Reviewed, UniProtKB/Swiss-Prot P12764 (ALLS_DIPPU)

Names and origin

Protein names	Recommended name:     Allatostatins Cleaved into the following 13 chains:     1- Recommended name:             Allatostatin-1                 Short name=AST1     2- Recommended name:             Allatostatin-2                 Short name=AST2         Alternative name(s):             ASB2             ASA5     3- Recommended name:             Allatostatin-3                 Short name=AST3     4- Recommended name:             Allatostatin-4                 Short name=AST4         Alternative name(s):             ASA7     5- Recommended name:             Allatostatin-5                 Short name=AST5         Alternative name(s):             ASA4     6- Recommended name:             Allatostatin-6                 Short name=AST6     7- Recommended name:             Allatostatin-7                 Short name=AST7         Alternative name(s):             ASA1     8- Recommended name:             Allatostatin-8                 Short name=AST8         Alternative name(s):             ASA3     9- Recommended name:             Allatostatin-9                 Short name=AST9         Alternative name(s):             ASA2     10- Recommended name:             Allatostatin-10                 Short name=AST10     11- Recommended name:             Allatostatin-11                 Short name=AST11         Alternative name(s):             ASA6     12- Recommended name:             Allatostatin-12                 Short name=AST12     13- Recommended name:             Allatostatin-13                 Short name=AST13
Organism	Diploptera punctata (Pacific beetle cockroach)
Taxonomic identifier	6984 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Orthopteroidea › Dictyoptera › Blattaria › Blaberidae › Diplopterinae › Diploptera

Protein attributes

Sequence length	370 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Neuropeptide inhibitors of juvenile hormone synthesis.
Subcellular location	Secreted.
Tissue specificity	Brain, suboesophageal ganglion and corpus allatum.
Sequence similarities	Belongs to the allatostatin family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Neuropeptide
PTM	Amidation Cleavage on pair of basic residues
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	neuropeptide signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	neuropeptide hormone activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 27

27

Potential

Propeptide

28 – 65

38

PRO_0000001129

Peptide

68 – 73

6

Allatostatin-1

PRO_0000001130

Peptide

77 – 94

18

Allatostatin-2 Ref.3

PRO_0000001131

Peptide

98 – 105

8

Allatostatin-3

PRO_0000001132

Peptide

109 – 117

9

Allatostatin-4

PRO_0000001133

Propeptide

121 – 151

31

PRO_0000001134

Peptide

154 – 161

8

Allatostatin-5 Ref.2

PRO_0000001135

Peptide

165 – 172

8

Allatostatin-6

PRO_0000001136

Peptide

176 – 188

13

Allatostatin-7 Ref.5

PRO_0000001137

Peptide

192 – 200

9

Allatostatin-8

PRO_0000001138

Peptide

204 – 213

10

Allatostatin-9

PRO_0000001139

Peptide

217 – 232

16

Allatostatin-10

PRO_0000001140

Propeptide

236 – 251

16

PRO_0000001141

Peptide

254 – 264

11

Allatostatin-11

PRO_0000001142

Propeptide

268 – 345

78

PRO_0000001143

Peptide

348 – 353

6

Allatostatin-12

PRO_0000001144

Peptide

357 – 364

8

Allatostatin-13

PRO_0000001145

Propeptide

368 – 370

3

PRO_0000001146

Amino acid modifications

Modified residue

73

1

Leucine amide

Modified residue

94

1

Leucine amide

Modified residue

105

1

Leucine amide

Modified residue

117

1

Leucine amide

Modified residue

161

1

Leucine amide

Modified residue

172

1

Leucine amide

Modified residue

188

1

Leucine amide

Modified residue

200

1

Leucine amide

Modified residue

213

1

Leucine amide

Modified residue

232

1

Leucine amide

Modified residue

264

1

Leucine amide

Modified residue

353

1

Leucine amide

Modified residue

364

1

Isoleucine amide

Secondary structure

1

.

.

.

.

.

370

Helix Strand Turn

Details...

