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Protein

Translation initiation factor eIF-2B subunit delta

Gene

GCD2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as essential component of the translation initiation factor 2B (eIF2-B or GCD complex), which catalyzes the exchange of eukaryotic initiation factor 2 (eIF-2)-bound GDP for GTP and is regulated by phosphorylated eIF-2. It activates the synthesis of GCN4 in yeast under amino acid starvation conditions by suppressing the inhibitory effects of multiple AUG codons present in the leader of GCN4 mRNA. It may promote either repression or activation of GCN4 expression depending on amino acid availability. GCD2 is also required for cell viability. Its function can partially be replaced by GCN3 under normal growth conditions in GCD2-defective mutants, under AA starvation conditions GCN3 is an antagonist (GCN4 translational activator).2 Publications

Miscellaneous

Present with 10300 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • enzyme regulator activity Source: SGD
  • translation initiation factor activity Source: SGD

GO - Biological processi

  • regulation of translational initiation Source: SGD

Keywordsi

Molecular functionInitiation factor, Repressor
Biological processProtein biosynthesis, Translation regulation

Enzyme and pathway databases

BioCyciYEAST:G3O-30795-MONOMER
ReactomeiR-SCE-72731 Recycling of eIF2:GDP

Names & Taxonomyi

Protein namesi
Recommended name:
Translation initiation factor eIF-2B subunit delta
Alternative name(s):
GCD complex subunit GCD2
Guanine nucleotide exchange factor subunit GCD2
eIF-2B GDP-GTP exchange factor subunit delta
Gene namesi
Name:GCD2
Synonyms:TIF224
Ordered Locus Names:YGR083C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR083C
SGDiS000003315 GCD2

Subcellular locationi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00001560722 – 651Translation initiation factor eIF-2B subunit deltaAdd BLAST650

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei106PhosphoserineSequence analysis1
Modified residuei121PhosphothreonineSequence analysis1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP12754
PaxDbiP12754
PRIDEiP12754

PTM databases

iPTMnetiP12754

Interactioni

Subunit structurei

Translation initiation factor 2B (eIF2-B) is composed of five different subunits; alpha (GCN3), beta (GCD7), gamma (GCD1), delta (GCD2) and epsilon (GCD6). A regulatory subcomplex comprising GCN3, GCD7 and GCD2 interacts preferentially with phosphorylated eIF-2 and has no exchange activity in vitro.2 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi33325, 113 interactors
DIPiDIP-2343N
IntActiP12754, 30 interactors
MINTiP12754
STRINGi4932.YGR083C

Structurei

3D structure databases

ProteinModelPortaliP12754
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi6 – 106Arg/Lys-rich (basic)Add BLAST101

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00550000075009
HOGENOMiHOG000176924
InParanoidiP12754
KOiK03680
OMAiCIAMLEA
OrthoDBiEOG092C2LM0

Family and domain databases

InterProiView protein in InterPro
IPR000649 IF-2B-related
IPR037171 NagB/RpiA_transferase-like
PfamiView protein in Pfam
PF01008 IF-2B, 1 hit
SUPFAMiSSF100950 SSF100950, 2 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P12754-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSESEAKSRS ATPPSKAKQA TPTTTAAANG EKKLTNKELK ELKKQEKAAK
60 70 80 90 100
RAAMKQANGI SIEQQQQQAQ MKKEKKQLQR EQQQKREQKQ KNANKKKQNE
110 120 130 140 150
RNVKKSTLFG HLETTEERRA TILALTSAVS SPKTSRITAA GLMVPVVASA
160 170 180 190 200
LSGSNVLTAS SLMPVGPNAS STVSASAPAS TTTTLPASSA ALSAGTSSAS
210 220 230 240 250
TNTPTAIQQE IASSNASDVA KTLASISLEA GEFNVIPGIS SVIPTVLEQS
260 270 280 290 300
FDNSSLISSV KELLLNKDLI HPSILLLTSH LAHYKIVGSI PRCIAMLEVF
310 320 330 340 350
QIVIKDYQTP KGTTLSRNLT SYLSHQIDLL KKARPLSVTM GNAIRWLKQE
360 370 380 390 400
ISLIDPSTPD KAAKKDLCEK IGQFAKEKIE LADQLIIDNA STQIEESTTI
410 420 430 440 450
VTYGSSKVLT ELLLHNAISL KKNIKVIVVD SRPLFEGRKM AETLRNAGVN
460 470 480 490 500
VMYALITSLD TIFNMDVDYV FLGAHSILSN GFLYSRAGTA MLAMSAKRRN
510 520 530 540 550
IPVLVCCESL KFSQRVQLDS VTFNELADPN DLVNIDYENP VERRGNKGAL
560 570 580 590 600
LNQFIKERKF EKKKLAMENK PKGNKIGGKK GSEGESKDAS NEEDSNSKNI
610 620 630 640 650
LDGWQELPSL NIVNILYDLT PPEYIKKVIT EFGALPPSSV PVILREYKGS

A
Length:651
Mass (Da):70,852
Last modified:October 1, 1989 - v1
Checksum:iB188457543CE1AAE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X15658 Genomic DNA Translation: CAA33693.1
Z72868 Genomic DNA Translation: CAA97085.1
BK006941 Genomic DNA Translation: DAA08176.1
PIRiS05809 RGBYD2
RefSeqiNP_011597.1, NM_001181212.1

Genome annotation databases

EnsemblFungiiYGR083C; YGR083C; YGR083C
GeneIDi852974
KEGGisce:YGR083C

Similar proteinsi

Entry informationi

Entry nameiEI2BD_YEAST
AccessioniPrimary (citable) accession number: P12754
Secondary accession number(s): D6VUL5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 1, 1989
Last modified: May 23, 2018
This is version 162 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health