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Protein

Ski-like protein

Gene

SKIL

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May have regulatory role in cell division or differentiation in response to extracellular signals.

GO - Molecular functioni

  • chromatin binding Source: UniProtKB
  • protein complex binding Source: UniProtKB
  • protein domain specific binding Source: UniProtKB
  • repressing transcription factor binding Source: GO_Central
  • RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: NTNU_SB
  • SMAD binding Source: UniProtKB
  • transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding Source: NTNU_SB
  • transcription corepressor activity Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Enzyme and pathway databases

BioCyciZFISH:ENSG00000136603-MONOMER.
ReactomeiR-HSA-2173795. Downregulation of SMAD2/3:SMAD4 transcriptional activity.
SignaLinkiP12757.
SIGNORiP12757.

Names & Taxonomyi

Protein namesi
Recommended name:
Ski-like protein
Alternative name(s):
Ski-related oncogene
Ski-related protein
Gene namesi
Name:SKIL
Synonyms:SNO
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

HGNCiHGNC:10897. SKIL.

Subcellular locationi

GO - Cellular componenti

  • acrosomal vesicle Source: Ensembl
  • cytoplasm Source: UniProtKB
  • nucleoplasm Source: Reactome
  • nucleus Source: UniProtKB
  • PML body Source: UniProtKB
  • protein complex Source: MGI
  • transcriptional repressor complex Source: GO_Central
  • transcription factor complex Source: GO_Central
Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

DisGeNETi6498.
OpenTargetsiENSG00000136603.
PharmGKBiPA35797.

Polymorphism and mutation databases

BioMutaiSKIL.
DMDMi313104010.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001293871 – 684Ski-like proteinAdd BLAST684

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki50Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei452PhosphoserineCombined sources1
Cross-linki527Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP12757.
MaxQBiP12757.
PaxDbiP12757.
PeptideAtlasiP12757.
PRIDEiP12757.

PTM databases

iPTMnetiP12757.
PhosphoSitePlusiP12757.

Expressioni

Tissue specificityi

Isoform SNON and isoform SNOA are widely expressed. Highest expression is found in skeletal muscle, followed by placenta and lung. Lowest expression in heart, brain and pancreas. Isoform SNOI expression is restricted to skeletal muscle.1 Publication

Gene expression databases

BgeeiENSG00000136603.
CleanExiHS_SKIL.
ExpressionAtlasiP12757. baseline and differential.
GenevisibleiP12757. HS.

Organism-specific databases

HPAiHPA008472.

Interactioni

Subunit structurei

Interacts with CPNE4 (via VWFA domain) (By similarity). Interacts with SMAD2, SMAD3 and RNF111 (PubMed:17591695). Isoform 1 interacts with WWP1 (PubMed:15221015).By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
AESQ081173EBI-2902468,EBI-717810
DRG1Q9Y2953EBI-2902468,EBI-719554
Ncor1Q609742EBI-2902468,EBI-349004From a different organism.
NXF3Q9H4D53EBI-2902468,EBI-750038
OIP5O434823EBI-2902468,EBI-536879
SMAD4Q134853EBI-2902468,EBI-347263
STK16O757163EBI-2902468,EBI-749295
VPS28Q548N13EBI-2902468,EBI-10243107

GO - Molecular functioni

  • protein complex binding Source: UniProtKB
  • protein domain specific binding Source: UniProtKB
  • repressing transcription factor binding Source: GO_Central
  • SMAD binding Source: UniProtKB

Protein-protein interaction databases

BioGridi112389. 96 interactors.
DIPiDIP-42138N.
IntActiP12757. 78 interactors.
MINTiMINT-1179766.
STRINGi9606.ENSP00000259119.

