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Reviewed, UniProtKB/Swiss-Prot P12788 (TRY4_RAT)

Last modified June 16, 2009. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Trypsin-4
    EC=3.4.21.4
Alternative name(s):
    Trypsin IV
    Pretrypsinogen IV
Gene names
Name: Try4
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length247 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level.

General annotation (Comments)

Catalytic activity

Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

Cofactor

Binds 1 calcium ion per subunit By similarity.

Subcellular location

Secretedextracellular space.

Induction

By CCK.

Sequence similarities

Belongs to the peptidase S1 family.

Contains 1 peptidase S1 domain.

Ontologies

Keywords
   Biological processDigestion
   Cellular componentSecreted
   DomainSignal
   LigandCalcium
Metal-binding
   Molecular functionHydrolase
Protease
Serine protease
   PTMDisulfide bond
Zymogen
Gene Ontology (GO)
   Biological processdigestion

Inferred from electronic annotation. Source: UniProtKB-KW

proteolysis

Inferred from electronic annotation. Source: InterPro

   Cellular componentextracellular space

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functioncalcium ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

serine-type endopeptidase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1515
Propeptide16 – 238Activation peptide
PRO_0000028213
Chain24 – 247224Trypsin-4
PRO_0000028214

Regions

Domain24 – 245222Peptidase S1

Sites

Active site641Charge relay system By similarity
Active site1081Charge relay system By similarity
Active site2011Charge relay system By similarity
Metal binding761Calcium By similarity
Metal binding781Calcium; via carbonyl oxygen By similarity
Metal binding811Calcium; via carbonyl oxygen By similarity
Metal binding861Calcium By similarity
Site1951Required for specificity By similarity

Amino acid modifications

Disulfide bond30 ↔ 161 By similarity
Disulfide bond49 ↔ 65 By similarity
Disulfide bond133 ↔ 234 By similarity
Disulfide bond140 ↔ 207 By similarity
Disulfide bond172 ↔ 186 By similarity
Disulfide bond197 ↔ 221 By similarity

Sequences

Sequence LengthMass (Da)Tools
P12788-1 [UniParc].

Last modified October 1, 1989. Version 1.
Checksum: AE987B9D32D58F93

FASTA24726,573
        10         20         30         40         50         60 
MKISIFFAFL GAAVALPVND DDKIVGGYTC PKHLVPYQVS LHDGISHQCG GSLISDQWVL 

        70         80         90        100        110        120 
SAAHCYKRKL QVRLGEHNIH VLEGGEQFID AEKIIRHPEY NKDTLDNDIM LIKLKSPAVL 

       130        140        150        160        170        180 
NSQVSTVSLP RSCASTDAQC LVSGWGNTVS IGGKYPALLQ CLEAPVLSAS SCKKSYPGQI 

       190        200        210        220        230        240 
TSNMFCLGFL EGGKDSCDGD SGGPVVCNGE IQGIVSWGSV CAMRGKPGVY TKVCNYLSWI 


QETMANN 

« Hide

References

[1]"A fourth trypsinogen (P23) in the rat pancreas induced by CCK."
Luetcke H.A., Rausch U., Vasiloudes P., Scheele G.A., Kern H.F.
Nucleic Acids Res. 17:6736-6736(1989) [PubMed: 2780302] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Wistar.
Tissue: Pancreas.

Cross-references

Sequence databases

X15679 mRNA. Translation: CAA33718.1.
IPIIPI00200668.
PIRS05494.
RefSeqNP_775423.1.
UniGeneRn.10387

3D structure databases

HSSPHSSP built from PDB template 1EZX based on UniProtKB P00760.
SMRP12788. Positions 24-247.
ModBaseSearch...

Protein family/group databases

MEROPSS01.183.

Genome annotation databases

EnsemblENSRNOG00000013245. Rattus norvegicus. [Contig view]
GeneID286960.
KEGGrno:286960.

Organism-specific databases

RGD708585. LOC286960.

Phylogenomic databases

HOVERGENP12788.
OMAP12788. GWGNTVS.

Enzyme and pathway databases

BRENDA3.4.21.4. 248.

Gene expression databases

ArrayExpressP12788.
GermOnlineENSRNOG00000013245. Rattus norvegicus.

Family and domain databases

InterProIPR018114. Peptidase_S1/S6_AS.
IPR001254. Peptidase_S1_S6.
IPR001314. Peptidase_S1A.
[Graphical view]
PfamPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSPR00722. CHYMOTRYPSIN.
SMARTSM00020. Tryp_SPc. 1 hit.
[Graphical view]
PROSITEPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio625158.

Entry information

Entry nameTRY4_RAT
AccessionPrimary (citable) accession number: P12788
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 1, 1989
Last modified: June 16, 2009
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

Peptidase families

Classification of peptidase families and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents