Reviewed, UniProtKB/Swiss-Prot P12788 (TRY4_RAT)

Names and origin

Protein names

Recommended name:
    Trypsin-4
    EC=3.4.21.4
Alternative name(s):
    Trypsin IV
    Pretrypsinogen IV

Gene names

Name:

Try4

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	247 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.
Cofactor	Binds 1 calcium ion per subunit By similarity.
Subcellular location	Secreted › extracellular space.
Induction	By CCK.
Sequence similarities	Belongs to the peptidase S1 family. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Biological process	Digestion
Cellular component	Secreted
Domain	Signal
Ligand	Calcium Metal-binding
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Zymogen
Gene Ontology (GO)
Biological process	digestion Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 15	15
	Propeptide	16 – 23	8	Activation peptide		PRO_0000028213
	Chain	24 – 247	224	Trypsin-4		PRO_0000028214
Regions
	Domain	24 – 245	222	Peptidase S1
Sites
	Active site	64	1	Charge relay system By similarity
	Active site	108	1	Charge relay system By similarity
	Active site	201	1	Charge relay system By similarity
	Metal binding	76	1	Calcium By similarity
	Metal binding	78	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	81	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	86	1	Calcium By similarity
	Site	195	1	Required for specificity By similarity
Amino acid modifications
	Disulfide bond	30 ↔ 161		By similarity
	Disulfide bond	49 ↔ 65		By similarity
	Disulfide bond	133 ↔ 234		By similarity
	Disulfide bond	140 ↔ 207		By similarity
	Disulfide bond	172 ↔ 186		By similarity
	Disulfide bond	197 ↔ 221		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P12788-1 [UniParc]. Last modified October 1, 1989. Version 1. Checksum: AE987B9D32D58F93	FASTA	247	26,573
	10 20 30 40 50 60 MKISIFFAFL GAAVALPVND DDKIVGGYTC PKHLVPYQVS LHDGISHQCG GSLISDQWVL 70 80 90 100 110 120 SAAHCYKRKL QVRLGEHNIH VLEGGEQFID AEKIIRHPEY NKDTLDNDIM LIKLKSPAVL 130 140 150 160 170 180 NSQVSTVSLP RSCASTDAQC LVSGWGNTVS IGGKYPALLQ CLEAPVLSAS SCKKSYPGQI 190 200 210 220 230 240 TSNMFCLGFL EGGKDSCDGD SGGPVVCNGE IQGIVSWGSV CAMRGKPGVY TKVCNYLSWI QETMANN

References

[1]	"A fourth trypsinogen (P23) in the rat pancreas induced by CCK." Luetcke H.A., Rausch U., Vasiloudes P., Scheele G.A., Kern H.F. Nucleic Acids Res. 17:6736-6736(1989) [PubMed: 2780302] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Wistar. Tissue: Pancreas.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X15679 mRNA. Translation: CAA33718.1.
IPI	IPI00200668.
PIR	S05494.
RefSeq	NP_775423.1.
UniGene	Rn.10387
3D structure databases
HSSP	HSSP built from PDB template 1EZX based on UniProtKB P00760.
SMR	P12788. Positions 24-247.
ModBase	Search...
Protein family/group databases
MEROPS	S01.183.
Genome annotation databases
Ensembl	ENSRNOG00000013245. Rattus norvegicus. [Contig view]
GeneID	286960.
KEGG	rno:286960.
Organism-specific databases
RGD	708585. LOC286960.
Phylogenomic databases
HOVERGEN	P12788.
OMA	P12788. GWGNTVS.
Enzyme and pathway databases
BRENDA	3.4.21.4. 248.
Gene expression databases
ArrayExpress	P12788.
GermOnline	ENSRNOG00000013245. Rattus norvegicus.
Family and domain databases
InterPro	IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	625158.

Entry information

Entry name

TRY4_RAT

Accession

Primary (citable) accession number: P12788

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	October 1, 1989
Last modified:	June 16, 2009
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)