Reviewed, UniProtKB/Swiss-Prot P12782 (PGKH_WHEAT)

Names and origin

Protein names	Recommended name:     Phosphoglycerate kinase, chloroplastic     EC=2.7.2.3
Organism	Triticum aestivum (Wheat)
Taxonomic identifier	4565 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Pooideae › Triticeae › Triticum

Protein attributes

Sequence length	480 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate.
Pathway	Carbohydrate biosynthesis; Calvin cycle.
Subunit structure	Monomer.
Subcellular location	Plastid › chloroplast.
Sequence similarities	Belongs to the phosphoglycerate kinase family.

Ontologies

Keywords
Biological process	Calvin cycle
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: InterPro reductive pentose-phosphate cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoglycerate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?72	72	Chloroplast Potential
	Chain	?73 – 480	408	Phosphoglycerate kinase, chloroplastic		PRO_0000023894
Regions
	Nucleotide binding	428 – 431	4	ATP By similarity
	Region	96 – 98	3	Substrate binding By similarity
	Region	135 – 138	4	Substrate binding By similarity
Sites
	Binding site	112	1	Substrate By similarity
	Binding site	193	1	Substrate By similarity
	Binding site	226	1	Substrate By similarity
	Binding site	277	1	ATP By similarity
	Binding site	368	1	ATP; via carbonyl oxygen By similarity
	Binding site	399	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P12782-1 [UniParc]. Last modified October 1, 1989. Version 1. Checksum: 3EBA1F378DAB16CC	FASTA	480	49,840
	10 20 30 40 50 60 MASTAAPPAA LVARRAASAS VAAPLRGAGL AAGCQPARSL AFAAGADPRL AVHVASRCRA 70 80 90 100 110 120 ASAARGTRAV ATMAKKSVGD LTAADLEGKR VLVRADLNVP LDDNQNITDD TRIRAAIPTI 130 140 150 160 170 180 KYLLSNGAKV ILTSHLGRPK GVTPKFSLAP LVPRLSELLG IEVKKAEDVI GPEVEKLVAD 190 200 210 220 230 240 LANGAVLLLE NVRFYKEEEK NDPEFAKKLA SLADLFVNDA FGTAHRAHAS TEGVTKFLKP 250 260 270 280 290 300 SVAGFLLQKE LDYLDGAVSN PKRPFAAIVG GSKVSSKIGV IESLLEKCDI LLLGGGMIFT 310 320 330 340 350 360 FYKAQGLSVG SSLVEEDKLE LATSLLAKAK AKGVSLLLPS DVIIADKFAP DANSQTVPAS 370 380 390 400 410 420 AIPDGWMGLD IGPDSVKTFN DALDTTQTII WNGPMGVFEF DKFAVGTESI AKKLAELSKK 430 440 450 460 470 480 GVTTIIGGGD SVAAVEKVGV ADVMSHISTG GGASLELLEG KELPGVVALD EGVMTRSVTV

References

[1]	"Wheat phosphoglycerate kinase: evidence for recombination between the genes for the chloroplastic and cytosolic enzymes." Longstaff M., Raines C.A., McMorrow E.M., Bradbeer J.W., Dyer T.A. Nucleic Acids Res. 17:6569-6580(1989) [PubMed: 2780287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Mardler. Tissue: Leaf.
[2]	"Nucleotide sequence of a wheat chloroplastic Phosphoglycerate kinase gene." Jones P.G., Raines C., Lloyd J.C. Plant Physiol. 107:1483-1484(1995) [PubMed: 7770546] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Chinese Spring. Tissue: Etiolated shoot.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X15233 mRNA. Translation: CAA33303.1. X73528 Genomic DNA. Translation: CAA51931.1.
PIR	TVWTGC. S05967.
UniGene	Ta.2307
3D structure databases
SMR	P12782. Positions 73-471.
ModBase	Search...
Organism-specific databases
Gramene	P12782.
Enzyme and pathway databases
BRENDA	2.7.2.3. 253.
Family and domain databases
InterPro	IPR001576. Phosphoglycerate_kinase. IPR015901. Phosphoglycerate_kinase_C. IPR015911. Phosphoglycerate_kinase_CS. IPR015824. Phosphoglycerate_kinase_N. [Graphical view]
Gene3D	G3DSA:3.40.50.1270. Phosphoglycerate_kinase_C. 1 hit. G3DSA:3.40.50.1260. Phosphoglycerate_kinase_N. 1 hit.
PANTHER	PTHR11406. PGK. 1 hit.
Pfam	PF00162. PGK. 1 hit. [Graphical view]
PRINTS	PR00477. PHGLYCKINASE.
PROSITE	PS00111. PGLYCERATE_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PGKH_WHEAT

Accession

Primary (citable) accession number: P12782

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	October 1, 1989
Last modified:	January 19, 2010
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)