P12788 (TRY4_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Trypsin-4
EC=3.4.21.4

Alternative name(s):
Pretrypsinogen IV
Trypsin IV

Gene names

Name:

Try4

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	247 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.
Cofactor	Binds 1 calcium ion per subunit By similarity.
Subcellular location	Secreted › extracellular space.
Induction	By CCK.
Sequence similarities	Belongs to the peptidase S1 family. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Biological process	Digestion
Cellular component	Secreted
Domain	Signal
Ligand	Calcium Metal-binding
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Zymogen
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	digestion Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 15	15
	Propeptide	16 – 23	8	Activation peptide		PRO_0000028213
	Chain	24 – 247	224	Trypsin-4		PRO_0000028214
Regions
	Domain	24 – 245	222	Peptidase S1
Sites
	Active site	64	1	Charge relay system By similarity
	Active site	108	1	Charge relay system By similarity
	Active site	201	1	Charge relay system By similarity
	Metal binding	76	1	Calcium By similarity
	Metal binding	78	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	81	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	86	1	Calcium By similarity
	Site	195	1	Required for specificity By similarity
Amino acid modifications
	Disulfide bond	30 ↔ 161		By similarity
	Disulfide bond	49 ↔ 65		By similarity
	Disulfide bond	133 ↔ 234		By similarity
	Disulfide bond	140 ↔ 207		By similarity
	Disulfide bond	172 ↔ 186		By similarity
	Disulfide bond	197 ↔ 221		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P12788 [UniParc]. Last modified October 1, 1989. Version 1. Checksum: AE987B9D32D58F93	FASTA	247	26,573
	10 20 30 40 50 60 MKISIFFAFL GAAVALPVND DDKIVGGYTC PKHLVPYQVS LHDGISHQCG GSLISDQWVL 70 80 90 100 110 120 SAAHCYKRKL QVRLGEHNIH VLEGGEQFID AEKIIRHPEY NKDTLDNDIM LIKLKSPAVL 130 140 150 160 170 180 NSQVSTVSLP RSCASTDAQC LVSGWGNTVS IGGKYPALLQ CLEAPVLSAS SCKKSYPGQI 190 200 210 220 230 240 TSNMFCLGFL EGGKDSCDGD SGGPVVCNGE IQGIVSWGSV CAMRGKPGVY TKVCNYLSWI QETMANN

References

[1]	"A fourth trypsinogen (P23) in the rat pancreas induced by CCK." Luetcke H.A., Rausch U., Vasiloudes P., Scheele G.A., Kern H.F. Nucleic Acids Res. 17:6736-6736(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Wistar. Tissue: Pancreas.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X15679 mRNA. Translation: CAA33718.1.
IPI	IPI00200668.
PIR	S05494.
RefSeq	NP_775423.1. NM_173301.1.
UniGene	Rn.10387.
3D structure databases
ProteinModelPortal	P12788.
SMR	P12788. Positions 24-247.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000017852.
Protein family/group databases
MEROPS	S01.183.
Proteomic databases
PaxDb	P12788.
PRIDE	P12788.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000017852; ENSRNOP00000017852; ENSRNOG00000013245.
GeneID	286960.
KEGG	rno:286960.
UCSC	RGD:708585. rat.
Organism-specific databases
RGD	708585. LOC286960.
Phylogenomic databases
eggNOG	COG5640.
GeneTree	ENSGT00680000099557.
HOGENOM	HOG000251820.
HOVERGEN	HBG013304.
InParanoid	P12788.
KO	K01312.
OMA	WIQETIT.
OrthoDB	EOG4SN1QN.
Gene expression databases
Genevestigator	P12788.
GermOnline	ENSRNOG00000013245. Rattus norvegicus.
Family and domain databases
InterPro	IPR001254. Peptidase_S1. IPR018114. Peptidase_S1_AS. IPR001314. Peptidase_S1A. IPR009003. Trypsin-like_Pept_dom. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
SUPFAM	SSF50494. Pept_Ser_Cys. 1 hit.
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	625158.

Entry information

Entry name

TRY4_RAT

Accession

Primary (citable) accession number: P12788

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	October 1, 1989
Last modified:	April 3, 2013
This is version 105 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program