Structurei

Secondary structure

1684
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi137 – 139Combined sources3
Beta strandi145 – 149Combined sources5
Beta strandi152 – 159Combined sources8
Beta strandi162 – 166Combined sources5
Helixi167 – 172Combined sources6
Turni173 – 177Combined sources5
Helixi180 – 190Combined sources11
Helixi199 – 207Combined sources9
Beta strandi219 – 222Combined sources4
Helixi223 – 234Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3EQ5X-ray2.45A/B/C/D/E/F/G/H/I/J/K/L137-238[»]
5C4VX-ray2.60B/D/F238-356[»]
ProteinModelPortaliP12757.
SMRiP12757.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP12757.

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili536 – 684Sequence analysisAdd BLAST149

Sequence similaritiesi

Belongs to the SKI family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IFDK. Eukaryota.
ENOG410Y8AU. LUCA.
GeneTreeiENSGT00530000063040.
HOGENOMiHOG000039989.
HOVERGENiHBG006599.
InParanoidiP12757.
KOiK18499.
OMAiMEEFYIE.
OrthoDBiEOG091G0O02.
PhylomeDBiP12757.
TreeFamiTF324133.

Family and domain databases

Gene3Di3.10.260.20. 1 hit.
3.10.390.10. 1 hit.
InterProiIPR014890. c-SKI_SMAD4-bd_dom.
IPR009061. DNA-bd_dom_put.
IPR010919. SAND_dom-like.
IPR028373. Ski-rel_Sno.
IPR003380. Transform_Ski.
IPR023216. Tscrpt_reg_SKI_SnoN.
[Graphical view]
PANTHERiPTHR10005. PTHR10005. 1 hit.
PTHR10005:SF3. PTHR10005:SF3. 1 hit.
PfamiPF08782. c-SKI_SMAD_bind. 1 hit.
PF02437. Ski_Sno. 1 hit.
[Graphical view]
SMARTiSM01046. c-SKI_SMAD_bind. 1 hit.
[Graphical view]
SUPFAMiSSF46955. SSF46955. 1 hit.
SSF63763. SSF63763. 1 hit.

Sequences (5)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform SNON (identifier: P12757-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MENLQTNFSL VQGSTKKLNG MGDDGSPPAK KMITDIHANG KTINKVPTVK
60 70 80 90 100
KEHLDDYGEA PVETDGEHVK RTCTSVPETL HLNPSLKHTL AQFHLSSQSS
110 120 130 140 150
LGGPAAFSAR HSQESMSPTV FLPLPSPQVL PGPLLIPSDS STELTQTVLE
160 170 180 190 200
GESISCFQVG GEKRLCLPQV LNSVLREFTL QQINTVCDEL YIYCSRCTSD
210 220 230 240 250
QLHILKVLGI LPFNAPSCGL ITLTDAQRLC NALLRPRTFP QNGSVLPAKS
260 270 280 290 300
SLAQLKETGS AFEVEHECLG KCQGLFAPQF YVQPDAPCIQ CLECCGMFAP
310 320 330 340 350
QTFVMHSHRS PDKRTCHWGF ESAKWHCYLH VNQKYLGTPE EKKLKIILEE
360 370 380 390 400
MKEKFSMRSG KRNQSKTDAP SGMELQSWYP VIKQEGDHVS QTHSFLHPSY
410 420 430 440 450
YLYMCDKVVA PNVSLTSAVS QSKELTKTEA SKSISRQSEK AHSSGKLQKT
460 470 480 490 500
VSYPDVSLEE QEKMDLKTSR ELCSRLDASI SNNSTSKRKS ESATCNLVRD
510 520 530 540 550
INKVGIGLVA AASSPLLVKD VICEDDKGKI MEEVMRTYLK QQEKLNLILQ
560 570 580 590 600
KKQQLQMEVK MLSSSKSMKE LTEEQQNLQK ELESLQNEHA QRMEEFYVEQ
610 620 630 640 650
KDLEKKLEQI MKQKCTCDSN LEKDKEAEYA GQLAELRQRL DHAEADRQEL
660 670 680
QDELRQEREA RQKLEMMIKE LKLQILKSSK TAKE
Length:684
Mass (Da):76,976
Last modified:November 30, 2010 - v2
Checksum:i6A04D4ECEC214CF1
GO
Isoform SNOA (identifier: P12757-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     367-415: TDAPSGMELQ...DKVVAPNVSL → ASFLYQFLIM...YRLIYLYCSF
     416-684: Missing.

