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P12753

- RAD50_YEAST

UniProt

P12753 - RAD50_YEAST

Protein

DNA repair protein RAD50

Gene

RAD50

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 146 (01 Oct 2014)
      Sequence version 1 (01 Oct 1989)
      Previous versions | rss
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    Functioni

    Involved in DNA double-strand break repair (DSBR). The rad50/mre11 complex possesses single-strand endonuclease activity and ATP-dependent double-strand-specific exonuclease activity. Rad50 provides ATP-dependent control of mre11 by unwinding and/or repositioning DNA ends into the mre11 active site.

    Cofactori

    Binds 1 zinc ion per homodimer.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi687 – 6871ZincPROSITE-ProRule annotation
    Metal bindingi690 – 6901ZincPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi34 – 418ATPSequence Analysis

    GO - Molecular functioni

    1. adenylate kinase activity Source: SGD
    2. ATPase activity Source: SGD
    3. ATP binding Source: UniProtKB-KW
    4. double-stranded DNA binding Source: SGD
    5. double-stranded telomeric DNA binding Source: SGD
    6. G-quadruplex DNA binding Source: SGD
    7. nuclease activity Source: InterPro
    8. protein binding Source: IntAct
    9. single-stranded telomeric DNA binding Source: SGD
    10. telomeric DNA binding Source: SGD
    11. zinc ion binding Source: InterPro

    GO - Biological processi

    1. ATP catabolic process Source: GOC
    2. base-excision repair Source: SGD
    3. double-strand break repair via break-induced replication Source: SGD
    4. double-strand break repair via nonhomologous end joining Source: SGD
    5. meiotic DNA double-strand break formation Source: SGD
    6. meiotic DNA double-strand break processing Source: SGD
    7. meiotic nuclear division Source: SGD
    8. mitochondrial double-strand break repair via homologous recombination Source: SGD
    9. negative regulation of endodeoxyribonuclease activity Source: SGD
    10. nucleotide phosphorylation Source: GOC
    11. telomere maintenance Source: SGD
    12. telomere maintenance via recombination Source: SGD

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    DNA damage, DNA repair, Meiosis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciYEAST:G3O-33247-MONOMER.
    ReactomeiREACT_189186. Meiotic recombination.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA repair protein RAD50 (EC:3.6.-.-)
    Alternative name(s):
    153 kDa protein
    Gene namesi
    Name:RAD50
    Ordered Locus Names:YNL250W
    ORF Names:N0872
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIV

    Organism-specific databases

    CYGDiYNL250w.
    SGDiS000005194. RAD50.

    Subcellular locationi

    GO - Cellular componenti

    1. Mre11 complex Source: SGD
    2. nucleoplasm Source: Reactome
    3. nucleus Source: SGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 13121312DNA repair protein RAD50PRO_0000138648Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei469 – 4691Phosphoserine1 Publication
    Modified residuei568 – 5681Phosphothreonine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP12753.
    PaxDbiP12753.
    PeptideAtlasiP12753.

    Expressioni

    Gene expression databases

    GenevestigatoriP12753.

    Interactioni

    Subunit structurei

    Homodimer By similarity. Forms a complex with MRE11.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    MRE11P328294EBI-14700,EBI-11255

    Protein-protein interaction databases

    BioGridi35589. 283 interactions.
    DIPiDIP-2419N.
    IntActiP12753. 17 interactions.
    MINTiMINT-435300.
    STRINGi4932.YNL250W.

    Structurei

    3D structure databases

    ProteinModelPortaliP12753.
    SMRiP12753. Positions 1204-1294.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini640 – 741102Zinc-hookPROSITE-ProRule annotationAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili185 – 347163Sequence AnalysisAdd
    BLAST
    Coiled coili403 – 558156Sequence AnalysisAdd
    BLAST
    Coiled coili640 – 67839Sequence AnalysisAdd
    BLAST
    Coiled coili712 – 74130Sequence AnalysisAdd
    BLAST
    Coiled coili787 – 1108322Sequence AnalysisAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi1204 – 124138Ala/Asp-rich (DA-box)Add
    BLAST

    Domaini

    The zinc-hook, which separates the large intramolecular coiled coil regions, contains 2 Cys residues that coordinate one molecule of zinc with the help of the 2 Cys residues of the zinc-hook of another RAD50 molecule, thereby forming a V-shaped homodimer.By similarity

    Sequence similaritiesi

    Belongs to the SMC family. RAD50 subfamily.Curated
    Contains 1 zinc-hook domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiCOG0419.
    GeneTreeiENSGT00390000018781.
    HOGENOMiHOG000192269.
    KOiK10866.
    OMAiPEYIYCL.
    OrthoDBiEOG74BK1D.

