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P12782 (PGKH_WHEAT) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoglycerate kinase, chloroplastic

EC=2.7.2.3
OrganismTriticum aestivum (Wheat) [Complete proteome]
Taxonomic identifier4565 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladePooideaeTriticeaeTriticum

Protein attributes

Sequence length480 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate. HAMAP-Rule MF_00145

Pathway

Carbohydrate biosynthesis; Calvin cycle. HAMAP-Rule MF_00145

Subunit structure

Monomer.

Subcellular location

Plastidchloroplast HAMAP-Rule MF_00145.

Sequence similarities

Belongs to the phosphoglycerate kinase family.

Ontologies

Keywords
   Biological processCalvin cycle
   Cellular componentChloroplast
Plastid
   DomainTransit peptide
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processglycolytic process

Inferred from electronic annotation. Source: InterPro

reductive pentose-phosphate cycle

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentchloroplast

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

phosphoglycerate kinase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – ?7272Chloroplast Potential
Chain?73 – 480408Phosphoglycerate kinase, chloroplastic HAMAP-Rule MF_00145
PRO_0000023894

Regions

Nucleotide binding428 – 4314ATP By similarity
Region96 – 983Substrate binding By similarity
Region135 – 1384Substrate binding By similarity

Sites

Binding site1121Substrate By similarity
Binding site1931Substrate By similarity
Binding site2261Substrate By similarity
Binding site2771ATP By similarity
Binding site3681ATP; via carbonyl oxygen By similarity
Binding site3991ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
P12782 [UniParc].

Last modified October 1, 1989. Version 1.
Checksum: 3EBA1F378DAB16CC

FASTA48049,840
        10         20         30         40         50         60 
MASTAAPPAA LVARRAASAS VAAPLRGAGL AAGCQPARSL AFAAGADPRL AVHVASRCRA 

        70         80         90        100        110        120 
ASAARGTRAV ATMAKKSVGD LTAADLEGKR VLVRADLNVP LDDNQNITDD TRIRAAIPTI 

       130        140        150        160        170        180 
KYLLSNGAKV ILTSHLGRPK GVTPKFSLAP LVPRLSELLG IEVKKAEDVI GPEVEKLVAD 

       190        200        210        220        230        240 
LANGAVLLLE NVRFYKEEEK NDPEFAKKLA SLADLFVNDA FGTAHRAHAS TEGVTKFLKP 

       250        260        270        280        290        300 
SVAGFLLQKE LDYLDGAVSN PKRPFAAIVG GSKVSSKIGV IESLLEKCDI LLLGGGMIFT 

       310        320        330        340        350        360 
FYKAQGLSVG SSLVEEDKLE LATSLLAKAK AKGVSLLLPS DVIIADKFAP DANSQTVPAS 

       370        380        390        400        410        420 
AIPDGWMGLD IGPDSVKTFN DALDTTQTII WNGPMGVFEF DKFAVGTESI AKKLAELSKK 

       430        440        450        460        470        480 
GVTTIIGGGD SVAAVEKVGV ADVMSHISTG GGASLELLEG KELPGVVALD EGVMTRSVTV 

« Hide

References

[1]"Wheat phosphoglycerate kinase: evidence for recombination between the genes for the chloroplastic and cytosolic enzymes."
Longstaff M., Raines C.A., McMorrow E.M., Bradbeer J.W., Dyer T.A.
Nucleic Acids Res. 17:6569-6580(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Mardler.
Tissue: Leaf.
[2]"Nucleotide sequence of a wheat chloroplastic Phosphoglycerate kinase gene."
Jones P.G., Raines C., Lloyd J.C.
Plant Physiol. 107:1483-1484(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: cv. Chinese Spring.
Tissue: Etiolated shoot.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X15233 mRNA. Translation: CAA33303.1.
X73528 Genomic DNA. Translation: CAA51931.1.
PIRTVWTGC. S05967.
UniGeneTa.90680.

3D structure databases

ProteinModelPortalP12782.
SMRP12782. Positions 73-471.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEP12782.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

GrameneP12782.

Enzyme and pathway databases

UniPathwayUPA00116.

Family and domain databases

Gene3D3.40.50.1260. 1 hit.
3.40.50.1270. 1 hit.
HAMAPMF_00145. Phosphoglyc_kinase.
InterProIPR001576. Phosphoglycerate_kinase.
IPR015901. Phosphoglycerate_kinase_C.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
[Graphical view]
PANTHERPTHR11406. PTHR11406. 1 hit.
PfamPF00162. PGK. 1 hit.
[Graphical view]
PIRSFPIRSF000724. Pgk. 1 hit.
PRINTSPR00477. PHGLYCKINASE.
SUPFAMSSF53748. SSF53748. 1 hit.
PROSITEPS00111. PGLYCERATE_KINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePGKH_WHEAT
AccessionPrimary (citable) accession number: P12782
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 1, 1989
Last modified: June 11, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways