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P12753

- RAD50_YEAST

UniProt

P12753 - RAD50_YEAST

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Protein

DNA repair protein RAD50

Gene

RAD50

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Involved in DNA double-strand break repair (DSBR). The rad50/mre11 complex possesses single-strand endonuclease activity and ATP-dependent double-strand-specific exonuclease activity. Rad50 provides ATP-dependent control of mre11 by unwinding and/or repositioning DNA ends into the mre11 active site.

Cofactori

Binds 1 zinc ion per homodimer.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi687 – 6871ZincPROSITE-ProRule annotation
Metal bindingi690 – 6901ZincPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi34 – 418ATPSequence Analysis

GO - Molecular functioni

  1. adenylate kinase activity Source: SGD
  2. ATPase activity Source: SGD
  3. ATP binding Source: UniProtKB-KW
  4. double-stranded DNA binding Source: SGD
  5. double-stranded telomeric DNA binding Source: SGD
  6. G-quadruplex DNA binding Source: SGD
  7. nuclease activity Source: InterPro
  8. single-stranded telomeric DNA binding Source: SGD
  9. telomeric DNA binding Source: SGD
  10. zinc ion binding Source: InterPro

GO - Biological processi

  1. ATP catabolic process Source: GOC
  2. base-excision repair Source: SGD
  3. double-strand break repair via break-induced replication Source: SGD
  4. double-strand break repair via nonhomologous end joining Source: SGD
  5. meiotic DNA double-strand break formation Source: SGD
  6. meiotic DNA double-strand break processing Source: SGD
  7. meiotic nuclear division Source: SGD
  8. mitochondrial double-strand break repair via homologous recombination Source: SGD
  9. negative regulation of endodeoxyribonuclease activity Source: SGD
  10. nucleotide phosphorylation Source: GOC
  11. telomere maintenance Source: SGD
  12. telomere maintenance via recombination Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

DNA damage, DNA repair, Meiosis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-33247-MONOMER.
ReactomeiREACT_189186. Meiotic recombination.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA repair protein RAD50 (EC:3.6.-.-)
Alternative name(s):
153 kDa protein
Gene namesi
Name:RAD50
Ordered Locus Names:YNL250W
ORF Names:N0872
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIV

Organism-specific databases

CYGDiYNL250w.
SGDiS000005194. RAD50.

Subcellular locationi

GO - Cellular componenti

  1. Mre11 complex Source: SGD
  2. nucleoplasm Source: Reactome
  3. nucleus Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 13121312DNA repair protein RAD50PRO_0000138648Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei469 – 4691Phosphoserine1 Publication
Modified residuei568 – 5681Phosphothreonine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP12753.
PaxDbiP12753.
PeptideAtlasiP12753.

Expressioni

Gene expression databases

GenevestigatoriP12753.

Interactioni

Subunit structurei

Homodimer By similarity. Forms a complex with MRE11.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
MRE11P328294EBI-14700,EBI-11255

Protein-protein interaction databases

BioGridi35589. 283 interactions.
DIPiDIP-2419N.
IntActiP12753. 17 interactions.
MINTiMINT-435300.
STRINGi4932.YNL250W.

Structurei

3D structure databases

ProteinModelPortaliP12753.
SMRiP12753. Positions 1203-1293.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini640 – 741102Zinc-hookPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili185 – 347163Sequence AnalysisAdd
BLAST
Coiled coili403 – 558156Sequence AnalysisAdd
BLAST
Coiled coili640 – 67839Sequence AnalysisAdd
BLAST
Coiled coili712 – 74130Sequence AnalysisAdd
BLAST
Coiled coili787 – 1108322Sequence AnalysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi1204 – 124138Ala/Asp-rich (DA-box)Add
BLAST

Domaini

The zinc-hook, which separates the large intramolecular coiled coil regions, contains 2 Cys residues that coordinate one molecule of zinc with the help of the 2 Cys residues of the zinc-hook of another RAD50 molecule, thereby forming a V-shaped homodimer.By similarity

Sequence similaritiesi

Belongs to the SMC family. RAD50 subfamily.Curated
Contains 1 zinc-hook domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiCOG0419.
GeneTreeiENSGT00390000018781.
HOGENOMiHOG000192269.
InParanoidiP12753.
KOiK10866.
OMAiPEYIYCL.
OrthoDBiEOG74BK1D.

