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Protein

Trypsin-4

Gene

Try4

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

Cofactori

Ca2+By similarityNote: Binds 1 Ca2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei64 – 641Charge relay systemBy similarity
Metal bindingi76 – 761CalciumBy similarity
Metal bindingi78 – 781Calcium; via carbonyl oxygenBy similarity
Metal bindingi81 – 811Calcium; via carbonyl oxygenBy similarity
Metal bindingi86 – 861CalciumBy similarity
Active sitei108 – 1081Charge relay systemBy similarity
Sitei195 – 1951Required for specificityBy similarity
Active sitei201 – 2011Charge relay systemBy similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. serine-type endopeptidase activity Source: InterPro

GO - Biological processi

  1. digestion Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Biological processi

Digestion

Keywords - Ligandi

Calcium, Metal-binding

Protein family/group databases

MEROPSiS01.183.

Names & Taxonomyi

Protein namesi
Recommended name:
Trypsin-4 (EC:3.4.21.4)
Alternative name(s):
Pretrypsinogen IV
Trypsin IV
Gene namesi
Name:Try4
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 4

Organism-specific databases

RGDi708585. LOC286960.

Subcellular locationi

GO - Cellular componenti

  1. extracellular space Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1515Add
BLAST
Propeptidei16 – 238Activation peptidePRO_0000028213
Chaini24 – 247224Trypsin-4PRO_0000028214Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi30 ↔ 161PROSITE-ProRule annotation
Disulfide bondi49 ↔ 65PROSITE-ProRule annotation
Disulfide bondi133 ↔ 234PROSITE-ProRule annotation
Disulfide bondi140 ↔ 207PROSITE-ProRule annotation
Disulfide bondi172 ↔ 186PROSITE-ProRule annotation
Disulfide bondi197 ↔ 221PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Zymogen

Proteomic databases

PaxDbiP12788.
PRIDEiP12788.

Expressioni

Inductioni

By CCK.

Gene expression databases

GenevestigatoriP12788.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000017852.

Structurei

3D structure databases

ProteinModelPortaliP12788.
SMRiP12788. Positions 24-247.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini24 – 245222Peptidase S1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG5640.
GeneTreeiENSGT00760000118862.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiP12788.
KOiK01312.
OMAiCASTDAQ.
OrthoDBiEOG75B84T.
PhylomeDBiP12788.
TreeFamiTF331065.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P12788-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKISIFFAFL GAAVALPVND DDKIVGGYTC PKHLVPYQVS LHDGISHQCG
60 70 80 90 100
GSLISDQWVL SAAHCYKRKL QVRLGEHNIH VLEGGEQFID AEKIIRHPEY
110 120 130 140 150
NKDTLDNDIM LIKLKSPAVL NSQVSTVSLP RSCASTDAQC LVSGWGNTVS
160 170 180 190 200
IGGKYPALLQ CLEAPVLSAS SCKKSYPGQI TSNMFCLGFL EGGKDSCDGD
210 220 230 240
SGGPVVCNGE IQGIVSWGSV CAMRGKPGVY TKVCNYLSWI QETMANN
Length:247
Mass (Da):26,573
Last modified:October 1, 1989 - v1
Checksum:iAE987B9D32D58F93
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15679 mRNA. Translation: CAA33718.1.
PIRiS05494.
RefSeqiNP_775423.1. NM_173301.1.
UniGeneiRn.10387.

Genome annotation databases

EnsembliENSRNOT00000017852; ENSRNOP00000017852; ENSRNOG00000013245.
GeneIDi286960.
KEGGirno:286960.
UCSCiRGD:708585. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15679 mRNA. Translation: CAA33718.1.
PIRiS05494.
RefSeqiNP_775423.1. NM_173301.1.
UniGeneiRn.10387.

3D structure databases

ProteinModelPortaliP12788.
SMRiP12788. Positions 24-247.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000017852.

Protein family/group databases

MEROPSiS01.183.

Proteomic databases

PaxDbiP12788.
PRIDEiP12788.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000017852; ENSRNOP00000017852; ENSRNOG00000013245.
GeneIDi286960.
KEGGirno:286960.
UCSCiRGD:708585. rat.

Organism-specific databases

RGDi708585. LOC286960.

Phylogenomic databases

eggNOGiCOG5640.
GeneTreeiENSGT00760000118862.
HOGENOMiHOG000251820.
HOVERGENiHBG013304.
InParanoidiP12788.
KOiK01312.
OMAiCASTDAQ.
OrthoDBiEOG75B84T.
PhylomeDBiP12788.
TreeFamiTF331065.

Miscellaneous databases

NextBioi625158.

Gene expression databases

GenevestigatoriP12788.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "A fourth trypsinogen (P23) in the rat pancreas induced by CCK."
    Luetcke H.A., Rausch U., Vasiloudes P., Scheele G.A., Kern H.F.
    Nucleic Acids Res. 17:6736-6736(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Wistar.
    Tissue: Pancreas.

Entry informationi

Entry nameiTRY4_RAT
AccessioniPrimary (citable) accession number: P12788
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 1, 1989
Last modified: January 7, 2015
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.