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Protein

Cytochrome P450 2B9

Gene

Cyp2b9

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.

Catalytic activityi

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi436 – 4361Iron (heme axial ligand)By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_274299. Xenobiotics.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 2B9 (EC:1.14.14.1)
Alternative name(s):
CYPIIB9
Cytochrome P450 clone PF26
Cytochrome P450-16-alpha
Testosterone 16-alpha hydroxylase
Gene namesi
Name:Cyp2b9
Synonyms:Cyp2b-9, Rip
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:88600. Cyp2b9.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

Pathology & Biotechi

Chemistry

DrugBankiDB08834. Tauroursodeoxycholic acid.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 491491Cytochrome P450 2B9PRO_0000051684Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei128 – 1281Phosphoserine; by PKABy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP12790.
PRIDEiP12790.

PTM databases

PhosphoSiteiP12790.

Expressioni

Gene expression databases

ExpressionAtlasiP12790. baseline and differential.
GenevestigatoriP12790.

Interactioni

Protein-protein interaction databases

IntActiP12790. 1 interaction.
STRINGi10090.ENSMUSP00000080846.

Structurei

3D structure databases

ProteinModelPortaliP12790.
SMRiP12790. Positions 28-491.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

eggNOGiCOG2124.
GeneTreeiENSGT00760000118775.
HOGENOMiHOG000036992.
HOVERGENiHBG015789.
InParanoidiP12790.
KOiK07412.
OrthoDBiEOG77M8NM.
TreeFamiTF352043.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008068. Cyt_P450_E_grp-I_CYP2B-like.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR01685. EP450ICYP2B.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P12790-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDPSVLLLLA VLLSLFLLLV RGHAKIHGHL PPGPHPLPLL GNLLQMDRGG
60 70 80 90 100
LLKCFIQLQE KHGDVFTVHL GPRPVVVLCG TQTIREALVD HAEAFSGRGT
110 120 130 140 150
IAAAQLVMQD YGIFFASGQR WKTLRRFSLA TMKEFGMGKR SVEERIKEEA
160 170 180 190 200
QCLVEELKKY QGVPLDPTFL FQCITANIIC SIVFGERFDY TDDQFLHLLN
210 220 230 240 250
LMYKIFSLLS SFSGQMFELF SGFLKYFPGV HRQIVKKQQE LLDYIAHSVE
260 270 280 290 300
KHKATLDPSA PRDYIDTYLL RMEKEKSNHN TEFHHQNLMM SVLSLFFAGT
310 320 330 340 350
ETTSATLHYG VLLMLKYPHV TEKVQKEIDQ VIGSHRLPTL DDRTKMPYTD
360 370 380 390 400
AVIHEIQRFS DLVPIGLPHK VIKDTLFRGY LLPKNTEVYP VLSSALHDPQ
410 420 430 440 450
YFEQPDKFNP EHFLDANGAL KKCEAFLPFS TGKRICLGES IARNELFIFF
460 470 480 490
TTILQNFSVA SPVAPKDIDL TPKESGIGKI PPAHQIYFLA R
Length:491
Mass (Da):55,741
Last modified:September 18, 2013 - v2
Checksum:iC1E790A7DD1A7298
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti298 – 2981A → V in AAA40424 (PubMed:3219345).Curated
Sequence conflicti412 – 4121H → Q in AAA40424 (PubMed:3219345).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M21855 mRNA. Translation: AAA40424.1.
M60273
, M60267, M60268, M60269, M60270, M60271, M60272 Genomic DNA. Translation: AAA03648.1.
AC157782 Genomic DNA. No translation available.
BC120525 mRNA. Translation: AAI20526.1.
BC120527 mRNA. Translation: AAI20528.1.
CCDSiCCDS21001.1.
PIRiA31047.
I84735.
RefSeqiNP_034130.1. NM_010000.2.
UniGeneiMm.14413.

Genome annotation databases

EnsembliENSMUST00000082214; ENSMUSP00000080846; ENSMUSG00000040660.
GeneIDi13094.
KEGGimmu:13094.
UCSCiuc009fuh.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M21855 mRNA. Translation: AAA40424.1.
M60273
, M60267, M60268, M60269, M60270, M60271, M60272 Genomic DNA. Translation: AAA03648.1.
AC157782 Genomic DNA. No translation available.
BC120525 mRNA. Translation: AAI20526.1.
BC120527 mRNA. Translation: AAI20528.1.
CCDSiCCDS21001.1.
PIRiA31047.
I84735.
RefSeqiNP_034130.1. NM_010000.2.
UniGeneiMm.14413.

3D structure databases

ProteinModelPortaliP12790.
SMRiP12790. Positions 28-491.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP12790. 1 interaction.
STRINGi10090.ENSMUSP00000080846.

Chemistry

DrugBankiDB08834. Tauroursodeoxycholic acid.

PTM databases

PhosphoSiteiP12790.

Proteomic databases

PaxDbiP12790.
PRIDEiP12790.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000082214; ENSMUSP00000080846; ENSMUSG00000040660.
GeneIDi13094.
KEGGimmu:13094.
UCSCiuc009fuh.1. mouse.

Organism-specific databases

CTDi13094.
MGIiMGI:88600. Cyp2b9.

Phylogenomic databases

eggNOGiCOG2124.
GeneTreeiENSGT00760000118775.
HOGENOMiHOG000036992.
HOVERGENiHBG015789.
InParanoidiP12790.
KOiK07412.
OrthoDBiEOG77M8NM.
TreeFamiTF352043.

Enzyme and pathway databases

ReactomeiREACT_274299. Xenobiotics.

Miscellaneous databases

NextBioi283070.
PROiP12790.
SOURCEiSearch...

Gene expression databases

ExpressionAtlasiP12790. baseline and differential.
GenevestigatoriP12790.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008068. Cyt_P450_E_grp-I_CYP2B-like.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR01685. EP450ICYP2B.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Rip locus: regulation of female-specific isozyme (I-P-450(16 alpha) of testosterone 16 alpha-hydroxylase in mouse liver, chromosome localization, and cloning of P-450 cDNA."
    Noshiro M., Lakso M., Kawajiri K., Negishi M.
    Biochemistry 27:6434-6443(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver.
  2. "Structures and characterization of sex-specific mouse cytochrome P-450 genes as members within a large family. Duplication boundary and evolution."
    Lakso M., Masaki R., Noshiro M., Negishi M.
    Eur. J. Biochem. 195:477-486(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.

Entry informationi

Entry nameiCP2B9_MOUSE
AccessioniPrimary (citable) accession number: P12790
Secondary accession number(s): Q64463
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: September 18, 2013
Last modified: April 1, 2015
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.