Show »
Length:415
Mass (Da):46,335
Checksum:iA2F3E39AE47BB69E
GO
Isoform SNON2 (identifier: P12757-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     431-431: S → N
     432-477: Missing.

Show »
Length:638
Mass (Da):71,767
Checksum:i3963A1976EF1673C
GO
Isoform SNOI (identifier: P12757-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     400-684: Missing.

Show »
Length:399
Mass (Da):44,203
Checksum:i0AFD7E54B7AB5139
GO
Isoform 5 (identifier: P12757-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-20: Missing.

Note: No experimental confirmation available.
Show »
Length:664
Mass (Da):74,784
Checksum:iA9B843A38DAF1ED5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti13G → R in CAA79636 (PubMed:8233802).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_01167738A → V.3 PublicationsCorresponds to variant rs3772173dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0400991 – 20Missing in isoform 5. 1 PublicationAdd BLAST20
Alternative sequenceiVSP_004392367 – 415TDAPS…PNVSL → ASFLYQFLIMVMVYFEMKIL CLVCNLTCMLNIAHATTTKY RLIYLYCSF in isoform SNOA. 1 PublicationAdd BLAST49
Alternative sequenceiVSP_004393400 – 684Missing in isoform SNOI. 1 PublicationAdd BLAST285
Alternative sequenceiVSP_004394416 – 684Missing in isoform SNOA. 1 PublicationAdd BLAST269
Alternative sequenceiVSP_004395431S → N in isoform SNON2. 1 Publication1
Alternative sequenceiVSP_004396432 – 477Missing in isoform SNON2. 1 PublicationAdd BLAST46

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15219 mRNA. Translation: CAA33289.1.
X15217 mRNA. Translation: CAA33287.1.
U70730 mRNA. Translation: AAB65850.1.
AK300053 mRNA. Translation: BAG61862.1.
AC073288 Genomic DNA. No translation available.
BC059386 mRNA. Translation: AAH59386.1.
Z19588 mRNA. Translation: CAA79636.1.
CCDSiCCDS33890.1. [P12757-1]
CCDS46953.1. [P12757-3]
CCDS46954.1. [P12757-5]
PIRiS06052. TVHUSN.
S06054. TVHUSA.
RefSeqiNP_001138569.1. NM_001145097.2. [P12757-3]
NP_001138570.1. NM_001145098.2. [P12757-5]
NP_001234937.1. NM_001248008.1. [P12757-1]
NP_005405.2. NM_005414.4. [P12757-1]
XP_005247778.1. XM_005247721.1. [P12757-1]
XP_006713798.1. XM_006713735.1. [P12757-1]
UniGeneiHs.536655.
Hs.581632.

Genome annotation databases

EnsembliENST00000259119; ENSP00000259119; ENSG00000136603. [P12757-1]
ENST00000413427; ENSP00000400193; ENSG00000136603. [P12757-3]
ENST00000426052; ENSP00000406520; ENSG00000136603. [P12757-5]
ENST00000458537; ENSP00000415243; ENSG00000136603. [P12757-1]
GeneIDi6498.
KEGGihsa:6498.
UCSCiuc003fgu.4. human. [P12757-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15219 mRNA. Translation: CAA33289.1.
X15217 mRNA. Translation: CAA33287.1.
U70730 mRNA. Translation: AAB65850.1.
AK300053 mRNA. Translation: BAG61862.1.
AC073288 Genomic DNA. No translation available.
BC059386 mRNA. Translation: AAH59386.1.
Z19588 mRNA. Translation: CAA79636.1.
CCDSiCCDS33890.1. [P12757-1]
CCDS46953.1. [P12757-3]
CCDS46954.1. [P12757-5]
PIRiS06052. TVHUSN.
S06054. TVHUSA.
RefSeqiNP_001138569.1. NM_001145097.2. [P12757-3]
NP_001138570.1. NM_001145098.2. [P12757-5]
NP_001234937.1. NM_001248008.1. [P12757-1]
NP_005405.2. NM_005414.4. [P12757-1]
XP_005247778.1. XM_005247721.1. [P12757-1]
XP_006713798.1. XM_006713735.1. [P12757-1]
UniGeneiHs.536655.
Hs.581632.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3EQ5X-ray2.45A/B/C/D/E/F/G/H/I/J/K/L137-238[»]
5C4VX-ray2.60B/D/F238-356[»]
ProteinModelPortaliP12757.
SMRiP12757.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi112389. 96 interactors.
DIPiDIP-42138N.
IntActiP12757. 78 interactors.
MINTiMINT-1179766.
STRINGi9606.ENSP00000259119.