    Family and domain databases

    Gene3Di3.40.50.300. 3 hits.
    InterProiIPR027417. P-loop_NTPase.
    IPR004584. Rad50_eukaryotes.
    IPR007517. Rad50_Zn_hook.
    IPR013134. Zn_hook_Rad50.
    [Graphical view]
    PANTHERiPTHR18867. PTHR18867. 1 hit.
    PfamiPF04423. Rad50_zn_hook. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 3 hits.
    TIGRFAMsiTIGR00606. rad50. 1 hit.
    PROSITEiPS51131. ZN_HOOK. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P12753-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSAIYKLSIQ GIRSFDSNDR ETIEFGKPLT LIVGMNGSGK TTIIECLKYA     50
    TTGDLPPNSK GGVFIHDPKI TGEKDIRAQV KLAFTSANGL NMIVTRNIQL 100
    LMKKTTTTFK TLEGQLVAIN NSGDRSTLST RSLELDAQVP LYLGVPKAIL 150
    EYVIFCHQED SLWPLSEPSN LKKKFDEIFQ AMKFTKALDN LKSIKKDMSV 200
    DIKLLKQSVE HLKLDKDRSK AMKLNIHQLQ TKIDQYNEEV SEIESQLNEI 250
    TEKSDKLFKS NQDFQKILSK VENLKNTKLS ISDQVKRLSN SIDILDLSKP 300
    DLQNLLANFS KVLMDKNNQL RDLETDISSL KDRQSSLQSL SNSLIRRQGE 350
    LEAGKETYEK NRNHLSSLKE AFQHKFQGLS NIENSDMAQV NHEMSQFKAF 400
    ISQDLTDTID QFAKDIQLKE TNLSDLIKSI TVDSQNLEYN KKDRSKLIHD 450
    SEELAEKLKS FKSLSTQDSL NHELENLKTY KEKLQSWESE NIIPKLNQKI 500
    EEKNNEMIIL ENQIEKFQDR IMKTNQQADL YAKLGLIKKS INTKLDELQK 550
    ITEKLQNDSR IRQVFPLTQE FQRADLEMDF QKLFINMQKN IAINNKKMHE 600
    LDRRYTNALY NLNTIEKDLQ DNQKSKEKVI QLLSENLPED CTIDEYNDVL 650
    EETELSYKTA LENLKMHQTT LEFNRKALEI AERDSCCYLC SRKFENESFK 700
    SKLLQELKTK TDANFEKTLK DTVQNEKEYL HSLRLLEKHI ITLNSINEKI 750
    DNSQKCLEKA KEETKTSKSK LDELEVDSTK LKDEKELAES EIRPLIEKFT 800
    YLEKELKDLE NSSKTISEEL SIYNTSEDGI QTVDELRDQQ RKMNDSLREL 850
    RKTISDLQME KDEKVRENSR MINLIKEKEL TVSEIESSLT QKQNIDDSIR 900
    SKRENINDID SRVKELEARI ISLKNKKDEA QSVLDKVKNE RDIQVRNKQK 950
    TVADINRLID RFQTIYNEVV DFEAKGFDEL QTTIKELELN KAQMLELKEQ 1000
    LDLKSNEVNE EKRKLADSNN EEKNLKQNLE LIELKSQLQH IESEISRLDV 1050
    QNAEAERDKY QEESLRLRTR FEKLSSENAG KLGEMKQLQN QIDSLTHQLR 1100
    TDYKDIEKNY HKEWVELQTR SFVTDDIDVY SKALDSAIMK YHGLKMQDIN 1150
    RIIDELWKRT YSGTDIDTIK IRSDEVSSTV KGKSYNYRVV MYKQDVELDM 1200
    RGRCSAGQKV LASIIIRLAL SETFGANCGV IALDEPTTNL DEENIESLAK 1250
    SLHNIINMRR HQKNFQLIVI THDEKFLGHM NAAAFTDHFF KVKRDDRQKS 1300
    QIEWVDINRV TY 1312
    Length:1,312
    Mass (Da):152,569
    Last modified:October 1, 1989 - v1
    Checksum:i58A0AA173AC5677E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X14814 Genomic DNA. Translation: CAA32919.1.
    X96722 Genomic DNA. Translation: CAA65494.1.
    Z71526 Genomic DNA. Translation: CAA96157.1.
    BK006947 Genomic DNA. Translation: DAA10309.1.
    PIRiS05808. BWBYDL.
    RefSeqiNP_014149.1. NM_001183088.1.