Family and domain databases

Gene3Di3.40.50.300. 3 hits.
InterProiIPR027417. P-loop_NTPase.
IPR004584. Rad50_eukaryotes.
IPR007517. Rad50_Zn_hook.
IPR013134. Zn_hook_Rad50.
[Graphical view]
PANTHERiPTHR18867. PTHR18867. 1 hit.
PfamiPF04423. Rad50_zn_hook. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 3 hits.
TIGRFAMsiTIGR00606. rad50. 1 hit.
PROSITEiPS51131. ZN_HOOK. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P12753-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSAIYKLSIQ GIRSFDSNDR ETIEFGKPLT LIVGMNGSGK TTIIECLKYA
60 70 80 90 100
TTGDLPPNSK GGVFIHDPKI TGEKDIRAQV KLAFTSANGL NMIVTRNIQL
110 120 130 140 150
LMKKTTTTFK TLEGQLVAIN NSGDRSTLST RSLELDAQVP LYLGVPKAIL
160 170 180 190 200
EYVIFCHQED SLWPLSEPSN LKKKFDEIFQ AMKFTKALDN LKSIKKDMSV
210 220 230 240 250
DIKLLKQSVE HLKLDKDRSK AMKLNIHQLQ TKIDQYNEEV SEIESQLNEI
260 270 280 290 300
TEKSDKLFKS NQDFQKILSK VENLKNTKLS ISDQVKRLSN SIDILDLSKP
310 320 330 340 350
DLQNLLANFS KVLMDKNNQL RDLETDISSL KDRQSSLQSL SNSLIRRQGE
360 370 380 390 400
LEAGKETYEK NRNHLSSLKE AFQHKFQGLS NIENSDMAQV NHEMSQFKAF
410 420 430 440 450
ISQDLTDTID QFAKDIQLKE TNLSDLIKSI TVDSQNLEYN KKDRSKLIHD
460 470 480 490 500
SEELAEKLKS FKSLSTQDSL NHELENLKTY KEKLQSWESE NIIPKLNQKI
510 520 530 540 550
EEKNNEMIIL ENQIEKFQDR IMKTNQQADL YAKLGLIKKS INTKLDELQK
560 570 580 590 600
ITEKLQNDSR IRQVFPLTQE FQRADLEMDF QKLFINMQKN IAINNKKMHE
610 620 630 640 650
LDRRYTNALY NLNTIEKDLQ DNQKSKEKVI QLLSENLPED CTIDEYNDVL
660 670 680 690 700
EETELSYKTA LENLKMHQTT LEFNRKALEI AERDSCCYLC SRKFENESFK
710 720 730 740 750
SKLLQELKTK TDANFEKTLK DTVQNEKEYL HSLRLLEKHI ITLNSINEKI
760 770 780 790 800
DNSQKCLEKA KEETKTSKSK LDELEVDSTK LKDEKELAES EIRPLIEKFT
810 820 830 840 850
YLEKELKDLE NSSKTISEEL SIYNTSEDGI QTVDELRDQQ RKMNDSLREL
860 870 880 890 900
RKTISDLQME KDEKVRENSR MINLIKEKEL TVSEIESSLT QKQNIDDSIR
910 920 930 940 950
SKRENINDID SRVKELEARI ISLKNKKDEA QSVLDKVKNE RDIQVRNKQK
960 970 980 990 1000
TVADINRLID RFQTIYNEVV DFEAKGFDEL QTTIKELELN KAQMLELKEQ
1010 1020 1030 1040 1050
LDLKSNEVNE EKRKLADSNN EEKNLKQNLE LIELKSQLQH IESEISRLDV
1060 1070 1080 1090 1100
QNAEAERDKY QEESLRLRTR FEKLSSENAG KLGEMKQLQN QIDSLTHQLR
1110 1120 1130 1140 1150
TDYKDIEKNY HKEWVELQTR SFVTDDIDVY SKALDSAIMK YHGLKMQDIN
1160 1170 1180 1190 1200
RIIDELWKRT YSGTDIDTIK IRSDEVSSTV KGKSYNYRVV MYKQDVELDM
1210 1220 1230 1240 1250
RGRCSAGQKV LASIIIRLAL SETFGANCGV IALDEPTTNL DEENIESLAK
1260 1270 1280 1290 1300
SLHNIINMRR HQKNFQLIVI THDEKFLGHM NAAAFTDHFF KVKRDDRQKS
1310
QIEWVDINRV TY
Length:1,312
Mass (Da):152,569
Last modified:October 1, 1989 - v1
Checksum:i58A0AA173AC5677E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X14814 Genomic DNA. Translation: CAA32919.1.
X96722 Genomic DNA. Translation: CAA65494.1.
Z71526 Genomic DNA. Translation: CAA96157.1.
BK006947 Genomic DNA. Translation: DAA10309.1.
PIRiS05808. BWBYDL.
RefSeqiNP_014149.1. NM_001183088.1.