Helix

157 – 159

3

Beta strand

194 – 198

5

Sequences

	Sequence		Length	Mass (Da)
	P12764-1 [UniParc]. Last modified October 1, 1996. Version 2. Checksum: A5AA8E7C13DE8ED9	FASTA	370	41,562
	10 20 30 40 50 60 MSGPRTCFCL PSALVLVLLS LSTSALGTAP EPSGVHEESP AGGGTDLLPH PEDLSASDNP 70 80 90 100 110 120 DLEFVKRLYD FGLGKRAYSY VSEYKRLPVY NFGLGKRSKM YGFGLGKRDG RMYSFGLGKR 130 140 150 160 170 180 DYDYYGEEDE DDQQAIGDED IEESDVGDLM DKRDRLYSFG LGKRARPYSF GLGKRAPSGA 190 200 210 220 230 240 QRLYGFGLGK RGGSLYSFGL GKRGDGRLYA FGLGKRPVNS GRSSGSRFNF GLGKRSDDID 250 260 270 280 290 300 FRELEEKFAE DKRYPQEHRF SFGLGKREVE PSELEAVRNE EKDNSSVHDK KNNTNDMHSG 310 320 330 340 350 360 ERIKRSLHYP FGIRKLESSY DLNSASSLNS EENDDITPEE FSRMVRRPFN FGLGKRIPMY 370 DFGIGKRSER

References

[1]	"Molecular cloning of the gene for the allatostatin family of neuropeptides from the cockroach Diploptera punctata." Donly B.C., Ding Q., Tobe S.S., Bendena W.G. Proc. Natl. Acad. Sci. U.S.A. 90:8807-8811(1993) [PubMed: 8415611] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[2]	"Primary structure of four allatostatins: neuropeptide inhibitors of juvenile hormone synthesis." Woodhead A.P., Stay B., Seidel S.L., Khan M.A., Tobe S.S. Proc. Natl. Acad. Sci. U.S.A. 86:5997-6001(1989) [PubMed: 2762309] [Abstract] Cited for: PROTEIN SEQUENCE OF 154-161; 176-188; 192-200 AND 204-213. Tissue: Brain.
[3]	"Identity of a second type of allatostatin from cockroach brains: an octadecapeptide amide with a tyrosine-rich address sequence." Pratt G.E., Farnsworth D.E., Fok K.F., Siegel N.R., McCormack A.L., Shabanowitz J., Hunt D.F., Feyereisen R. Proc. Natl. Acad. Sci. U.S.A. 88:2412-2416(1991) [PubMed: 2006179] [Abstract] Cited for: PROTEIN SEQUENCE OF 77-94. Tissue: Brain.
[4]	"Expression of the allatostatin gene in endocrine cells of the cockroach midgut." Reichwald K., Unnithan G.C., Davis N.T., Agricola H., Feyereisen R. Proc. Natl. Acad. Sci. U.S.A. 91:11894-11898(1994) [PubMed: 7991553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 77-189.
[5]	"Identification of an allatostatin from adult Diploptera punctata." Pratt G.E., Farnsworth D.E., Siegel N.R., Fok K.F., Feyereisen R. Biochem. Biophys. Res. Commun. 163:1243-1247(1989) [PubMed: 2783135] [Abstract] Cited for: PROTEIN SEQUENCE OF 176-188 (AST7). Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U00444 mRNA. Translation: AAA18260.1.
S74578 mRNA. Translation: AAB32720.2.

PIR

A48252.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2JQS	NMR	-	A	154-161	[»]
2JQU	NMR	-	A	192-200	[»]

ModBase

Search...

Family and domain databases

InterPro

IPR010276. Allatostatin.
[Graphical view]

Pfam

PF05953. Allatostatin. 13 hits.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

ALLS_DIPPU

Accession

Primary (citable) accession number: P12764
Secondary accession number(s): P12765 , P12766, P12767, P22410

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	October 1, 1996
Last modified:	January 19, 2010
This is version 55 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)