Reviewed, UniProtKB/Swiss-Prot P12755 (SKI_HUMAN)

Names and origin

Protein names

Recommended name:
    Ski oncogene
Alternative name(s):
    C-ski

Gene names

Name:

SKI

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	728 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May play a role in terminal differentiation of skeletal muscle cells but not in the determination of cells to the myogenic lineage.
Subunit structure	Interacts with SMAD2, SMAD3 and SMAD4. Interacts with HIPK2. Part of a complex with HIPK2 and SMAD1/2/3. Ref.3
Subcellular location	Nucleus.
Sequence similarities	Belongs to the SKI family.

Ontologies

Keywords
Cellular component	Nucleus
Disease	Proto-oncogene
Domain	Coiled coil Repeat
Technical term	3D-structure
Gene Ontology (GO)
Biological process	BMP signaling pathway Inferred from Experiment. Source: Reactome cell differentiation Non-traceable author statement. Source: UniProtKB
Cellular component	nucleoplasm Inferred from Experiment. Source: Reactome
Molecular function	nucleotide binding Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
NCOR1	O75376	1	EBI-347281,EBI-347233
SIN3A	Q96ST3	1	EBI-347281,EBI-347218
SMAD3	P84022	1	EBI-347281,EBI-347161
SMAD4	Q13485	2	EBI-347281,EBI-347263

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 728

728

Ski oncogene

PRO_0000129382

Regions

Coiled coil

536 – 710

175

Potential

Secondary structure

1

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728

Helix Strand Turn

Details...

Beta strand

101 – 105

5

Beta strand

108 – 115

8

Beta strand

118 – 122

5

Helix

123 – 127

5

Turn

128 – 133

6

Helix

136 – 145

10

Helix

155 – 163

9

Beta strand

175 – 178

4

Helix

179 – 190

12

Beta strand

219 – 222

4

Beta strand

228 – 232

5

Helix

234 – 236

3

Beta strand

245 – 247

3

Turn

248 – 250

3

Helix

256 – 259

4

Beta strand

269 – 275

7

Helix

278 – 280

3

Helix

281 – 284

4

Helix

296 – 310

15

Sequences

	Sequence		Length	Mass (Da)
	P12755-1 [UniParc]. Last modified October 1, 1989. Version 1. Checksum: 9B78C4840A28C2DA	FASTA	728	80,005
	10 20 30 40 50 60 MEAAAGGRGC FQPHPGLQKT LEQFHLSSMS SLGGPAAFSA RWAQEAYKKE SAKEAGAAAV 70 80 90 100 110 120 PAPVPAATEP PPVLHLPAIQ PPPPVLPGPF FMPSDRSTER CETVLEGETI SCFVVGGEKR 130 140 150 160 170 180 LCLPQILNSV LRDFSLQQIN AVCDELHIYC SRCTADQLEI LKVMGILPFS APSCGLITKT 190 200 210 220 230 240 DAERLCNALL YGGAYPPPCK KELAASLALG LELSERSVRV YHECFGKCKG LLVPELYSSP 250 260 270 280 290 300 SAACIQCLDC RLMYPPHKFV VHSHKALENR TCHWGFDSAN WRAYILLSQD YTGKEEQARL 310 320 330 340 350 360 GRCLDDVKEK FDYGNKYKRR VPRVSSEPPA SIRPKTDDTS SQSPAPSEKD KPSSWLRTLA 370 380 390 400 410 420 GSSNKSLGCV HPRQRLSAFR PWSPAVSASE KELSPHLPAL IRDSFYSYKS FETAVAPNVA 430 440 450 460 470 480 LAPPAQQKVV SSPPCAAAVS RAPEPLATCT QPRKRKLTVD TPGAPETLAP VAAPEEDKDS 490 500 510 520 530 540 EAEVEVESRE EFTSSLSSLS SPSFTSSSSA KDLGSPGARA LPSAVPDAAA PADAPSGLEA 550 560 570 580 590 600 ELEHLRQALE GGLDTKEAKE KFLHEVVKMR VKQEEKLSAA LQAKRSLHQE LEFLRVAKKE 610 620 630 640 650 660 KLREATEAKR NLRKEIERLR AENEKKMKEA NESRLRLKRE LEQARQARVC DKGCEAGRLR 670 680 690 700 710 720 AKYSAQIEDL QVKLQHAEAD REQLRADLLR EREAREHLEK VVKELQEQLW PRARPEAAGS EGAAELEP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation of human cDNA clones of ski and the ski-related gene, sno." Nomura N., Sasamoto S., Ishii S., Date T., Matsui M., Ishizaki R. Nucleic Acids Res. 17:5489-5500(1989) [PubMed: 2762147] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed: 16710414] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"Requirement of the co-repressor homeodomain-interacting protein kinase 2 for ski-mediated inhibition of bone morphogenetic protein-induced transcriptional activation." Harada J., Kokura K., Kanei-Ishii C., Nomura T., Khan M.M., Kim Y., Ishii S. J. Biol. Chem. 278:38998-39005(2003) [PubMed: 12874272] [Abstract] Cited for: INTERACTION WITH HIPK2; SMAD2; SMAD3 AND SMAD4.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

X15218 mRNA. Translation: CAA33288.1.
AL590822 Genomic DNA. Translation: CAI12482.1.

IPI

IPI00220921.

PIR

TVHUSK. S06053.

RefSeq

NP_003027.1.

UniGene

Hs.663133

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1MR1	X-ray	2.85	C/D	219-313	[»]
1SBX	X-ray	1.65	A	91-192	[»]

ModBase

Search...

Protein-protein interaction databases

IntAct

P12755. 4 interactions.

Proteomic databases

PRIDE

P12755.

Genome annotation databases

Ensembl

ENSG00000157933. Homo sapiens. [Contig view]

GeneID

6497.

KEGG

hsa:6497.

Organism-specific databases

GeneCards

GC01P002173.

H-InvDB

HIX0028862.

HGNC

HGNC:10896. SKI.

HPA

CAB010449.

MIM

164780. gene.

PharmGKB

PA35796.

GenAtlas

Search...

Phylogenomic databases

HOGENOM

P12755.

HOVERGEN

P12755.

OMA

P12755. NKYKRKA.

Enzyme and pathway databases

Pathway_Interaction_DB

bmppathway. BMP receptor signaling.
smad2_3nuclearpathway. Regulation of nuclear SMAD2/3 signaling.

Reactome

REACT_12034. Signaling by BMP.
REACT_6844. Signaling by TGF beta.

Gene expression databases

ArrayExpress

P12755.

Bgee

P12755.

CleanEx

HS_SKI.

GermOnline

ENSG00000157933. Homo sapiens.

Family and domain databases

InterPro

IPR014890. c-SKI_SMAD_bd.
IPR010919. SAND-like.
IPR003380. Transform_Ski.
[Graphical view]

Gene3D

G3DSA:3.10.390.10. SAND. 1 hit.
G3DSA:3.10.260.20. Transform_Ski. 1 hit.

Pfam

PF08782. c-SKI_SMAD_bind. 1 hit.
PF02437. Ski_Sno. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

25255.

SOURCE

Search...

Entry information

Entry name

SKI_HUMAN

Accession

Primary (citable) accession number: P12755
Secondary accession number(s): Q5SYT7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1989
Last sequence update:	October 1, 1989
Last modified:	June 16, 2009
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)