Genome annotation databases

EnsemblFungiiYNL250W; YNL250W; YNL250W.
GeneIDi855471.
KEGGisce:YNL250W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X14814 Genomic DNA. Translation: CAA32919.1 .
X96722 Genomic DNA. Translation: CAA65494.1 .
Z71526 Genomic DNA. Translation: CAA96157.1 .
BK006947 Genomic DNA. Translation: DAA10309.1 .
PIRi S05808. BWBYDL.
RefSeqi NP_014149.1. NM_001183088.1.

3D structure databases

ProteinModelPortali P12753.
SMRi P12753. Positions 1203-1293.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35589. 283 interactions.
DIPi DIP-2419N.
IntActi P12753. 17 interactions.
MINTi MINT-435300.
STRINGi 4932.YNL250W.

Proteomic databases

MaxQBi P12753.
PaxDbi P12753.
PeptideAtlasi P12753.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YNL250W ; YNL250W ; YNL250W .
GeneIDi 855471.
KEGGi sce:YNL250W.

Organism-specific databases

CYGDi YNL250w.
SGDi S000005194. RAD50.

Phylogenomic databases

eggNOGi COG0419.
GeneTreei ENSGT00390000018781.
HOGENOMi HOG000192269.
InParanoidi P12753.
KOi K10866.
OMAi PEYIYCL.
OrthoDBi EOG74BK1D.

Enzyme and pathway databases

BioCyci YEAST:G3O-33247-MONOMER.
Reactomei REACT_189186. Meiotic recombination.

Miscellaneous databases

NextBioi 979418.
PROi P12753.

Gene expression databases

Genevestigatori P12753.

Family and domain databases

Gene3Di 3.40.50.300. 3 hits.
InterProi IPR027417. P-loop_NTPase.
IPR004584. Rad50_eukaryotes.
IPR007517. Rad50_Zn_hook.
IPR013134. Zn_hook_Rad50.
[Graphical view ]
PANTHERi PTHR18867. PTHR18867. 1 hit.
Pfami PF04423. Rad50_zn_hook. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 3 hits.
TIGRFAMsi TIGR00606. rad50. 1 hit.
PROSITEi PS51131. ZN_HOOK. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The yeast RAD50 gene encodes a predicted 153-kD protein containing a purine nucleotide-binding domain and two large heptad-repeat regions."
    Alani E., Subbiah S., Kleckner N.
    Genetics 122:47-57(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: RE821.
  2. "Sequence analysis of the 33 kb long region between ORC5 and SUI1 from the left arm of chromosome XIV from Saccharomyces cerevisiae."
    Sen-Gupta M., Gueldener U., Beinhauer J.D., Fiedler T.A., Hegemann J.H.
    Yeast 13:849-860(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
    Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
    , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
    Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-469 AND THR-568, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiRAD50_YEAST
AccessioniPrimary (citable) accession number: P12753
Secondary accession number(s): D6W0U3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 1, 1989
Last modified: October 29, 2014
This is version 147 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 830 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

External Data

Dasty 3