PTM databases

iPTMnetiP12757.
PhosphoSitePlusiP12757.

Polymorphism and mutation databases

BioMutaiSKIL.
DMDMi313104010.

Proteomic databases

EPDiP12757.
MaxQBiP12757.
PaxDbiP12757.
PeptideAtlasiP12757.
PRIDEiP12757.

Protocols and materials databases

DNASUi6498.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000259119; ENSP00000259119; ENSG00000136603. [P12757-1]
ENST00000413427; ENSP00000400193; ENSG00000136603. [P12757-3]
ENST00000426052; ENSP00000406520; ENSG00000136603. [P12757-5]
ENST00000458537; ENSP00000415243; ENSG00000136603. [P12757-1]
GeneIDi6498.
KEGGihsa:6498.
UCSCiuc003fgu.4. human. [P12757-1]

Organism-specific databases

CTDi6498.
DisGeNETi6498.
GeneCardsiSKIL.
HGNCiHGNC:10897. SKIL.
HPAiHPA008472.
MIMi165340. gene.
neXtProtiNX_P12757.
OpenTargetsiENSG00000136603.
PharmGKBiPA35797.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IFDK. Eukaryota.
ENOG410Y8AU. LUCA.
GeneTreeiENSGT00530000063040.
HOGENOMiHOG000039989.
HOVERGENiHBG006599.
InParanoidiP12757.
KOiK18499.
OMAiMEEFYIE.
OrthoDBiEOG091G0O02.
PhylomeDBiP12757.
TreeFamiTF324133.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000136603-MONOMER.
ReactomeiR-HSA-2173795. Downregulation of SMAD2/3:SMAD4 transcriptional activity.
SignaLinkiP12757.
SIGNORiP12757.

Miscellaneous databases

ChiTaRSiSKIL. human.
EvolutionaryTraceiP12757.
GeneWikiiSKIL.
GenomeRNAii6498.
PROiP12757.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000136603.
CleanExiHS_SKIL.
ExpressionAtlasiP12757. baseline and differential.
GenevisibleiP12757. HS.

Family and domain databases

Gene3Di3.10.260.20. 1 hit.
3.10.390.10. 1 hit.
InterProiIPR014890. c-SKI_SMAD4-bd_dom.
IPR009061. DNA-bd_dom_put.
IPR010919. SAND_dom-like.
IPR028373. Ski-rel_Sno.
IPR003380. Transform_Ski.
IPR023216. Tscrpt_reg_SKI_SnoN.
[Graphical view]
PANTHERiPTHR10005. PTHR10005. 1 hit.
PTHR10005:SF3. PTHR10005:SF3. 1 hit.
PfamiPF08782. c-SKI_SMAD_bind. 1 hit.
PF02437. Ski_Sno. 1 hit.
[Graphical view]
SMARTiSM01046. c-SKI_SMAD_bind. 1 hit.
[Graphical view]
SUPFAMiSSF46955. SSF46955. 1 hit.
SSF63763. SSF63763. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSKIL_HUMAN
AccessioniPrimary (citable) accession number: P12757
Secondary accession number(s): A6NGT1
, B4DT50, O00464, P12756, Q07501
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: November 30, 2010
Last modified: November 30, 2016
This is version 158 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.