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Protein

Trypsin-4

Gene

Try4

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

Cofactori

Ca2+By similarityNote: Binds 1 Ca2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei64Charge relay systemBy similarity1
Metal bindingi76CalciumBy similarity1
Metal bindingi78Calcium; via carbonyl oxygenBy similarity1
Metal bindingi81Calcium; via carbonyl oxygenBy similarity1
Metal bindingi86CalciumBy similarity1
Active sitei108Charge relay systemBy similarity1
Sitei195Required for specificityBy similarity1
Active sitei201Charge relay systemBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Serine protease
Biological processDigestion
LigandCalcium, Metal-binding

Protein family/group databases

MEROPSiS01.183.

Names & Taxonomyi

Protein namesi
Recommended name:
Trypsin-4 (EC:3.4.21.4)
Alternative name(s):
Pretrypsinogen IV
Trypsin IV
Gene namesi
Name:Try4
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 4

Organism-specific databases

RGDi708585. LOC286960.

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 15Add BLAST15
PropeptideiPRO_000002821316 – 23Activation peptide8
ChainiPRO_000002821424 – 247Trypsin-4Add BLAST224

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi30 ↔ 161PROSITE-ProRule annotation
Disulfide bondi49 ↔ 65PROSITE-ProRule annotation
Disulfide bondi133 ↔ 234PROSITE-ProRule annotation
Disulfide bondi140 ↔ 207PROSITE-ProRule annotation
Disulfide bondi172 ↔ 186PROSITE-ProRule annotation
Disulfide bondi197 ↔ 221PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Zymogen

Proteomic databases

PaxDbiP12788.
PRIDEiP12788.

Expressioni

Inductioni

By CCK.

Gene expression databases

GenevisibleiP12788. RN.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000017852.

Structurei

3D structure databases

ProteinModelPortaliP12788.
SMRiP12788.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini24 – 245Peptidase S1PROSITE-ProRule annotationAdd BLAST222

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
GeneTreeiENSGT00760000118862.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiP12788.
KOiK01312.
OMAiCYKSRVE.
OrthoDBiEOG091G0DF7.
PhylomeDBiP12788.
TreeFamiTF331065.

Family and domain databases

CDDicd00190. Tryp_SPc. 1 hit.
InterProiView protein in InterPro
IPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
PfamiView protein in Pfam
PF00089. Trypsin. 1 hit.
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiView protein in SMART
SM00020. Tryp_SPc. 1 hit.
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiView protein in PROSITE
PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P12788-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKISIFFAFL GAAVALPVND DDKIVGGYTC PKHLVPYQVS LHDGISHQCG
60 70 80 90 100
GSLISDQWVL SAAHCYKRKL QVRLGEHNIH VLEGGEQFID AEKIIRHPEY
110 120 130 140 150
NKDTLDNDIM LIKLKSPAVL NSQVSTVSLP RSCASTDAQC LVSGWGNTVS
160 170 180 190 200
IGGKYPALLQ CLEAPVLSAS SCKKSYPGQI TSNMFCLGFL EGGKDSCDGD
210 220 230 240
SGGPVVCNGE IQGIVSWGSV CAMRGKPGVY TKVCNYLSWI QETMANN
Length:247
Mass (Da):26,573
Last modified:October 1, 1989 - v1
Checksum:iAE987B9D32D58F93
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15679 mRNA. Translation: CAA33718.1.
PIRiS05494.
RefSeqiNP_775423.1. NM_173301.1.
UniGeneiRn.10387.

Genome annotation databases

EnsembliENSRNOT00000017852; ENSRNOP00000017852; ENSRNOG00000013245.
GeneIDi286960.
KEGGirno:286960.
UCSCiRGD:708585. rat.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiTRY4_RAT
AccessioniPrimary (citable) accession number: P12788
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 1, 1989
Last modified: June 7, 2017
This is version 133 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families