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Protein

Cytochrome P450 2B5

Gene

CYP2B5

Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.

Catalytic activityi

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi436 – 4361Iron (heme axial ligand)

GO - Molecular functioni

  1. aromatase activity Source: UniProtKB-EC
  2. heme binding Source: InterPro
  3. iron ion binding Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 2B5 (EC:1.14.14.1)
Alternative name(s):
CYPIIB5
Cytochrome P450 form HP1
Cytochrome P450 type B2
Gene namesi
Name:CYP2B5
OrganismiOryctolagus cuniculus (Rabbit)
Taxonomic identifieri9986 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
ProteomesiUP000001811: Unplaced

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 491491Cytochrome P450 2B5PRO_0000051682Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei128 – 1281Phosphoserine; by PKABy similarity

Keywords - PTMi

Phosphoprotein

Expressioni

Inductioni

By phenobarbital.

Structurei

3D structure databases

ProteinModelPortaliP12789.
SMRiP12789. Positions 28-491.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

eggNOGiCOG2124.
HOVERGENiHBG015789.
InParanoidiP12789.
KOiK07412.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008068. Cyt_P450_E_grp-I_CYP2B-like.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR01685. EP450ICYP2B.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P12789-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEFSLLLLLA FLAGLLLLLF RGHPKAHGRL PPGPPPLPVL GNLLQMDRKG
60 70 80 90 100
LLRSFLRLRE KYGDVFTVYL GSRPVVVLCG TDAIREALVD QAEAFSGRGK
110 120 130 140 150
IAVVDPIFQG YGVFFANGEH WRALRRFSLA TMRDFGMGKR SVEERIQEEA
160 170 180 190 200
RCLVEELRKS KGALLDNTLL FHSVTSNIIC SIVFGKRFDY KDPVFLRLLD
210 220 230 240 250
LFFQSFSLIS SFSSQVFELF PGFLKHFPGT HRQIYRNLQE INTFIGQTVE
260 270 280 290 300
KHRATLDPSN PRDFIDVYLL RMEKDKSDPS SEFHHRNLIL TVLTLFFAGT
310 320 330 340 350
ETTSTTLRYG FLLMLKYPHV TERVQKEIEQ VIGSHRPPAL DDRAKMPYTD
360 370 380 390 400
AVIHEIQRLG DLVPFGAPHM VTKDTQFRGY VIPKNTEVFP VLSSALHDPR
410 420 430 440 450
YFETPNTFNP GHFLDADGAL KRNEGFMPFS LGKRICLGEG IARTELFLFF
460 470 480 490
TTILQNFSIA SPVPPEDIDL TPRESGVGNV PPSYQIRFLA R
Length:491
Mass (Da):55,770
Last modified:October 1, 1989 - v1
Checksum:iABE2B978B8408DCF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M20855 mRNA. Translation: AAA31223.1.
S78830 mRNA. Translation: AAB35177.1.
PIRiD27717.
S31278.
RefSeqiNP_001164601.1. NM_001171130.1.
UniGeneiOcu.6935.

Genome annotation databases

GeneIDi100328947.
KEGGiocu:100328947.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M20855 mRNA. Translation: AAA31223.1.
S78830 mRNA. Translation: AAB35177.1.
PIRiD27717.
S31278.
RefSeqiNP_001164601.1. NM_001171130.1.
UniGeneiOcu.6935.

3D structure databases

ProteinModelPortaliP12789.
SMRiP12789. Positions 28-491.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100328947.
KEGGiocu:100328947.

Organism-specific databases

CTDi100328947.

Phylogenomic databases

eggNOGiCOG2124.
HOVERGENiHBG015789.
InParanoidiP12789.
KOiK07412.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008068. Cyt_P450_E_grp-I_CYP2B-like.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR01685. EP450ICYP2B.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Primary structures of multiple forms of cytochrome P-450 isozyme 2 derived from rabbit pulmonary and hepatic cDNAs."
    Gasser R., Negishi M., Philpot R.M.
    Mol. Pharmacol. 33:22-30(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Rabbit liver cytochrome P-450 2B5: high-level expression of the full-length protein in Escherichia coli, purification, and catalytic activity."
    Lehnerer M., Schulze J., Petzold A., Bernhardt R., Hlavica P.
    Biochim. Biophys. Acta 1245:107-115(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-56.

Entry informationi

Entry nameiCP2B5_RABIT
AccessioniPrimary (citable) accession number: P12789
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 1, 1989
Last modified: February 4, 2015
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.