Reviewed, UniProtKB/Swiss-Prot P12789 (CP2B5_RABIT)

Names and origin

Protein names

Recommended name:
    Cytochrome P450 2B5
    EC=1.14.14.1
Alternative name(s):
    CYPIIB5
    P450 type B2
    P450 form HP1

Gene names

Name:

CYP2B5

Organism

Oryctolagus cuniculus (Rabbit)

Taxonomic identifier

9986 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Lagomorpha › Leporidae › Oryctolagus

Protein attributes

Sequence length	491 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
Catalytic activity	RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.
Cofactor	Heme group By similarity.
Subcellular location	Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
Induction	By phenobarbital.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell microsome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aromatase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 491	491	Cytochrome P450 2B5		PRO_0000051682
Sites
	Metal binding	436	1	Iron (heme axial ligand)
Amino acid modifications
	Modified residue	128	1	Phosphoserine; by PKA By similarity

Sequences

	Sequence		Length	Mass (Da)
	P12789-1 [UniParc]. Last modified October 1, 1989. Version 1. Checksum: ABE2B978B8408DCF	FASTA	491	55,770
	10 20 30 40 50 60 MEFSLLLLLA FLAGLLLLLF RGHPKAHGRL PPGPPPLPVL GNLLQMDRKG LLRSFLRLRE 70 80 90 100 110 120 KYGDVFTVYL GSRPVVVLCG TDAIREALVD QAEAFSGRGK IAVVDPIFQG YGVFFANGEH 130 140 150 160 170 180 WRALRRFSLA TMRDFGMGKR SVEERIQEEA RCLVEELRKS KGALLDNTLL FHSVTSNIIC 190 200 210 220 230 240 SIVFGKRFDY KDPVFLRLLD LFFQSFSLIS SFSSQVFELF PGFLKHFPGT HRQIYRNLQE 250 260 270 280 290 300 INTFIGQTVE KHRATLDPSN PRDFIDVYLL RMEKDKSDPS SEFHHRNLIL TVLTLFFAGT 310 320 330 340 350 360 ETTSTTLRYG FLLMLKYPHV TERVQKEIEQ VIGSHRPPAL DDRAKMPYTD AVIHEIQRLG 370 380 390 400 410 420 DLVPFGAPHM VTKDTQFRGY VIPKNTEVFP VLSSALHDPR YFETPNTFNP GHFLDADGAL 430 440 450 460 470 480 KRNEGFMPFS LGKRICLGEG IARTELFLFF TTILQNFSIA SPVPPEDIDL TPRESGVGNV 490 PPSYQIRFLA R

References

[1]	"Primary structures of multiple forms of cytochrome P-450 isozyme 2 derived from rabbit pulmonary and hepatic cDNAs." Gasser R., Negishi M., Philpot R.M. Mol. Pharmacol. 33:22-30(1988) [PubMed: 2826996] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Rabbit liver cytochrome P-450 2B5: high-level expression of the full-length protein in Escherichia coli, purification, and catalytic activity." Lehnerer M., Schulze J., Petzold A., Bernhardt R., Hlavica P. Biochim. Biophys. Acta 1245:107-115(1995) [PubMed: 7654758] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-56.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M20855 mRNA. Translation: AAA31223.1. S78830 mRNA. Translation: AAB35177.1.
PIR	D27717. S31278.
3D structure databases
HSSP	HSSP built from PDB template 1DT6 based on UniProtKB P00179.
SMR	P12789. Positions 28-491.
ModBase	Search...
Phylogenomic databases
HOVERGEN	P12789.
Enzyme and pathway databases
BRENDA	1.14.14.1. 255.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017973. Cyt_P450_C. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. IPR008068. Cyt_P450_E_grp-I_CYP2B-like. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR01685. EP450ICYP2B. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CP2B5_RABIT

Accession

Primary (citable) accession number: P12789

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	October 1, 1989
Last modified:	June 16, 2009
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)