    Genome annotation databases

    EnsemblFungiiYNL250W; YNL250W; YNL250W.
    GeneIDi855471.
    KEGGisce:YNL250W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X14814 Genomic DNA. Translation: CAA32919.1 .
    X96722 Genomic DNA. Translation: CAA65494.1 .
    Z71526 Genomic DNA. Translation: CAA96157.1 .
    BK006947 Genomic DNA. Translation: DAA10309.1 .
    PIRi S05808. BWBYDL.
    RefSeqi NP_014149.1. NM_001183088.1.

    3D structure databases

    ProteinModelPortali P12753.
    SMRi P12753. Positions 1204-1294.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35589. 283 interactions.
    DIPi DIP-2419N.
    IntActi P12753. 17 interactions.
    MINTi MINT-435300.
    STRINGi 4932.YNL250W.

    Proteomic databases

    MaxQBi P12753.
    PaxDbi P12753.
    PeptideAtlasi P12753.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YNL250W ; YNL250W ; YNL250W .
    GeneIDi 855471.
    KEGGi sce:YNL250W.

    Organism-specific databases

    CYGDi YNL250w.
    SGDi S000005194. RAD50.

    Phylogenomic databases

    eggNOGi COG0419.
    GeneTreei ENSGT00390000018781.
    HOGENOMi HOG000192269.
    KOi K10866.
    OMAi PEYIYCL.
    OrthoDBi EOG74BK1D.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-33247-MONOMER.
    Reactomei REACT_189186. Meiotic recombination.

    Miscellaneous databases

    NextBioi 979418.
    PROi P12753.

    Gene expression databases

    Genevestigatori P12753.

    Family and domain databases

    Gene3Di 3.40.50.300. 3 hits.
    InterProi IPR027417. P-loop_NTPase.
    IPR004584. Rad50_eukaryotes.
    IPR007517. Rad50_Zn_hook.
    IPR013134. Zn_hook_Rad50.
    [Graphical view ]
    PANTHERi PTHR18867. PTHR18867. 1 hit.
    Pfami PF04423. Rad50_zn_hook. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 3 hits.
    TIGRFAMsi TIGR00606. rad50. 1 hit.
    PROSITEi PS51131. ZN_HOOK. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The yeast RAD50 gene encodes a predicted 153-kD protein containing a purine nucleotide-binding domain and two large heptad-repeat regions."
      Alani E., Subbiah S., Kleckner N.
      Genetics 122:47-57(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: RE821.
    2. "Sequence analysis of the 33 kb long region between ORC5 and SUI1 from the left arm of chromosome XIV from Saccharomyces cerevisiae."
      Sen-Gupta M., Gueldener U., Beinhauer J.D., Fiedler T.A., Hegemann J.H.
      Yeast 13:849-860(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 96604 / S288c / FY1679.
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
      Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
      , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
      Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    6. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-469 AND THR-568, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiRAD50_YEAST
    AccessioniPrimary (citable) accession number: P12753
    Secondary accession number(s): D6W0U3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1989
    Last sequence update: October 1, 1989
    Last modified: October 1, 2014
    This is version 146 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 830 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XIV
      Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

    External Data

    Dasty 3