Reviewed, UniProtKB/Swiss-Prot P12790 (CP2B9_MOUSE)

Names and origin

Protein names

Recommended name:
    Cytochrome P450 2B9
    EC=1.14.14.1
Alternative name(s):
    CYPIIB9
    Testosterone 16-alpha hydroxylase
    P450-16-alpha
    Clone PF26

Gene names

Name:	Cyp2b9
Synonyms:	Cyp2b-9, Rip

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	491 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
Catalytic activity	RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.
Cofactor	Heme group By similarity.
Subcellular location	Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell microsome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aromatase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 491	491	Cytochrome P450 2B9		PRO_0000051684
Sites
	Metal binding	436	1	Iron (heme axial ligand) By similarity
Amino acid modifications
	Modified residue	128	1	Phosphoserine; by PKA By similarity

Sequences

	Sequence		Length	Mass (Da)
	P12790-1 [UniParc]. Last modified October 1, 1989. Version 1. Checksum: E626F61F136CF697	FASTA	491	55,760
	10 20 30 40 50 60 MDPSVLLLLA VLLSLFLLLV RGHAKIHGHL PPGPHPLPLL GNLLQMDRGG LLKCFIQLQE 70 80 90 100 110 120 KHGDVFTVHL GPRPVVVLCG TQTIREALVD HAEAFSGRGT IAAAQLVMQD YGIFFASGQR 130 140 150 160 170 180 WKTLRRFSLA TMKEFGMGKR SVEERIKEEA QCLVEELKKY QGVPLDPTFL FQCITANIIC 190 200 210 220 230 240 SIVFGERFDY TDDQFLHLLN LMYKIFSLLS SFSGQMFELF SGFLKYFPGV HRQIVKKQQE 250 260 270 280 290 300 LLDYIAHSVE KHKATLDPSA PRDYIDTYLL RMEKEKSNHN TEFHHQNLMM SVLSLFFVGT 310 320 330 340 350 360 ETTSATLHYG VLLMLKYPHV TEKVQKEIDQ VIGSHRLPTL DDRTKMPYTD AVIHEIQRFS 370 380 390 400 410 420 DLVPIGLPHK VIKDTLFRGY LLPKNTEVYP VLSSALHDPQ YFEQPDKFNP EQFLDANGAL 430 440 450 460 470 480 KKCEAFLPFS TGKRICLGES IARNELFIFF TTILQNFSVA SPVAPKDIDL TPKESGIGKI 490 PPAHQIYFLA R

References

[1]	"Rip locus: regulation of female-specific isozyme (I-P-450(16 alpha) of testosterone 16 alpha-hydroxylase in mouse liver, chromosome localization, and cloning of P-450 cDNA." Noshiro M., Lakso M., Kawajiri K., Negishi M. Biochemistry 27:6434-6443(1988) [PubMed: 3219345] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M21855 mRNA. Translation: AAA40424.1.
IPI	IPI00134518.
PIR	A31047.
UniGene	Mm.14413
3D structure databases
HSSP	HSSP built from PDB template 1DT6 based on UniProtKB P00179.
SMR	P12790. Positions 28-491.
ModBase	Search...
Genome annotation databases
Ensembl	ENSMUSG00000040660. Mus musculus. [Contig view]
Organism-specific databases
MGI	MGI:88600. Cyp2b9.
Phylogenomic databases
HOGENOM	P12790.
HOVERGEN	P12790.
Enzyme and pathway databases
BRENDA	1.14.14.1. 244.
Gene expression databases
ArrayExpress	P12790.
Bgee	P12790.
GermOnline	ENSMUSG00000040660. Mus musculus.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017973. Cyt_P450_C. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. IPR008068. Cyt_P450_E_grp-I_CYP2B-like. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR01685. EP450ICYP2B. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

CP2B9_MOUSE

Accession

Primary (citable) accession number: P12790

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	October 1, 1989
Last modified:	June 16, 2009
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)