCLVEELRKS QGAPLDPTFL FQCITANVIC SIVFGERFEY TDRQFLRLLE 200
    LFYQTFSLIS SFSSQMFELF SGFLKYFPGA HRQISKNLQE LLDYIGHSVE 250
    RHKATLDPSV PRDFIDIYLL RMEKEKSNQN AEFHHQNLMM SVLSLFFVGT 300
    ETSSTTLHYG FLLMLKYPHV TEKVQKEIDQ VIGSHRLPTL DDRTKMPYSD 350
    AVIHEIQRFS DLIPIGVPHR VTKDTLFRGY LLPKNTEVYP ILSSALHDPQ 400
    YFEQPDSFNP DQFLDANGAL KKSEAFLPFS TGQIFDQKSV GKRICLGESI 450
    ARSELFLFFT SILQNFSVAS HVAPKDIDLT PKESGIGKIP PTYQICFLAR 500
    Length:500
    Mass (Da):56,744
    Last modified:October 1, 1989 - v1
    Checksum:iF660A00D8D0FBA94
    GO
    Isoform 2 (identifier: P12791-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         433-441: Missing.

    Show »
    Length:491
    Mass (Da):55,740
    Checksum:iB4C7AAE1435FC7C9
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti55 – 551L → F in CAA68051. (PubMed:8831708)Curated
    Sequence conflicti178 – 1781V → I in CAA68051. (PubMed:8831708)Curated
    Sequence conflicti251 – 2533RHK → KHR in CAA68051. (PubMed:8831708)Curated
    Sequence conflicti280 – 2812NA → HT in CAA68051. (PubMed:8831708)Curated
    Sequence conflicti298 – 2981V → A in CAA68051. (PubMed:8831708)Curated
    Sequence conflicti308 – 3081H → R in CAA68051. (PubMed:8831708)Curated
    Sequence conflicti321 – 3211T → A in CAA68051. (PubMed:8831708)Curated
    Sequence conflicti349 – 3491S → T in CAA68051. (PubMed:8831708)Curated
    Sequence conflicti376 – 3761L → M in CAA68051. (PubMed:8831708)Curated
    Sequence conflicti412 – 4121Q → H in CAA68051. (PubMed:8831708)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei433 – 4419Missing in isoform 2. 1 PublicationVSP_022500

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M21856 mRNA. Translation: AAA40425.1.
    X99715 mRNA. Translation: CAA68051.1. Sequence problems.
    CCDSiCCDS20999.1. [P12791-2]
    PIRiB31047.
    UniGeneiMm.218749.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M21856 mRNA. Translation: AAA40425.1 .
    X99715 mRNA. Translation: CAA68051.1 . Sequence problems.
    CCDSi CCDS20999.1. [P12791-2 ]
    PIRi B31047.
    UniGenei Mm.218749.

    3D structure databases

    ProteinModelPortali P12791.
    SMRi P12791. Positions 28-500.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    ChEMBLi CHEMBL1907983.

    Proteomic databases

    MaxQBi P12791.
    PaxDbi P12791.
    PRIDEi P12791.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Organism-specific databases

    MGIi MGI:88598. Cyp2b10.

    Phylogenomic databases

    eggNOGi COG2124.
    HOGENOMi HOG000036992.
    HOVERGENi HBG015789.
    InParanoidi P12791.
    PhylomeDBi P12791.

    Miscellaneous databases

    ChiTaRSi Cyp2b10. mouse.
    PROi P12791.
    SOURCEi Search...

    Gene expression databases

    Genevestigatori P12791.

    Family and domain databases

    Gene3Di 1.10.630.10. 1 hit.
    InterProi IPR001128. Cyt_P450.
    IPR002401. Cyt_P450_E_grp-I.
    IPR008068. Cyt_P450_E_grp-I_CYP2B-like.
    [Graphical view ]
    Pfami PF00067. p450. 1 hit.
    [Graphical view ]
    PRINTSi PR00463. EP450I.
    PR01685. EP450ICYP2B.
    PR00385. P450.
    SUPFAMi SSF48264. SSF48264. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Rip locus: regulation of female-specific isozyme (I-P-450(16 alpha) of testosterone 16 alpha-hydroxylase in mouse liver, chromosome localization, and cloning of P-450 cDNA."
      Noshiro M., Lakso M., Kawajiri K., Negishi M.
      Biochemistry 27:6434-6443(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    2. "Isolation of a new mouse cDNA clone: hybrid form of cytochrome P450 2b10 and NADPH-cytochrome P450 oxidoreductase."
      Damon M., Fautrel A., Marc N., Guillouzo A., Corcos L.
      Biochem. Biophys. Res. Commun. 226:900-905(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-452 (ISOFORM 2).
      Strain: BALB/c.
      Tissue: Liver.
    3. "Purification and characterization of a mouse liver cytochrome P-450 induced by cannabidiol."
      Bornheim L.M., Correia M.A.
      Mol. Pharmacol. 36:377-383(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 1-15, INDUCTION.

    Entry informationi

    Entry nameiCP2BA_MOUSE
    AccessioniPrimary (citable) accession number: P12791
    Secondary accession number(s): Q62397
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1989
    Last sequence update: October 1, 1989
    Last modified: October 1, 2014
    This is version 117 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3